The environmental education and the promotion of the sustainability are two of the main axes in the international guidelines around the world. Education institutions must play an active role to achieve environment and sustainability awareness, and government educational policies should provide education institutions the necessary tools. This study tries to analyze the way in which these topics have been integrated in the Spanish compulsory curriculum through the normative review. Specifically, a content analysis has been used to quantify environmental and sustainability references, both in the subjects they integrate and the contents and standards which they relate with. The results show that: (1) there is not a transversal consideration of these terms; (2) the integration is greater in the Secondary Education; (3) environmental and sustainability treatment is mostly theoretical and focused on instrumental subjects; (4) when the approach is practical, it is often linked to reflections processes. Thus, we conclude on the need for governmental institutions to increase the presence of these terms in the educational curriculum and to integrate them in a transversal way and, through specific content, skills that contribute to build a collective social awareness of the importance of these phenomena in our society, including them as content as well as in the initial and continuous training.

The aim of this preliminary study was to detect cytological changes in the oral mucosa after using a mouth wash with alcohol. A prospective double-blind, controlled study was performed, for 6 months. Group 1 consisted of 30 subjects who used a mouth rinse with 26.9% of alcohol [Listerine] and Group 2 consisted of 30 subjects who used a mouth rinse with the same ingredients but with no alcohol. We obtained three cytological samples from the oral mucosa. The presence of cytological atypia, binucleation and karyorrhesis, and type of cells were studied. We also used a fluorescent in situ hybridization technique (FISH) in 15 samples in each group, for the micronucleus. We found no clinical mucosal alteration after using the mouth wash at the end of the study in either group. We observed no cytological differences between the groups at the end of the study (p>0.05). Regarding the study of the micronucleus by FISH, we observed no significant difference between the groups (p>0.05). Our results showed no cytological alteration in patients using a mouth rinse with alcohol, but these findings should be considered preliminary results, to be confirmed in a greater sample of patients.