The interaction between living beings, including men, animals and pathogens, sharing the same environment, should be considered as a unique dynamic system, in which the health of each component is inextricably interconnected and dependent with the others. Nowadays, a new integrated One Health approach is reflecting this interdependence with a holistic view to the ecological system. The One Health approach can be defined as a collaborative and a multidisciplinary effort at local, national and global level to guarantee an optimal healthy status for humans, animals and environment. Strictly related to the One Health concept is to be considered the control of infectious diseases, which have influenced the course of human history. Four different components might be identified as key elements within the ‘One World – One Health’ (OWOH) approach: the geographical component, the ecological one, the human activities and the food‐agricultural ones.

Canine leishmaniasis (CanL) is a zoonotic parasitic disease caused by Leishmania infantum in the Mediterranean area and transmitted by phlebotomine sand fly vectors. The domestic dog is the main reservoir host. The aim of this study was to assess the influence of different individual, environmental and spatial risk factors on the dog exposure to L. infantum and to estimate the seroprevalence among owned and kennel dogs, in the Lazio region (central Italy), where canine leishmaniasis is endemic. In the period 2010–2014, 13,292 sera from kennel and owned dogs were collected by official and private veterinarians. The presence of anti-Leishmania IgG was analysed by indirect fluorescent antibody test (IFAT), using a 1:80 titre cut-off. At the univariable analysis, CanL seropositivity was associated with sex, size, breed, coat length, living with other dogs and forest/semi-natural land cover. At the multivariable analysis, age, ownership and attitude were confirmed as risk factors, being more than 2 years old, owned, and hunting dogs at higher risk. Being a Maremma sheepdog was a protective factor. A true overall seroprevalence of 6.7% (95% CI: 6.2–7.2) was estimated in the whole population while 7.3% (95% CI: 6.8–7.8) was estimated in kennel dogs and 74.3% (95% CI: 70.8–77.6) in owned dogs. The role of kennels as a key component for CanL active and passive surveillance was also highlighted. This study confirmed the endemicity of CanL in the Lazio region and focused some factors that can influence the seropositivity of dogs in a Mediterranean region.

The One Health paradigm recognizes that information on infectious diseases, zoonosis and related predictors in animal populations is essential. Pets live in close contact with humans and interact with wild animals, but the lack of reliable information on pet population size, demography and ownership profiles is a constant worldwide. Reliable data must be made available in order to address proper public health policies regarding the design of surveillance plans, the management of canine welfare and stray dog phenomenon, the control of dog behaviour-related problems, and the livestock/wildlife endangerment. Dog identification & registration (I&R) have become mandatory in most European countries in recent years, but the process is far from being widely accomplished, thus resulting in an underestimation of the real canine population. To date, data on the completeness of Dog Registries is very limited. A cross-sectional survey through 630 face-to-face questionnaires was performed with the aim of investigating the dog population size, demography, ownership profiles, and the I&R rate in central Italy. Logistic regression models investigated risk factors with the following outcome variables: dog presence into the Dog Registry, veterinary care frequency, and dog ownership. The present study identified that the dog population is higher than previously reported in Italy and in Europe, whilst lower compared to countries with a poor Human Development Index (a statistic composite index of life expectancy, education, and per capita income indicators used by United Nation). Almost half of the interviewed people (47%) owned at least one dog, totalling 315 dogs. The mean number of dogs per household was 0.7 and 1.5 per dog-owning household. The Bayesian model estimated 481,294 (95% CI: 470,860–491,978) dogs (90 dogs/km²; human:dog ratio 9.0, 95% CI: 8.8–9.2) suggesting that a high density of people does not limit the dog ownership. Dogs resulted predominant among pets. The majority of dogs were males, crossbred, acquires as a gift, lived in rural areas, outdoors and attended a veterinary visit 1–2 times per year. The percentage of neutered dogs were higher in females (55%) than in males (8%). Only 75.3% (95% CI: 73.6–76.9) of the dogs were correctly identified and registered. The Dog Registry completeness increased during the first decades after its establishment, but no improvement has been made afterwards. The dogs correctly identified and registered were more likely to be purebred, neutered, lived in urban areas and visited a veterinarian frequently. Several strategies are recommended to encourage I&R, including promoting responsible dog ownership, engaging private veterinarians and dog breeders, and establishing an effective control system. The present study identified also that the dog source and the kind of feeding were variables associated with the veterinary care frequency. Owning a dog was associated with living in rural areas and the presence of children. The present study reported the poor presence of reliable predictors for the dog ownership. This is the first study to provide an estimate of the canine population abundance, characteristics, and ownership profiles in a European large metropolitan area.

Guaranteeing high acaricide efficacy to control Varroa destructor is fundamental for colony survival. In this study, we verified the efficacy and impact of a commercial thymol-based veterinary product (Apiguard®) on colony honey bee populations when used alone or combined with the biotechnical method of caging honey bee queens to create an artificial brood interruption period in the colony. Apiguard® killed 76.1% of the mites while queen caging killed 40.6% of the mites. The combination of Apiguard® administration with queen caging killed 96.8% of the mites. Comparing bee numbers before and after treatment, Apiguard® treated colonies with caged queens had 48.7% fewer bees compared to before treatment, while Apiguard® alone reduced the number of adult bees by 13.6%. None of the treatments in the different groups resulted in elevated queen mortality.

Intestinal and respiratory parasites are among the most common pathogens in dogs and some of them are recognized as zoonotic agents. In Italy, various taxa have been reported, with variable prevalence estimates depending on study area, dog category and coprological exam technique. In this paper, we report the results of six years of passive surveillance. In the period January 2006-December 2012, 2,775 dog faecal samples from Lazio Region (Central Italy), were examined for parasites, 1,156 from household and 1,619 from shelter dogs, respectively. The following parasites were detected: Giardia duodenalis, Cystoisospora sp., Ancylostomatidae, Toxocara canis, Toxascaris leonina, Trichuris vulpis, Eucoleus böhmi, Eucoleus aerophilus, Mesocestoides sp., Taeniidae and Dipylidium caninum. Helminths were more frequent than protozoa, with total prevalences of 29.1% and 10.7%, respectively. T. vulpis and Ancylostomatidae were the most common parasites, with prevalences of 9.9% and 9.6% respectively. T. vulpis and Ancylostomatidae were significantly more prevalent in shelter dogs than in household ones. T. canis and Cystoisospora sp. were significantly more frequent in household dogs. The assessment of the prevalence in sheltered and in household dogs is useful to infer the occurrence of different parasites in the origin population and to plan possible control intervention.