Implementing permissive dehydration (DEH) during short-term heat acclimation (HA) may accelerate adaptations to the heat. However, HA with DEH may augment risk for acute kidney injury (AKI). This study investigated the effect of HA with permissive DEH on time-trial performance and markers of AKI. Fourteen moderately trained men (age and VO_2max = 25 ± 0.5 yr and 51.6 ± 1.8 mL^.kg^−1.min⁻¹) were randomly assigned to DEH or euhydration (EUH). Time-trial performance and VO_2max were assessed in a temperate environment before and after 7 d of HA. Heat acclimation consisted of 90 min of cycling in an environmental chamber (40 °C, 35% RH). Neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) were assessed pre- and post-exercise on day 1 and day 7 of HA. Following HA, VO_2max did not change in either group (p = 0.099); however, time-trial performance significantly improved (3%, p < 0.01) with no difference between groups (p = 0.485). Compared to pre-exercise, NGAL was not significantly different following day 1 and 7 of HA (p = 0.113) with no difference between groups (p = 0.667). There was a significant increase in KIM-1 following day 1 and 7 of HA (p = 0.002) with no difference between groups (p = 0.307). Heat acclimation paired with permissive DEH does not amplify improvements in VO_2max or time-trial performance in a temperate environment versus EUH and does not increase markers of AKI.

The aim of the present study was to assess the validity of verification phase (VP) testing and a 3 min all-out test to determine critical power (CP) in males with obesity. Nine young adult males with a body mass index (BMI) ≥ 30 kg·m⁻² completed a cycle ergometer ramp-style VO_2max test, four randomized VP tests at 80, 90, 100, and 105% of maximum wattage attained during the ramp test, and a 3 min all-out test. There was a significant main effect for VO_2max across all five tests (p = 0.049). Individually, 8 of 9 participants attained a higher VO_2max (L/min) during a VP test compared to the ramp test. A trend (p = 0.06) was observed for VO_2max during the 90% VP test (3.61 ± 0.54 L/min) when compared to the ramp test (3.37 ± 0.39 L/min). A significantly higher VO_2max (p = 0.016) was found in the VP tests that occurred below 130% of CP wattage (N = 15, VO_2max = 3.76 ± 0.52 L/min) compared to those that were above (N = 21, VO_2max = 3.36 ± 0.41 L/min). Our findings suggest submaximal VP tests at 90% may elicit the highest VO_2max in males with obesity and there may be merit in using % of CP wattage to determine optimal VP intensity.