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Year of PhD: 2006 University: Corvinus University of Budapest Title of the PhD dissertation: Methods for genetically transforming grape Workplace: National Agricultural Research and Innovation Centre, Research Institute for Viticulture and Oenology Position: scientific advisor Specialty: Grapevine biotechnology
Grapevine virus T (GVT) is a recently described foveavirus, which was identified from a transcriptome of a Teroldego grapevine cultivar in 2017. Recently, we surveyed vineyards and rootstock plantations in Hungary using small RNA (sRNA) high-throughput sequencing (HTS), at a time when GVT had not yet been described. A re-analysis of our sRNA HTS datasets and a survey of grapevines by RT-PCR revealed the presence of GVT in most of the vineyards tested, while at rootstock fields its presence was very rare. The presence and high variability of the virus in the country was confirmed by sequence analysis of strains originating from different vineyards. In this study, we demonstrate the presence of GVT in Hungary and show its high diversity, suggesting that GVT presence may not seriously affect grapevine health and that it could have been present in European vineyards for a long time as a latent infection.
Emese Demian; Aliz Holczbauer; Zsuzsanna Galbacs; Nikoletta Jaksa-Czotter; Mihaly Turcsan; Robert Olah; Eva Varallyay. Variable Populations of Grapevine Virus T Are Present in Vineyards of Hungary. Viruses 2021, 13, 1119 .
AMA StyleEmese Demian, Aliz Holczbauer, Zsuzsanna Galbacs, Nikoletta Jaksa-Czotter, Mihaly Turcsan, Robert Olah, Eva Varallyay. Variable Populations of Grapevine Virus T Are Present in Vineyards of Hungary. Viruses. 2021; 13 (6):1119.
Chicago/Turabian StyleEmese Demian; Aliz Holczbauer; Zsuzsanna Galbacs; Nikoletta Jaksa-Czotter; Mihaly Turcsan; Robert Olah; Eva Varallyay. 2021. "Variable Populations of Grapevine Virus T Are Present in Vineyards of Hungary." Viruses 13, no. 6: 1119.
Meristem culture and somatic embryogenesis are effective tools for virus elimination of vegetatively propagated crops including grapevine (Vitis vinifera L.). While both have been shown to be useful to eliminate the main grapevine viruses, their efficiency differs depending on the virus and grapevine variety. In our work, we investigated the efficiency of these two virus elimination methods using small RNA high-throughput sequencing (HTS) and RT-PCR as virus diagnostics. Field grown mother plants of four clones representing three cultivars, infected with different viruses and viroids, were selected for elimination via somatic embryogenesis (SE) and meristem culture (ME). Our results show for the first time that using SE, elimination in mother plants was effective for all viruses, i.e., grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), Grapevine virus T (GVT) and grapevine Pinot gris virus (GPGV). This study also confirms previous studies showing that SE is a possible strategy for the elimination of GFkV, GRSPaV, HSVd, and GYSVd-1. Our results demonstrate that the efficacy of virus elimination via SE is relatively high while the purging of viroids is lower. Our work provides evidence that the efficiency of SE is comparable to that of the technically difficult ME technique, and that SE will offer a more effective strategy for the production of virus-free grapevine in the future.
Mihaly Turcsan; Emese Demian; Tunde Varga; Nikoletta Jaksa-Czotter; Erno Szegedi; Robert Olah; Eva Varallyay. HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine. Plants 2020, 9, 1782 .
AMA StyleMihaly Turcsan, Emese Demian, Tunde Varga, Nikoletta Jaksa-Czotter, Erno Szegedi, Robert Olah, Eva Varallyay. HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine. Plants. 2020; 9 (12):1782.
Chicago/Turabian StyleMihaly Turcsan; Emese Demian; Tunde Varga; Nikoletta Jaksa-Czotter; Erno Szegedi; Robert Olah; Eva Varallyay. 2020. "HTS-Based Monitoring of the Efficiency of Somatic Embryogenesis and Meristem Cultures Used for Virus Elimination in Grapevine." Plants 9, no. 12: 1782.