A study was conducted to identify the cytochrome P450 (CYP, CYP450) enzyme orthologs involved in the bioactivation of aflatoxin B1 (AFB1) into the hi

The role of different cytochrome P450 enzymes on the metabolism of 3-methylindole (3MI) was investigated using selective chemical inhibitors. Eight chemical inhibitors of P450 enzymes were screened for their inhibitory specificity towards 3MI metabolism in porcine microsomes: alpha-naphthoflavone (CYP1A1/2), 8-methoxypsoralen (CYP2A6), menthofuran (CYP2A6), diethyldithiocarbamate (CYP2A6), 4-methylpyrazole (CYP2E1), sulphaphenazole (CYP2C9), quinidine (CYP2D6), and troleandomycin (CYP3A4). The production of 3MI metabolites was only affected by the presence of inhibitors of CYP2A6 and CYP2E1 in the microsomal incubations. In a second experiment, a set of porcine microsomes (n = 30) was analyzed for CYP2A6 content by protein immunoblot analysis and for their coumarin 7-hydroxylation activity (CYP2A6 activity). Both CYP2A6 content and enzymatic activity were found to be highly and negatively correlated with 3MI fat content. The results of the present study indicate that the CYP2A6 porcine ortholog plays an important role in the metabolism of 3MI and that measurement of CYP2A6 levels and/or activity could be a useful marker for 3MI-induced boar taint.

A method has been developed for the simultaneous analysis of 22 mycotoxins in wheat, barley, oats, rye and maize grain. Analysis is carried out with liquid chromatography-electrospray ionisation tandem mass spectrometry. The compounds included in this analysis are aflatoxins, sterigmatocystin, cyclopiazonic acid, tricothecenes, ochratoxin A, fumonisins, zearalonone, and ergot alkaloids. Sample extraction (2 g) with acetonitrile:water (8 ml, 80:20) was carried out for 2 min using a commercial sample preparation apparatus (Stomacher®). The extract was then centrifuged, filtered and analysed. Extraction of fumonisins from maize (2 g) was optimised by first extracting the maize with acetonitrile: water (5 ml, 80:20) followed by the addition of water (3 ml), which permitted extraction of the 22 mycotoxins, including the fumonisins. Chromatography was carried out with a minicolumn (7.5×2.1 mm, 5 µm) (5 µl sample injection) and in 11 min, including column reconditioning. Analysis was carried out with 2 MRM transitions for the precursor ions. All method detection limits were below current maximum Canadian residue limits. Matrix effects for each compound in each of the 5 matrices were estimated and ranged from 70 to 149%, but most were 100±10%. Accuracy, repeatability and ruggedness were established. Proficiency samples from FERA (Food and Environment Research Agency, Sand Hutton, York, UK) were tested and are reported. Finally, 100 field samples of the various grains were tested with this method and are reported with the observation of numerous mycotoxins in all matrices, including ergotamine in winter wheat.

1. This research evaluated differences in hepatic in vitro metabolism of aflatoxin B1 (AFB1) on selected avian species. 2. Microsomal and cytosolic liver fractions were obtained from chickens, duck...

Poultry has commonly been considered highly susceptible to aflatoxins. However, among domestic fowl there is wide variability in specific species sen

Due to its tropical location, chains of mountains, inter-Andean valleys, Amazon basin area, eastern plains and shores on both the Atlantic and Pacific Oceans, Colombia has many ecosystems and the second largest plant biodiversity in the world. Many plant species, both native and naturalized, are currently recognized as toxic for both animals and humans, and some of them are known to cause their toxic effects due to their alkaloid content. Among these, there are plants containing the hepatotoxic pyrrolizidine alkaloids, neurotoxins such as the indolizidine alkaloid swainsonine and the piperidine alkaloids coniine and γ-coniceine and tropane alkaloids. Unfortunately, the research in toxic plants in Colombia is not nearly proportional to its plant biodiversity and the scientific information available is only very scarce. The present review aims at summarizing the scarce information about plant alkaloid toxicosis in animals and humans in Colombia.