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Background: Microalgal extracts are an important source of bioactive compounds with antioxidant and anti-inflammatory properties that can be used in cosmetics. The microalgae Phaeodactylum tricornutum (PT) is known for its high content of omega-3 fatty acids, which are known to attenuate inflammation. Here, we explore the effects of aqueous microencapsulated extract of PT on lipopolysaccharide (LPS)-stimulated normal human dermal fibroblasts (NHDF) to underline its application as an active ingredient in cosmetics. Methods: We assessed cell viability using MTT assay, so as to target any potential cytotoxicity of the extract. Moreover, with the aid of RT-qPCR, we studied the transcript accumulation of genes involved in cell antioxidant response, cell proliferation, and inflammation. Results: Our results revealed that the hydrolyzed rice flour-encapsulated (HRF) PT extract promotes anti-inflammatory and antioxidant response, increasing cell proliferation in NHDF cells. Conclusions: Our data indicate a promising use of HRF-encapsulated PT extract in cosmetics by reducing skin inflammation.
Dimitra Mosxou; Sophia Letsiou. Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts. Cosmetics 2021, 8, 76 .
AMA StyleDimitra Mosxou, Sophia Letsiou. Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts. Cosmetics. 2021; 8 (3):76.
Chicago/Turabian StyleDimitra Mosxou; Sophia Letsiou. 2021. "Exploring the Protective Effects of Phaeodactylum tricornutum Extract on LPS-Treated Fibroblasts." Cosmetics 8, no. 3: 76.
Cartilage acidic protein 1A (hCRTAC1-A) is an extracellular matrix protein (ECM) of human hard and soft tissue that is associated with matrix disorders. The central role of fibroblasts in tissue integrity and ECM health made primary human dermal fibroblasts (NHDF) the model for the present study, which aimed to provide new insight into the molecular function of hCRTAC1-A. Specifically, we explored the differential expression patterns of specific genes associated with the presence of hCRTAC1-A by RNA-seq and RT-qPCR analysis. Functional enrichment analysis demonstrated, for the very first time, that hCRTAC1-A is involved in extracellular matrix organization and development, through its regulatory effect on asporin, decorin, and complement activity, in cell proliferation, regeneration, wound healing, and collagen degradation. This work provides a better understanding of putative hCRTAC1-A actions in human fibroblasts and a fundamental insight into its function in tissue biology.
Sophia Letsiou; Manuel Manchado; Mariela Zografaki; Sofia Marka; Liliana Anjos; Dimitrios Skliros; Juan F. Martínez-Blanch; E. Flemetakis; Deborah M. Power. Deciphering the role of cartilage protein 1 in human dermal fibroblasts: a transcriptomic approach. Functional & Integrative Genomics 2021, 21, 503 -511.
AMA StyleSophia Letsiou, Manuel Manchado, Mariela Zografaki, Sofia Marka, Liliana Anjos, Dimitrios Skliros, Juan F. Martínez-Blanch, E. Flemetakis, Deborah M. Power. Deciphering the role of cartilage protein 1 in human dermal fibroblasts: a transcriptomic approach. Functional & Integrative Genomics. 2021; 21 (3-4):503-511.
Chicago/Turabian StyleSophia Letsiou; Manuel Manchado; Mariela Zografaki; Sofia Marka; Liliana Anjos; Dimitrios Skliros; Juan F. Martínez-Blanch; E. Flemetakis; Deborah M. Power. 2021. "Deciphering the role of cartilage protein 1 in human dermal fibroblasts: a transcriptomic approach." Functional & Integrative Genomics 21, no. 3-4: 503-511.
Parsley (Petroselinum crispum) aromatic, edible leaves are used fresh or dried as a herb, but also for their pharmaceutical properties. Given the profound concern to high nutritious and pharmaceutical value of superior plant’s germplasm, a survey is presented in this study on the phenotypic, genetic and phytochemical characterization of fifteen Greek parsley landraces in comparison to nine commercial cultivars, all grown under the same conditions in Greece. Our data reported high differences between the Greek landraces and most of the commercial cultivars in a majority of morphological traits investigated according to the International Union for the Protection of New Varieties of Plants descriptors list. The genetic diversity of all landraces and commercial cultivars were investigated using Inter Simple Sequence Repeat and Amplified Fragment Length Polymorphism molecular markers. The percentage of polymorphic loci was 63.83 % for the cultivars, and all loci were polymorphic (100 %) for the Greek landraces, while considerably higher values of unbiased haploid gene diversity were revealed in the landraces compared with the commercial cultivars. The essential oil yield of the landraces and cultivars of parsley studied ranged from 0.11−0.26 mL 100 g−1 leaf FW. The major compounds in the parsley essential oils as determined by gas-chromatography were α-pinene (0.6–11.8%), β-pinene (0.2–8.2%), myrcene (1.3–13.5%), β-phellandrene (9.6–39.1 %), terpinolene (1.4–8.9 %), 1, 3, 8-p-menthatriene (0.1–43.4 %), cis-carveol (0.2–8.1 %), iso-dihydro carveol acetate (0.2–6.9 %), myristicin (0.51–44.4%) and apiole (0.01–35.2 %). According to the targeted liquid chromatographic analysis coumarin derivatives, hydrocinnamic acids, flavanones, flavones and flavonols were identified in parsley methanolic extracts. Flavones were represented by apigenin and luteolin derivatives, and flavonols by kaempferol, quercetin, galangin and morin. Apiin was the major flavone in all parsley samples ranging from 1732.57 to 3676.43 mg 100 g -1 dry weight. The wide variation in phenotypic, genetic and phytochemical profiles observed for the landraces, indicate that the Greek germplasm collection could serve as an important source of genetic material for plant breeding and selection towards the development of new cultivars with superior traits regarding their aroma quality and polyphenolic content but also to support ongoing studies on flavonoid biosynthesis in this culinary herb.
Anastasia Boutsika; Eirini Sarrou; Catherine M. Cook; Ifigeneia Mellidou; Evangelia Avramidou; Andrea Angeli; Stefan Martens; Parthenopi Ralli; Sofia Letsiou; Anastasia Selini; Ioannis Grigoriadis; Nikos Tourvas; Kalliopi Kadoglidou; Apostolos Kalivas; Eleni Maloupa; Aliki Xanthopoulou; Ioannis Ganopoulos. Evaluation of parsley (Petroselinum crispum) germplasm diversity from the Greek Gene Bank using morphological, molecular and metabolic markers. Industrial Crops and Products 2021, 170, 113767 .
AMA StyleAnastasia Boutsika, Eirini Sarrou, Catherine M. Cook, Ifigeneia Mellidou, Evangelia Avramidou, Andrea Angeli, Stefan Martens, Parthenopi Ralli, Sofia Letsiou, Anastasia Selini, Ioannis Grigoriadis, Nikos Tourvas, Kalliopi Kadoglidou, Apostolos Kalivas, Eleni Maloupa, Aliki Xanthopoulou, Ioannis Ganopoulos. Evaluation of parsley (Petroselinum crispum) germplasm diversity from the Greek Gene Bank using morphological, molecular and metabolic markers. Industrial Crops and Products. 2021; 170 ():113767.
Chicago/Turabian StyleAnastasia Boutsika; Eirini Sarrou; Catherine M. Cook; Ifigeneia Mellidou; Evangelia Avramidou; Andrea Angeli; Stefan Martens; Parthenopi Ralli; Sofia Letsiou; Anastasia Selini; Ioannis Grigoriadis; Nikos Tourvas; Kalliopi Kadoglidou; Apostolos Kalivas; Eleni Maloupa; Aliki Xanthopoulou; Ioannis Ganopoulos. 2021. "Evaluation of parsley (Petroselinum crispum) germplasm diversity from the Greek Gene Bank using morphological, molecular and metabolic markers." Industrial Crops and Products 170, no. : 113767.
There is a persistent interest in innovative and multifunctional ingredients in biology research. With regards to this, natural sources have an important role due to their multiple benefits. Thus, this study aims to present the pleiotropic activity of Prunus avium L. extract on human primary fibroblasts for proving its efficacy in dermis-related processes. We focused on the safety and efficacy assessments based on cytotoxicity and gene expression analysis under oxidative stress. Specifically, Prunus avium L. extract was proved non-cytotoxic in human fibroblasts. The gene expression analysis unveiled that this extract has in vitro protective properties on human dermal fibroblasts under oxidative stress related to antioxidant activity, anti-inflammatory response, cell proliferation and cell- aging. Our study demonstrated for the very first time that the Prunus avium L. extract is a multifunctional ingredient as it mediates several human dermis-related in vitro processes highlighting its potential to be used as an active ingredient in skin care products.
Sophia Letsiou; Aggeliki Karamaouna; Ioannis Ganopoulos; Aliki Kapazoglou; Aliki Xanthopoulou; Eirini Sarrou; Georgia Tanou; Athanasios Molassiotis. The pleiotropic effects of Prunus avium L. extract against oxidative stress on human fibroblasts. An in vitro approach. Molecular Biology Reports 2021, 48, 4441 -4448.
AMA StyleSophia Letsiou, Aggeliki Karamaouna, Ioannis Ganopoulos, Aliki Kapazoglou, Aliki Xanthopoulou, Eirini Sarrou, Georgia Tanou, Athanasios Molassiotis. The pleiotropic effects of Prunus avium L. extract against oxidative stress on human fibroblasts. An in vitro approach. Molecular Biology Reports. 2021; 48 (5):4441-4448.
Chicago/Turabian StyleSophia Letsiou; Aggeliki Karamaouna; Ioannis Ganopoulos; Aliki Kapazoglou; Aliki Xanthopoulou; Eirini Sarrou; Georgia Tanou; Athanasios Molassiotis. 2021. "The pleiotropic effects of Prunus avium L. extract against oxidative stress on human fibroblasts. An in vitro approach." Molecular Biology Reports 48, no. 5: 4441-4448.
Sophia Letsiou. Tracing skin aging process: a mini- review of in vitro approaches. Biogerontology 2021, 22, 261 -272.
AMA StyleSophia Letsiou. Tracing skin aging process: a mini- review of in vitro approaches. Biogerontology. 2021; 22 (3):261-272.
Chicago/Turabian StyleSophia Letsiou. 2021. "Tracing skin aging process: a mini- review of in vitro approaches." Biogerontology 22, no. 3: 261-272.
Propolis is a resinous substance produced by bees that exhibits antimicrobial, immunostimulatory and antioxidant activity. Its use is common in functional foods, cosmetics and traditional medicine despite the fact that it demonstrates low extraction yields and inconsistency in non-toxic solvents. In this work, a new encapsulation and delivery system consisting of liposomes and cyclodextrins incorporating propolis polyphenols has been developed and characterized. The antioxidant, antimutagenic and antiaging properties of the system under normal and UVB-induced oxidative stress conditions were investigated in cultured skin cells and/or reconstituted skin model. Furthermore, the transcript accumulation for an array of genes involved in many skin-related processes was studied. The system exhibits significant polyphenol encapsulation efficiency, physicochemical stability as well as controlled release rate in appropriate conditions. The delivery system can retain the anti-mutagenic, anti-oxidative and anti-ageing effects of propolis polyphenols to levels similar and comparable to those of propolis methanolic extracts, making the system ideal for applications where non-toxic solvents are required and controlled release of the polyphenol content is desired.
Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants 2021, 10, 420 .
AMA StyleEleni Spanidi, Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Sophia Letsiou, Nektarios Aligiannis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Ioannis Mourtzinos, Aglaia Pappa, Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants. 2021; 10 (2):420.
Chicago/Turabian StyleEleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. 2021. "A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications." Plants 10, no. 2: 420.
Natural ingredients have been used to improve the state of health in humans. The genus Paeonia has been studied only limited yet it’s reported to have many activities such as antioxidant and anti-inflammatory. To this context, here we focused on an endemic Paeonia species in Attica. This study aims to present the development of the Paeonia mascula subsp. hellenica callus extract and its pleiotropic bioactivity on human primary keratinocytes exploring its potential application as an active agent in skin-related products. This extract showed a high scavenging activity with high phenolic content and an interesting metabolic profile. At a molecular level, the study on the transcript accumulation of genes revealed that this extract exhibits in vitro skin-related protection properties by mediating mitochondrial energy, cell proliferation, immune and inflammatory response and positively regulates genes involved in epidermal and in stratum corneum function. Besides, the extract is proven not skin irritant on reconstructed human skin model. These findings indicate that the specific P. mascula subsp. hellenica extract possesses significant in vitro protection activity on human epidermis and provides new insights into its beneficial role in skin confirming that the advent of biotechnology contribution the past few decades.
Sophia Letsiou; Artemis Bakea; Anna Holefors; Jadwiga Rembiesa. In vitro protective effects of Paeonia mascula subsp. hellenica callus extract on human keratinocytes. Scientific Reports 2020, 10, 1 -11.
AMA StyleSophia Letsiou, Artemis Bakea, Anna Holefors, Jadwiga Rembiesa. In vitro protective effects of Paeonia mascula subsp. hellenica callus extract on human keratinocytes. Scientific Reports. 2020; 10 (1):1-11.
Chicago/Turabian StyleSophia Letsiou; Artemis Bakea; Anna Holefors; Jadwiga Rembiesa. 2020. "In vitro protective effects of Paeonia mascula subsp. hellenica callus extract on human keratinocytes." Scientific Reports 10, no. 1: 1-11.
The strain Aspergillus chevalieri TM2-S6 was isolated from the sponge Axinella and identified according to internal transcribed spacer (ITS) molecular sequence homology with Aspergillus species from the section Restricti. The strain was cultivated 9 days on potato dextrose broth (PDB), and the medium evaluated as antioxidant on primary normal human dermal fibroblasts (NHDF). The cultivation broth was submitted to sterile filtration, lyophilized and used without any further processing to give the Aspergillus chevalieri TM2-S6 cultivation broth ingredient named ACBB. ACCB contains two main compounds: tetrahydroauroglaucin and flavoglaucin. Under oxidative stress, ACCB showed a significant promotion of cell viability. To elucidate the mechanism of action, the impact on a panel of hundreds of genes involved in fibroblast physiology was evaluated. Thus, ACCB stimulates cell proliferation (VEGFA, TGFB3), antioxidant response (GPX1, SOD1, NRF2), and extracellular matrix organization (COL1A1, COL3A1, CD44, MMP14). ACCD also reduced aging (SIRT1, SIRT2, FOXO3). These findings indicate that Aspergillus chevalieri TM2-S6 cultivation broth exhibits significant in vitro skin protection of human fibroblasts under oxidative stress, making it a potential cosmetic ingredient.
Sophia Letsiou; Artemis Bakea; Géraldine Le Goff; Philippe Lopes; Konstantinos Gardikis; Michal Weis; Yehuda Benayahu; Jamal Ouazzani. Marine Fungus Aspergillus chevalieri TM2-S6 Extract Protects Skin Fibroblasts from Oxidative Stress. Marine Drugs 2020, 18, 460 .
AMA StyleSophia Letsiou, Artemis Bakea, Géraldine Le Goff, Philippe Lopes, Konstantinos Gardikis, Michal Weis, Yehuda Benayahu, Jamal Ouazzani. Marine Fungus Aspergillus chevalieri TM2-S6 Extract Protects Skin Fibroblasts from Oxidative Stress. Marine Drugs. 2020; 18 (9):460.
Chicago/Turabian StyleSophia Letsiou; Artemis Bakea; Géraldine Le Goff; Philippe Lopes; Konstantinos Gardikis; Michal Weis; Yehuda Benayahu; Jamal Ouazzani. 2020. "Marine Fungus Aspergillus chevalieri TM2-S6 Extract Protects Skin Fibroblasts from Oxidative Stress." Marine Drugs 18, no. 9: 460.
Adverse environmental conditions such as UV radiation induce oxidative and aging events leading to severe damage to human skin cells. Natural products such as plant extracts have been implicated in the skin anti-oxidant and anti-aging cellular protection against environmental stress. Moreover, environmental factors have been shown to impact chromatin structure leading to altered gene expression programs with profound changes in cellular functions. In this study, we assessed the in vitro effect of a leaf extract from Vitis vinifera L. on UV-stressed primary human dermal fibroblasts, focusing on gene expression and DNA methylation as an epigenetic factor. Expression analysis of two genes known to be implicated in skin anti-aging, SIRT1and HSP4, demonstrated significant induction in the presence of the extract under normal or UVA conditions. In addition, DNA methylation profiling of SIRT1 and HSP47 promoters showed that the V. vinifera L. extract induced changes in the DNA methylation pattern of both genes that may be associated with SIRT1 and HSP47 gene expression. Our study shows for the first time transcriptional and DNA methylation alterations on human skin fibroblasts exposed to UV stress and suggest a protective effect of a V. vinifera extract possibly through transcriptional regulation of critical skin anti-aging genes.
Sophia Letsiou; Aliki Kapazoglou; Athanasios Tsaftaris. Transcriptional and epigenetic effects of Vitis vinifera L. leaf extract on UV-stressed human dermal fibroblasts. Molecular Biology Reports 2020, 47, 1 -10.
AMA StyleSophia Letsiou, Aliki Kapazoglou, Athanasios Tsaftaris. Transcriptional and epigenetic effects of Vitis vinifera L. leaf extract on UV-stressed human dermal fibroblasts. Molecular Biology Reports. 2020; 47 (8):1-10.
Chicago/Turabian StyleSophia Letsiou; Aliki Kapazoglou; Athanasios Tsaftaris. 2020. "Transcriptional and epigenetic effects of Vitis vinifera L. leaf extract on UV-stressed human dermal fibroblasts." Molecular Biology Reports 47, no. 8: 1-10.