Strategic environmental assessment (SEA) can support decision-makers in constructing more sustainable plans, programs, and policies (PPPs). To be more coherent with new frontiers of sustainable cities, PPPs need to include conservation objectives and to increase ecosystem service (ES) strategies. The ES concept is not intrinsic to the SEA process; therefore, it is necessary to develop an approach and methodology to include it. In this paper, we propose a methodology to integrate the concept of ecosystem services in all phases of the SEA process for a sub-urban plan, including the design of mitigation measures. The case study is represented by a peri-urban development plan in the municipality of Gallipoli in South Italy, characterized by a strong tourism economy and valuable agro-ecosystems. The analysis shows the priority ecosystem services that are selected considering the sustainable development and environmental goals, the context of referment, and the aims of the peri-urban plan. After, we highlight the potential ecosystem services developed considering the design of mitigation actions like green infrastructure, which could be implemented in the peri-urban plan. The capacity to develop green infrastructure in SEA processes can configure the SEA as a tool for ecological urban design that is integrated with urban planning. This requires the ability to transfer ecological and planning theories into practical actions and the capacity of different disciplines to work in a transdisciplinary approach.

A study of the distribution of orchid species in Puglia, based on an analysis of 2084 bibliographic reports from 2000 to 2022, was carried out with the aim of revising and updating the information on the consistency of the Orchidaceae family in Puglia, with a special focus on assessing threatened species occurring inside and outside protected areas. The work presents a checklist of the Orchidaceae taxa (genera, species, and subspecies) found in the region, including observations on genera and species that present taxonomic challenges. A total of 113 taxa (i.e., species and subspecies), distributed across 16 genera, are listed in alphabetical order. The most representative genera were Ophrys (51 taxa), Serapias (15 taxa), and Epipactis (11 taxa). Additionally, 49 taxa (43.4%) were found to be endemic to Italy, with 21 of these, mostly belonging to the Ophrys genus, being exclusive to Puglia. Our study notes two different trends of distribution: a predominantly coastal distribution for orchid records located in southern Puglia (the Salento peninsula) and a more widespread distribution for the other provinces. Our study also shows that the greatest number of records locate orchids in protected areas with a positive correlation between their presence and habitats cited in Directive 92/43/EEC.

Many landscapes are the result of interactions between ecological processes, economic activities, and the administrative and political organisation of society. Therefore, as a consequence of human transformations over time, some landscapes may contain residual damaged habitats hosting testimony of past biodiversity that can be called “biodiversity heritage relicts”. From this perspective, the aim of the paper is to describe an applicative approach to habitat restoration in social-ecological landscapes. The approach entails the restoration of vegetation using GIS analysis integrated with field activities and a phytosociological method. The methodology includes expert and stakeholder involvement in order to increase the resilience of the measures over time, thereby consolidating landscape value. The approach was applied in the municipality of Campi Salentina, Province of Lecce, Italy, and the result was the restoration of an important riparian habitat classified under Directive 92/43/EEC as “Salix alba and Populus alba galleries” (code 92A0), which had not previously been recorded in the Province of Lecce. In this case, the project re-established a natural habitat that represented a “biodiversity heritage relict” in the landscape. The paper shows that direct knowledge of the landscape and the ability to identify “biodiversity heritage relicts”, in combination with a phytosociological approach, can enhance the effectiveness of ecological restoration projects. Moreover, social and institutional integration in projects helps ensure the management of the measures over time.

In this study, morphometric analyses were conducted to explore and characterise the differences and analogies between almost all O. fusca s.l. taxa occurring in Apulia. Numerous morphological characters were considered, including 20 continuous variables, 5 ratios and one multistate condition. Our results constitute an exhaustive morphometrical analysis of taxa belonging to the O. fusca group found in Apulia, showing that most of the analysed species are morphologically well defined. Using morphological, phenological and chorological evidence, a new species from southern Italy, Ophrys japigiae sp. nov. is described and illustrated. Some conclusions are drawn with regard to species delimitation, comparing morphology, phenology, chorology and habitat markers.

This study presents an updated analysis of cytogenetic data for several species within the 40,42-chromosome genera of the subtribe Orchidinae. The research includes insights into the distribution of heterochromatin obtained using C-banding and fluorochrome techniques. Our investigation confirmed variation in the distribution of heterochromatin and repetitive DNA sequences among species pertaining to Neotinea s.l. and Orchis s.str. These variations also potentially contribute to the diversification of these species. Cytogenetic analyses of the Neotinea group demonstrated that both H33258 and DAPI staining result in blocks of fluorescent regions on numerous chromosomes. Particular attention was paid to the cytological composition of the polyploid Neotinea commutata, focusing on its potential origin. Based on the karyological results acquired, a hypothesis concerning the origin of N. commutata is proposed. The most noteworthy revelations regard the O. mascula complex. In these species, the telomeric areas of all chromosome sets display extensive heterochromatin. Fluorochrome staining revealed telomeric blocks on many chromosomes that were not seen with Giemsa staining. This highlighted a distinct feature of O. mascula, where particularly large C-bands surrounding the centromeric regions of multiple chromosomes were found. However, in O. mascula, O. provincialis, O. pauciflora, and O. patens, C+ chromatin may not show a significant response to fluorochrome Hoechst or DAPI+ staining. The unique cytomorphological arrangement observed in the O. mascula species, unlike other members of the O. mascula complex, suggest epigenetic phenomena. Additional data are presented for the genera Dactylorhiza and Gymnadenia. A deeper understanding of the diversity of chromosomal structures among these orchids promises to shed light on the mechanisms underlying speciation, adaptation, and the remarkable diversity characteristic of the Orchidaceae family.