Basil (Ocimum basilicum L.) is an essential ingredient of the Mediterranean cuisine due to its distinctive aroma. Genovese basil leaves are used to prepare “pesto”, a condiment that has always caught the interest of consumers and producers. Usually, basil for industrial processing is harvested more than once to extract a higher yield. However, successive cuts can affect quality traits that play a crucial role in defining the product’s final sensory profile. This research was aimed to evaluate the impact of cut on the quantitative and qualitative properties of three Genovese basil cultivars (Aroma 2, Eleonora and Italiano Classico) grown in an open field. Nitrate content, phenolic acids and aromatic profile were determined by ion chromatography (IC), high-performance liquid chromatography (HPLC), and gas chromatography coupled to a mass spectrometer (GC/MS) analysis, respectively. The second harvest increased fresh biomass and total phenolic acids content by 172% and 413%, respectively, with Italiano Classico recording the highest values. The combination of second-cut Aroma 2 yielded the lowest nitrate (473.8 mg kg⁻¹ of fresh weight) and Eugenol (2.4%) levels. In the second harvest, Eleonora showed an increase in eugenol and trans-α-bergamotene of 75.3% and 48.2%, respectively; whereas, eucalyptol and β-cis-ocimene decreased by 34.4% and 51.6%, respectively. Although successive harvests may increase basil yield and quality overall, the cultivar-dependent response to successive cuts needs to be accounted for in order to accomplish standardization of industrial “pesto” sauce.

Fresh vegetables and fruits have always been the mainstays of good nutrition as providers of fiber, beneficial phytochemicals (such as vitamins and phenolic compounds), and minerals. Today and in the future, biofortification is a promising strategy to increase the concentration of these compounds. Considering the importance of minerals in human health, the enrichment of fresh produce for consumption has been considered through specific agronomic approaches. This review discusses, in detail, the latest findings on vegetable agronomic biofortification, aimed at increasing the concentration of crucial minerals, such as iron (Fe), zinc (Zn), iodine (I), selenium (Se), molybdenum (Mo), and silicon (Si), in edible portions, focusing on the direct and indirect effects of this strategy. Although agronomic biofortification is considered a feasible technique, the approach is complex due to the many interactions between the microelement bioavailability for both plants and consumers. Therefore, the effects of biofortification on human health and the influence of beneficial and antinutritional compounds were discussed in detail to analyze the advantages and disadvantages of this practice.

A promising strategy for sustainably increasing the quality and yield of horticultural products is the use of natural plant biostimulants. In this work, through a greenhouse experiment, we evaluated the effect of a legume-derived biostimulant at three dose treatments (0.0 control, 2.5 mL L⁻¹, and 5.0 mL L⁻¹) on the yield performance, nutrients traits, leaf anatomical traits, gas exchanges, and carbon photosynthetic assimilation of greenhouse lettuce. The lettuce plants were foliar sprayed every 7 days for 5 weeks. The application of plant biostimulant, at both lower and higher dosages, increased the nutrient use efficiency, root dry weight, and leaf area. However, it is noteworthy that the 5.0 mL L⁻¹ dose enhanced photosynthetic activity in the early phase of growth (15 DAT), thus supplying carbon skeletons useful for increasing the number of leaves and their efficiency (higher SPAD), and for boosting nutrient uptake (P, S, and K) and transport to leaves, while the 2.5 mL L⁻¹ dose exerted specific effects on roots, increasing their dimension and enabling them to better use nitrate and Ca. A higher dose of biostimulant application might find its way in shorter growing cycle, thus presenting new horizons for new lines of research in baby leaves production.

Hydroponics growing systems often contain excessive nutrients (especially nitrates), which could lead to a quality loss in ready-to-eat leafy vegetables and posing a health risk to consumers, if managed inadequately. A floating raft system was adopted to assay the production and quality performance of lettuce (Lactuca sativa L. cv ‘Maravilla De Verano Canasta’) deprived of the nutrient solution by replacement with only water, three and six days before harvest. Yield and quality parameters, mineral composition, pigments, organic acids, amino acids profile, soluble proteins, and carbohydrate content were determined. Nutrient solution deprivation six days before harvest resulted in a significant reduction in leaf nitrate (−53.3%) concomitant with 13.8% of yield loss, while plants deprived of nutrient solution three days before harvest increased total phenols content (32.5%) and total ascorbic acid (102.1%), antioxidant activity (82.7%), anthocyanins (7.9%), sucrose (38.9%), starch (19.5%), and γ-aminobutyric acid (GABA; 28.2%), with a yield reduction of 4.7%, compared to the control. Our results suggest that nutrient solution deprivation three days before harvest is a successful strategy to reduce nitrate content and increase the nutritional quality of lettuce grown in floating raft systems with negligible impact on yield. These promising results warrant further investigation of the potential effect of nutrient solution deprivation on the quality attributes of other leafy vegetables cultivated in floating raft systems and in a “cascade” growing system.

As part of the European Green Deal, the Farm to Fork strategy was introduced with the idea that environment, agriculture and food are interconnected topics. Reducing the use of synthetic fertilizers by 20% before 2030 through the adoption of circular economy principles is one of the goals to be achieved. There are several bioproducts that can be obtained from the valorization of agro-industrial wastes used to increase crop yields under low-fertilizer applications. However, the aim of this review is to describe production methods and the use of compost tea on horticultural crops to understand its real potential in providing plant growth support. The effects of compost tea on crops can vary widely depending on the waste material used, compost quality, compost tea production process and parameters, and the interaction between horticultural species and compost tea application dose. Therefore, because of this heterogeneity, it is possible that we would achieve real, positive impacts on the environment and horticultural production if there were more collaboration between the research sector and private farms. This collaboration would allow the development of protocols for compost tea production and customized use according to real farm needs. This would reduce both the costs associated with the disposal of waste produced on the farm and reduce the costs associated with the supply of synthetic fertilizers. The adoption of on-farm guidelines for compost tea use would achieve a balanced trade-off between agricultural productivity and environmental sustainability. The literature review shows that the most-used dilution ratios, regardless of the type of starting compost, range from 1:5 to 1:10 compost–water (v/v). Although a complete understanding of the biostimulatory mechanisms activated by compost tea is lacking, the application of this bioproduct would improve the physiological and productive performance of many horticultural species of interest, especially under suboptimal conditions such as organic production.

Climate changes have exacerbated the progression of drought conditions on a global scalethreating to crop production and heightening concerns over food security. Water scarcity enforces alterations in fundamental morphology, physiology and biochemical traits in crops. Consequently, it is imperative to identify environmentally sustainable alternative solutions to mitigate this problem and enhance overall plant performance. In this sense, biostimulants have emerged as a promising alternative as they improve plant resilience, enhance physiological processes, and mitigate the detrimental consequences of water deficit conditions on crop production. This review compiles the latest research on the application of organic extracts and inorganic compounds in crops subjected to drought conditions, specifically humic acids, protein hydrolysates, seaweed extracts, and silicon. Moreover, it offers a comprehensive overview of the origins and effectiveness of these biostimulants, with a detailed analysis of their application and the associated physiological, biochemical, and genetic modifications induced by these bioactive compounds. This knowledge enhances the understanding of the efficacy and implementation strategies pertinent of these compounds under water stress scenarios in agricultural settings.