Plasma-activated water (PAW), generated through Surface Dielectric Barrier Discharge, was tested against microbial contaminants. We assessed how the time of exposure to plasma treatment and the gas flow rate impact the chemical composition of PAW, and, in turn, how it influences these factors influence its efficacy against microorganisms. The effectiveness of PAW treatments was evaluated against the fungal phytopathogen Botrytis cinerea and both pathogenic (Xanthomonas campestris pv. vesicatoria) and beneficial (Bacillus amyloliquefaciens) bacteria. The physicochemical properties of PAW were assessed as the concentration of reactive species, namely, NO₃⁻ and NO₂⁻ and H₂O₂, pH, and oxidation-reduction potential. Higher levels of reactive species and lower pH during longer treatments were associated with greater antimicrobial efficacy. A correlation study and Principal Component Analysis demonstrated that the RONS content in PAW affected antimicrobial activity, with stronger correlations between NO₂⁻, H₂O₂, and fungal inhibition, as well as between NO₂⁻ and bacterial inhibition. Almost complete inhibition was reached after 1 min of treatment for bacteria (log reduction of 4.5 for B. amyloliquefaciens and 5.1 for X. campestris) and after 3 min of treatment for B. cinerea (approximately 90% inhibition of conidial germination). The obtained results contribute to defining optimized treatment conditions using PAW for antimicrobial decontamination of plant products.

The use of resistance inducers is a novel strategy to elicit defence responses in strawberry fruit to protect against preharvest and postharvest decay. However, the mechanisms behind the specific resistance inducers are not completely understood. Here, global transcriptional changes in strawberry fruit were investigated using RNA-Seq technology. Preharvest, benzothiadiazole and chitosan were applied to the plant canopy, and the fruit were harvested at 6, 12 and 24 h post-treatment. Overall, 5,062 and 5,210 differentially expressed genes (fold change, ≥2) were identified in these fruit under the benzothiadiazole and chitosan treatments, respectively, as compared to the control expression. About 80% of these genes were differentially expressed by both elicitors. Comprehensive functional enrichment analysis highlighted different gene modulation over time for transcripts associated with photosynthesis and heat-shock proteins, according to elicitor. Up-regulation of genes associated with reprogramming of protein metabolism was observed in fruit treated with both elicitors, which led to increased storage proteins. Several genes associated with the plant immune system, hormone metabolism, systemic acquired resistance, and biotic and abiotic stresses were differentially expressed in treated versus untreated plants. The RNA-Seq output was confirmed using RT-qPCR for 12 selected genes. This study demonstrates that these two elicitors affect cell networks associated with plant defences in different ways, and suggests a role for chloroplasts as the primary target in this modulation of the plant defence responses, which actively communicate these signals through changes in redox status. The genes identified in this study represent markers to better elucidate plant/ pathogen/ resistance-inducer interactions, and to plan novel sustainable disease management strategies.