Cytoplasmic male sterility (CMS) provides an irreplaceable strategy for commercial exploitation of heterosis and producing high-yielding hybrid rice. The exogenous application of plant growth regulators could improve outcrossing rates of the CMS lines by affecting floral traits and accordingly increase hybrid rice seed production. The present study aimed at exploring the impact of growth regulators such as gibberellic acid (GA₃), indole-3-acetic acid (IAA), and naphthalene acetic acid (NAA) on promoting floral traits and outcrossing rates in diverse rice CMS lines and improving hybrid rice seed production. The impact of foliar applications of growth regulators comprising GA₃ at 300 g/ha or GA₃ at 150 g/ha + IAA at 50 g/ha + NAA at 200 g/ha versus untreated control was investigated on floral, growth, and yield traits of five diverse CMS lines. The exogenously sprayed growth regulators, in particular, the combination of GA₃, IAA, and NAA (T3) boosted all studied floral, growth, and yield traits in all tested CMS lines. Moreover, the evaluated CMS lines exhibited significant differences in all measured floral traits. L2, L3, and L1 displayed the uppermost spikelet opening angle, duration of spikelet opening, total stigma length, style length, stigma brush, and stigma width. In addition, these CMS lines exhibited the highest plant growth and yield traits, particularly under T3. Consequently, exogenous application of GA₃, IAA, and NAA could be exploited to improve the floral, growth, and yield traits of promising CMS lines such as L2, L3, and L1, hence increasing outcrossing rates and hybrid rice seed production.

The occurrence of anthocyanins in rice (Oryza sativa) and barley (Hordeum vulgare) varies among cultivars, with pigmented varieties (e.g., black rice and purple barley) accumulating higher concentrations due to genetic and environmental factors. The biosynthesis of anthocyanins is regulated by a complex network of structural and regulatory genes. Key enzymes in the pathway include chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), and UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT). These genes are tightly controlled by transcription factors (TFs) from the MYB, bHLH (basic helix–loop–helix), and WD40 repeat families, which form the MBW (MYB-bHLH-WD40) regulatory complex. In rice, OsMYB transcription factors such as OsMYB3, OsC1, and OsPL (Purple Leaf) interact with OsbHLH partners (e.g., OsB1, OsB2) to activate anthocyanin biosynthesis. Similarly, in barley, HvMYB genes (e.g., HvMYB10) coordinate with HvbHLH TFs to regulate pigment accumulation. Environmental cues, such as light, temperature, and nutrient availability, further modulate these TFs, influencing the production of anthocyanin. Understanding the genetic and molecular mechanisms behind the biosynthesis of anthocyanins in rice and barley provides opportunities for the development of biofortification strategies that enhance their nutritional value.

The nutritional and health-promoting properties of tomatoes (Solanum lycopersicum), a highly significant crop, are attributed to their abundance of beneficial components, such as flavonoids, phenolic compounds, and carotenoids (including lycopene and β-carotene). The occurrence of these bioactive molecules is influenced by genetic, environmental, and agronomic factors, with ripening playing a critical role in their accumulation. This abstract delves into the molecular machinery controlling phytochemical accumulation, with a specific focus on the regulation of lycopene biosynthesis. The RIPENING-INHIBITOR (RIN) transcription factor, a master regulator of fruit maturation, exerts direct control over lycopene accumulation by binding to the promoters of critical biosynthetic genes. RIN directly activates the expression of PHYTENE SYNTHASE 1 (PSY1), the key rate limiting enzyme committing metabolic flux to the carotenoid pathway, and PDS, encoding phytocene desaturase, thereby orchestrating the massive lycopene synthesis characteristic of the ripening transition. Strategies for the biofortification of tomato fruits have leveraged this understanding through targeted genetic manipulation. Overexpression of key enzymes, such as the bacterial CrtB (phytoene synthase) or manipulation of the endogenous PSY1, has successfully enhanced lycopene flux. More profoundly, the manipulation of transcription factors offers a powerful multi-gene approach. For instance, the overexpression of fruit-specific promoters driving RIN or other regulators like HYR (High Pigment) can simultaneously improve the entire pathway, leading to substantial increases in lycopene content. Flavonoids and phenolic compounds are produced by the phenylpropanoid pathway, which is regulated by enzymes such as chalcone synthase (CHS) and phenylalanine ammonialyase (PAL). Gene regulation of these pathways involves a complex interplay of transcription factors (e.g., RIN, NOR, and HY5) and phytohormones (e.g., ethylene and abscisic acid), which modulate expression patterns during fruit development and stress responses. Phytochemical levels are also significantly influenced by environmental factors; for instance, optimal lycopene synthesis occurs at 20-25 °C, while higher temperatures above 30 °C inhibit lycopene accumulation and promote beta-carotene synthesis, a shift mediated by the temperature-sensitive expression of key genes, including those regulated by RIN. Naturally occurring or induced mutations in genes such as DET1 and HP2, which are negative regulators of light signal transduction, result in high pigment phenotypes with dramatically increased lycopene and flavonoid content. The successful application of metabolic engineering and transcription factor manipulation for biofortification holds immense promises for developing next-generation tomato cultivars with amplified health-promoting properties, directly linking agricultural science to improved human health outcomes through the mitigation of chronic diseases like cancer and cardiovascular disorders.

Rice is the world’s largest food crop, and its production needs to be doubled by 2050 to cope with population growth and associated demand. In addition to the value of improving yields, quality is also important for breeders and consumers, but it pays less attention in arid regions. During two successive summer growing seasons, the experimental material focused on 34 genotypes developed from different crosses on Fn generation after fixation as well as six of the most recent commercial cultivars used for comparisons. The results showed that a high yield of grain followed by high milling and grain quality characteristics were observed among the 34 genotypes used in this analysis. Highly important and positive correlations between the percentage of hulling and the percentage of milling (0.424) and the yield ability could be accomplished by choosing the number of panicles per plant and the weight of the panicles. Selection criteria for good quality should be met by the percentage of head rice and many mineral elements, particularly zinc and iron. As a consequence, the genotypes M.J 5460S/SK105-1, M.J 5460S/GZ7768-1, M.J 5460S/G177-1, M.J 5460S/SK105-3 and M.J 5460S/SK106-4 had desirable high yield and quality characteristics and could be used as promising accessions to the rice breeding program in arid regions. In addition to commercial genotypes, improved Japonica rice genotypes could be produced in arid conditions for higher yield and quality, leading to an increase in total production, supporting food security and nutrition.

Rice is considered a strategic crop for many countries around the world, being the main cash crop for farmers. Water shortage stress occurrence as a result of climate change is among the main threats challenging rice breeders in the last few decades. In the current study, 19 Fn-lines were developed from four populations by crossing a reverse thermo-responsive genic male sterile (rTGMS) line, M.J.5460S, with the three high-quality Egyptian commercial cultivars Giza177, Sakha105, Sakha106 and the promising line GZ7768 as male parents. These newly developed lines, along with their parents, and two water shortage stress-tolerant international genotypes (Azucena and IRAT170), were cultivated under water-shortage stress conditions and compared with their performance under well-watered conditions. Results indicated that the yielding ability of the 19 newly developed lines exceeded those for the two Egyptian parents (Giza177 and Sakha105) under well-watered conditions. The lines M.J5460S/GIZA177-3 and M.J5460S/GIZA177-12 were the best performing genotypes under water shortage stress conditions. The genetic and heritability in broad sense estimates indicated that direct selection for grain yield (GY) under water-shortage stress is highly effective in the current study. Molecular marker analysis revealed that M.J5460S/GIZA177-3 had accumulated the quantitative trait loci (QTL)s, on the chromosomes 2, 3, and 9, which contribute to GY under water-shortage stress from their high yielding tolerant ancestor, M.J5460S. It could be concluded that those lines are high yielding under both well-watered and water-stress conditions harboring several QTLs for yield enhancement under both conditions and that the markers RM555, RM14551, RM3199, RM257, RM242, and RM410 are among the markers that could be used in marker-assisted selection (MAS) breeding programs for such stress condition.