Peach production in Georgia, USA, extends from mid-May to mid-August. Multiple cultivars are commercially grown in the U.S., and each cultivar has unique fruit quality characteristics, which could influence consumer perception and acceptability. Among those, peach flavor has been minimally characterized among cultivars. Headspace-solid phase microextraction (HS–SPME) combined with gas chromatography–mass spectrometry (GC–MS) was used to characterize the volatile profile of 42 peach cultivars commercially grown in 2016. The goal of this research was to understand the aroma composition and content of peach cultivars grown in Georgia, USA. Thirty-six volatile compounds were identified. Significant differences (p ≤ 0.0001) were observed for all the flavor volatiles across all cultivars being evaluated. Esters were the major compounds isolated from the 42 peach cultivars, followed by lactones, alcohols, aldehydes, and terpenoids, in that order. Lactones, benzaldehyde, and linalool, which are known to be peach aroma compounds, exhibited the highest mean concentrations among the cultivars with ɣ- and δ-decalactones having a concentration of approximately 180 ng·g⁻¹ and 60 ng·g⁻¹, respectively. Among the cultivars, “Majestic” had the highest mean concentration of ɣ-decalactone, followed by “Southern Pearl” with approximately 503 ng·g⁻¹ and 443 ng·g⁻¹, respectively. “Southern Pearl” and “Fairtime” also exhibited the highest average concentration of δ-decalactone. The results obtained will help provide an understanding of the distribution of the various volatile organic compounds found in Georgia-grown peach cultivars.

Partial to complete self-incompatibility is normal in most Vaccinium L. (Ericaceae) species. Wild blueberry plants of several Florida provenances and species were self- and cross-pollinated in a greenhouse free of pollinators. Fruit set of V. darrowii Camp (2x), V. corymbosum L. (4x), V. arboreum Marsh (2x), and F ₁ ( V. darrowii × V. corymbosum ) hybrids was higher after cross-pollination than after self-pollination. Partial to complete self-incompatibility was present in V. darrowii , V. corymbosum , and their tetraploid F ₁ hybrids. The three V. arboreum clones tested were fully self-incompatible. Intra- and interpopulation crosses in V. corymbosum , V. darrowii , and V. darrowii × V. corymbosum hybrids were highly successful, and self-pollination reduced all fertility parameters. Advanced selections of V. corymbosum were the most self-compatible clones tested, possibly because self-compatibility has been increased by breeders selecting for reliable fruit set in large fields planted with one or a few clones. One southern highbush selection and two F ₁ hybrids had fruit set of more than 70% after self-pollination. These plants could be potentially used to breed plants that could be planted in single blocks providing reliable yield.

Fruit size is a highly valued commercial trait in peach. Competition among fruit and among other sinks on a tree reduces potential growth rate of the fruit. Hence, crop-load management strategies such as thinning (removal of flowers or fruit) are often practiced by growers to optimize fruit size. Thinning can be performed at bloom or during early fruit development and at different intensities to optimize fruit growth responses. Responses to thinning may be cultivar and location specific. The objective of the current study was to fine-tune thinning strategies in the southeastern United States, a major peach producing region. Timing and intensity of thinning were evaluated across multiple cultivars over three years. Thinning at bloom or at 21 d after full bloom (DAFB) improved fruit size in comparison to unthinned trees in ‘Cary Mac’ and ‘July Prince’, respectively, in one year. Bloom-thinning reduced fruit yield (kg per tree) in the above cultivars in one year, suggesting that flower thinning alone may not be a viable option in this region. Intensity of thinning, evaluated as spacings of 15 cm and 20 cm between fruit, did not differentially affect fruit weight or yield. However, fruit diameter decreased quadratically with increasing fruit number per tree in ‘Cary Mac’, ‘July Prince’ and ‘Summer Flame’. Similarly, fruit weight decreased quadratically in response to increase in fruit number per tree in ‘Cary Mac’ and ‘July Prince’. Further, yield-per-tree decreased with increasing fruit size in ‘Cary Mac’ and ‘July Prince’. Importantly, these relationships were cultivar specific. Together, the data suggest that achieving a target fruit number per tree is an effective strategy for crop-load management to optimize fruit size in southeastern peach production. The target fruit number per tree may potentially be achieved through a combination of flower and fruit-thinning during early fruit development. Such an approach may provide flexibility in crop-load management in relation to adverse weather events.

Phytophthora capsici causes seedling death, crown and root rot, fruit rot, and foliar blight on squash and pumpkins ( Cucurbita spp. L.). A total of 119 C. moschata accessions, from 39 geographic locations throughout the world, and a highly susceptible butternut squash cultivar, Butterbush, were inoculated with a suspension of three highly virulent P. capsici isolates from Florida to identify resistance to crown rot. Mean disease rating (DR) of the C. moschata collection ranged from 1.4 to 5 (0 to 5 scale with 0 resistant and 5 susceptible). Potential resistant and tolerant individuals were identified in the C. moschata collection. A set of 18 PIs from the original screen were rescreened for crown rot resistance. This rescreen produced similar results as the original screen ( r = 0.55, P = 0.01). The accessions PI 176531, PI 458740, PI 442266, PI 442262, and PI 634693 were identified with lowest rates of crown infection with a mean DR less than 1.0 and/or individuals with DR = 0. Further selections from these accessions could be made to develop Cucurbita breeding lines and cultivars with resistance to crown rot caused by P. capsici .

In Vaccinium L., most tetraploid hybrids between tetraploid cultivated highbush blueberry ( V. corymbosum L.) and diploid darrow's evergreen blueberry ( V. darrowii Camp) have been produced by exploiting V. darrowii 's tendency to produce 2n gametes and a strong triploid block, which greatly reduces the number of triploid hybrids produced. Colchicine-derived tetraploids offer an alternative method of producing V. darrowii plants that will easily cross with tetraploid Vaccinium species. V. darrowii (2n = 2x = 24) seeds were imbibed in 0.2% aqueous colchicine solution for 24 h. The seeds were germinated and seedlings whose morphology suggested colchicine effects were selected at various stages of development. No macromorphological changes were consistently associated with chromosome doubling. However, stomatal guard cells and pollen size increased substantially as a result of chromosome doubling. Several types of plants were identified after colchicine treatment: 1) plants with a doubled LI (epidermal tissues) and LII (internal tissues) plant layers; 2) periclinal chimeras with a doubled LI layer and a normal LII; and 3) periclinal chimeras with a doubled LII layer and a normal LI. Of ≈4000 seedlings that emerged from colchicine-treated seeds, 200 were selected for further examination based on leaf and stem morphology. Of the 200, five appeared to be tetraploid in LI, LII, or both layers based on stomatal guard cell size and pollen size. Crosses between colchicine-derived V. darrowii (4x) plants and southern highbush blueberry ( V. corymbosum ) cultivars (4x) were successful compared with 4x–2x and 2x–4x crosses using diploid V. darrowii and tetraploid southern highbush blueberry. Stomatal guard cells and pollen screening of the colchicine-treated plants were used as indicators of doubled V. darrowii plants and periclinal chimeras. The results from the crosses between colchicine-treated V. darrowii plants and tetraploid highbush blueberry cultivars confirmed the information obtained by stomata and pollen screening.