Eucalyptus pellita is known as attractive biomass, and it has been utilized for eucalyptus oil, furniture, and pulp and paper production that causes a significant amount of byproducts. Liquid hot water treatment depending on combined severity factor (CSF) was subjected to isolate hemicellulose fraction from E. pellita and to produce xylooligosaccharides (XOS). The xylan extraction ratio based on the initial xylan content of the feedstock was maximized up to 77.6% at 170 °C for 50 min condition (CSF: 1.0), which had accounted for XOS purity of 76.5% based on the total sugar content of the liquid hydrolysate. In this condition, the sum of xylobiose, xylotriose, and xylotetraose which has a low degree of polymerization (DP) of 2 to 4 was determined as 80.6% of the total XOS. The highest XOS production score established using parameters including the xylan extraction ratio, XOS purity, and low DP XOS ratio was 5.7 at CSF 1.0 condition. XOS production score evaluated using the CSF is expected to be used as a productivity indicator of XOS in the industry (R-squared value: 0.92).

Xylooligosaccharides (XOS) production from sweet sorghum bagasse (SSB) has been barely studied using other edible biomasses. Therefore, we evaluated the XOS content as well as its purity by comparing the content of total sugars from SSB. An environmentally friendly approach involving autohydrolysis was employed, and the reaction temperature and time had variations in order to search for the conditions that would yield high-purity XOS. After autohydrolysis, the remaining solid residues, the glucan-rich fraction, were used as substrates to be enzymatically hydrolyzed for glucose conversion. The highest XOS was observed for total sugars (68.7%) at 190 °C for 5 min among the autohydrolysis conditions. However, we also suggested two alternative conditions, 180 °C for 20 min and 190 °C for 15 min, because the former condition might have the XOS at a low degree of polymerization with a high XOS ratio (67.6%), while the latter condition presented a high glucose to total sugar ratio (91.4%) with a moderate level XOS ratio (64.4%). Although it was challenging to conclude on the autohydrolysis conditions required to obtain the best result of XOS content and purity and glucose yield, this study presented approaches that could maximize the desired product from SSB, and additional processes to reduce these differences in conditions may warrant further research.