There is a growing demand for vegan products and plant-based food when dealing with the impact of livestock on the climate crisis. The aim of this study was to develop a formulation for a plant-based analogue of mold-ripened cheese. Were investigated the following plant materials: cashews, pistachios, soy flour, chickpea flour, pea protein, pumpkin protein, hemp protein, and spirulina powder. Plant matrices were fermented with lactic acid bacteria (LAB) starter cultures and cheese starter cultures of mold species Geotrichum candidum and Penicillium camemberti. All microorganisms’ growth were tested in a vegan-type culture medium. Calcium supplementation was applied and followed by an in-depth analysis of the elemental composition of selected analogues with inductively coupled plasma optical emission spectroscopy. The physicochemical and organoleptic analyses of plant-based alternatives of Camembert were conducted. This is the first paper describing novel formulations for plant-based alternatives for Camembert cheese prepared with techniques mimicking the original milk product.

Yarrowia lipolytica is a robust yeast species that has gained significant attention as a biofactory for various biotechnological applications and undoubtedly can be referred to as a hidden treasure trove due to boasting a diverse array of enzymes with wide-ranging applications in multiple industries, including biofuel production, food processing, biotechnology, and pharmaceuticals. As the biotechnology field continues to expand, Y. lipolytica is poised to play a pivotal role in developing eco-friendly and economically viable bioprocesses. Its versatility and potential for large-scale production make it a promising candidate for sustainably addressing various societal and industrial needs. The current review article aimed to highlight the diverse enzymatic capabilities of Y. lipolytica and provide a detailed analysis of its relevance in biocatalysis, including the use of whole-cell catalysts and isolated enzymes. The review focused on wild-type yeast strains and their species-dependant properties and selected relevant examples of Y. lipolytica used as a host organism for overexpressing some enzymes. Furthermore, the application of Y. lipolytica’s potential in enantiomers resolution, lipids processing, and biodiesel synthesis, as well as the synthesis of polymers or esterification of different substrates for upgrading biologically active compounds, was discussed.

To improve enzyme stability, the immobilization process is often applied. The choice of a support on which the enzymes are adsorbed plays a major role in enhancing biocatalysts’ properties. In this study, bio-based (i.e., chitosan, coffee grounds) and synthetic (i.e., Lewatit VP OC 1600) supports were used in the immobilization of lipases of various microbial origins (yeast (Yarrowia lipolytica) and mold (Aspergillus oryzae)). The results confirmed that the enzyme proteins had been adsorbed on the surface of the selected carriers, but not all of them revealed comparably high catalytic activity. Immobilized CALB (Novozym 435) was used as a commercial reference biocatalyst. The best hydrolytic activity (higher than that of CALB) was observed for Novozym 51032 (lipase solution of A. oryzae) immobilized on Lewatit VP OC 1600. In terms of synthetic activity, there were only slight differences between the applied carriers for A. oryzae lipase, and the highest measures were obtained for coffee grounds. All of the biocatalysts had significantly lower activity in the synthesis reactions than the reference catalyst.