This study aimed to investigate the effects of dietary AFB₁ on growth performance, health, intestinal microbiota communities and AFB₁ tissue residues of turbot and evaluate the mitigation efficacy of yeast cell wall extract, Mycosorb^® (YCWE) toward AFB₁ contaminated dietary treatments. Nine experimental diets were formulated: Diet 1 (control): AFB₁ free; Diets 2–5 or Diets 6–9: 20 μg AFB₁/kg diet or 500 μg AFB₁/kg diet + 0%, 0.1%, 0.2%, or 0.4% YCWE, respectively). The results showed that Diet 6 significantly decreased the concentrations of TP, GLB, C3, C4, T-CHO, TG but increased the activities of AST, ALT in serum, decreased the expressions of CAT, SOD, GPx, CYP1A but increased the expressions of CYP3A, GST-ζ₁, p53 in liver. Diet 6 increased the AFB₁ residues in serum and muscle, altered the intestinal microbiota composition, decreased the bacterial community diversity and the abundance of some potential probiotics. However, Diet 8 and Diet 9 restored the immune response, relieved adverse effects in liver, lowered the AFB₁ residues in turbot tissues, promoted intestinal microbiota diversity and lowered the abundance of potentially pathogens. In conclusion, YCWE supplementation decreased the health effects of AFB₁ on turbot, restoring biomarkers closer to the mycotoxin-free control diet.