During the Eocene-Oligocene transition, Earth cooled significantly from a greenhouse to an icehouse climate. Nannofossil assemblages from Southern Ocean sites enable evaluation of paleoceanographic changes and, hence, of the oceanic response to Antarctic ice sheet evolution during the Eocene and Oligocene. A combination of environmental factors such as sea surface temperature and nutrient availability is recorded by the nannofossil assemblages of and can be interpreted as responses to the following changes. A cooling trend, started in the Middle Eocene, was interrupted by warming during the Middle Eocene Climatic optimum and by short cooling episodes. The cooling episode at 39.6 Ma preceded a shift toward an interval that was dominated by oligotrophic nannofossil assemblages from ~39.1 to ~36.2 Ma. We suggest that oligotrophic conditions were associated with increased water mass stratification, low nutrient contents, and high efficiency of the oceanic biological pump that, in turn, promoted sequestration of carbon from surface waters, which favored cooling. After 36.2 Ma, we document a large synchronous surface water productivity turnover with a dominant eutrophic nannofossil assemblage that was accompanied by a pronounced increase in magnetotactic bacterial abundance. This turnover reflects a response of coccolithophorids to changed nutrient inputs that was likely related to partial deglaciation of a transient Antarctic ice sheet and/or to iron delivery to the sea surface. Eutrophic conditions were maintained throughout the Oligocene, which was characterized by a nannofossil assemblage shift toward cool conditions at the Eocene-Oligocene transition. Finally, a warm nannofossil assemblage in the Late Oligocene indicates a warming phase.

The Palaeocene-Eocene Thermal Maximum (ca. 56 million years ago) offers a primary analogue for future global warming and carbon cycle recovery. Yet, where and how massive carbon emissions were mitigated during this climate warming event remains largely unknown. Here we show that organic carbon burial in the vast epicontinental seaways that extended over Eurasia provided a major carbon sink during the Palaeocene-Eocene Thermal Maximum. We coupled new and existing stratigraphic analyses to a detailed paleogeographic framework and using spatiotemporal interpolation calculated ca. 720–1300 Gt organic carbon excess burial, focused in the eastern parts of the Eurasian epicontinental seaways. A much larger amount (2160–3900 Gt C, and when accounting for the increase in inundated shelf area 7400–10300 Gt C) could have been sequestered in similar environments globally. With the disappearance of most epicontinental seas since the Oligocene-Miocene, an effective negative carbon cycle feedback also disappeared making the modern carbon cycle critically dependent on the slower silicate weathering feedback.

The occurrence of seep-carbonates associated with shallow gas hydrates is increasingly documented in modern continental margins but in fossil sediments the recognition of gas hydrates is still challenging for the lack of unequivocal proxies. Here, we combined multiple field and geochemical indicators for paleo-gas hydrate occurrence based on present-day analogues to investigate fossil seeps located in the northern Apennines. We recognized clathrite-like structures such as thin-layered, spongy and vuggy textures and microbreccias. Non-gravitational cementation fabrics and pinch-out terminations in cavities within the seep-carbonate deposits are ascribed to irregularly oriented dissociation of gas hydrates. Additional evidences for paleo-gas hydrates are provided by the large dimensions of seep-carbonate masses and by the association with sedimentary instability in the host sediments. We report heavy oxygen isotopic values in the examined seep-carbonates up to +6‰ that are indicative of a contribution of isotopically heavier fluids released by gas hydrate decomposition. The calculation of the stability field of methane hydrates for the northern Apennine wedge-foredeep system during the Miocene indicated the potential occurrence of shallow gas hydrates in the upper few tens of meters of sedimentary column.