Photo-curable suspensions are important materials for shaping complex-structured ceramic and glass components. However, most systems undergo slow debinding and sintering so to avoid structural collapse by rapid gas generation from polymers. Here, we propose a new strategy to fabricate interparticle photo-cross-linkable suspensions for rapid three-dimensional structuring with short debinding and sintering times. SiO₂ particles modified with polyethyleneimine complexed with oleic acid (PEI-OA) are dispersed into a refractive-index-tuned solvent, where a photo-radical initiator and a lower-than-typical amount of multifunctional acrylates (MAs) were dissolved. The SiO₂ suspension is cured by the photo-radical polymerization of MA and the Michael additive reaction between polymerized MA and amino groups of PEI-OA on SiO₂ particles. These photo-curable suspensions can be employed in various shaping processes, from micro-scale stereolithography to centimeter-scale silicone molding. The SiO₂ green compacts with complex structures are also debinded and sintered into transparent glass components by rapid heating.

This paper presents a new correlative bioimaging technique using Y₂O₃:Tm, Yb and Y₂O₃:Er, Yb nanophosphors (NPs) as imaging probes that emit luminescence excited by both near-infrared (NIR) light and an electron beam. Under 980 nm NIR light irradiation, the Y₂O₃:Tm, Yb and Y₂O₃:Er, Yb NPs emitted NIR luminescence (NIRL) around 810 nm and 1530 nm, respectively, and cathodoluminescence at 455 nm and 660 nm under excitation of accelerated electrons, respectively. Multimodalities of the NPs were confirmed in correlative NIRL/CL imaging and their locations were visualized at the same observation area in both NIRL and CL images. Using CL microscopy, the NPs were visualized at the single-particle level and with multicolour. Multiscale NIRL/CL bioimaging was demonstrated through in vivo and in vitro NIRL deep-tissue observations, cellular NIRL imaging, and high-spatial resolution CL imaging of the NPs inside cells. The location of a cell sheet transplanted onto the back muscle fascia of a hairy rat was visualized through NIRL imaging of the Y₂O₃:Er, Yb NPs. Accurate positions of cells through the thickness (1.5 mm) of a tissue phantom were detected by NIRL from the Y₂O₃:Tm, Yb NPs. Further, locations of the two types of NPs inside cells were observed using CL microscopy.

A multi-scale direct writing method for metal microstructures is proposed and demonstrated. In this study, metal structures were created in a gelatin matrix containing silver nitrate by photoreduction using a 405-nm blue laser. The influence of concentrations of materials in the sample solution was evaluated by measuring the conductivity of the fabricated microstructures. The fabrication line width could be controlled by changing the laser scanning speed. A network structure was also observed, which possibly helps in increasing the microstructure’s conductivity. Finally, we demonstrated multi-scale drawing by using objective lenses with different numerical apertures. Our method can result in new possibilities for conductive metal direct writing.

The development of handling technology for microscopic biological samples such as cells and spheroids has been required for the advancement of regenerative medicine and tissue engineering. In this study, we developed micro-tweezers with a compliant mechanism to manipulate organoids. The proposed method combines high-resolution microstereolithography that uses a blue laser and topology optimization for shape optimization of micro-tweezers. An actuation system was constructed using a linear motor stage with a force control system to operate the micro-tweezers. The deformation of the topology-optimized micro-tweezers was examined analytically and experimentally. The results verified that the displacement of the tweezer tip was proportional to the applied load; furthermore, the displacement was sufficient to grasp biological samples with an approximate diameter of several hundred micrometers. We experimentally demonstrated the manipulation of an organoid with a diameter of approximately 360 µm using the proposed micro-tweezers. Thus, combining microstereolithography and topology optimization to fabricate micro-tweezers can be potentially used in modifying tools capable of handling various biological samples.