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With a growing world population, accelerating climate changes, and limited arable land, it is critical to focus on plant-based resources for sustainable food production. In addition, plants are a cornucopia for secondary metabolites, of which many have robust antioxidative capacities and are beneficial for human health. Potato is one of the major food crops worldwide, and is recognized by the United Nations as an excellent food source for an increasing world population. Potato tubers are rich in a plethora of antioxidants with an array of health-promoting effects. This review article provides a detailed overview about the biosynthesis, chemical and health-promoting properties of the most abundant antioxidants in potato tubers, including several vitamins, carotenoids and phenylpropanoids. The dietary contribution of diverse commercial and primitive cultivars are detailed and document that potato contributes much more than just complex carbohydrates to the diet. Finally, the review provides insights into the current and future potential of potato-based systems as tools and resources for healthy and sustainable food production.
Hanjo Hellmann; Aymeric Goyer; Duroy Navarre. Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide. Molecules 2021, 26, 2446 .
AMA StyleHanjo Hellmann, Aymeric Goyer, Duroy Navarre. Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide. Molecules. 2021; 26 (9):2446.
Chicago/Turabian StyleHanjo Hellmann; Aymeric Goyer; Duroy Navarre. 2021. "Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide." Molecules 26, no. 9: 2446.
This chapter is dedicated to the memory of Pierre Gadal a former Series Editor for Advances in Botanical Research. Its aim is to describe the research that has been done in his laboratory covering essentially the period 1970–2000 and provides first-hand testimony about how it has been done. Besides the scientific output concerning CO2 and nitrogen assimilation and their regulation described in this chapter Pierre Gadal has been very influential in plant science in France through his interactions with the University system but also with CNRS and INRA communities. Through the organization of the doctorate school in University of Paris-Sud he has helped train hundreds of students, and more specifically concerning his own research group, more than 10 of his former students have become CNRS researchers, many with high positions and responsibilities, a similar number INRA researchers and Professors and Assistant Professors in the University system. Fourteen different colleagues have contributed to this chapter, each with his own perception, heart and guts, a further testimony to the exceptional human and scientific qualities of Pierre.
Ridong Chen; Michel Droux; Aymeric Goyer; Bertrand Hirel; Michael Hodges; Emmanuelle Issakidis-Bourguet; Jean-Pierre Jacquot; Frederic De Lamotte; Stephane D. Lemaire; Martine Lemaire-Chamley; Myroslawa Miginiac-Maslow; Tatsuo Sugiyama; Akira Suzuki; Jean Vidal. Scientific contributions of Pierre Gadal and his lab—A tribute to Pierre Gadal (1938–2019). Advances in Botanical Research 2021, 1 .
AMA StyleRidong Chen, Michel Droux, Aymeric Goyer, Bertrand Hirel, Michael Hodges, Emmanuelle Issakidis-Bourguet, Jean-Pierre Jacquot, Frederic De Lamotte, Stephane D. Lemaire, Martine Lemaire-Chamley, Myroslawa Miginiac-Maslow, Tatsuo Sugiyama, Akira Suzuki, Jean Vidal. Scientific contributions of Pierre Gadal and his lab—A tribute to Pierre Gadal (1938–2019). Advances in Botanical Research. 2021; ():1.
Chicago/Turabian StyleRidong Chen; Michel Droux; Aymeric Goyer; Bertrand Hirel; Michael Hodges; Emmanuelle Issakidis-Bourguet; Jean-Pierre Jacquot; Frederic De Lamotte; Stephane D. Lemaire; Martine Lemaire-Chamley; Myroslawa Miginiac-Maslow; Tatsuo Sugiyama; Akira Suzuki; Jean Vidal. 2021. "Scientific contributions of Pierre Gadal and his lab—A tribute to Pierre Gadal (1938–2019)." Advances in Botanical Research , no. : 1.
The role of small secreted peptides in plant defense responses to viruses has been seldom investigated. Here, we report a role for potato (Solanum tuberosum) PIP1, a gene predicted to encode a member of the PAMP-induced peptide (PIP) family, in the response of potato to potato virus Y infection (PVY). We show that exogenous application of synthetic StPIP1 to potato leaves and nodes increased the production of reactive oxygen species and the expression of plant defense-related genes, showing that StPIP1 triggers early defense responses. In support of this hypothesis, transgenic potato plants that constitutively overexpress StPIP1 had higher levels of leaf callose deposition, and based on measurements of viral RNA titers, were less susceptible to infection by a compatible PVY strain. Interestingly, systemic infection of StPIP1-overexpressing lines with PVY resulted in clear rugose mosaic symptoms that were absent or very mild in infected non-transgenic plants. A transcriptomics analysis revealed that marker genes associated with both pattern-triggered immunity and effector-triggered immunity were induced in infected StPIP1-overexpressors but not in non-transgenic plants. Together, our results reveal a role for StPIP1 in eliciting plant defense responses and in regulating plant anti-viral immunity.
Max M Combest; Natalia Moroz; Kiwamu Tanaka; Conner J Rogan; Jeffrey C Anderson; Lin Thura; Aurélie M Rakotondrafara; Aymeric Goyer. StPIP1, a PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with potato virus Y. Journal of Experimental Botany 2021, 1 .
AMA StyleMax M Combest, Natalia Moroz, Kiwamu Tanaka, Conner J Rogan, Jeffrey C Anderson, Lin Thura, Aurélie M Rakotondrafara, Aymeric Goyer. StPIP1, a PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with potato virus Y. Journal of Experimental Botany. 2021; ():1.
Chicago/Turabian StyleMax M Combest; Natalia Moroz; Kiwamu Tanaka; Conner J Rogan; Jeffrey C Anderson; Lin Thura; Aurélie M Rakotondrafara; Aymeric Goyer. 2021. "StPIP1, a PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with potato virus Y." Journal of Experimental Botany , no. : 1.
Potato virus Y (PVY; Potyviridae) is a continuing challenge for potato production owing to the increasing popularity of strain-specific resistant cultivars. Hypersensitive resistance (HR) is one type of plant defense responses to restrict virus spread. In many potato cultivars, such as cultivar Premier Russet (PR), local necrosis at the site of infection protects against the most common PVYO strain, but the HR often fails to restrain necrotic strains, which spread systemically. Here, we established the role of callose accumulation in the strain-specific resistance responses to PVY infection. We first uncovered that PVY, independent of the strain, is naturally capable of suppressing pathogenesis-related callose formation in a susceptible host. Such activity can be dissociated from viral replication by the transient expression of the viral-encoded helper component proteinase (HCPro) protein, identifying it as the pathogen elicitor. However, unlike the necrotic strain, PVYO and its corresponding HCPro are unable to block callose accumulation in resistant PR potatoes, in which we observed an abundance of callose deposition and the inability of the virus to spread. The substitution of eight amino acid residues within the HCPro C-terminal region that differ between PVYO and PVYN strains and were previously shown to be responsible for eliciting the HR response, are sufficient to restore the ability of HCProO to suppress callose accumulation, despite the resistant host background, in line with a new viral function in pathogenicity.
Rawnaq N. Chowdhury; Danny Lasky; Hari Karki; Zongying Zhang; Aymeric Goyer; Dennis Halterman; Aurélie M. Rakotondrafara. HCPro Suppression of Callose Deposition Contributes to Strain-Specific Resistance Against Potato Virus Y. Phytopathology® 2020, 110, 164 -173.
AMA StyleRawnaq N. Chowdhury, Danny Lasky, Hari Karki, Zongying Zhang, Aymeric Goyer, Dennis Halterman, Aurélie M. Rakotondrafara. HCPro Suppression of Callose Deposition Contributes to Strain-Specific Resistance Against Potato Virus Y. Phytopathology®. 2020; 110 (1):164-173.
Chicago/Turabian StyleRawnaq N. Chowdhury; Danny Lasky; Hari Karki; Zongying Zhang; Aymeric Goyer; Dennis Halterman; Aurélie M. Rakotondrafara. 2020. "HCPro Suppression of Callose Deposition Contributes to Strain-Specific Resistance Against Potato Virus Y." Phytopathology® 110, no. 1: 164-173.
Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a Solanum boliviense Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers.
Bruce R. Robinson; Carolina Garcia Salinas; Perla Ramos Parra; John Bamberg; Rocio I. Diaz De La Garza; Aymeric Goyer. Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers. Agronomy 2019, 9, 734 .
AMA StyleBruce R. Robinson, Carolina Garcia Salinas, Perla Ramos Parra, John Bamberg, Rocio I. Diaz De La Garza, Aymeric Goyer. Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers. Agronomy. 2019; 9 (11):734.
Chicago/Turabian StyleBruce R. Robinson; Carolina Garcia Salinas; Perla Ramos Parra; John Bamberg; Rocio I. Diaz De La Garza; Aymeric Goyer. 2019. "Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers." Agronomy 9, no. 11: 734.
BACKGROUND Changes in potato tuber’s metabolites composition during low temperature storage can affect the tuber’s nutritional value, its susceptibility to bruising, and its processing qualities. Here, we measured changes in the amounts of folate, vitamin B6, and vitamin C, and the blackspot pigments precursors chlorogenic acid and tyrosine, as well as phenylalanine in five potato varieties stored at 7.8°C for eight months in 2015 and 2016. RESULTS Folate content increased in all varieties in both years during low temperature storage, with statistically significant changes occurring in six out of eight conditions. Increase rates ranged from 11% to 141%. Vitamin B6 content increased in all varieties during the storage period, but changes were statistically significant in only two out of eight conditions. Increase rates ranged from 5% to 24%. Ascorbic acid content decreased in all varieties in both years during the storage period. Decrease rates ranged from 16% to 78%, and were statistically significant in seven out of eight conditions. For chlorogenic acid, no consistent trend was observed. Changes varied between ‐14% and +14%, but none were statistically significant. Tyrosine content increased in all varieties in both years, except in Sage Russet in 2015. Increase rates ranged from 19% to 238% and were statistically significant in three out of seven conditions. Changes in phenylalanine content were very similar to those observed for tyrosine, with increases up to 272% in Teton Russet. CONCLUSIONS These results show that storage at low temperature affects substantially tuber nutritional quality and biochemical bruising potential. This article is protected by copyright. All rights reserved.
Aymeric Goyer; Maël Picard; Hanjo A. Hellmann; Sutton L. Mooney. Effect of low‐temperature storage on the content of folate, vitamin B 6 , ascorbic acid, chlorogenic acid, tyrosine, and phenylalanine in potatoes. Journal of the Science of Food and Agriculture 2019, 99, 4842 -4848.
AMA StyleAymeric Goyer, Maël Picard, Hanjo A. Hellmann, Sutton L. Mooney. Effect of low‐temperature storage on the content of folate, vitamin B 6 , ascorbic acid, chlorogenic acid, tyrosine, and phenylalanine in potatoes. Journal of the Science of Food and Agriculture. 2019; 99 (10):4842-4848.
Chicago/Turabian StyleAymeric Goyer; Maël Picard; Hanjo A. Hellmann; Sutton L. Mooney. 2019. "Effect of low‐temperature storage on the content of folate, vitamin B 6 , ascorbic acid, chlorogenic acid, tyrosine, and phenylalanine in potatoes." Journal of the Science of Food and Agriculture 99, no. 10: 4842-4848.
Micronutrient deficiency, also known as the hidden hunger, affects over two billion people worldwide. Potato is the third most consumed food crops in the world, and is therefore a fundamental element of food security for millions of people. Increasing the amount of micronutrients in food crop could help alleviate worldwide micronutrient malnutrition. In the present study, we report on the identification of single nucleotide polymorphism (SNP) markers associated with folate, an essential micronutrient in the human diet. A high folate diploid clone Fol 1.6 from the wild potato relative Solanum boliviense (PI 597736) was crossed with a low/medium folate diploid S. tuberosum clone USW4self#3. The resulting F1 progeny was intermated to generate an F2 population, and tubers from 94 F2 individuals were harvested for folate analysis and SNP genotyping using a SolCap 12K Potato SNP array. Folate content in the progeny ranged from 304 to 2,952 ng g-1 dry weight. 6,759 high quality SNPs containing 4,174 (62%) polymorphic and 2,585 (38%) monomorphic SNPs were used to investigate marker-trait association. Association analysis was performed using two different approaches: survey SNP-trait association (SSTA) and SNP-trait association (STA). A total of 497 significant SNPs were identified, 489 by SSTA analysis and 43 by STA analysis. Markers identified by SSTA were located on all twelve chromosomes while those identified by STA were confined to chromosomes 2, 4, and 6. Eighteen of the significant SNPs were located within or in close proximity to folate metabolism-related genes. Forty two SNPs were identical between SSTA and STA analyses. These SNPs have potential to be used in marker-assisted selection for breeding high folate potato varieties.
Sapinder Bali; Bruce R. Robinson; Vidyasagar Sathuvalli; John Bamberg; Aymeric Goyer. Single Nucleotide Polymorphism (SNP) markers associated with high folate content in wild potato species. PLOS ONE 2018, 13, e0193415 .
AMA StyleSapinder Bali, Bruce R. Robinson, Vidyasagar Sathuvalli, John Bamberg, Aymeric Goyer. Single Nucleotide Polymorphism (SNP) markers associated with high folate content in wild potato species. PLOS ONE. 2018; 13 (2):e0193415.
Chicago/Turabian StyleSapinder Bali; Bruce R. Robinson; Vidyasagar Sathuvalli; John Bamberg; Aymeric Goyer. 2018. "Single Nucleotide Polymorphism (SNP) markers associated with high folate content in wild potato species." PLOS ONE 13, no. 2: e0193415.
Blackspot in potato is an internal tissue discoloration that occurs during handling and transport of potato tubers. Blackspot is cosmetically undesirable and represents a huge economic cost for the potato industry. The aim of this study was to test whether concentrations of certain metabolites in the potato tuber cortex could predict blackspot susceptibility. Seven russet potato varieties were stored for eight months at 8.80C. Stored tubers were subjected to mechanical impact and evaluated for blackspot susceptibility. A blackspot susceptibility index was calculated for each variety by determining an index for the percentage of the tuber cortex area that was covered with blackspot, and an index for the intensity of blackspot discoloration. Concentrations of tyrosine, chlorogenic acid, phenylalanine, and ascorbic acid, and blackspot biochemical potential of tubers to synthesize pigments, were measured in the tuber cortex. Blackspot indices, metabolites concentrations and blackspot biochemical potential varied significantly between varieties. Tyrosine concentrations strongly, significantly, and positively correlated with blackspot biochemical potential. Phenylalanine concentrations showed good, significant, and positive correlation with blackspot biochemical potential and discoloration index. None of the analyzed metabolites correlated with blackspot susceptibility. Concentrations of tyrosine and phenylalanine explained up to ~80% of the variation in blackspot biochemical potential between varieties, but did not correlate with blackspot susceptibility.
Aymeric Goyer; Julien Pellé. Relationships between tyrosine, phenylalanine, chlorogenic acid, and ascorbic acid concentrations and blackspot biochemical potential and blackspot susceptibility in stored russet potatoes. Journal of the Science of Food and Agriculture 2018, 98, 3735 -3740.
AMA StyleAymeric Goyer, Julien Pellé. Relationships between tyrosine, phenylalanine, chlorogenic acid, and ascorbic acid concentrations and blackspot biochemical potential and blackspot susceptibility in stored russet potatoes. Journal of the Science of Food and Agriculture. 2018; 98 (10):3735-3740.
Chicago/Turabian StyleAymeric Goyer; Julien Pellé. 2018. "Relationships between tyrosine, phenylalanine, chlorogenic acid, and ascorbic acid concentrations and blackspot biochemical potential and blackspot susceptibility in stored russet potatoes." Journal of the Science of Food and Agriculture 98, no. 10: 3735-3740.
Micronutrient malnutrition, also known as the hidden hunger, affects over two billion people worldwide. Potato is the third most consumed food crop in the world and is therefore a fundamental element of food security for millions of people. Increasing the amount of micronutrients in potatoes could help alleviate worldwide micronutrient malnutrition. Folate (or vitamin B9) is an essential micronutrient in the human diet. Deficiencies in folate lead to serious, sometimes lethal, diseases. Unfortunately, folate intake remains suboptimal in both developing and developed regions of the world. This paper uses folate to illustrate various approaches that could be implemented to increase micronutrient content in potato. It provides a brief overview of recent analyses of folate content in diverse potato germplasm, of changes in folate content during tuber development, and of the effect of postharvest low-temperature storage of potato on folate content, and how an optimization of these different factors could lead to significant increases in folate intake from potato.
Aymeric Goyer. Maximizing the Nutritional Potential of Potato: the Case of Folate. Potato Research 2017, 60, 319 -325.
AMA StyleAymeric Goyer. Maximizing the Nutritional Potential of Potato: the Case of Folate. Potato Research. 2017; 60 (3-4):319-325.
Chicago/Turabian StyleAymeric Goyer. 2017. "Maximizing the Nutritional Potential of Potato: the Case of Folate." Potato Research 60, no. 3-4: 319-325.
Thiamin is essential for human health. While plants are the ultimate source of thiamin in most human diets, staple foods like white rice have low thiamin content. Therefore, populations whose diets are mainly based on low-thiamin staple crops suffer from thiamin deficiency. Biofortification of rice grain by engineering the thiamin biosynthesis pathway has recently been attempted, with up to 5-fold increase in thiamin content in unpolished seeds. However, polished seeds that retain only the starchy endosperm had similar thiamin content than that of non-engineered plants. Various factors such as limited supply of precursors, limited activity of thiamin biosynthetic enzymes, dependence on maternal tissues to supply thiamin, or lack of thiamin stabilizing proteins may have hindered thiamin increase in the endosperm.
Aymeric Goyer. Thiamin biofortification of crops. Current Opinion in Biotechnology 2017, 44, 1 -7.
AMA StyleAymeric Goyer. Thiamin biofortification of crops. Current Opinion in Biotechnology. 2017; 44 ():1-7.
Chicago/Turabian StyleAymeric Goyer. 2017. "Thiamin biofortification of crops." Current Opinion in Biotechnology 44, no. : 1-7.
Potato virus Y (PVY) is a major potato pathogen affecting potato yields worldwide. Thiamin, a water-soluble B vitamin (vitamin B) has been shown to boost the plant’s immunity, thereby increasing resistance against pathogens. In this study, we tested different concentrations of thiamin (1 mM, 10 mM, 50 mM, 100 mM) and multiple thiamin applications (once, biweekly and monthly) on potato resistance to PVY in Ranger Russet potatoes. Plants were mechanically inoculated with PVY. This PVY strain is known for causing well-defined foliar symptoms. We collected leaflets weekly through April and May 2015 and tested them with an enzyme-linked immunosorbent assay specific to PVY as well as by real time quantitative RT-PCR. These assays allowed us to determine the presence and level of PVY in different parts of the plants. We found that the highest thiamin concentration treatment (100 mM) produced the lowest virus level in potatoes across all dates and leaflet samples. Also, it was found that multiple applications of thiamin had a positive effect on reducing virus level, especially when thiamin was applied every four weeks.El Virus Y de la Papa (PVY) es un patógeno importante que afecta los rendimientos de la papa mundialmente. Se ha demostrado que la tiamina, una vitamina B soluble en agua (vitamina B1), estimula la inmunidad de la planta, aumentando la resistencia contra patógenos. En este estudio, probamos diferentes concentraciones de tiamina (1 mM, 10 mM, 50 mM, 100 mM) y aplicaciones múltiples (una vez, quincenalmente y mensualmente) sobre la resistencia de la papa al PVY en la variedad Ranger Russet. Las plantas se inocularon mecánicamente con PVY. Esta variante es conocida por causar síntomas foliares bien definidos. Colectamos folíolos semanalmente durante abril y mayo, 2015, y los probamos con un ensayo serológico de enzimas conjugadas específico para PVY, así como con PCR-RT de tiempo real cuantitativo. Estos ensayos nos permitieron determinar la presencia y nivel de PVY en diferentes partes de la planta. Encontramos que el tratamiento con la más alta concentración de tiamina (100 mM) produjo el más bajo nivel de virus en papa a lo largo de todas las fechas y muestras de folíolos. También se encontró que múltiples aplicaciones de tiamina tuvieron un efecto positivo en la reducción del nivel del virus, especialmente cuando la tiamina se aplicó cada cuatro semanas.
Amber C. Vinchesi; Silvia I. Rondon; Aymeric Goyer. Priming Potato with Thiamin to Control Potato Virus Y. American Journal of Potato Research 2016, 94, 120 -128.
AMA StyleAmber C. Vinchesi, Silvia I. Rondon, Aymeric Goyer. Priming Potato with Thiamin to Control Potato Virus Y. American Journal of Potato Research. 2016; 94 (2):120-128.
Chicago/Turabian StyleAmber C. Vinchesi; Silvia I. Rondon; Aymeric Goyer. 2016. "Priming Potato with Thiamin to Control Potato Virus Y." American Journal of Potato Research 94, no. 2: 120-128.
Thiamin diphosphate (ThDP), also known as vitamin B1, serves as an enzymatic cofactor in glucose metabolism, the Krebs cycle, and branched-chain amino acid biosynthesis in all living organisms. Unlike plants and microorganisms, humans are not able to synthesize ThDP de novo and must obtain it from their diet. Staple crops such as rice are poor sources of thiamin. Hence, populations that mainly consume rice commonly suffer thiamin deficiency. In addition to thiamin’s nutritional function, studies in rice have shown that some thiamin biosynthesis genes are involved in resistance to Xanthomonas oryzae, which causes a serious disease in rice fields. This study shows that overexpression of two thiamin biosynthesis genes, 4-methyl-5-β-hydroxyethylthiazole phosphate synthase and 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate synthase, involved in the first steps of the thiazole and pyrimidine synthesis branches, respectively, increased thiamin content up to fivefold in unpolished seeds that retain the bran. However, thiamin levels in polished seeds with removed bran were similar to those found in polished control seeds. Plants with higher accumulation of thiamin did not show enhanced resistance to X. oryzae. These results indicate that stacking of two traits can enhance thiamin accumulation in rice unpolished grain. We discuss potential roadblocks that prevent thiamin accumulation in the endosperm.
Wei Edong; Nicholas Thomas; Pamela C. Ronald; Aymeric Egoyer. Overexpression of Thiamin Biosynthesis Genes in Rice Increases Leaf and Unpolished Grain Thiamin Content But Not Resistance to Xanthomonas oryzae pv. oryzae. Frontiers in Plant Science 2016, 7, 616 .
AMA StyleWei Edong, Nicholas Thomas, Pamela C. Ronald, Aymeric Egoyer. Overexpression of Thiamin Biosynthesis Genes in Rice Increases Leaf and Unpolished Grain Thiamin Content But Not Resistance to Xanthomonas oryzae pv. oryzae. Frontiers in Plant Science. 2016; 7 ():616.
Chicago/Turabian StyleWei Edong; Nicholas Thomas; Pamela C. Ronald; Aymeric Egoyer. 2016. "Overexpression of Thiamin Biosynthesis Genes in Rice Increases Leaf and Unpolished Grain Thiamin Content But Not Resistance to Xanthomonas oryzae pv. oryzae." Frontiers in Plant Science 7, no. : 616.
Malnutrition is one of the world’s largest health concerns. Folate (also known as vitamin B9) is essential in the human diet, and without adequate folate intake, several serious health concerns, such as congenital birth defects and an increased risk of stroke and heart disease, can occur. Most people’s folate intake remains sub-optimal, even in countries that have a folic acid food fortification program in place. Staple crops, such as potatoes, represent an appropriate organism for biofortification through traditional breeding based on their worldwide consumption and the fact that modern cultivars only contain about 6% of the daily recommended intake of folate. To start breeding potatoes with enhanced folate content, high folate potato material must be identified. In this study, 250 individual plants from 77 accessions and 10 Solanum species were screened for their folate content using a tri-enzyme extraction and microbial assay. There was a 10-fold range of folate concentrations among individuals. Certain individuals within the species Solanum tuberosum subsp. andigenum, Solanum vernei and Solanum boliviense have the potential to produce more than double the folate concentrations of commercial cultivars, such as Russet Burbank. Our results show that tapping into the genetic diversity of potato is a promising approach to increase the folate content of this important crop.
Bruce R. Robinson; Vidyasagar Sathuvalli; John Bamberg; Aymeric Goyer. Exploring Folate Diversity in Wild and Primitive Potatoes for Modern Crop Improvement. Genes 2015, 6, 1300 -1314.
AMA StyleBruce R. Robinson, Vidyasagar Sathuvalli, John Bamberg, Aymeric Goyer. Exploring Folate Diversity in Wild and Primitive Potatoes for Modern Crop Improvement. Genes. 2015; 6 (4):1300-1314.
Chicago/Turabian StyleBruce R. Robinson; Vidyasagar Sathuvalli; John Bamberg; Aymeric Goyer. 2015. "Exploring Folate Diversity in Wild and Primitive Potatoes for Modern Crop Improvement." Genes 6, no. 4: 1300-1314.
The bird cherry-oat aphid, Rhopalosiphum padi (L.) (Heteroptera: Aphididae), causes heavy losses to wheat crops worldwide by direct damage and virus transmission. This study was conducted to identify putative resistance mechanisms in four wheat varieties (Bobtail, Ladd, Stephens, and Skiles) and one advanced line (YS434) where R. padi was subjected to choice and no-choice tests. Antixenosis, antibiosis and tolerance studies were conducted in controlled environmental conditions at temperature of 20 ± 5°C, 50–65% RH, and a photoperiod of 14:10 (L:D) h. Based on the antixenosis test, the variety Skiles was found susceptible to R. padi, while the line YS434 showed a significant level of resistance; the varieties Bobtail, Ladd, and Stephens showed intermediate response. In the antibiosis experiment, R. padi produced less progeny on the variety Skiles as compared with other varieties, but the developmental time for nymphs was also significantly shorter on Skiles and recorded higher intrinsic rate of natural increase (rm) values as compared with the varieties YS434, Bobtail, and Ladd. In the tolerance tests, the variety Ladd showed significantly lower tolerance index value than YS434, followed by Skiles, Bobtail, and Stephens. The plant resistance index value was greater for the variety Ladd, followed by Stephens, YS434, and Bobtail. In conclusion, this study provides baseline information that will contribute to the identification of putative resistance factors for a future breeding program against this aphid. RESUMEN: El pulgón del trigo, Rhopalosiphum padi (L.) (Heteroptera: Aphididae), provoca grandes pérdidas en el cultivo de trigo a nivel mundial debido a su comportamiento de alimentación directa o transmitiendo viruses. Este estudio se realizó para categorizar factores de resistencia en cuatro variedades de trigo (Bobtail, Ladd, Stephens y Skiles) y una linea avanzada (YS434) contra este áfido. Se realizaron estudios de antixenosis, antibiosis y tolerancia en un ambiente controlado a temperatura de 20 ± 5°C, humedad relativa 50–65% y un fotoperíodo de 14L:10D. Basado en las pruebas de antixenosis, la variedad Skiles fue susceptible a R. padi, mientras la linea YS434 mostró resistencia; la variedad Bobtail, Ladd and Stephens mostraron respuesta intermedia. En los estudios de antibiosis, R. padi produció menos progenie en la variedad Skiles comparado con las otras variedades; el valor intrinsico natural fue tambien mayor en la misma variedad. En las pruebas de tolerancia, la variedad Ladd mostró un índice de tolerancia bajo, seguido por YS434, Skiles, Bobtail y Stephens. El índice de resistencia fue mayor en la variedad Ladd seguido de Stephens, YS434 y Bobtail. En conclusión, este estudio presenta información que contribuirá en la categorización de factores para el futuro de los programas de mejoramiento genéticos.
Qamar Zeb; Silvia I. Rondon; Mohammad Naeem; Shah Alam Khan; Aymeric Goyer; Steve Van Vleet; Mary K. Corp. Categorization of Putative Factors AgainstRhopalosiphum padi(L.) (Heteroptera: Aphididae). Journal of Economic Entomology 2015, 109, 439 -444.
AMA StyleQamar Zeb, Silvia I. Rondon, Mohammad Naeem, Shah Alam Khan, Aymeric Goyer, Steve Van Vleet, Mary K. Corp. Categorization of Putative Factors AgainstRhopalosiphum padi(L.) (Heteroptera: Aphididae). Journal of Economic Entomology. 2015; 109 (1):439-444.
Chicago/Turabian StyleQamar Zeb; Silvia I. Rondon; Mohammad Naeem; Shah Alam Khan; Aymeric Goyer; Steve Van Vleet; Mary K. Corp. 2015. "Categorization of Putative Factors AgainstRhopalosiphum padi(L.) (Heteroptera: Aphididae)." Journal of Economic Entomology 109, no. 1: 439-444.
Thiamin is an essential nutrient in the human diet. Severe thiamin deficiency leads to beriberi, a lethal disease which is common in developing countries. Thiamin biofortification of staple food crops is a possible strategy to alleviate thiamin deficiency-related diseases. In plants, thiamin plays a role in the response to abiotic and biotic stresses, and data from the literature suggest that boosting thiamin content could increase resistance to stresses. Here, we tested an engineering strategy to increase thiamin content in Arabidopsis. Thiamin is composed of a thiazole ring linked to a pyrimidine ring by a methylene bridge. THI1 and THIC are the first committed steps in the synthesis of the thiazole and pyrimidine moieties, respectively. Arabidopsis plants were transformed with a vector containing the THI1-coding sequence under the control of a constitutive promoter. Total thiamin leaf content in THI1 plants was up approximately 2-fold compared with the wild type. THI1-overexpressing lines were then crossed with pre-existing THIC-overexpressing lines. Resulting THI1 × THIC plants accumulated up to 3.4- and 2.6-fold more total thiamin than wild-type plants in leaf and seeds, respectively. After inoculation with Pseudomonas syringae, THI1 × THIC plants had lower populations than the wild-type control. However, THI1 × THIC plants subjected to various abiotic stresses did not show any visible or biochemical changes compared with the wild type. We discuss the impact of engineering thiamin biosynthesis on the nutritional value of plants and their resistance to biotic and abiotic stresses.
Wei Dong; Virginia O. Stockwell; Aymeric Goyer. Enhancement of Thiamin Content inArabidopsis thalianaby Metabolic Engineering. Plant and Cell Physiology 2015, 56, 2285 -2296.
AMA StyleWei Dong, Virginia O. Stockwell, Aymeric Goyer. Enhancement of Thiamin Content inArabidopsis thalianaby Metabolic Engineering. Plant and Cell Physiology. 2015; 56 (12):2285-2296.
Chicago/Turabian StyleWei Dong; Virginia O. Stockwell; Aymeric Goyer. 2015. "Enhancement of Thiamin Content inArabidopsis thalianaby Metabolic Engineering." Plant and Cell Physiology 56, no. 12: 2285-2296.
Potato virus Y (PVY) is one of the most important plant viruses affecting potato production. The interactions between potato and PVY are complex and the outcome of the interactions depends on the potato genotype, the PVY strain, and the environmental conditions. A potato cultivar can induce resistance to a specific PVY strain, yet be susceptible to another. How a single potato cultivar responds to PVY in both compatible and incompatible interactions is not clear. In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare the transcriptional changes in leaves of potato upon inoculation with PVY. We used two potato varieties: Premier Russet, which is resistant to the PVY strain O (PVYO) but susceptible to the strain NTN (PVYNTN), and Russet Burbank, which is susceptible to all PVY strains that have been tested. Leaves were inoculated with PVYO or PVYNTN, and samples were collected 4 and 10 h post inoculation (hpi). A larger number of differentially expressed (DE) genes were found in the compatible reactions compared to the incompatible reaction. For all treatments, the majority of DE genes were down-regulated at 4 hpi and up-regulated at 10 hpi. Gene Ontology enrichment analysis showed enrichment of the biological process GO term “Photosynthesis, light harvesting” specifically in PVYO-inoculated Premier Russet leaves, while the GO term “nucleosome assembly” was largely overrepresented in PVYNTN-inoculated Premier Russet leaves and PVYO-inoculated Russet Burbank leaves but not in PVYO-inoculated Premier Russet leaves. Fewer genes were DE over 4-fold in the incompatible reaction compared to the compatible reactions. Amongst these, five genes were DE only in PVYO-inoculated Premier Russet leaves, and all five were down-regulated. These genes are predicted to encode for a putative ABC transporter, a MYC2 transcription factor, a VQ-motif containing protein, a non-specific lipid-transfer protein, and a xyloglucan endotransglucosylase-hydroxylase. Our results show that the incompatible and compatible reactions in Premier Russet shared more similarities, in particular during the initial response, than the compatible reactions in the two different hosts. Our results identify potential key processes and genes that determine the fate of the reaction, compatible or incompatible, between PVY and its host.
Aymeric Goyer; Launa Hamlin; James M. Crosslin; Alex Buchanan; Jeff H. Chang. RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection. BMC Genomics 2015, 16, 1 -13.
AMA StyleAymeric Goyer, Launa Hamlin, James M. Crosslin, Alex Buchanan, Jeff H. Chang. RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection. BMC Genomics. 2015; 16 (1):1-13.
Chicago/Turabian StyleAymeric Goyer; Launa Hamlin; James M. Crosslin; Alex Buchanan; Jeff H. Chang. 2015. "RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection." BMC Genomics 16, no. 1: 1-13.
Thiamin can break down to its thiazole and pyrimidine moieties, which plants and microbes salvage for re-use in thiamin synthesis. We have biochemically and genetically characterized a plant enzyme for pyrimidine moiety salvage; this enzyme has microbial homologues.
Remi Zallot; Mohammad Yazdani; Aymeric Goyer; Michael J. Ziemak; Jiahn-Chou Guan; Donald R. Mccarty; Valérie De Crécy-Lagard; Svetlana Gerdes; Timothy J. Garrett; Jordi Benach; John F. Hunt; David. K. Shintani; Andrew D. Hanson. Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine. Biochemical Journal 2014, 463, 145 -155.
AMA StyleRemi Zallot, Mohammad Yazdani, Aymeric Goyer, Michael J. Ziemak, Jiahn-Chou Guan, Donald R. Mccarty, Valérie De Crécy-Lagard, Svetlana Gerdes, Timothy J. Garrett, Jordi Benach, John F. Hunt, David. K. Shintani, Andrew D. Hanson. Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine. Biochemical Journal. 2014; 463 (1):145-155.
Chicago/Turabian StyleRemi Zallot; Mohammad Yazdani; Aymeric Goyer; Michael J. Ziemak; Jiahn-Chou Guan; Donald R. Mccarty; Valérie De Crécy-Lagard; Svetlana Gerdes; Timothy J. Garrett; Jordi Benach; John F. Hunt; David. K. Shintani; Andrew D. Hanson. 2014. "Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine." Biochemical Journal 463, no. 1: 145-155.
Genes specifying the thiamin monophosphate phosphatase and adenylated thiazole diphosphatase steps in fungal and plant thiamin biosynthesis remain unknown, as do genes for ThDP (thiamin diphosphate) hydrolysis in thiamin metabolism. A distinctive Nudix domain fused to Tnr3 (thiamin diphosphokinase) in Schizosaccharomyces pombe was evaluated as a candidate for these functions. Comparative genomic analysis predicted a role in thiamin metabolism, not biosynthesis, because free-standing homologues of this Nudix domain occur not only in fungi and plants, but also in proteobacteria (whose thiamin biosynthesis pathway has no adenylated thiazole or thiamin monophosphate hydrolysis steps) and animals (which do not make thiamin). Supporting this prediction, recombinant Tnr3 and its Saccharomyces cerevisiae, Arabidopsis and maize Nudix homologues lacked thiamin monophosphate phosphatase activity, but were active against ThDP, and up to 60-fold more active against diphosphates of the toxic thiamin degradation products oxy- and oxo-thiamin. Deleting the S. cerevisiae Nudix gene (YJR142W) lowered oxythiamin resistance, overexpressing it raised resistance, and expressing its plant or bacterial counterparts restored resistance to the YJR142W deletant. By converting the diphosphates of damaged forms of thiamin into monophosphates, the Tnr3 Nudix domain and its homologues can pre-empt the misincorporation of damaged diphosphates into ThDP-dependent enzymes, and the resulting toxicity.
Aymeric Goyer; Ghulam Hasnain; Océane Frelin; Maria A. Ralat; Jesse F. Gregory; Andrew D. Hanson. A cross-kingdom Nudix enzyme that pre-empts damage in thiamin metabolism. Biochemical Journal 2013, 454, 533 -542.
AMA StyleAymeric Goyer, Ghulam Hasnain, Océane Frelin, Maria A. Ralat, Jesse F. Gregory, Andrew D. Hanson. A cross-kingdom Nudix enzyme that pre-empts damage in thiamin metabolism. Biochemical Journal. 2013; 454 (3):533-542.
Chicago/Turabian StyleAymeric Goyer; Ghulam Hasnain; Océane Frelin; Maria A. Ralat; Jesse F. Gregory; Andrew D. Hanson. 2013. "A cross-kingdom Nudix enzyme that pre-empts damage in thiamin metabolism." Biochemical Journal 454, no. 3: 533-542.
Biofortification of staple crops like potato via breeding is an attractive strategy to reduce human micronutrient deficiencies. A prerequisite is metabolic phenotyping of genetically diverse material which can potentially be used as parents in breeding programs. Thus, the natural genetic diversity of thiamin and folate contents was investigated in indigenous cultivated potatoes (Solanum tuberosum group Andigenum) and wild potato species (Solanum section Petota). Significant differences were found among clones and species. For about 50% of the clones there were variations in thiamin and folate contents between years. Genotypes which contained over 2-fold the thiamin and 4-fold the folate content compared to the modern variety Russet Burbank were identified and should be useful material to integrate in breeding programs which aim to enhance the nutritional value of potato. Primitive cultivars and wild species with widely different amounts of thiamin and folate will also be valuable tools to explore their respective metabolic regulation.
Aymeric Goyer; Kortney Sweek. Genetic Diversity of Thiamin and Folate in Primitive Cultivated and Wild Potato (Solanum) Species. Journal of Agricultural and Food Chemistry 2011, 59, 13072 -13080.
AMA StyleAymeric Goyer, Kortney Sweek. Genetic Diversity of Thiamin and Folate in Primitive Cultivated and Wild Potato (Solanum) Species. Journal of Agricultural and Food Chemistry. 2011; 59 (24):13072-13080.
Chicago/Turabian StyleAymeric Goyer; Kortney Sweek. 2011. "Genetic Diversity of Thiamin and Folate in Primitive Cultivated and Wild Potato (Solanum) Species." Journal of Agricultural and Food Chemistry 59, no. 24: 13072-13080.
Thiamine pyrophosphate (vitamin B1) is an essential nutrient in the human diet, and is often referred as the energy vitamin. Potato contains modest amounts of thiamine. However, the genetic variation of thiamine concentrations in potato has never been investigated. In this study, we determined thiamine concentrations in freshly-harvested unpeeled tubers of 54 potato clones, the majority of them originating from the Pacific Northwest Potato Development Program. Tubers from 39 clones were collected from four different environmental conditions. Thiamine concentrations ranged from 292 to 1,317 ng g−1 fresh weight, which gives a good estimate of the genetic variation available in Solanum tuberosum ssp. tuberosum. Thirteen clones/varieties contained >685 ng g−1 fresh weight and four had >800 ng g−1 fresh weight over multiple harvests, indicating that these genotypes would contribute a significant amount of thiamine in the diet (>10% of the Recommended Daily Allowance based on a 175- or 150-g serving, respectively). Broad-sense heritability for thiamine content was calculated as 0.49 with a 95% confidence interval of 0.21–0.72, suggesting that genetic variation accounted for about 50% of the observed variation. There were significant clone and clone x environment effects. After accounting for environmental variation, 25 clones were unstable across environments. Tubers harvested at a mature stage late in the growing season had higher amounts of thiamine than tubers harvested at a young stage early in the season. Storage at cold temperature did not lead to significant thiamine loss; instead, thiamine concentrations slightly increased during storage in some genotypes. These results suggest that increasing the concentration of thiamine in potato is feasible and that all potato varieties may one day be a significant source of thiamine in the human diet. La tiamina-pirofosfato (vitamin B1) es un nutriente esencial en la dieta humana, y a menudo se refiere a ella como la vitamina de la energía. La papa contiene cantidades modestas de tiamina. No obstante, la variación genética de las concentraciones de tiamina en papa nunca se ha investigado. En este estudio determinamos las concentraciones de tiamina en tubérculos sin pelar recién cosechados de cincuenta y cuatro clones de papa, la mayoría de ellos se originaron del Programa de Desarrollo de Papa del Pacífico-Noroeste. Se colectaron tubérculos de treinta y nueve clones de cuatro diferentes condiciones ambientales. Las concentraciones de tiamina variaron de292 a 1,317 ng g-1 de peso fresco, lo que da una buena estimación de la variación genética disponible en Solanum tuberosum ssp. Tuberosum. Trece clones/variedades contenían >685 ng g-1 de peso fresco y cuatro tuvieron >800 ng g-1 de peso fresco en múltiples cosechas, indicando que estos genotipos contribuirían con una cantidad significativa de tiamina en la dieta (>10% de la cantidad recomendada diariamente basada en un 175 o 150 g por porción, respectivamente). Se calculó la heredabilidad en amplio sentido para el contenido de tiamina como 0.49 con un intervalo de confianza de 95% de 0.21–0.72, lo que sugiere que la variación genética contó para cerca de 50% de la variación observada. Hubo efectos significativos de clon y de clon x ambiente. Después de contabilizar para la variación por el ambiente, 25 clones fueron inestables entre ambientes. Los tubérculos cosechados en un estado maduro tarde en el ciclo de cultivo tuvieron cantidades más altas de tiamina que los tubérculos cosechados en un estado joven temprano en el ciclo. El almacenamiento a baja temperatura no condujo a pérdida significativa de tiamina; más bien, las concentraciones de tiamina aumentaron ligeramente durante el almacenamiento en algunos genotipos. Estos resultados sugieren que es posible el aumento en la concentración de tiamina y que todas las variedades de papa pudieran algún día ser una fuente significativa de tiamina en la dieta humana.
Aymeric Goyer; Kathleen G. Haynes. Vitamin B1 Content in Potato: Effect of Genotype, Tuber Enlargement, and Storage, and Estimation of Stability and Broad-Sense Heritability. American Potato Journal 2011, 88, 374 -385.
AMA StyleAymeric Goyer, Kathleen G. Haynes. Vitamin B1 Content in Potato: Effect of Genotype, Tuber Enlargement, and Storage, and Estimation of Stability and Broad-Sense Heritability. American Potato Journal. 2011; 88 (4):374-385.
Chicago/Turabian StyleAymeric Goyer; Kathleen G. Haynes. 2011. "Vitamin B1 Content in Potato: Effect of Genotype, Tuber Enlargement, and Storage, and Estimation of Stability and Broad-Sense Heritability." American Potato Journal 88, no. 4: 374-385.