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Liang Chen
School of Life Sciences, Jiangsu University, Zhenjiang 212013, China

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Journal article
Published: 19 July 2021 in Processes
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Diabetes mellitus (DM), as a chronic disease caused by insulin deficiency or using obstacles, is gradually becoming a principal worldwide health problem. Pueraria lobata is one of the traditional Chinese medicinal and edible plants, playing roles in improving the cardiovascular system, lowering blood sugar, anti-inflammation, anti-oxidation, and so on. Studies on the hypoglycemic effects of Pueraria lobata were also frequently reported. To determine the active ingredients and related targets of Pueraria lobata for DM, 256 metabolites were identified by LC/MS non targeted metabonomics, and 19 active ingredients interacting with 51 DM-related targets were screened. The results showed that puerarin, quercetin, genistein, daidzein, and other active ingredients in Pueraria lobata could participate in the AGE-RAGE signaling pathway, insulin resistance, HIF-1 signaling pathway, FoxO signaling pathway, and MAPK signaling pathway by acting on VEGFA, INS, INSR, IL-6, TNF and AKT1, and may regulate type 2 diabetes, inflammation, atherosis and diabetes complications, such as diabetic retinopathy, diabetic nephropathy, and diabetic cardiomyopathy.

ACS Style

Shu Zhang; Qi Ge; Liang Chen; Keping Chen. Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology. Processes 2021, 9, 1245 .

AMA Style

Shu Zhang, Qi Ge, Liang Chen, Keping Chen. Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology. Processes. 2021; 9 (7):1245.

Chicago/Turabian Style

Shu Zhang; Qi Ge; Liang Chen; Keping Chen. 2021. "Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology." Processes 9, no. 7: 1245.

Review article
Published: 18 March 2021 in Journal of Food Quality
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Over the past decade, oxidative stress was shown to be a key factor for various diseases. The term “antioxidant” also rapidly gained attention worldwide, viewed as beneficial in disease prevention. Resveratrol (RSV), a natural polyphenol, is a plant antitoxin formed in response to harmful environmental factors such as infection and injury. This antitoxin is found in grapes, strawberries, peanuts, or herbal medicines and exhibits many pharmacological effects involved in antitumor, anti-inflammatory, antiaging, and antioxidation stress mechanisms. Recently, numerous in vitro and in vivo experiments have shown that RSV harbors antioxidative stress properties and can be used as an antioxidant. Here, we review the free radical scavenging ability, antioxidant properties, signaling pathways, expression and regulation of antioxidant enzymes, and oxidative stress-related diseases associated with RSV.

ACS Style

Tongyu Gu; Nianmin Wang; Tong Wu; Qi Ge; Liang Chen. Antioxidative Stress Mechanisms behind Resveratrol: A Multidimensional Analysis. Journal of Food Quality 2021, 2021, 1 -12.

AMA Style

Tongyu Gu, Nianmin Wang, Tong Wu, Qi Ge, Liang Chen. Antioxidative Stress Mechanisms behind Resveratrol: A Multidimensional Analysis. Journal of Food Quality. 2021; 2021 ():1-12.

Chicago/Turabian Style

Tongyu Gu; Nianmin Wang; Tong Wu; Qi Ge; Liang Chen. 2021. "Antioxidative Stress Mechanisms behind Resveratrol: A Multidimensional Analysis." Journal of Food Quality 2021, no. : 1-12.

Article
Published: 06 July 2020 in Molecular and Cellular Biochemistry
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Food additives are widely used in various food products to preserve the taste, color, and other qualities. However, if they are used improperly or exceed the standard, they will cause damage to the human body. Sulfite is a commonly used food additive to prevent oxidation from deteriorating the nutrients in foods, it has been widely used as a bleaching agent in the food industry for a long time. In this study, human hepatocytes L02 cells were used as a model cell line to evaluate the toxicity of sodium sulfite. The cell morphology and cell proliferation were affected by sodium sulfite treatment, and apoptosis was detected. Transcriptome sequencing showed 97 differentially expressed genes (DEGs) between the experimental group (IC50) and the control group (MOCK), and 27 differentially expressed genes related to cell apoptosis, metabolism and inflammation were selected for validation by qPCR. Among them, 13 significantly upregulated genes and 14 significantly downregulated genes were identified by qPCR. The results showed that with increase of sodium sulfite concentration, the morphology of L02 changed, cell proliferation and activity were inhibited, and sodium sulfite caused apoptosis in a concentration- and time-dependent manner. The resulting toxic mechanism inhibits proliferation, damages the mitochondrial integrity, and promotes apoptosis.

ACS Style

Xu Han; Feifei Zhu; Liang Chen; Hong Wu; Tao Wang; Keping Chen. Mechanism analysis of toxicity of sodium sulfite to human hepatocytes L02. Molecular and Cellular Biochemistry 2020, 473, 25 -37.

AMA Style

Xu Han, Feifei Zhu, Liang Chen, Hong Wu, Tao Wang, Keping Chen. Mechanism analysis of toxicity of sodium sulfite to human hepatocytes L02. Molecular and Cellular Biochemistry. 2020; 473 (1-2):25-37.

Chicago/Turabian Style

Xu Han; Feifei Zhu; Liang Chen; Hong Wu; Tao Wang; Keping Chen. 2020. "Mechanism analysis of toxicity of sodium sulfite to human hepatocytes L02." Molecular and Cellular Biochemistry 473, no. 1-2: 25-37.

Journal article
Published: 13 December 2019 in Toxins
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Resveratrol, a natural polyterpenoid, can scavenge reactive oxygen species in vivo to carry out the functions of antioxidation and antiaging. Resveratrol’s anti-cancer capability has attracted widespread attention, but its molecular mechanism has not been systematically explained. In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. A total of 330 significantly differential genes were identified through large-scale transcriptome sequencing, among which 103 genes were upregulated and 227 genes were downregulated. Transcriptome and qRT-PCR data proved that a large number of genes related to cell cycle were differentially expressed after the treatment of resveratrol. The changes of cell cycle phases at different time points after treating with resveratrol were further detected, and it was found that the cells were arrested in the S phase because of the percentage of cells in S phase increased and cells in G1/G0 phase decreased. In conclusion, resveratrol can inhibit the proliferation of 4T1 cancer cells by inhibiting cell cycle and inducing apoptosis.

ACS Style

Hong Wu; Liang Chen; Feifei Zhu; Xu Han; Lindan Sun; Keping Chen. The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells. Toxins 2019, 11, 731 .

AMA Style

Hong Wu, Liang Chen, Feifei Zhu, Xu Han, Lindan Sun, Keping Chen. The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells. Toxins. 2019; 11 (12):731.

Chicago/Turabian Style

Hong Wu; Liang Chen; Feifei Zhu; Xu Han; Lindan Sun; Keping Chen. 2019. "The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells." Toxins 11, no. 12: 731.

Journal article
Published: 01 September 2019 in Journal of Ethnopharmacology
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Potentilla discolor Bunge (PDB) is a commonly used herbal for alleviating diabetes mellitus and its complications. Although accumulating evidences show the anti-diabetic efficacy of PDB, the vital anti-diabetic compounds and their functional targets remain elusive. To investigate the anti-diabetic ingredients and their functional mechanisms in PDB, gas chromatograph-mass spectrometry analysis was performed on PDB extract and 21 were testified as anti-diabetic compounds. Subsequently their potential protein targets were also identified. The bioinformatics analysis was implemented by network pharmacology-based approaches. STRING analysis was performed to reveal enrichment of these target proteins, protein-protein interactions, pathways and related diseases. Cytoscape was used to determine the potential protein targets for these components in PDB, indicating that 21 anti-diabetic compounds in PDB regulate 33 diabetes-related proteins in 28 signal pathways and involve 21 kinds of diabetes-related diseases. Among the 21 potential anti-diabetic components predicted by network analysis, tricetin was firstly experimentally validated at the molecular and cellular level. Results indicated that this active small-molecule compound may have beneficial effects on improving glucose uptake. We envisage that network analysis will be useful in screening bioactive compounds of medicinal plants.

ACS Style

NianNian Wang; Feifei Zhu; Mingxiang Shen; Lipeng Qiu; Min Tang; Hengchuan Xia; Liang Chen; Yi Yuan; Shangshang Ma; Keping Chen. Network pharmacology-based analysis on bioactive anti-diabetic compounds in Potentilla discolor bunge. Journal of Ethnopharmacology 2019, 241, 111905 .

AMA Style

NianNian Wang, Feifei Zhu, Mingxiang Shen, Lipeng Qiu, Min Tang, Hengchuan Xia, Liang Chen, Yi Yuan, Shangshang Ma, Keping Chen. Network pharmacology-based analysis on bioactive anti-diabetic compounds in Potentilla discolor bunge. Journal of Ethnopharmacology. 2019; 241 ():111905.

Chicago/Turabian Style

NianNian Wang; Feifei Zhu; Mingxiang Shen; Lipeng Qiu; Min Tang; Hengchuan Xia; Liang Chen; Yi Yuan; Shangshang Ma; Keping Chen. 2019. "Network pharmacology-based analysis on bioactive anti-diabetic compounds in Potentilla discolor bunge." Journal of Ethnopharmacology 241, no. : 111905.

Journal article
Published: 01 May 2019 in Ecotoxicology and Environmental Safety
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The widespread use of silver nanoparticles (AgNPs) has raised public concern due to their potential toxic effects on humans and the environment. Although some studies have evaluated the toxicity of nanomaterials in vertebrates, studies on their hazardous effects on insects are limited. Here we focused on different concentrations of AgNPs to silkworms, a promising model organism, to evaluate their toxic effects by omics analysis. After the silkworms were fed with 100 mg L-1 AgNPs, transcriptomics analysis showed differential expression of 43 genes: 39 upregulated and 4 downregulated. These differentially expressed genes (DEGs) were involved in the digestion process, various metabolic pathways, transmembrane transport and energy synthesis. Proteomic results for silkworms fed with 400 mg L-1 AgNPs revealed 14 significantly differentially expressed proteins: 11 downregulated and 3 upregulated. Reverse transcription-polymerase chain reaction (RT-PCR) results showed that the expression levels of eight proteins were similar to the transcription levels of their corresponding genes. As the AgNPs concentration was increased, the expression of digestive enzymes was downregulated, which damaged the silkworm tissue and suppressed the activity of the enzyme superoxide dismutase and the protein HSP 1, causing oxidative stress and the production of reactive oxygen species, which had toxic effects on the silkworm digestive system. Histopathological results showed that treatment with 400 mg L-1 AgNPs destroyed the basal lamina and the columnar cells, caused adverse effects on tissues and had the potential to induce harmful effects on the digestive system. The data presented herein provide valuable information on the hazards and risks of nanoparticle contamination. Main finding: AgNPs would downregulate some digestive enzymes, damage the tissue of midgut in silkworm, meantime induce the accumulation of reactive oxygen species which may cause oxidative stress.

ACS Style

Liang Chen; Xu Meng; Jie Gu; Weiqiang Fan; Nouara Abdlli; Frank Addai Peprah; NianNian Wang; Feifei Zhu; Peng Lü; Shangshang Ma; Keping Chen. Silver nanoparticle toxicity in silkworms: Omics technologies for a mechanistic understanding. Ecotoxicology and Environmental Safety 2019, 172, 388 -395.

AMA Style

Liang Chen, Xu Meng, Jie Gu, Weiqiang Fan, Nouara Abdlli, Frank Addai Peprah, NianNian Wang, Feifei Zhu, Peng Lü, Shangshang Ma, Keping Chen. Silver nanoparticle toxicity in silkworms: Omics technologies for a mechanistic understanding. Ecotoxicology and Environmental Safety. 2019; 172 ():388-395.

Chicago/Turabian Style

Liang Chen; Xu Meng; Jie Gu; Weiqiang Fan; Nouara Abdlli; Frank Addai Peprah; NianNian Wang; Feifei Zhu; Peng Lü; Shangshang Ma; Keping Chen. 2019. "Silver nanoparticle toxicity in silkworms: Omics technologies for a mechanistic understanding." Ecotoxicology and Environmental Safety 172, no. : 388-395.

Review article
Published: 19 September 2018 in Life Sciences
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Type 2 diabetes mellitus (T2DM) is one of the most common diseases of endocrine and metabolic disorders, whose mechanism is still largely unknown. Fortunately, various “omics” tools have been employed to better understand the progression pathologies of T2DM and its complications. More specifically, proteomics, metabolomics and metagenomics have played crucial roles in advancing deeper understanding of the physiological processes and regulatory mechanisms of T2DM, such as regulation of signaling pathways perturbed by glucose levels, intestinal microorganism, and inflammation and so on. By analyzing the dynamic change and modification of proteins, proteomics has become an important tool in biology and medicine. Metabolomic analysis can amplify and quantify metabolites in living organisms to reveal the relative relationship between metabolites and physiological and pathological changes. There are also increasing evidences that the human microbiome, specifically the gastrointestinal microbiome have a potential role in the etiology and pathological outcomes of T2DM and its complications. This article summarized and discussed the recent applications of these “omics” tools in finding biomarkers for T2DM and its complications. We also reviewed employing multiple “omics” to further advance our understanding of this pathology. This review will benefit deeper understanding in new therapeutic and/or diagnostic biological target for the discovery of T2DM and its complications.

ACS Style

NianNian Wang; Feifei Zhu; Liang Chen; Keping Chen. Proteomics, metabolomics and metagenomics for type 2 diabetes and its complications. Life Sciences 2018, 212, 194 -202.

AMA Style

NianNian Wang, Feifei Zhu, Liang Chen, Keping Chen. Proteomics, metabolomics and metagenomics for type 2 diabetes and its complications. Life Sciences. 2018; 212 ():194-202.

Chicago/Turabian Style

NianNian Wang; Feifei Zhu; Liang Chen; Keping Chen. 2018. "Proteomics, metabolomics and metagenomics for type 2 diabetes and its complications." Life Sciences 212, no. : 194-202.

Primary research
Published: 04 September 2018 in Cancer Cell International
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MicroRNAs are small RNAs (~ 22 nt) that modulate the expression of thousands of genes in tumors and play important roles in the formation of multidrug resistance. In this study, we firstly investigated that miR-4532 involved in the multidrug resistance formation of breast cancer by targeting hypermethylated in cancer 1 (HIC-1), a tumor-suppressor gene. To identify and characterize the possible miRNAs in regulating multidrug resistance, we employed the transcriptome sequencing approach to profile the changes in the expression of miRNAs and their target mRNAs were obtained by bioinformatics prediction. Then the molecular biology experiments were conducted to confirm miR-4532 involved in multidrug resistance formation of breast cancer. The luciferase reporter assay experiment was employed to confirm that HIC-1 was the target of miR-4532. Transfection with an miR-4532 mimic indicated miR-4532 mimic significantly increased breast cancer cell resistance to adriamycin. Cell proliferation and invasion assay experiments showed overexpression of HIC-1 inhibited the invasion and metastasis of breast cancer cells. Meanwhile, the interleukin (IL)-6/signal transducer and activator of transcription 3 (STAT3) signaling pathway was confirmed to be involving in multidrug resistance by western blotting experiments. These results suggest that downregulation of hypermethylated in cancer-1 by miR-4532 could promote adriamycin resistance in breast cancer cells, in which the IL-6/STAT3 pathway was regulated by the HIC-1. This finding might contribute to new therapeutic target for reversal of tumor resistance.

ACS Style

Fan Feng; Xiaolan Zhu; Chunyan Wang; Liang Chen; Weiping Cao; Yueqin Liu; Qi Chen; Wenlin Xu. Downregulation of hypermethylated in cancer-1 by miR-4532 promotes adriamycin resistance in breast cancer cells. Cancer Cell International 2018, 18, 127 .

AMA Style

Fan Feng, Xiaolan Zhu, Chunyan Wang, Liang Chen, Weiping Cao, Yueqin Liu, Qi Chen, Wenlin Xu. Downregulation of hypermethylated in cancer-1 by miR-4532 promotes adriamycin resistance in breast cancer cells. Cancer Cell International. 2018; 18 (1):127.

Chicago/Turabian Style

Fan Feng; Xiaolan Zhu; Chunyan Wang; Liang Chen; Weiping Cao; Yueqin Liu; Qi Chen; Wenlin Xu. 2018. "Downregulation of hypermethylated in cancer-1 by miR-4532 promotes adriamycin resistance in breast cancer cells." Cancer Cell International 18, no. 1: 127.

Journal article
Published: 01 May 2018 in Environmental Pollution
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Accumulation of excess fluoride has a destructive effect on the environment, endangering human health, affecting organism growth and development, and leading to damage to the biological chain, thereby affecting ecological environment balance. In recent years, numerous studies focused on the molecular mechanisms associated with fluoride toxicity; however, fluoride-toxicity mechanisms in insect cells remain unclear. This study explored the toxic impact of sodium fluoride (NaF) on Spodoptera frugiperda 9 (Sf9) insect cells. High concentrations of NaF (10−4 M, 10−3 M and 10−2 M) resulted in cell enlargement, cell membrane blurring and breakage, and release of cellular contents. Dose-response curves indicated that NaF-specific inhibition rates on Sf9-cell activity increased along with increases in NaF concentration, with a half-inhibitory concentration (IC50) for NaF of 5.919 × 10−3 M at 72 h. Compared with controls, the percentages of early and late apoptotic and necrotic cells clearly increased based on observed increases in NaF concentrations. Two-dimensional gel electrophoresis combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to detect differentially expressed proteins in Sf9 cells treated with IC50 NaF, identifying 17 proteins, seven of which were upregulated and 10 downregulated. These results demonstrated that Sf9 cells showed signs of NaF-mediated toxicity through alterations in cell morphology, apoptosis rates, and protein expression.

ACS Style

Huan Zuo; Liang Chen; Ming Kong; Yanhua Yang; Peng Lü; Lipeng Qiu; Qiang Wang; Shangshang Ma; Keping Chen. The toxic effect of sodium fluoride on Spodoptera frugiperda 9 cells and differential protein analysis following NaF treatment of cells. Environmental Pollution 2018, 236, 313 -323.

AMA Style

Huan Zuo, Liang Chen, Ming Kong, Yanhua Yang, Peng Lü, Lipeng Qiu, Qiang Wang, Shangshang Ma, Keping Chen. The toxic effect of sodium fluoride on Spodoptera frugiperda 9 cells and differential protein analysis following NaF treatment of cells. Environmental Pollution. 2018; 236 ():313-323.

Chicago/Turabian Style

Huan Zuo; Liang Chen; Ming Kong; Yanhua Yang; Peng Lü; Lipeng Qiu; Qiang Wang; Shangshang Ma; Keping Chen. 2018. "The toxic effect of sodium fluoride on Spodoptera frugiperda 9 cells and differential protein analysis following NaF treatment of cells." Environmental Pollution 236, no. : 313-323.