John W. Doyle

Dr. John W. Doyle


Research Focus & Metrics

Academic Career Length

10Years (2017-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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14 April 2021 in Agronomy
Verified
Journal Article
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JDJohn W. Doyle
John W. Doyle
SNSavithri Nambeesan
Savithri Nambeesan
AMAnish Malladi
Anish Malladi
Sustaining the fourfold increase in blueberry (Vaccinium sp.) production witnessed during the previous two decades requires better understanding of its mineral nutrient physiology. The primary goals of this review are to evaluate our current understanding of the physiology of nitrogen (N) and calcium (Ca) nutrition in blueberry. Nitrogen concentration in blueberry ranges from 0.4% to >2% across organs. Blueberry uses N in various forms (organic and inorganic), but it appears to display preference for ammonium (NH4+) over nitrate (NO3−). The roles of N acquisition, translocation and assimilation in determining N-source preference in blueberry are evaluated. Calcium plays important roles in determining fruit quality owing to its function in maintaining cell wall and membrane integrity. It is unique in its translocation characteristics being transported primarily via the xylem. Fruit [Ca2+] typically declines from around 0.2% during early development to <0.05% at ripening. Modes of Ca acquisition and transport to the fruit, and various approaches to improve fruit [Ca2+] are discussed. Areas where further research is warranted to improve our understanding of N and Ca physiology in blueberry are identified. Such knowledge is essential for sustainable nutrient management, improving productivity, and enhancing fruit quality in blueberry.

1 September 2018 in Horticulturae
Verified
Journal Article
Open access
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YWYi-Wen Wang
Yi-Wen Wang
AMAnish Malladi
Anish Malladi
JDJohn W. Doyle
John W. Doyle
Harald SchermHarald Scherm
Harald Scherm
SNSavithri Nambeesan
Savithri Nambeesan
Ripening in blueberry fruit is irregular and occurs over an extended period requiring multiple harvests, thereby increasing the cost of production. Several phytohormones contribute to the regulation of fruit ripening. Certain plant growth regulators (PGRs) can alter the content, perception, or action of these phytohormones, potentially accelerating fruit ripening and concentrating the ripening period. The effects of three such PGRs—ethephon, abscisic acid, and methyl jasmonate—on fruit ripening were evaluated in the rabbiteye blueberry (Vaccinium virgatum) cultivars ‘Premier’ and ‘Powderblue’. Application of ethephon, an ethylene-releasing PGR, at 250 mg L−1 when 30–40% of fruit on the plant were ripe, accelerated ripening by increasing the proportion of blue (ripe) fruit by 1.5–1.8-fold within 4 to 7 days after treatment in both cultivars. Ethephon applications did not generally alter fruit quality characteristics at harvest or during postharvest storage, except for a slight decrease in juice pH at 1 day of postharvest storage and an increase in fruit firmness and titratable acidity after 15 days of postharvest storage in Powderblue. In Premier, ethephon applications decreased the proportion of defective fruit at 29 days of postharvest storage. Abscisic acid (600–1000 mg L−1) and methyl jasmonate (0.5–1 mM) applications did not alter the proportion of ripe fruit in either cultivar. These applications also had little effect on fruit quality characteristics at harvest and during postharvest storage. None of the above PGR applications affected the development of naturally occurring postharvest pathogens during storage. Together, data from this study indicated that ethephon has the potential to accelerate ripening in rabbiteye blueberry fruit, allowing for a potential decrease in the number of fruit harvests.

22 July 2020 in Horticulturae
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Journal Article
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MSMary Sutton
Mary Sutton
JDJohn W. Doyle
John W. Doyle
Dario ChavezDario Chavez
Dario Chavez
AMAnish Malladi
Anish Malladi
Fruit size is a highly valued commercial trait in peach. Competition among fruit and among other sinks on a tree reduces potential growth rate of the fruit. Hence, crop-load management strategies such as thinning (removal of flowers or fruit) are often practiced by growers to optimize fruit size. Thinning can be performed at bloom or during early fruit development and at different intensities to optimize fruit growth responses. Responses to thinning may be cultivar and location specific. The objective of the current study was to fine-tune thinning strategies in the southeastern United States, a major peach producing region. Timing and intensity of thinning were evaluated across multiple cultivars over three years. Thinning at bloom or at 21 d after full bloom (DAFB) improved fruit size in comparison to unthinned trees in ‘Cary Mac’ and ‘July Prince’, respectively, in one year. Bloom-thinning reduced fruit yield (kg per tree) in the above cultivars in one year, suggesting that flower thinning alone may not be a viable option in this region. Intensity of thinning, evaluated as spacings of 15 cm and 20 cm between fruit, did not differentially affect fruit weight or yield. However, fruit diameter decreased quadratically with increasing fruit number per tree in ‘Cary Mac’, ‘July Prince’ and ‘Summer Flame’. Similarly, fruit weight decreased quadratically in response to increase in fruit number per tree in ‘Cary Mac’ and ‘July Prince’. Further, yield-per-tree decreased with increasing fruit size in ‘Cary Mac’ and ‘July Prince’. Importantly, these relationships were cultivar specific. Together, the data suggest that achieving a target fruit number per tree is an effective strategy for crop-load management to optimize fruit size in southeastern peach production. The target fruit number per tree may potentially be achieved through a combination of flower and fruit-thinning during early fruit development. Such an approach may provide flexibility in crop-load management in relation to adverse weather events.

5 September 2018 in Horticulturae
Verified
Journal Article
Open access
PDF file
SNSavithri Nambeesan
Savithri Nambeesan
JDJohn W. Doyle
John W. Doyle
HCHelaina Capps
Helaina Capps
CSChip Starns
Chip Starns
Harald SchermHarald Scherm
Harald Scherm
With the growing popularity of blueberries and the associated increase in blueberry imports and exports worldwide, delivering fruit with high quality, longer shelf-life, and meeting phytosanitary requirements has become increasingly important. The objective of this study was to determine the effects of electron beam irradiation using a new Electronic Cold-PasteurizationTM (ECPTM) technology on fruit quality, microbial safety, and postharvest disease development in two southern highbush blueberry cultivars, ‘Farthing’ and ‘Rebel’. Fruit packed in clamshells were subjected to four levels of ECPTM irradiation (0, 0.15, 0.5, and 1.0 kGy) and evaluated for fruit quality attributes, surface microbial load, and postharvest disease incidence during various storage times after treatment and cold storage. Overall, there was no effect of irradiation on visual fruit quality in either cultivar. Fruit firmness and skin toughness in ‘Farthing’ was reduced following irradiation at 1.0 kGy, but no such effect was observed in ‘Rebel’. Other fruit quality characteristics such as fruit weight, total soluble solids content, or titratable acidity were not affected. Irradiation at 1.0 kGy significantly reduced total aerobic bacteria and yeast on the fruit surface, and in the case of ‘Rebel’, also levels of total coliform bacteria. There was no significant effect of irradiation on postharvest disease incidence in these trials. Overall, data from this study suggests that an irradiation dose lower than 1.0 kGy using ECPTM can be useful for phytosanitary treatment in blueberry fruit while avoiding undesirable effects on fruit quality in a cultivar-dependent manner.