This study assessed the floristic composition, biological spectrum, and phytogeographical elements of the Bin Dara western boundary of Pakistan. The flora consisted of 140 plant species belonging to 47 families. The most dominant family was Poaceae (with 14 species), followed by Asteraceae (11 species). The biological spectrum showed the therophytes (71 species, 50.71%) as the dominant life form class, followed by mega-phanerophytes (27 species, 19.28%), nano-phanerophytes (15 species, 10.71%), geophytes (10 species, 7.14%), chamaephytes (9 species, 6.42%), and hemi-cryptophytes (8 species, 5.71%). Leaf size classes comprised of nanophylls (48 species, 34.28%), microphylls (32 species, 22.85%), mesophylls (22 species, 15.71%), macrophylls (17 species, 12.14%), leptophylls (12 species, 8.57), megaphylls (8 species, 5.75%), and aphyllous (1 species, 0.71%). Cosmopolitan was the most prominent form of phytogeographic elements (with 17 species, 12.14%), followed by pantropical and Euro-Siberian Mediterranean Irano-Turanian (14 species, 10%) each. The pluriregional represented (13 species, 9.28%), Holarctic and Mediterranean Irano-Turanian (10 species, 7.14%), Irano-Turanian and Western Himalayan (9 species, 6.42%) each. It is recommended that further study is needed to map the vegetation, its indicators, and rare species that face a huge threat of endangerment.

The comparative in vitro antimicrobial activity of ethanol and chloroform leaves extracts from Acacia nilotica L. was studied. Leaves extracts exhibited considerable bacteriostatic activity against two Gram-positive and three Gram-negative strains. The antimicrobial action was compared with the effect of doxycycline antibiotic. The maximum zone of inhibition of 29 mm diameter was observed in Escherichia coli with ethanol extract while a minimum 8 mm zone of inhibition was found in Bacillus subtilis. Klebsiella pneumoniae showed marked resistance towards both ethanol and chloroform extracts. Among the fungal strains tested, Aspergillus flavus exhibited maximum sensitivity action of the extracts. This analysis revealed the high antimicrobial activity in the ethanol extract of A. nilotica L. than chloroform extract. It is recommended to isolate, identify and integrate the bioactive principle in these pathogens management. Key words: Acacia nilotica L., ethanol, extract, chloroform, pathogenic, minimum inhibitory concentration (MIC).

Germin-like proteins (GLPs) play an important role against various stresses. Vitis vinifera L. genome contains 7 GLPs; many of them are functionally unexplored. However, the computational analysis may provide important new insight into their function. Currently, physicochemical properties, subcellular localization, domain architectures, 3D structures, N-glycosylation & phosphorylation sites, and phylogeney of the VvGLPs were investigated using the latest computational tools. Their functions were predicted using the Search tool for the retrieval of interacting genes/proteins (STRING) and Blast2Go servers. Most of the VvGLPs were extracellular (43%) in nature but also showed periplasmic (29%), plasma membrane (14%), and mitochondrial- or chloroplast-specific (14%) expression. The functional analysis predicted unique enzymatic activities for these proteins including terpene synthase, isoprenoid synthase, lipoxygenase, phosphate permease, receptor kinase, and hydrolases generally mediated by Mn⁺ cation. VvGLPs showed similarity in the overall structure, shape, and position of the cupin domain. Functionally, VvGLPs control and regulate the production of secondary metabolites to cope with various stresses. Phylogenetically VvGLP1, -3, -4, -5, and VvGLP7 showed greater similarity due to duplication while VvGLP2 and VvGLP6 revealed a distant relationship. Promoter analysis revealed the presence of diverse cis-regulatory elements among which CAAT box, MYB, MYC, unnamed-4 were common to all of them. The analysis will help to utilize VvGLPs and their promoters in future food programs by developing resistant cultivars against various biotic (Erysiphe necator and in Powdery Mildew etc.) and abiotic (Salt, drought, heat, dehydration, etc.) stresses.