Bertha armyworm Mamestra configurata Walker (Lepidoptera: Noctuidae) is a generalist herbivore that feeds on several crops in different plant families. Canola Brassica napus L. (Capparales: Brassicaceae) is one of the most favored host plants making the bertha armyworm a significant insect pest in the Canadian Prairie Provinces. The performance of the bertha armyworm on canola may vary with the quality of the canola plant. We tested the impact of plant nutrition and canola variety on oviposition and subsequent larval development of the bertha armyworm. A range of fertilization levels (0.0,1.0,3.0, and 5.0 g/pot) were applied to three different canola varieties: Clearfield 5535 CL, Roundup Ready hybrid 6060RR, and Q2. The total number of eggs laid on plants in a no-choice experiment increased with fertilizer level but a similar number of eggs were laid on plants that received moderate and high fertilizer treatments. Larvae reared on plants that received the moderate fertilizer application yielded heavier pupae than those reared on plants with low and high fertilizer treatments. Most eggs were laid on the Q2 variety when compared to Clearfield 5535 CL and Roundup Ready 6060 RR. Bertha armyworm females preferred to lay eggs on plants that received moderate or high fertilization over plants with the low fertilizer treatment. Plant leaf tissue nutrient content and plant growth were highest in plants that received moderate and high fertilizer treatment. We conclude that bertha armyworm oviposition behavior and larval performance are influenced by fertilizer treatment and canola variety.

Background: Plants constantly produce primary and secondary metabolites, and a significant fraction of these are volatile organic compounds (VOCs). Factors including the life stage of the plant, temperature, environment, and stress influence the abundance and types of VOCs emitted. The analysis of VOCs released by plants during different stages or with different conditions provides insight into plant metabolism and stress responses. Collecting the VOC profiles of plants in vivo makes it possible to obtain a representative sample of the entire plant volatilome under controlled conditions with minimal invasiveness. In addition, in vivo sampling can also be used to compare the impacts of different environmental conditions or stressors on plants, i.e., the presence/absence of a pest or amount of nitrogen in soil. Methods: In this study, an in vivo plant sampling technique is introduced and validated using active sampling and thermal desorption (TD) tubes with comprehensive two-dimensional gas chromatography coupled to a time-of-flight mass spectrometer (TD-GC×GC-TOFMS). The purpose of this work is to highlight a novel technique to analyze headspace secondary plant metabolites with a minimal invasiveness. Results: It was concluded that in vivo active sampling onto TD tubes provides a wider global coverage of compounds and larger peak areas when compared to extraction by solid-phase microextraction (SPME). Additionally, the Horwitz ratio of active sampling onto TD tubes was 0.893, demonstrating this technique to be a reliable and reproducible method. Lastly, a variety of plants were sampled to assess the versatility of this technique across various plant species with different sizes and volatile profiles. Hundreds of compounds were measured with this analysis, including terpenes, aldehydes, ketones, terpenoids, and alcohols. Conclusions: This novel in vivo active sampling method provides an additional technique for extracting and analyzing volatile secondary plant metabolites.

Orange wheat blossom midge, Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae), has been successfully reared in the laboratory for more than 20 years in Winnipeg, Manitoba, Canada. The rearing method has been developed to the point where it efficiently produces large numbers of wheat midge continuously under laboratory conditions for use in experiments on wheat midge biology and for screening wheat lines for crop resistance. Adult survival was extended by providing high humidity, and oviposition was increased by simulating natural dawn and dusk conditions and by supplying preflowering spring wheat to adults. Preventing desiccation of the wheat midge larvae in the wheat spikes before overwintering in soil and providing optimal cold conditions for a long enough period to break larval diapause enabled successful adult emergence. We provide data to facilitate the coordination of timing of wheat midge emergence from diapause with the wheat susceptible period. The method can be readily scaled up for screening many lines for resistance or scaled down for small experiments. Here, we report details of the rearing method so that others can implement it for research on the management of this internationally important pest.