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Prof. Diaga Diouf
Université Cheikh Anta Diop de Dakar/Sénégal

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Research Keywords & Expertise

0 mutation breeding
0 Abiotic Stress Tolerance mechanisms
0 Plant genetics and genomics
0 Plant Breeding and genetics
0 plant genetic resources

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Plant Breeding and genetics

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Short Biography

Dr. Diaga Diouf received his PhD in Physiology, Biology and Plant molecular Biology from University Paris 7 Denis Diderot in France. He received post doctoral training in 2002 and 2003 at the Department of Biological Sciences at Virginia Tech and at the Department of Agronomy of University of California at Davis, respectively. He was appointed as an Adjunct professor from 2007 to 2010 at the Department of Biological Sciences at Virginia Tech, in USA. Currently he is a full professor in plant molecular genetics at the university Cheikh Anta Diop in Senegal. His work focuses on understanding the molecular mechanisms that control tolerance of the combined effect of drought and heat in crop using OMIC approaches and bioinformatics tools.

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Journal article
Published: 27 April 2021 in Agronomy
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Meeting food demand for the growing population will require an increase to crop production despite climate changes and, more particularly, severe drought episodes. Sorghum is one of the cereals most adapted to drought that feed millions of people around the world. Valorizing its genetic diversity for crop improvement can benefit from extensive phenotyping. The current methods to evaluate plant biomass, leaves area and plants height involve destructive sampling and are not practical in breeding. Phenotyping relying on drone based imagery is a powerful approach in this context. The objective of this study was to develop and validate a high throughput field phenotyping method of sorghum growth traits under contrasted water conditions relying on drone based imagery. Experiments were conducted in Bambey (Senegal) in 2018 and 2019, to test the ability of multi-spectral sensing technologies on-board a UAV platform to calculate various vegetation indices to estimate plants characteristics. In total, ten (10) contrasted varieties of West African sorghum collection were selected and arranged in a randomized complete block design with three (3) replicates and two (2) water treatments (well-watered and drought stress). This study focused on plant biomass, leaf area index (LAI) and the plant height that were measured weekly from emergence to maturity. Drone flights were performed just before each destructive sampling and images were taken by multi-spectral and visible cameras. UAV-derived vegetation indices exhibited their capacity of estimating LAI and biomass in the 2018 calibration data set, in particular: normalized difference vegetative index (NDVI), corrected transformed vegetation index (CTVI), seconded modified soil-adjusted vegetation index (MSAVI2), green normalize difference vegetation index (GNDVI), and simple ratio (SR) (r 2 of 0.8 and 0.6 for LAI and biomass, respectively). Developed models were validated with 2019 data, showing a good performance (r 2 of 0.92 and 0.91 for LAI and biomass accordingly). Results were also promising regarding plant height estimation (RMSE = 9.88 cm). Regression plots between the image-based estimation and the measured plant height showed a r 2 of 0.83. The validation results were similar between water treatments. This study is the first successful application of drone based imagery for phenotyping sorghum growth and development in a West African context characterized by severe drought occurrence. The developed approach could be used as a decision support tool for breeding programs and as a tool to increase the throughput of sorghum genetic diversity characterization for adaptive traits.

ACS Style

Boubacar Gano; Joseph Dembele; Adama Ndour; Delphine Luquet; Gregory Beurier; Diaga Diouf; Alain Audebert. Using UAV Borne, Multi-Spectral Imaging for the Field Phenotyping of Shoot Biomass, Leaf Area Index and Height of West African Sorghum Varieties under Two Contrasted Water Conditions. Agronomy 2021, 11, 850 .

AMA Style

Boubacar Gano, Joseph Dembele, Adama Ndour, Delphine Luquet, Gregory Beurier, Diaga Diouf, Alain Audebert. Using UAV Borne, Multi-Spectral Imaging for the Field Phenotyping of Shoot Biomass, Leaf Area Index and Height of West African Sorghum Varieties under Two Contrasted Water Conditions. Agronomy. 2021; 11 (5):850.

Chicago/Turabian Style

Boubacar Gano; Joseph Dembele; Adama Ndour; Delphine Luquet; Gregory Beurier; Diaga Diouf; Alain Audebert. 2021. "Using UAV Borne, Multi-Spectral Imaging for the Field Phenotyping of Shoot Biomass, Leaf Area Index and Height of West African Sorghum Varieties under Two Contrasted Water Conditions." Agronomy 11, no. 5: 850.

Journal article
Published: 27 February 2021 in Agronomy
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Sorghum is the fifth most important cereal crop world-wide and feeds millions of people in the Sahel. However, it often faces early-stage water deficit due to false onsets of rainy seasons resulting in production decrease. Therefore, developing early drought tolerant material becomes a necessity but requires a good knowledge of adaptation mechanisms, which remains to be elucidated. The present study aimed at assessing the effects of early drought stress on ten elite sorghum varieties tested over two years (2018–2019) at the National Agronomic Research Centre (CNRA) of Bambey (Senegal, West Africa). Two different water regimes (well-watered and drought stress) were applied during the dry season. Water stress was applied by withholding irrigation 25 days after sowing for one month, followed by optimal irrigation until maturity. Soil moisture measurements were performed and allowed to follow the level of stress (down to a fraction of transpirable soil water (FTSW) of 0.30 at the end of stress). An agro-physio-morphological monitoring was carried out during the experiment. Results showed highly significant effects of early drought stress in sorghum plants growth by decreasing leaf appearance, biomass, height but also yield set up. The combined analysis of variance revealed highly significant differences (p ≤ 0.01) between varieties in the different environments for most characters. Under water deficit, the variability was less strong on leaf appearance and plant height at the end of stress. The adaptation responses were related to the capacity of varieties to grow up fast and complete their cycle rather, increase the dead leaves weight, reduce photosynthesis rate, stomatal conductance, leaf transpiration and increase the roots length density. However, varieties V1, V2, V8 and V9 showed promising behavior under stress and could be suitable for further application in West Africa for sorghum breeding and farming.

ACS Style

Boubacar Gano; Joseph Dembele; Thierry Tovignan; Bassirou Sine; Vincent Vadez; Diaga Diouf; Alain Audebert. Adaptation Responses to Early Drought Stress of West Africa Sorghum Varieties. Agronomy 2021, 11, 443 .

AMA Style

Boubacar Gano, Joseph Dembele, Thierry Tovignan, Bassirou Sine, Vincent Vadez, Diaga Diouf, Alain Audebert. Adaptation Responses to Early Drought Stress of West Africa Sorghum Varieties. Agronomy. 2021; 11 (3):443.

Chicago/Turabian Style

Boubacar Gano; Joseph Dembele; Thierry Tovignan; Bassirou Sine; Vincent Vadez; Diaga Diouf; Alain Audebert. 2021. "Adaptation Responses to Early Drought Stress of West Africa Sorghum Varieties." Agronomy 11, no. 3: 443.

Journal article
Published: 01 January 2021 in BIOCELL
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ACS Style

Made Diouf; Sara Diallo; FRAN荗ISABAYEBADIANE; Oumar Diack; Diaga Diouf. Development of new cowpea (Vigna unguiculata) mutant genotypes, analysis of their agromorphological variation, genetic diversity and population structure. BIOCELL 2021, 45, 345 -362.

AMA Style

Made Diouf, Sara Diallo, FRAN荗ISABAYEBADIANE, Oumar Diack, Diaga Diouf. Development of new cowpea (Vigna unguiculata) mutant genotypes, analysis of their agromorphological variation, genetic diversity and population structure. BIOCELL. 2021; 45 (2):345-362.

Chicago/Turabian Style

Made Diouf; Sara Diallo; FRAN荗ISABAYEBADIANE; Oumar Diack; Diaga Diouf. 2021. "Development of new cowpea (Vigna unguiculata) mutant genotypes, analysis of their agromorphological variation, genetic diversity and population structure." BIOCELL 45, no. 2: 345-362.

Preprint content
Published: 25 May 2020 in bioRxiv
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Cowpea is one of the most important legume grain in the SubSaharian region of Africa used for human consumption and animal feeding but its production is hampered by biotic and abiotic constraints raising the need to broaden its genetic basis. For this purpose, the seeds of two cowpea varieties Melakh and Yacine were respectively irradiated with 300 and 340 Gy. The developed mutant populations were agromorphologically characterized from M5 to M7 while the genetic diversity of the last were evaluated using 13 ISSR markers. Based on agromorphological characterization, variation of flower color, pod length, seed coat color and seed weight with respectively 78.01, 68.29, 94.48 and 57.58% heritability were recorded in the mutant lines. PCA analyses allowed to identify the elite mutants based on their agromorphological traits while Pearson’s correlation results revealed a positive correlation between yield component traits. Three subpopulations were identified through STRUCTURE analyses but assignment of the individuals in each group was improved using DAPC. Analysis of Molecular Variance revealed that the majority (85%) of the variance rather existed within group than among (15%) group. Finally, our study allowed to select new promising mutant genotypes which could be tested for multi local trials to evaluate their agronomic performance.

ACS Style

Made Diouf; Sara Diallo; Francois Abaye Badiane; Oumar Diack; Diaga Diouf. Development of new cowpea (Vigna unguiculata) mutant genotypes, analysis of their agromorphological variation, genetic diversity and Population structure. bioRxiv 2020, 1 .

AMA Style

Made Diouf, Sara Diallo, Francois Abaye Badiane, Oumar Diack, Diaga Diouf. Development of new cowpea (Vigna unguiculata) mutant genotypes, analysis of their agromorphological variation, genetic diversity and Population structure. bioRxiv. 2020; ():1.

Chicago/Turabian Style

Made Diouf; Sara Diallo; Francois Abaye Badiane; Oumar Diack; Diaga Diouf. 2020. "Development of new cowpea (Vigna unguiculata) mutant genotypes, analysis of their agromorphological variation, genetic diversity and Population structure." bioRxiv , no. : 1.

Journal article
Published: 22 September 2018 in Plant Science
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DNA methylation is a heritable epigenetic mechanism that participates in gene regulation under abiotic stresses in plants. Sesame (Sesamum indicum) is typically considered a drought-tolerant crop but highly susceptible to waterlogging, probably because of its origin in Africa or India. Understanding DNA methylation patterns under drought and waterlogging conditions can provide insights into the regulatory mechanisms underlying sesame contrasting responses to these abiotic stresses. We combined Methylation-Sensitive Amplified Polymorphism and transcriptome analyses to profile cytosine methylation patterns, transcript accumulation, and their interplay in drought-tolerant and waterlogging-tolerant sesame genotypes. Drought stress strongly induced de novo methylation (DNM) whereas most of the loci were demethylated (DM) during the recovery phase. In contrast, waterlogging stress decreased the level of methylation but during the recovery phase, both DM and DNM were concomitantly deployed. In both stresses, the levels of the differentially accumulated transcripts (DATs) highly correlated with the methylation patterns. We observed that DM was associated with an increase of DAT levels while DNM was correlated with a decrease of DAT levels. Altogether, sesame has divergent epigenetic programs that respond to drought and waterlogging stresses and an interplay among DNA methylation and transcript accumulation may partly modulate the contrasting responses to these stresses.

ACS Style

Dossa Komivi; Ali Mmadi Marie; Zhou Rong; Zhou Qi; Yang Mei; Cisse Ndiaga; Diouf Diaga; Wang Linhai; Zhang Xiurong. The contrasting response to drought and waterlogging is underpinned by divergent DNA methylation programs associated with transcript accumulation in sesame. Plant Science 2018, 277, 207 -217.

AMA Style

Dossa Komivi, Ali Mmadi Marie, Zhou Rong, Zhou Qi, Yang Mei, Cisse Ndiaga, Diouf Diaga, Wang Linhai, Zhang Xiurong. The contrasting response to drought and waterlogging is underpinned by divergent DNA methylation programs associated with transcript accumulation in sesame. Plant Science. 2018; 277 ():207-217.

Chicago/Turabian Style

Dossa Komivi; Ali Mmadi Marie; Zhou Rong; Zhou Qi; Yang Mei; Cisse Ndiaga; Diouf Diaga; Wang Linhai; Zhang Xiurong. 2018. "The contrasting response to drought and waterlogging is underpinned by divergent DNA methylation programs associated with transcript accumulation in sesame." Plant Science 277, no. : 207-217.

Preprint
Published: 05 July 2018
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DNA methylation is a heritable epigenetic mechanism that participates in gene regulation under abiotic stresses in plants. Sesame (Sesamum indicum L.) is typically considered a drought-tolerant crop but highly susceptible to waterlogging, a property attributed to its presumed origin in Africa or India. Understanding DNA methylation patterns in sesame under drought and waterlogging conditions can provide insights into the regulatory mechanisms underlying its contrasting responses to these principal abiotic stresses. Here, we combined Methylation-Sensitive Amplified Polymorphism and transcriptome analyses to profile cytosine methylation patterns, gene expression alteration, and their interplay in drought-tolerant and waterlogging-tolerant sesame genotypes under control, stress and recovery conditions. Our data showed that drought stress strongly induced de novo methylation (DNM) whereas most of the loci were demethylated (DM) during the recovery phase. In contrast, waterlogging decreased the level of methylation under stress but during the recovery phase, both DM and DNM were concomitantly deployed. In both stresses, the differentially expressed genes (DEGs) were highly correlated with the methylation patterns. We observed that DM was associated with the up-regulation of the DEGs while DNM was correlated with the down-regulation of the DEGs. In addition, we sequenced 44 differentially methylated regions of which 90% overlapped with the promoters and coding sequences of the DEGs. Altogether, we demonstrated that sesame has divergent epigenetic programs that respond to drought and waterlogging stresses. Our results also highlighted the possible interplay among DNA methylation and gene expression, which may modulate the contrasting responses to drought and waterlogging in sesame.

ACS Style

Komivi Dossa; Marie Ali Mmadi; Rong Zhou; Qi Zhou; Mei Yang; Ndiaga Cisse; Diaga Diouf; Linhai Wang; Xiurong Zhang. The contrasting response to drought and waterlogging is underpinned by divergent DNA methylation programs associated with gene expression in sesame. 2018, 362905 .

AMA Style

Komivi Dossa, Marie Ali Mmadi, Rong Zhou, Qi Zhou, Mei Yang, Ndiaga Cisse, Diaga Diouf, Linhai Wang, Xiurong Zhang. The contrasting response to drought and waterlogging is underpinned by divergent DNA methylation programs associated with gene expression in sesame. . 2018; ():362905.

Chicago/Turabian Style

Komivi Dossa; Marie Ali Mmadi; Rong Zhou; Qi Zhou; Mei Yang; Ndiaga Cisse; Diaga Diouf; Linhai Wang; Xiurong Zhang. 2018. "The contrasting response to drought and waterlogging is underpinned by divergent DNA methylation programs associated with gene expression in sesame." , no. : 362905.

Journal article
Published: 01 April 2018 in The Crop Journal
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ACS Style

Komivi Dossa; Xin Wei; Marème Niang; Pan Liu; Yanxin Zhang; Linhai Wang; Boshou Liao; Ndiaga Cissé; Xiurong Zhang; Diaga Diouf. Near-infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia. The Crop Journal 2018, 6, 202 -206.

AMA Style

Komivi Dossa, Xin Wei, Marème Niang, Pan Liu, Yanxin Zhang, Linhai Wang, Boshou Liao, Ndiaga Cissé, Xiurong Zhang, Diaga Diouf. Near-infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia. The Crop Journal. 2018; 6 (2):202-206.

Chicago/Turabian Style

Komivi Dossa; Xin Wei; Marème Niang; Pan Liu; Yanxin Zhang; Linhai Wang; Boshou Liao; Ndiaga Cissé; Xiurong Zhang; Diaga Diouf. 2018. "Near-infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia." The Crop Journal 6, no. 2: 202-206.

Review
Published: 15 February 2018 in World Journal of Microbiology and Biotechnology
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Root endosymbioses are mutualistic interactions between plants and the soil microorganisms (Fungus, Frankia or Rhizobium) that lead to the formation of nitrogen-fixing root nodules and/or arbuscular mycorrhiza. These interactions enable many species to survive in different marginal lands to overcome the nitrogen-and/or phosphorus deficient environment and can potentially reduce the chemical fertilizers used in agriculture which gives them an economic, social and environmental importance. The formation and the development of these structures require the mediation of specific gene products among which the transcription factors play a key role. Three of these transcription factors, viz., CYCLOPS, NSP1 and NSP2 are well conserved between actinorhizal, legume, non-legume and mycorrhizal symbioses. They interact with DELLA proteins to induce the expression of NIN in nitrogen fixing symbiosis or RAM1 in mycorrhizal symbiosis. Recently, the small non coding RNA including micro RNAs (miRNAs) have emerged as major regulators of root endosymbioses. Among them, miRNA171 targets NSP2, a TF conserved in actinorhizal, legume, non-legume and mycorrhizal symbioses. This review will also focus on the recent advances carried out on the biological function of others transcription factors during the root pre-infection/pre-contact, infection or colonization. Their role in nodule formation and AM development will also be described.

ACS Style

Issa Diédhiou; Diaga Diouf. Transcription factors network in root endosymbiosis establishment and development. World Journal of Microbiology and Biotechnology 2018, 34, 37 .

AMA Style

Issa Diédhiou, Diaga Diouf. Transcription factors network in root endosymbiosis establishment and development. World Journal of Microbiology and Biotechnology. 2018; 34 (3):37.

Chicago/Turabian Style

Issa Diédhiou; Diaga Diouf. 2018. "Transcription factors network in root endosymbiosis establishment and development." World Journal of Microbiology and Biotechnology 34, no. 3: 37.

Plant insect interactions
Published: 01 January 2018 in Journal of Plant Interactions
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The cytosine DNA methylation and demethylation have a role in regulating plant responses to the environment by affecting the promoter regions of most plant defense-related genes through the CpG islands or the CCGG motifs. Salicylic acid, a defense and signaling plant hormone, is seen playing crucial role in the variation of the methylome. In this study, the effects of salicylic acid and feeding of the millet headminer (Heliocheilus albipunctella de Joannis) on pearl millet DNA methylome changes were evaluated through MSAP epigenotyping during panicle development. The results showed that millet headminer feeding increased the level of genomic methylation while application of salicylic acid caused DNA demethylation occurring mostly at external cytosine and accompanied by a decrease of the number of larvae per panicle. This suggests that hemimethylation (external cytosine methylation) has key role in regulating defense responses and conferring tolerance to pearl millet through salicylic acid application.

ACS Style

Baba Ngom; Edward Mamati; Mame Fatoumata Goudiaby; Josphert Kimatu; Ibrahima Sarr; Diaga Diouf; Ndjido Ardo Kane. Methylation analysis revealed salicylic acid affects pearl millet defense through external cytosine DNA demethylation. Journal of Plant Interactions 2018, 13, 288 -293.

AMA Style

Baba Ngom, Edward Mamati, Mame Fatoumata Goudiaby, Josphert Kimatu, Ibrahima Sarr, Diaga Diouf, Ndjido Ardo Kane. Methylation analysis revealed salicylic acid affects pearl millet defense through external cytosine DNA demethylation. Journal of Plant Interactions. 2018; 13 (1):288-293.

Chicago/Turabian Style

Baba Ngom; Edward Mamati; Mame Fatoumata Goudiaby; Josphert Kimatu; Ibrahima Sarr; Diaga Diouf; Ndjido Ardo Kane. 2018. "Methylation analysis revealed salicylic acid affects pearl millet defense through external cytosine DNA demethylation." Journal of Plant Interactions 13, no. 1: 288-293.

Journal article
Published: 11 December 2017 in Agronomy
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Sesame is an important crop in West and Central Africa playing a role of an alternative cash crop for smallholders. However, sesame productivity is highly impaired by drought. This study aimed at identifying some drought-resistant genotypes and efficient screening traits in large sesame germplasm. Ten genotypes were examined based on 21 biochemical, physiological, agromorphological and seed quality traits under three weeks of water stress. A high variability for drought resistance was observed among the genotypes. The genotypes WC17, WC18 and WC14 were drought resistant, WC12, WC13, WC06 and WC03 were moderately drought resistant while, WC02, WC10 and WC08 were drought sensitive, based on principal component analysis. The resistant genotypes exhibited both avoidance and tolerance features including increase of the root system, reduced water loss, highest activity of antioxidative enzymes and accumulation of proline. They produced higher biomass and had higher ability to maintain seed quality under drought stress compared with the sensitive genotypes. Strong accumulation (~200% ratio stress/control) of biochemical markers including superoxide dismutase, ascorbate peroxidase, catalase and proline could be regarded as an important indicator for selecting drought resistant genotypes. This study represents a reference for future research towards developing new varieties with improved drought resistance in West and Central Africa.

ACS Style

Komivi Dossa; Louis W. Yehouessi; Benoît C. Likeng-Li-Ngue; Diaga Diouf; Boshou Liao; Xiurong Zhang; Ndiaga Cissé; Joseph M. Bell. Comprehensive Screening of Some West and Central African Sesame Genotypes for Drought Resistance Probing by Agromorphological, Physiological, Biochemical and Seed Quality Traits. Agronomy 2017, 7, 83 .

AMA Style

Komivi Dossa, Louis W. Yehouessi, Benoît C. Likeng-Li-Ngue, Diaga Diouf, Boshou Liao, Xiurong Zhang, Ndiaga Cissé, Joseph M. Bell. Comprehensive Screening of Some West and Central African Sesame Genotypes for Drought Resistance Probing by Agromorphological, Physiological, Biochemical and Seed Quality Traits. Agronomy. 2017; 7 (4):83.

Chicago/Turabian Style

Komivi Dossa; Louis W. Yehouessi; Benoît C. Likeng-Li-Ngue; Diaga Diouf; Boshou Liao; Xiurong Zhang; Ndiaga Cissé; Joseph M. Bell. 2017. "Comprehensive Screening of Some West and Central African Sesame Genotypes for Drought Resistance Probing by Agromorphological, Physiological, Biochemical and Seed Quality Traits." Agronomy 7, no. 4: 83.

Journal article
Published: 18 August 2017 in Scientific Reports
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Sesame is an important oilseed crop with a high oil quality. It is prone to drought stress in the arid and semi-arid areas where it is widely grown. This study aims to decipher the response of tolerant (DT) and sensitive (DS) genotypes to progressive drought based on transcriptome, biochemical and physio-anatomical characterizations. Results indicated that under severe stress, DT relied on a well-functioning taproot while DS displayed a disintegrated root due to collapsed cortical cells. This was attributed to a higher accumulation of osmoprotectants and strong activity of antioxidant enzymes especially peroxidases in DT. From roots, DT could supply water to the aboveground tissues to ensure photosynthetic activities and improve endurance under stress. Temporal transcriptome sequencing under drought further confirmed that DT strongly activated genes related to antioxidant activity, osmoprotection and hormonal signaling pathways including abscisic acid and Ethylene. Furthermore, DT displayed unique differentially expressed genes in root functioning as peroxidases, interleukin receptor-associated kinase, heat shock proteins, APETALA2/ethylene-responsive element-binding protein and mitogen activated protein kinase, to effectively scavenge reactive oxygen species and preserve root cell integrity. Finally, 61 candidate genes conferring higher drought tolerance in DT were discovered and may constitute useful resources for drought tolerance improvement in sesame.

ACS Style

Komivi Dossa; Donghua Li; Linhai Wang; Xiaomin Zheng; Aili Liu; Jingyin Yu; Xin Wei; Rong Zhou; Daniel Foncéka; Diaga Diouf; Boshou Liao; Ndiaga Cissé; Xiurong Zhang. Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes. Scientific Reports 2017, 7, 1 -14.

AMA Style

Komivi Dossa, Donghua Li, Linhai Wang, Xiaomin Zheng, Aili Liu, Jingyin Yu, Xin Wei, Rong Zhou, Daniel Foncéka, Diaga Diouf, Boshou Liao, Ndiaga Cissé, Xiurong Zhang. Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes. Scientific Reports. 2017; 7 (1):1-14.

Chicago/Turabian Style

Komivi Dossa; Donghua Li; Linhai Wang; Xiaomin Zheng; Aili Liu; Jingyin Yu; Xin Wei; Rong Zhou; Daniel Foncéka; Diaga Diouf; Boshou Liao; Ndiaga Cissé; Xiurong Zhang. 2017. "Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes." Scientific Reports 7, no. 1: 1-14.

Review article
Published: 30 June 2017 in Frontiers in Plant Science
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Sesame (Sesamum indicum L.) is one of the oldest oilseed crops widely grown in Africa and Asia for its high-quality nutritional seeds. It is well adapted to harsh environments and constitutes an alternative cash crop for smallholders in developing countries. Despite its economic and nutritional importance, sesame is considered as an orphan crop because it has received very little attention from science. As a consequence, it lags behind the other major oil crops as far as genetic improvement is concerned. In recent years, the scenario has considerably changed with the decoding of the sesame nuclear genome leading to the development of various genomic resources including molecular markers, comprehensive genetic maps, high-quality transcriptome assemblies, web-based functional databases and diverse daft genome sequences. The availability of these tools in association with the discovery of candidate genes and QTLs for key agronomic traits including high oil content and quality, waterlogging and drought tolerance, disease resistance, cytoplasmic male sterility, high yield, pave the way to the development of some new strategies for sesame genetic improvement. As a result, sesame has graduated from an “orphan crop” to a “genomic resource-rich crop”. With the limited research teams working on sesame worldwide, more synergic efforts are needed to integrate these resources in sesame breeding for productivity upsurge, ensuring food security and improved livelihood in developing countries. This review retraces the evolution of sesame research by highlighting the recent advances in the “Omics” area and also critically discusses the future prospects for a further genetic improvement and a better expansion of this crop.

ACS Style

Komivi Dossa; Diaga Diouf; Linhai Wang; Xin Wei; Yanxin Zhang; Mareme Niang; Daniel Fonceka; Jingyin Yu; Marie A. Mmadi; Louis W. Yehouessi; Boshou Liao; Xiurong Zhang; Ndiaga Cisse. The Emerging Oilseed Crop Sesamum indicum Enters the “Omics” Era. Frontiers in Plant Science 2017, 8, 1154 .

AMA Style

Komivi Dossa, Diaga Diouf, Linhai Wang, Xin Wei, Yanxin Zhang, Mareme Niang, Daniel Fonceka, Jingyin Yu, Marie A. Mmadi, Louis W. Yehouessi, Boshou Liao, Xiurong Zhang, Ndiaga Cisse. The Emerging Oilseed Crop Sesamum indicum Enters the “Omics” Era. Frontiers in Plant Science. 2017; 8 ():1154.

Chicago/Turabian Style

Komivi Dossa; Diaga Diouf; Linhai Wang; Xin Wei; Yanxin Zhang; Mareme Niang; Daniel Fonceka; Jingyin Yu; Marie A. Mmadi; Louis W. Yehouessi; Boshou Liao; Xiurong Zhang; Ndiaga Cisse. 2017. "The Emerging Oilseed Crop Sesamum indicum Enters the “Omics” Era." Frontiers in Plant Science 8, no. : 1154.

Original research article
Published: 17 May 2017 in Frontiers in Plant Science
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Pearl millet (Pennisetum glaucum (L.) R. Br.) is a staple food and a drought-tolerant cereal well adapted to Sub-Saharan Africa agro-ecosystems. An important diversity of pearl millet landraces has been widely conserved by farmers and therefore could help copping with climate changes and contribute to future food security. Hence, characterizing its genetic diversity and population structure can contribute to better assist breeding programs for a sustainable agricultural productivity enhancement. Toward this goal, a comprehensive panel of 404 accessions were used that correspond to 12 improved varieties, 306 early-flowering and 86 late-flowering cultivated landraces from Senegal. Twelve highly polymorphic SSR markers were used to study diversity and population structure. Two genes, PgMADS11 and PgPHYC, were genotyped to assess their association to flowering phenotypic difference in landraces. Results indicate a large diversity and untapped potential of Senegalese pearl millet germplasm as well as a genetic differentiation between early- and late-flowering landraces. Further, a fine-scale genetic difference of PgPHYC and PgMADS11 (SNP and indel, respectively) and co-variation of their alleles with flowering time were found among landraces. These findings highlight new genetic insights of pearl millet useful to define heterotic populations for breeding, genomic association panel, or crosses for trait-specific mapping.

ACS Style

Oumar Diack; Ndjido A. Kane; Cecile Berthouly-Salazar; Mame C. Gueye; Baye M. Diop; Amadou Fofana; Ousmane Sy; Hamidou Tall; Leila Zekraoui; Marie Piquet; Marie Couderc; Yves Vigouroux; Diaga Diouf; Adeline Barnaud. New Genetic Insights into Pearl Millet Diversity As Revealed by Characterization of Early- and Late-Flowering Landraces from Senegal. Frontiers in Plant Science 2017, 8, 818 .

AMA Style

Oumar Diack, Ndjido A. Kane, Cecile Berthouly-Salazar, Mame C. Gueye, Baye M. Diop, Amadou Fofana, Ousmane Sy, Hamidou Tall, Leila Zekraoui, Marie Piquet, Marie Couderc, Yves Vigouroux, Diaga Diouf, Adeline Barnaud. New Genetic Insights into Pearl Millet Diversity As Revealed by Characterization of Early- and Late-Flowering Landraces from Senegal. Frontiers in Plant Science. 2017; 8 ():818.

Chicago/Turabian Style

Oumar Diack; Ndjido A. Kane; Cecile Berthouly-Salazar; Mame C. Gueye; Baye M. Diop; Amadou Fofana; Ousmane Sy; Hamidou Tall; Leila Zekraoui; Marie Piquet; Marie Couderc; Yves Vigouroux; Diaga Diouf; Adeline Barnaud. 2017. "New Genetic Insights into Pearl Millet Diversity As Revealed by Characterization of Early- and Late-Flowering Landraces from Senegal." Frontiers in Plant Science 8, no. : 818.

Data article
Published: 27 January 2017 in Genomics Data
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Drought is one of the most important abiotic stresses that impair sesame (Sesamum indicum L.) productivity mainly when it occurs at flowering stage. However up to now, very few studies have attempted to investigate the molecular responses of sesame to drought stress. In this experiment, two genotypes having contrasting responses to drought (tolerant and sensitive) were submitted to progressive drought followed by recovering stage at flowering stage. RNAs were isolated from roots of plants before drought stress, at 3-time points during progressive drought, after rewatering, and sequenced using Illumina HiSeq 4000 platform. These RNA-Seq resources (BioSample IDs: SAMN06130606 and SAMN06130607) provided an opportunity to elucidate the molecular responses of sesame to drought and find out some candidate genes for drought tolerance improvement.

ACS Style

Komivi Dossa; Donghua Li; Linhai Wang; Xiaomin Zheng; Jingyin Yu; Xin Wei; Daniel Fonceka; Diaga Diouf; Boshou Liao; Ndiaga Cisse; Xiurong Zhang. Dynamic transcriptome landscape of sesame ( Sesamum indicum L.) under progressive drought and after rewatering. Genomics Data 2017, 11, 122 -124.

AMA Style

Komivi Dossa, Donghua Li, Linhai Wang, Xiaomin Zheng, Jingyin Yu, Xin Wei, Daniel Fonceka, Diaga Diouf, Boshou Liao, Ndiaga Cisse, Xiurong Zhang. Dynamic transcriptome landscape of sesame ( Sesamum indicum L.) under progressive drought and after rewatering. Genomics Data. 2017; 11 ():122-124.

Chicago/Turabian Style

Komivi Dossa; Donghua Li; Linhai Wang; Xiaomin Zheng; Jingyin Yu; Xin Wei; Daniel Fonceka; Diaga Diouf; Boshou Liao; Ndiaga Cisse; Xiurong Zhang. 2017. "Dynamic transcriptome landscape of sesame ( Sesamum indicum L.) under progressive drought and after rewatering." Genomics Data 11, no. : 122-124.

Original research article
Published: 13 October 2016 in Frontiers in Plant Science
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Sesame is a survivor crop cultivated for ages in arid areas under high temperatures and limited water conditions. Since its entire genome has been sequenced, revealing evolution and functional characterization of its abiotic stress genes became a hot topic. In this study, we performed a whole-genome identification and analysis of Hsf gene family in sesame. Thirty genes encoding Hsf domain were found and classified into 3 major classes A, B and C. The class A members were the most representative one and Hsf genes were distributed in 12 of the 16 linkage groups (except the LG 8, 9, 13 and 16). Evolutionary analysis revealed that, segmental duplication events which occurred around 67 MYA, were the primary force underlying Hsf genes expansion in sesame. Comparative analysis also suggested that sesame has retained most of its Hsf genes while its relatives viz. tomato and potato underwent extensive gene losses during evolution. Continuous purifying selection has played a key role in the maintenance of Hsf genes in sesame. Expression analysis of the Hsf genes in sesame revealed their putative involvement in multiple tissue-/developmental stages. Time-course expression profiling of Hsf genes in response to drought stress showed that 90% Hsfs are drought responsive. We infer that classes B-Hsfs might be the primary regulators of drought response in sesame by cooperating with some class A genes. This is the first insight into this gene family and the results provide some gene resources for future gene cloning and functional studies towards the improvement in stress tolerance of sesame.

ACS Style

Komivi Dossa; Diaga Diouf; Ndiaga Cissé. Genome-Wide Investigation of Hsf Genes in Sesame Reveals Their Segmental Duplication Expansion and Their Active Role in Drought Stress Response. Frontiers in Plant Science 2016, 7, 1522 .

AMA Style

Komivi Dossa, Diaga Diouf, Ndiaga Cissé. Genome-Wide Investigation of Hsf Genes in Sesame Reveals Their Segmental Duplication Expansion and Their Active Role in Drought Stress Response. Frontiers in Plant Science. 2016; 7 ():1522.

Chicago/Turabian Style

Komivi Dossa; Diaga Diouf; Ndiaga Cissé. 2016. "Genome-Wide Investigation of Hsf Genes in Sesame Reveals Their Segmental Duplication Expansion and Their Active Role in Drought Stress Response." Frontiers in Plant Science 7, no. : 1522.

Journal article
Published: 06 July 2016 in African Journal of Biotechnology
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Sesame (Sesamum indicum L.) is one of the most important oilseed crops. It is mainly grown in arid and semi-arid regions with occurrence of unpredictable drought which is one of the major constraints of its production. However, the lack of gene resources associated with drought tolerance hinders sesame genetic improvement towards this osmotic stress. The present research aimed at identifying candidate genes associated with drought tolerance in the whole genome of sesame through homology search of known drought associated genes from three relative species, viz., potato, tomato and the well-described model plant Arabidopsis. Based on 2,495 sequences including 1,150 from Arabidopsis, 1,075 from potato and 270 from tomato, comparative analysis against sesame genome led to the identification of a set of 75 candidate genes (42, 22 and 11 from Arabidopsis, potato and tomato, respectively). Mapping results showed that the candidate genes were distributed on the 16 sesame linkage groups. Wide range of genes with various functions identified in this study confirmed that drought tolerance in sesame is under the control of several genes. Based on their functional classification, 2 groups of candidate genes were identified: (a) genes which protect the plant against drought effect; (b) signal transduction genes and transcription factors. Many transcription factors were retrieved including 6 AP2/ERF genes among which 3 were more expressed in drought tolerant material compared to the sensitive one and might play some important roles in drought tolerance in sesame. Our results provided genomic resources for further functional analysis and genetic engineering towards drought tolerance improvement in sesame. Key words: Sesamum indicum, candidate genes, drought tolerance, orthologous gene, whole genome.

ACS Style

Dossa Komivi; Niang Mareme; E Assogbadjo Achille; Cisse Ndiaga; Diouf Diaga; Komivi Dossa; Mareme Niang; Achille E. Assogbadjo; Ndiaga Cisse; Diaga Diouf. Whole genome homology-based identification of candidate genes for drought tolerance in sesame (Sesamum indicum L.). African Journal of Biotechnology 2016, 15, 1464 -1475.

AMA Style

Dossa Komivi, Niang Mareme, E Assogbadjo Achille, Cisse Ndiaga, Diouf Diaga, Komivi Dossa, Mareme Niang, Achille E. Assogbadjo, Ndiaga Cisse, Diaga Diouf. Whole genome homology-based identification of candidate genes for drought tolerance in sesame (Sesamum indicum L.). African Journal of Biotechnology. 2016; 15 (27):1464-1475.

Chicago/Turabian Style

Dossa Komivi; Niang Mareme; E Assogbadjo Achille; Cisse Ndiaga; Diouf Diaga; Komivi Dossa; Mareme Niang; Achille E. Assogbadjo; Ndiaga Cisse; Diaga Diouf. 2016. "Whole genome homology-based identification of candidate genes for drought tolerance in sesame (Sesamum indicum L.)." African Journal of Biotechnology 15, no. 27: 1464-1475.

Journal article
Published: 12 April 2016 in Genes
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Sesame is an important oil crop widely cultivated in Africa and Asia. Understanding the genetic diversity of accessions from these continents is critical to designing breeding methods and for additional collection of sesame germplasm. To determine the genetic diversity in relation to geographical regions, 96 sesame accessions collected from 22 countries distributed over six geographic regions in Africa and Asia were genotyped using 33 polymorphic SSR markers. Large genetic variability was found within the germplasm collection. The total number of alleles was 137, averaging 4.15 alleles per locus. The accessions from Asia displayed more diversity than those from Africa. Accessions from Southern Asia (SAs), Eastern Asia (EAs), and Western Africa (WAf) were highly diversified, while those from Western Asia (WAs), Northern Africa (NAf), and Southeastern Africa (SAf) had the lowest diversity. The analysis of molecular variance revealed that more than 44% of the genetic variance was due to diversity among geographic regions. Five subpopulations, including three in Asia and two in Africa, were cross-identified through phylogenetic, PCA, and STRUCTURE analyses. Most accessions clustered in the same population based on their geographical origins. Our results provide technical guidance for efficient management of sesame genetic resources in breeding programs and further collection of sesame germplasm from these different regions.

ACS Style

Komivi Dossa; Xin Wei; Yanxin Zhang; Daniel Fonceka; Wenjuan Yang; Diaga Diouf; Boshou Liao; Ndiaga Cisse; Xiurong Zhang. Analysis of Genetic Diversity and Population Structure of Sesame Accessions from Africa and Asia as Major Centers of Its Cultivation. Genes 2016, 7, 14 .

AMA Style

Komivi Dossa, Xin Wei, Yanxin Zhang, Daniel Fonceka, Wenjuan Yang, Diaga Diouf, Boshou Liao, Ndiaga Cisse, Xiurong Zhang. Analysis of Genetic Diversity and Population Structure of Sesame Accessions from Africa and Asia as Major Centers of Its Cultivation. Genes. 2016; 7 (4):14.

Chicago/Turabian Style

Komivi Dossa; Xin Wei; Yanxin Zhang; Daniel Fonceka; Wenjuan Yang; Diaga Diouf; Boshou Liao; Ndiaga Cisse; Xiurong Zhang. 2016. "Analysis of Genetic Diversity and Population Structure of Sesame Accessions from Africa and Asia as Major Centers of Its Cultivation." Genes 7, no. 4: 14.

Journal article
Published: 12 February 2016 in Agronomy
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In the Sahel zone of West Africa that extends from Senegal to Chad, temperatures can vary from less than 15 °C to 25 °C from November to February. These low temperatures affect the growth, development and yield of rice plants, and therefore constitute a major constraint to rice production in the Sahel. In order to identify rice varieties tolerant to cold stress at different developmental stages, a diverse set of 224 rice germplasm was evaluated for yield and yield-related traits in Ndiaye, Senegal, using three different sowing dates. The first sowing date (October 2010), was chosen so as to expose the rice plants to cold stress at the reproductive stage while the rice crop planted at the second sowing date (January 2011) experienced cold stress at the vegetative stage. The third sowing date (July 2011) was the normal planting date for irrigated rice in the Sahel and it served as the control date when the crop does not experience any cold stress throughout its growth cycle. Among the data collected, significant genetic variation was detected and genotype-by-environment interaction was also significant for the traits. At the vegetative stage, cold stress reduced tillering and plant vigor and delayed flowering but increased yield, whereas at the reproductive stage, aside from delaying flowering, cold stress also inhibited panicle exsertion and reduced panicle length, spikelet fertility, grain filling and strongly reduced yields. Principal Component Analysis and correlation analysis using agro-morphological traits helped to identify genotypes that were tolerant to cold stress at either the vegetative or the reproductive stage and the traits associated with high yield under cold stress at each of these stages. Our results can be used to develop cold tolerant rice varieties adapted to double cropping in the Sahelian zone of West Africa.

ACS Style

Daba Ndour; Diaga Diouf; Isaac Kofi Bimpong; Abdoulaye Sow; Ghislain Kanfany; Baboucarr Manneh. Agro-Morphological Evaluation of Rice (Oryza sativa L.) for Seasonal Adaptation in the Sahelian Environment. Agronomy 2016, 6, 8 .

AMA Style

Daba Ndour, Diaga Diouf, Isaac Kofi Bimpong, Abdoulaye Sow, Ghislain Kanfany, Baboucarr Manneh. Agro-Morphological Evaluation of Rice (Oryza sativa L.) for Seasonal Adaptation in the Sahelian Environment. Agronomy. 2016; 6 (1):8.

Chicago/Turabian Style

Daba Ndour; Diaga Diouf; Isaac Kofi Bimpong; Abdoulaye Sow; Ghislain Kanfany; Baboucarr Manneh. 2016. "Agro-Morphological Evaluation of Rice (Oryza sativa L.) for Seasonal Adaptation in the Sahelian Environment." Agronomy 6, no. 1: 8.

Journal article
Published: 10 December 2014 in BMC Plant Biology
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Trees belonging to the Casuarinaceae and Betulaceae families play an important ecological role and are useful tools in forestry for degraded land rehabilitation and reforestation. These functions are linked to their capacity to establish symbiotic relationships with a nitrogen-fixing soil bacterium of the genus Frankia. However, the molecular mechanisms controlling the establishment of these symbioses are poorly understood. The aim of this work was to identify potential transcription factors involved in the establishment and functioning of actinorhizal symbioses. We identified 202 putative transcription factors by in silico analysis in 40 families in Casuarina glauca (Casuarinaceae) and 195 in 35 families in Alnus glutinosa (Betulaceae) EST databases. Based on published transcriptome datasets and quantitative PCR analysis, we found that 39% and 26% of these transcription factors were regulated during C. glauca and A. glutinosa-Frankia interactions, respectively. Phylogenetic studies confirmed the presence of common key transcription factors such as NSP, NF-YA and ERN-related proteins involved in nodule formation in legumes, which confirm the existence of a common symbiosis signaling pathway in nitrogen-fixing root nodule symbioses. We also identified an actinorhizal-specific transcription factor belonging to the zinc finger C1-2i subfamily we named CgZF1 in C. glauca and AgZF1 in A. glutinosa. We identified putative nodulation-associated transcription factors with particular emphasis on members of the GRAS, NF-YA, ERF and C2H2 families. Interestingly, comparison of the non-legume and legume TF with signaling elements from actinorhizal species revealed a new subgroup of nodule-specific C2H2 TF that could be specifically involved in actinorhizal symbioses. In silico identification, transcript analysis, and phylogeny reconstruction of transcription factor families paves the way for the study of specific molecular regulation of symbiosis in response to Frankia infection.

ACS Style

Issa Diédhiou; Alexandre Tromas; Maïmouna Cissoko; Krystelle Gray; Boris Parizot; Amandine Crabos; Nicole Alloisio; Pascale Fournier; Lorena Carro; Sergio Svistoonoff; Hassen Gherbi; Valérie Hocher; Diaga Diouf; Laurent Laplaze; Antony Champion. Identification of potential transcriptional regulators of actinorhizal symbioses in Casuarina glauca and Alnus glutinosa. BMC Plant Biology 2014, 14, 1 -13.

AMA Style

Issa Diédhiou, Alexandre Tromas, Maïmouna Cissoko, Krystelle Gray, Boris Parizot, Amandine Crabos, Nicole Alloisio, Pascale Fournier, Lorena Carro, Sergio Svistoonoff, Hassen Gherbi, Valérie Hocher, Diaga Diouf, Laurent Laplaze, Antony Champion. Identification of potential transcriptional regulators of actinorhizal symbioses in Casuarina glauca and Alnus glutinosa. BMC Plant Biology. 2014; 14 (1):1-13.

Chicago/Turabian Style

Issa Diédhiou; Alexandre Tromas; Maïmouna Cissoko; Krystelle Gray; Boris Parizot; Amandine Crabos; Nicole Alloisio; Pascale Fournier; Lorena Carro; Sergio Svistoonoff; Hassen Gherbi; Valérie Hocher; Diaga Diouf; Laurent Laplaze; Antony Champion. 2014. "Identification of potential transcriptional regulators of actinorhizal symbioses in Casuarina glauca and Alnus glutinosa." BMC Plant Biology 14, no. 1: 1-13.

Book chapter
Published: 29 August 2014 in Broadening the Genetic Base of Grain Legumes
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Cowpea (Vigna unguiculata (L.) Walp.) is an important warm-season legume grown mostly by the poor farmers in the semiarid tropics for human consumption and animal feeding. The crop was originated from Africa where it was domesticated from its wild progenitor V. unguiculata subsp. unguiculata var spontanea. In addition, single nucleotide polymorphism markers (SNP) analysis suggested different domestication events from East to West Africa or single domestication process in the first region followed by transportation in the second. On the basis of molecular analyses, the genome organization of the crop was intensively studied, leading to the identification of two gene pools and gene flow between cultivated and wild forms or crop to crop can be a threat to the breeding programs. A wide range of biotic (virus, bacteria, fungi, insects, nematodes, and plants) and abiotic (like low phosphorus availability, soil acidity or salinity, drought, and high temperature at night) factors are limiting cowpea production in different parts of the world. To overcome these constraints, diverse programs were implemented for base broadening using interspecific hybridization between cowpea and other members of its genus with limited success because of pre-zygotic and post-zygotic barriers. These failures led the investigators to implement protocols to introduce foreign genes into cowpea. Currently, several genes of interest such as herbicide imazapyr, α-amylase inhibitor 1 (against bruchids), and Cry1Ab and Cry1Ac (against Maruca) have been introduced successfully into commercially important cultivars, and these genes are transmitted in Mendelian fashion. In addition, significant genomic resources and a consensus genetic map where agronomic, growth habit, disease, pest resistance, and other trait loci have been placed and are usable in breeding programs.

ACS Style

François Abaye Badiane; Made Diouf; Diaga Diouf. Cowpea. Broadening the Genetic Base of Grain Legumes 2014, 95 -114.

AMA Style

François Abaye Badiane, Made Diouf, Diaga Diouf. Cowpea. Broadening the Genetic Base of Grain Legumes. 2014; ():95-114.

Chicago/Turabian Style

François Abaye Badiane; Made Diouf; Diaga Diouf. 2014. "Cowpea." Broadening the Genetic Base of Grain Legumes , no. : 95-114.