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Domenico Galante
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy

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Journal article
Published: 02 June 2021 in Microorganisms
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Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry (MALDI-TOF MS) technology is currently increasingly used in diagnostic laboratories as a cost effective, rapid and reliable routine technique for the identification and typing of microorganisms. In this study, we used MALDI-TOF MS to analyze a collection of 160 strains belonging to the Bacillus cereus group (57 B. anthracis, 49 B. cereus, 1 B. mycoides, 18 B. wiedmannii, 27 B. thuringiensis, 7 B. toyonensis and 1 B. weihenstephanensis) and to detect specific biomarkers which would allow an unequivocal identification. The Main Spectra Profiles (MSPs) were added to an in-house reference library, expanding the current commercial library which does not include B. toyonensis and B. wiedmannii mass spectra. The obtained mass spectra were statistically compared by Principal Component Analysis (PCA) that revealed seven different clusters. Moreover, for the identification purpose, were generated dedicate algorithms for a rapid and automatic detection of characteristic ion peaks after the mass spectra acquisition. The presence of specific biomarkers can be used to differentiate strains within the B. cereus group and to make a reliable identification of Bacillus anthracis, etiologic agent of anthrax, which is the most pathogenic and feared bacterium of the group. This could offer a critical time advantage for the diagnosis and for the clinical management of human anthrax even in case of bioterror attacks.

ACS Style

Viviana Manzulli; Valeria Rondinone; Alessandro Buchicchio; Luigina Serrecchia; Dora Cipolletta; Antonio Fasanella; Antonio Parisi; Laura Difato; Michela Iatarola; Angela Aceti; Elena Poppa; Francesco Tolve; Lorenzo Pace; Fiorenza Petruzzi; Ines Rovere; Donato Raele; Laura Del Sambro; Luigi Giangrossi; Domenico Galante. Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry. Microorganisms 2021, 9, 1202 .

AMA Style

Viviana Manzulli, Valeria Rondinone, Alessandro Buchicchio, Luigina Serrecchia, Dora Cipolletta, Antonio Fasanella, Antonio Parisi, Laura Difato, Michela Iatarola, Angela Aceti, Elena Poppa, Francesco Tolve, Lorenzo Pace, Fiorenza Petruzzi, Ines Rovere, Donato Raele, Laura Del Sambro, Luigi Giangrossi, Domenico Galante. Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry. Microorganisms. 2021; 9 (6):1202.

Chicago/Turabian Style

Viviana Manzulli; Valeria Rondinone; Alessandro Buchicchio; Luigina Serrecchia; Dora Cipolletta; Antonio Fasanella; Antonio Parisi; Laura Difato; Michela Iatarola; Angela Aceti; Elena Poppa; Francesco Tolve; Lorenzo Pace; Fiorenza Petruzzi; Ines Rovere; Donato Raele; Laura Del Sambro; Luigi Giangrossi; Domenico Galante. 2021. "Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry." Microorganisms 9, no. 6: 1202.

Journal article
Published: 22 April 2021 in Viruses
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In order to provide insights into the evolutionary and epidemiological viral dynamics during the current COVID-19 pandemic in South Eastern Italy, a total of 298 genomes of SARS-CoV-2 strains collected in the Apulia and Basilicata regions, between March 2020 and January 2021, were sequenced. The genomic analysis performed on the draft genomes allowed us to assign the genetic clades and lineages of belonging to each sample and provide an overview of the main circulating viral variants. Our data showed the spread in Apulia and Basilicata of SARS-CoV-2 variants which have emerged during the second wave of infections and are being currently monitored worldwide for their increased transmission rate and their possible impact on vaccines and therapies. These results emphasize the importance of genome sequencing for the epidemiological surveillance of the new SARS-CoV-2 variants’ spread.

ACS Style

Loredana Capozzi; Angelica Bianco; Laura Del Sambro; Domenico Simone; Antonio Lippolis; Maria Notarnicola; Graziano Pesole; Lorenzo Pace; Domenico Galante; Antonio Parisi. Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study. Viruses 2021, 13, 731 .

AMA Style

Loredana Capozzi, Angelica Bianco, Laura Del Sambro, Domenico Simone, Antonio Lippolis, Maria Notarnicola, Graziano Pesole, Lorenzo Pace, Domenico Galante, Antonio Parisi. Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study. Viruses. 2021; 13 (5):731.

Chicago/Turabian Style

Loredana Capozzi; Angelica Bianco; Laura Del Sambro; Domenico Simone; Antonio Lippolis; Maria Notarnicola; Graziano Pesole; Lorenzo Pace; Domenico Galante; Antonio Parisi. 2021. "Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study." Viruses 13, no. 5: 731.

Journal article
Published: 15 April 2021 in Pathogens
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In 2011, in Bangladesh, 11 anthrax outbreaks occurred in six districts of the country. Different types of samples were collected from May to September in the six districts where anthrax had occurred in order to detect and type Bacillus anthracis (B. anthracis) strains. Anthrax was detected in 46.6% of the samples analysed, in particular in soils, but also in bone samples, water, animal feed, and rumen ingesta of dead animals. Canonical single nucleotide polymorphisms (CanSNPs) analysis showed that all the isolates belonged to the major lineage A, sublineage A.Br.001/002 of China and Southeast Asia while the multi-locus variable number of tandem repeats (VNTRs) analysis (MLVA) with 15 VNTRs demonstrated the presence of five genotypes, of which two resulted to be new genotypes. The single nucleotide repeats (SNRs) analysis showed 13 SNR types; nevertheless, due to its higher discriminatory power, the presence of two isolates with different SNR-type polymorphisms was detected within two MLVA genotypes. This study assumes that soil is not the only reason for the spread of the disease in Bangladesh; contaminated feed and water can also play an important role in the epidemiology of anthrax. Possible explanations for these epidemiological relationships are discussed.

ACS Style

Domenico Galante; Viviana Manzulli; Luigina Serrecchia; Pietro Taranto; Martin Hugh-Jones; M. Hossain; Valeria Rondinone; Dora Cipolletta; Lorenzo Pace; Michela Iatarola; Francesco Tolve; Angela Aceti; Elena Poppa; Antonio Fasanella. Investigation on Anthrax in Bangladesh during the Outbreaks of 2011 and Definition of the Epidemiological Correlations. Pathogens 2021, 10, 481 .

AMA Style

Domenico Galante, Viviana Manzulli, Luigina Serrecchia, Pietro Taranto, Martin Hugh-Jones, M. Hossain, Valeria Rondinone, Dora Cipolletta, Lorenzo Pace, Michela Iatarola, Francesco Tolve, Angela Aceti, Elena Poppa, Antonio Fasanella. Investigation on Anthrax in Bangladesh during the Outbreaks of 2011 and Definition of the Epidemiological Correlations. Pathogens. 2021; 10 (4):481.

Chicago/Turabian Style

Domenico Galante; Viviana Manzulli; Luigina Serrecchia; Pietro Taranto; Martin Hugh-Jones; M. Hossain; Valeria Rondinone; Dora Cipolletta; Lorenzo Pace; Michela Iatarola; Francesco Tolve; Angela Aceti; Elena Poppa; Antonio Fasanella. 2021. "Investigation on Anthrax in Bangladesh during the Outbreaks of 2011 and Definition of the Epidemiological Correlations." Pathogens 10, no. 4: 481.

Journal article
Published: 02 April 2021 in Water Research
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New SARS-CoV-2 mutations are constantly emerging, raising concerns of increased transmissibility, virulence or escape from host immune response. We describe a nested RT-PCR assay (~1500 bps) to detect multiple nucleotide changes resulting in key spike protein mutations distinctive of the major known circulating SARS-CoV-2 variants, including the three Variants of Concern (VOCs) 20I/501Y.V1 (United Kingdom), 20H/501Y.V2 (South Africa), and 20 J/501Y.V3 (Brazil), as well as the 20E.EU1 variant (Spain), the CAL.20C recently identified in California, and the mink-associated variant (GR, lineage B.1.1.298). Prior to application to field samples, the discriminatory potential of this PCR assay was explored using GISAID and Nextclade. To extend variant detection to challenging matrices such as sewage, where the amplification of long fragments is problematic, two short nested RT-PCR assays (~300 bps) were also designed, targeting portions of the region spanned by the long nested assay. The three newly-designed assays were then tested on field samples, including 31 clinical samples (7 fully-sequenced swab samples, and 24 uncharacterized ones) and 34 urban wastewater samples, some of which collected in areas where circulation of VOCs had been reported. The long assay successfully amplified 29 of the 31 swabs (93%), allowing the correct identification of variants 20I/501Y.V1 and 20E.EU1 present in the panel of previously characterized samples. The Spanish variant was detected in 14/24 of the uncharacterized samples as well. The sequences obtained using the short assays were consistent with those obtained with the long assay. Mutations characteristic of VOCs (UK and Brazilian variant) and of other variant (Spanish) were detected in sewage samples. To our knowledge, this is the first evidence of the presence of sequences harboring key mutations of 20I/501Y.V1 and 20 J/501Y.V3 in urban wastewaters, highlighting the potential contribution of wastewater surveillance to explore SARS-CoV-2 diversity. The developed nested RT-PCR assays can be used as an initial rapid screening test to select clinical samples containing mutations of interest. This can speed up diagnosis and optimize resources since it allows full genome sequencing to be done only on clinically relevant specimens. The assays can be also employed for a rapid and cost-effective detection of VOCs or other variants in sewage for the purposes of wastewater-based epidemiology. The approach proposed here can be used to better understand SARS-CoV-2 variant diversity, geographic distribution and impact worldwide.

ACS Style

G La Rosa; P. Mancini; G. Bonanno Ferraro; C. Veneri; M. Iaconelli; L. Lucentini; L. Bonadonna; S. Brusaferro; D. Brandtner; A. Fasanella; L. Pace; A. Parisi; D. Galante; E. Suffredini. Rapid screening for SARS-CoV-2 variants of concern in clinical and environmental samples using nested RT-PCR assays targeting key mutations of the spike protein. Water Research 2021, 197, 117104 -117104.

AMA Style

G La Rosa, P. Mancini, G. Bonanno Ferraro, C. Veneri, M. Iaconelli, L. Lucentini, L. Bonadonna, S. Brusaferro, D. Brandtner, A. Fasanella, L. Pace, A. Parisi, D. Galante, E. Suffredini. Rapid screening for SARS-CoV-2 variants of concern in clinical and environmental samples using nested RT-PCR assays targeting key mutations of the spike protein. Water Research. 2021; 197 ():117104-117104.

Chicago/Turabian Style

G La Rosa; P. Mancini; G. Bonanno Ferraro; C. Veneri; M. Iaconelli; L. Lucentini; L. Bonadonna; S. Brusaferro; D. Brandtner; A. Fasanella; L. Pace; A. Parisi; D. Galante; E. Suffredini. 2021. "Rapid screening for SARS-CoV-2 variants of concern in clinical and environmental samples using nested RT-PCR assays targeting key mutations of the spike protein." Water Research 197, no. : 117104-117104.

Communication
Published: 11 February 2021 in Viruses
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The coronavirus disease 2019 (Covid-19) pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and presents a global health emergency that needs urgent intervention. Viruses constantly change through mutation, and new variants of a virus are expected to occur over time. In the United Kingdom (UK), a new variant called B.1.1.7 has emerged with an unusually large number of mutations. The aim of this study is to evaluate the level of protection of sera from 12 patients infected and later healed in Apulia Region (Italy) with Covid-19 between March and November 2020, when the English variant was not circulating in this territory yet, against the new VOC 202012/01 variant by seroneutralization assay. The sera of patients had already been tested before, using a virus belonging to the lineage B.1 and showed an antibody neutralizing titer ranging between 1:160 and 1:320. All the 12 sera donors confirmed the same titers of neutralizing antibodies obtained with a strain belonging to the lineage B.1.1.7 (VOC 202012/01). These data indicate that antibodies produced in subjects infected with variants of Sars-CoV-2 strain before the appearance of the English one, seem to have a neutralizing power also against this variant.

ACS Style

Valeria Rondinone; Lorenzo Pace; Antonio Fasanella; Viviana Manzulli; Antonio Parisi; Maria Capobianchi; Angelo Ostuni; Maria Chironna; Elisabetta Caprioli; Maria Labonia; Dora Cipolletta; Ines Della Rovere; Luigina Serrecchia; Fiorenza Petruzzi; Germana Pennuzzi; Domenico Galante. VOC 202012/01 Variant Is Effectively Neutralized by Antibodies Produced by Patients Infected before Its Diffusion in Italy. Viruses 2021, 13, 276 .

AMA Style

Valeria Rondinone, Lorenzo Pace, Antonio Fasanella, Viviana Manzulli, Antonio Parisi, Maria Capobianchi, Angelo Ostuni, Maria Chironna, Elisabetta Caprioli, Maria Labonia, Dora Cipolletta, Ines Della Rovere, Luigina Serrecchia, Fiorenza Petruzzi, Germana Pennuzzi, Domenico Galante. VOC 202012/01 Variant Is Effectively Neutralized by Antibodies Produced by Patients Infected before Its Diffusion in Italy. Viruses. 2021; 13 (2):276.

Chicago/Turabian Style

Valeria Rondinone; Lorenzo Pace; Antonio Fasanella; Viviana Manzulli; Antonio Parisi; Maria Capobianchi; Angelo Ostuni; Maria Chironna; Elisabetta Caprioli; Maria Labonia; Dora Cipolletta; Ines Della Rovere; Luigina Serrecchia; Fiorenza Petruzzi; Germana Pennuzzi; Domenico Galante. 2021. "VOC 202012/01 Variant Is Effectively Neutralized by Antibodies Produced by Patients Infected before Its Diffusion in Italy." Viruses 13, no. 2: 276.

Journal article
Published: 15 January 2021 in Journal of Clinical Medicine
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Background. The highly variable manifestation of the COVID-19 disease, from completely asymptomatic to fatal, is both a clinical and a public health issue. The criteria for discharge of hospitalized patients have been based so far on the negative result of Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) tests, but the persistence of viral fragments may exceed that of the integral virus by weeks. The aim of our study was to verify the clearance of the virus at viral culture in patients hospitalized for COVID-19 that have clinically recovered but are still positive on nasopharyngeal swab. Methods. The study was conducted in hospitalized patients with positive RT-PCR on nasopharyngeal swab. Patients included were from asymptomatic to severe cases and performed nasopharyngeal control swabbing on day 14 for asymptomatic patient or at least three days after remission of symptoms. RT-PCR positive specimens were sent to a biosafety level 3 laboratory for viral culture. Results. We performed a combined analysis of RT-PCR and a highly sensitive in vitro culture from 84 samples of hospitalized patients. The average age was 46 ± 20.29, and 40.5% of the subjects had radiologically confirmed pneumonia, with average PaO2 of 72.35 ± 12.12and P/F ratio of 315 ± 83.15. Ct values for the N gene were lower in the first swab than in the control one (p < 0.001). The samples from 83 patients were negative at viral culture, and RT-PCR on the respective supernatants always confirmed the absence of viral growth. Conclusions. Our preliminary results demonstrate that patients clinically recovered for at least three days show the viral clearance at viral culture, and presumably they continued to not be contagious.

ACS Style

Viviana Manzulli; Giulia Scioscia; Giulio Giganti; Maria Rosaria Capobianchi; Donato Lacedonia; Lorenzo Pace; Dora Cipolletta; Pasquale Tondo; Rosella De Nittis; Valeria Rondinone; Luigina Serrecchia; Antonio Parisi; Domenico Galante; Sergio Lo Caputo; Teresa Antonia Santantonio; Damiana Moschetta; Vitangelo Dattoli; Antonio Fasanella; Maria Pia Foschino Barbaro. Real Time PCR and Culture-Based Virus Isolation Test in Clinically Recovered Patients: Is the Subject Still Infectious for SARS-CoV2? Journal of Clinical Medicine 2021, 10, 309 .

AMA Style

Viviana Manzulli, Giulia Scioscia, Giulio Giganti, Maria Rosaria Capobianchi, Donato Lacedonia, Lorenzo Pace, Dora Cipolletta, Pasquale Tondo, Rosella De Nittis, Valeria Rondinone, Luigina Serrecchia, Antonio Parisi, Domenico Galante, Sergio Lo Caputo, Teresa Antonia Santantonio, Damiana Moschetta, Vitangelo Dattoli, Antonio Fasanella, Maria Pia Foschino Barbaro. Real Time PCR and Culture-Based Virus Isolation Test in Clinically Recovered Patients: Is the Subject Still Infectious for SARS-CoV2? Journal of Clinical Medicine. 2021; 10 (2):309.

Chicago/Turabian Style

Viviana Manzulli; Giulia Scioscia; Giulio Giganti; Maria Rosaria Capobianchi; Donato Lacedonia; Lorenzo Pace; Dora Cipolletta; Pasquale Tondo; Rosella De Nittis; Valeria Rondinone; Luigina Serrecchia; Antonio Parisi; Domenico Galante; Sergio Lo Caputo; Teresa Antonia Santantonio; Damiana Moschetta; Vitangelo Dattoli; Antonio Fasanella; Maria Pia Foschino Barbaro. 2021. "Real Time PCR and Culture-Based Virus Isolation Test in Clinically Recovered Patients: Is the Subject Still Infectious for SARS-CoV2?" Journal of Clinical Medicine 10, no. 2: 309.

Case report
Published: 17 September 2020 in IDCases
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This report describes the evolution of COVID-19 in a 10 day-old-baby. The mother developed the disease immediately after childbirth and therefore a vertical transmission can be excluded. The isolation of the virus in cell culture with a cytopathic effect already visible after 48 h, indicates that the viral load of the newborn was quite high, but not serious course of the disease was observed. This paper wants to highlight the possible role of newborns and children in the spread of the disease.

ACS Style

Giuseppe Lenoci; Domenico Galante; Edmondo Ceci; Viviana Manzulli; Angela Maria Moramarco; Anna Chiaromonte; Giuseppina Labarile; Simone Lattarulo; Annalisa Resta; Lorenzo Pace; Valeria Rondinone; Antonio Parisi; Dora Cipolletta; Leonardo Marino; Iolanda Padalino; Luigina Serrecchia; Angela Aceti; Michela Iatarola; Francesco Tolve; Antonio Fasanella. Sars-CoV-2 isolation from a 10-day-old newborn in Italy: A case report. IDCases 2020, 22, e00960 -e00960.

AMA Style

Giuseppe Lenoci, Domenico Galante, Edmondo Ceci, Viviana Manzulli, Angela Maria Moramarco, Anna Chiaromonte, Giuseppina Labarile, Simone Lattarulo, Annalisa Resta, Lorenzo Pace, Valeria Rondinone, Antonio Parisi, Dora Cipolletta, Leonardo Marino, Iolanda Padalino, Luigina Serrecchia, Angela Aceti, Michela Iatarola, Francesco Tolve, Antonio Fasanella. Sars-CoV-2 isolation from a 10-day-old newborn in Italy: A case report. IDCases. 2020; 22 ():e00960-e00960.

Chicago/Turabian Style

Giuseppe Lenoci; Domenico Galante; Edmondo Ceci; Viviana Manzulli; Angela Maria Moramarco; Anna Chiaromonte; Giuseppina Labarile; Simone Lattarulo; Annalisa Resta; Lorenzo Pace; Valeria Rondinone; Antonio Parisi; Dora Cipolletta; Leonardo Marino; Iolanda Padalino; Luigina Serrecchia; Angela Aceti; Michela Iatarola; Francesco Tolve; Antonio Fasanella. 2020. "Sars-CoV-2 isolation from a 10-day-old newborn in Italy: A case report." IDCases 22, no. : e00960-e00960.

Journal article
Published: 09 April 2020 in Research in Veterinary Science
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Astroviruses (AstVs) have been identified in the stools of calves with enteritis and in the brain tissues of bovines with encephalitis but their pathogenic role has not been clarified. In this study, we report the detection and characterization of bovine and water buffalo AstV strains identified in young bovine and buffalo calves with enteritis in Italy between 2012 and 2015. By negative staining transmission electron microscopy (TEM) observation, AstV-like particles were identified in the stools of the animals and AstV RNA was confirmed molecularly. The sequence (~3.2-kb) at the 3′ end of the genome was determined for two bovine and two buffalo AstVs. Sequence and phylogenetic analysis on the partial ORF1b and full-length ORF2 revealed a marked genetic diversity although the viruses were distantly related to other AstV identified from ruminants. Gathering sequence information on ruminant AstVs is important to understand the extent of inter-species circulation and for the development of reliable, specific diagnostic tools.

ACS Style

Vito Martella; Cristiana Catella; Paolo Capozza; Georgia Diakoudi; Michele Camero; Gianvito Lanave; Domenico Galante; Maria Assunta Cafiero; Antonio Lavazza; Krisztián Bányai; Canio Buonavoglia. Identification of astroviruses in bovine and buffalo calves with enteritis. Research in Veterinary Science 2020, 131, 59 -68.

AMA Style

Vito Martella, Cristiana Catella, Paolo Capozza, Georgia Diakoudi, Michele Camero, Gianvito Lanave, Domenico Galante, Maria Assunta Cafiero, Antonio Lavazza, Krisztián Bányai, Canio Buonavoglia. Identification of astroviruses in bovine and buffalo calves with enteritis. Research in Veterinary Science. 2020; 131 ():59-68.

Chicago/Turabian Style

Vito Martella; Cristiana Catella; Paolo Capozza; Georgia Diakoudi; Michele Camero; Gianvito Lanave; Domenico Galante; Maria Assunta Cafiero; Antonio Lavazza; Krisztián Bányai; Canio Buonavoglia. 2020. "Identification of astroviruses in bovine and buffalo calves with enteritis." Research in Veterinary Science 131, no. : 59-68.

Research article
Published: 13 January 2020 in PLOS ONE
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In Italy anthrax is an endemic disease, with a few outbreaks occurring almost every year. We surveyed 234 B. anthracis strains from animals (n = 196), humans (n = 3) and the environment (n = 35) isolated during Italian outbreaks in the years 1972–2018. Despite the considerable genetic homogeneity of B. anthracis, the strains were effectively differentiated using canonical single nucleotide polymorphisms (CanSNPs) assay and multiple-locus variable-number tandem repeat analysis (MLVA). The phylogenetic identity was determined through the characterization of 14 CanSNPs. In addition, a subsequent 31-loci MLVA assay was also used to further discriminate B. anthracis genotypes into subgroups. The analysis of 14 CanSNPs allowed for the identification of four main lineages: A.Br.011/009, A.Br.008/011 (respectively belonging to A.Br.008/009 sublineage, also known Trans-Eurasian or TEA group), A.Br.005/006 and B.Br.CNEVA. A.Br.011/009, the most common subgroup of lineage A, is the major genotype of B. anthracis in Italy. The MLVA analysis revealed the presence of 55 different genotypes in Italy. Most of the genotypes are genetically very similar, supporting the hypothesis that all strains evolved from a local common ancestral strain, except for two genotypes representing the branch A.Br.005/006 and B.Br.CNEVA. The genotyping analysis applied in this study remains a very valuable tool for studying the diversity, evolution, and molecular epidemiology of B. anthracis.

ACS Style

Valeria Rondinone; Luigina Serrecchia; Antonio Parisi; Antonio Fasanella; Viviana Manzulli; Dora Cipolletta; Domenico Galante. Genetic characterization of Bacillus anthracis strains circulating in Italy from 1972 to 2018. PLOS ONE 2020, 15, e0227875 .

AMA Style

Valeria Rondinone, Luigina Serrecchia, Antonio Parisi, Antonio Fasanella, Viviana Manzulli, Dora Cipolletta, Domenico Galante. Genetic characterization of Bacillus anthracis strains circulating in Italy from 1972 to 2018. PLOS ONE. 2020; 15 (1):e0227875.

Chicago/Turabian Style

Valeria Rondinone; Luigina Serrecchia; Antonio Parisi; Antonio Fasanella; Viviana Manzulli; Dora Cipolletta; Domenico Galante. 2020. "Genetic characterization of Bacillus anthracis strains circulating in Italy from 1972 to 2018." PLOS ONE 15, no. 1: e0227875.

Journal article
Published: 08 January 2020 in Microorganisms
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Anthrax is a serious infectious disease caused by the gram-positive and spore-forming bacterium Bacillus anthracis. In Italy, anthrax is an endemic disease with sporadic cases each year and few outbreaks, especially in Southern Italy. However, new foci have been discovered in zones without previous history of anthrax. During summer 2016, an outbreak of anthrax caused the death of four goats in the Abruzzo region, where the disease had not been reported before. In order to investigate the outbreak, we sequenced one strain and compared it to 19 Italian B. anthracis genomes. Furthermore, we downloaded 71 whole genome sequences representing the global distribution of canonical SNP lineages and used them to verify the phylogenetic positioning. To this end, we analyzed and compared the genome sequences using canonical SNPs and the whole genome SNP-based analysis. Our results demonstrate that the outbreak strain belonged to the Trans-Eurasian (TEA) group A.Br.011/009, which is the predominant clade in Central-Southern Italy. In conclusion, the high genomic relatedness of the Italian TEA strains suggests their evolution from a common ancestor, while the spread is supposedly driven by trade as well as human and transhumance activities. Here, we demonstrated the capabilities of whole genome sequencing (WGS), which can be used as a tool for outbreak analyses and surveillance activities.

ACS Style

Alexandra Chiaverini; Mostafa Y. Abdel-Glil; Jörg Linde; Domenico Galante; Valeria Rondinone; Antonio Fasanella; Cesare Cammà; Nicola D’Alterio; Giuliano Garofolo; Herbert Tomaso. Whole Genome Sequencing for Studying Bacillus anthracis from an Outbreak in the Abruzzo Region of Italy. Microorganisms 2020, 8, 87 .

AMA Style

Alexandra Chiaverini, Mostafa Y. Abdel-Glil, Jörg Linde, Domenico Galante, Valeria Rondinone, Antonio Fasanella, Cesare Cammà, Nicola D’Alterio, Giuliano Garofolo, Herbert Tomaso. Whole Genome Sequencing for Studying Bacillus anthracis from an Outbreak in the Abruzzo Region of Italy. Microorganisms. 2020; 8 (1):87.

Chicago/Turabian Style

Alexandra Chiaverini; Mostafa Y. Abdel-Glil; Jörg Linde; Domenico Galante; Valeria Rondinone; Antonio Fasanella; Cesare Cammà; Nicola D’Alterio; Giuliano Garofolo; Herbert Tomaso. 2020. "Whole Genome Sequencing for Studying Bacillus anthracis from an Outbreak in the Abruzzo Region of Italy." Microorganisms 8, no. 1: 87.

Journal article
Published: 31 December 2019 in Vet. Ital.
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ACS Style

Domenico Galante; Maria Assunta Cafiero; Donato Antonio Raele; Nicola Pugliese; Iolanda Padalino; Nicola Cavaliere; Canio Buonavoglia. Identification and characterization of Orf viruses isolated from sheep and goats in Southern Italy. Vet. Ital. 2019, 55, 347 -353.

AMA Style

Domenico Galante, Maria Assunta Cafiero, Donato Antonio Raele, Nicola Pugliese, Iolanda Padalino, Nicola Cavaliere, Canio Buonavoglia. Identification and characterization of Orf viruses isolated from sheep and goats in Southern Italy. Vet. Ital.. 2019; 55 (4):347-353.

Chicago/Turabian Style

Domenico Galante; Maria Assunta Cafiero; Donato Antonio Raele; Nicola Pugliese; Iolanda Padalino; Nicola Cavaliere; Canio Buonavoglia. 2019. "Identification and characterization of Orf viruses isolated from sheep and goats in Southern Italy." Vet. Ital. 55, no. 4: 347-353.

Journal article
Published: 12 February 2019 in TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES
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ACS Style

Donato Antonio Raele; Nicola Pugliese; Domenico Galante; Maria Assunta Cafiero. Loop-mediated isothermal amplification in the field: reality beyond the potentiality. TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES 2019, 43, 173 -175.

AMA Style

Donato Antonio Raele, Nicola Pugliese, Domenico Galante, Maria Assunta Cafiero. Loop-mediated isothermal amplification in the field: reality beyond the potentiality. TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES. 2019; 43 (1):173-175.

Chicago/Turabian Style

Donato Antonio Raele; Nicola Pugliese; Domenico Galante; Maria Assunta Cafiero. 2019. "Loop-mediated isothermal amplification in the field: reality beyond the potentiality." TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES 43, no. 1: 173-175.

Research letter
Published: 01 February 2019 in Emerging Infectious Diseases
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We report biomolecular evidence of dolphin morbillivirus in 4 wild Eurasian otters (Lutra lutra) from southern Italy; 2 animals showed simultaneous immunohistochemical reactivity against morbilliviral antigen. These cases add further concern and support to the progressively expanding host range of dolphin morbillivirus in the western Mediterranean Sea.

ACS Style

I. Padalino Et Al.; Giovanni Di Guardo; Antonio Carbone; Pasquale Troiano; Antonio Parisi; Domenico Galante; Maria Assunta Cafiero; Marta Caruso; Lucia Palazzo; Laura Guarino; Laura De Riso; Cinzia Centelleghe; Sandro Mazzariol; Antonio Petrella. Dolphin Morbillivirus in Eurasian Otters, Italy. Emerging Infectious Diseases 2019, 25, 372 -374.

AMA Style

I. Padalino Et Al., Giovanni Di Guardo, Antonio Carbone, Pasquale Troiano, Antonio Parisi, Domenico Galante, Maria Assunta Cafiero, Marta Caruso, Lucia Palazzo, Laura Guarino, Laura De Riso, Cinzia Centelleghe, Sandro Mazzariol, Antonio Petrella. Dolphin Morbillivirus in Eurasian Otters, Italy. Emerging Infectious Diseases. 2019; 25 (2):372-374.

Chicago/Turabian Style

I. Padalino Et Al.; Giovanni Di Guardo; Antonio Carbone; Pasquale Troiano; Antonio Parisi; Domenico Galante; Maria Assunta Cafiero; Marta Caruso; Lucia Palazzo; Laura Guarino; Laura De Riso; Cinzia Centelleghe; Sandro Mazzariol; Antonio Petrella. 2019. "Dolphin Morbillivirus in Eurasian Otters, Italy." Emerging Infectious Diseases 25, no. 2: 372-374.

Journal article
Published: 01 January 2019 in Journal of Veterinary Science
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Anthrax, caused by Bacillus anthracis, is a non-contagious infectious disease that affects a wide range of animal species (primarily ruminants) including humans. Due to the often-fatal outcome in humans, quick administration of definitely effective antimicrobials is crucial either as prophylaxis or as a clinical case therapy. In this study, 110 B. anthracis strains, temporally, geographically, and genetically different, isolated during anthrax outbreaks in Italy from 1984 to 2017, were screened using a broth microdilution method to determine their susceptibility to 16 clinically relevant antimicrobial agents. The strains were isolated from various matrices (human, animal, and environmental samples) and were representative of thirty distinct genotypes previously identified by 15-loci multiple-locus variable-number of tandem repeats analysis. The antimicrobials tested were gentamicin, ceftriaxone, streptomycin, penicillin G, clindamycin, chloramphenicol, vancomycin, linezolid, cefotaxime, tetracycline, erythromycin, rifampin, amoxicillin, ciprofloxacin, doxycycline, and trimethoprim. All isolates were susceptible to most of the tested antimicrobials, with the exception of trimethoprim for which all of them showed high minimal inhibitory concentration values. An intermediate level of susceptibility was recorded for ceftriaxone and cefotaxime. Although the Centers for Disease Control and Prevention recommend the use of doxycycline, ciprofloxacin, penicillin G, and amoxicillin for treatment of human cases and for post-exposure prophylaxis to anthrax spores, this study shows a high degree of in vitro susceptibility of B. anthracis to many other antimicrobials, suggesting the possibility of an alternative choice for prophylaxis and therapy.

ACS Style

Viviana Manzulli; Antonio Fasanella; Antonio Parisi; Luigina Serrecchia; Adelia Donatiello; Valeria Rondinone; Marta Caruso; Sabine Zange; Alina Tscherne; Nicola DeCaro; Carmine Pedarra; Domenico Galante. Evaluation of in vitro antimicrobial susceptibility of Bacillus anthracis strains isolated during anthrax outbreaks in Italy from 1984 to 2017. Journal of Veterinary Science 2019, 20, 58 -62.

AMA Style

Viviana Manzulli, Antonio Fasanella, Antonio Parisi, Luigina Serrecchia, Adelia Donatiello, Valeria Rondinone, Marta Caruso, Sabine Zange, Alina Tscherne, Nicola DeCaro, Carmine Pedarra, Domenico Galante. Evaluation of in vitro antimicrobial susceptibility of Bacillus anthracis strains isolated during anthrax outbreaks in Italy from 1984 to 2017. Journal of Veterinary Science. 2019; 20 (1):58-62.

Chicago/Turabian Style

Viviana Manzulli; Antonio Fasanella; Antonio Parisi; Luigina Serrecchia; Adelia Donatiello; Valeria Rondinone; Marta Caruso; Sabine Zange; Alina Tscherne; Nicola DeCaro; Carmine Pedarra; Domenico Galante. 2019. "Evaluation of in vitro antimicrobial susceptibility of Bacillus anthracis strains isolated during anthrax outbreaks in Italy from 1984 to 2017." Journal of Veterinary Science 20, no. 1: 58-62.

Clinical letter
Published: 10 July 2018 in JDDG: Journal der Deutschen Dermatologischen Gesellschaft
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Maria Assunta Cafiero; Enza Viviano; Michele Lomuto; Donato Antonio Raele; Domenico Galante; Elena Castelli. Dermatitis durch mesostigmatische Milben (Dermanyssus gallinae, Ornithonyssus [O.] bacoti, O. bursa, O. sylviarum) in Wohngebieten. JDDG: Journal der Deutschen Dermatologischen Gesellschaft 2018, 16, 904 -906.

AMA Style

Maria Assunta Cafiero, Enza Viviano, Michele Lomuto, Donato Antonio Raele, Domenico Galante, Elena Castelli. Dermatitis durch mesostigmatische Milben (Dermanyssus gallinae, Ornithonyssus [O.] bacoti, O. bursa, O. sylviarum) in Wohngebieten. JDDG: Journal der Deutschen Dermatologischen Gesellschaft. 2018; 16 (7):904-906.

Chicago/Turabian Style

Maria Assunta Cafiero; Enza Viviano; Michele Lomuto; Donato Antonio Raele; Domenico Galante; Elena Castelli. 2018. "Dermatitis durch mesostigmatische Milben (Dermanyssus gallinae, Ornithonyssus [O.] bacoti, O. bursa, O. sylviarum) in Wohngebieten." JDDG: Journal der Deutschen Dermatologischen Gesellschaft 16, no. 7: 904-906.

Clinical letter
Published: 22 June 2018 in JDDG: Journal der Deutschen Dermatologischen Gesellschaft
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Maria Assunta Cafiero; Enza Viviano; Michele Lomuto; Donato Antonio Raele; Domenico Galante; Elena Castelli. Dermatitis due to Mesostigmatic mites (Dermanyssus gallinae, Ornithonyssus [O.] bacoti, O. bursa, O. sylviarum ) in residential settings. JDDG: Journal der Deutschen Dermatologischen Gesellschaft 2018, 16, 904 -906.

AMA Style

Maria Assunta Cafiero, Enza Viviano, Michele Lomuto, Donato Antonio Raele, Domenico Galante, Elena Castelli. Dermatitis due to Mesostigmatic mites (Dermanyssus gallinae, Ornithonyssus [O.] bacoti, O. bursa, O. sylviarum ) in residential settings. JDDG: Journal der Deutschen Dermatologischen Gesellschaft. 2018; 16 (7):904-906.

Chicago/Turabian Style

Maria Assunta Cafiero; Enza Viviano; Michele Lomuto; Donato Antonio Raele; Domenico Galante; Elena Castelli. 2018. "Dermatitis due to Mesostigmatic mites (Dermanyssus gallinae, Ornithonyssus [O.] bacoti, O. bursa, O. sylviarum ) in residential settings." JDDG: Journal der Deutschen Dermatologischen Gesellschaft 16, no. 7: 904-906.

Journal article
Published: 22 February 2018 in New Microbes and New Infections
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The poultry red mite (PRM), Dermanyssus gallinae, is a nonburrowing haematophagous nest-dwelling ectoparasite of birds; occasionally it bites humans, inducing dermatitis. The possibility that this parasite may also be involved in transmission of pathogens is an additional concern. We investigated the presence of zoonotic agents in PRMs from bird nests and pets, and related them to urban outbreaks of dermatitis. A total of 98 PRMs from 12 outbreaks of PRM dermatitis that occurred in Italian cities from 2001 to 2017 were molecularly investigated for detection of Coxiella spp. (16S rRNA), Chlamydophila spp. (16S rRNA), Rickettsia spp. (17 kDa protein-encoding gene), Borrelia burgdorferi sensu lato (groEL gene) and Bartonella spp. (16S–23S rRNA intergenic spacer). Of the 12 tested mite pools, one was positive for Coxiella burnetii (100% identity) and two for B. burgdorferi sensu lato (99% with Borrelia afzelii). For the first time, the presence of B. burgdorferi sensu lato and C. burnetii is reported in PRMs from urban areas. Birds, mainly pigeons, can harbour both pathogens. Therefore, birds and their nest-dwelling PRMs may play a role in the epidemiology of these infections.

ACS Style

D.A. Raele; D. Galante; N. Pugliese; G. La Salandra; M. Lomuto; M. Assunta Cafiero. First report of Coxiella burnetii and Borrelia burgdorferi sensu lato in poultry red mites, Dermanyssus gallinae (Mesostigmata, Acari), related to urban outbreaks of dermatitis in Italy. New Microbes and New Infections 2018, 23, 103 -109.

AMA Style

D.A. Raele, D. Galante, N. Pugliese, G. La Salandra, M. Lomuto, M. Assunta Cafiero. First report of Coxiella burnetii and Borrelia burgdorferi sensu lato in poultry red mites, Dermanyssus gallinae (Mesostigmata, Acari), related to urban outbreaks of dermatitis in Italy. New Microbes and New Infections. 2018; 23 ():103-109.

Chicago/Turabian Style

D.A. Raele; D. Galante; N. Pugliese; G. La Salandra; M. Lomuto; M. Assunta Cafiero. 2018. "First report of Coxiella burnetii and Borrelia burgdorferi sensu lato in poultry red mites, Dermanyssus gallinae (Mesostigmata, Acari), related to urban outbreaks of dermatitis in Italy." New Microbes and New Infections 23, no. : 103-109.

Original research
Published: 18 October 2017 in MicrobiologyOpen
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Ixodidae ticks are vectors and reservoirs of several species of rickettsiae, and tick‐borne rickettsioses are reported worldwide. This study was aimed to verify the distribution of spotted fever group rickettsiae associated with ticks in a wild environment, the National Park of Gargano, where there is proximity between wild and domestic animals, and which is within an endemic area for rickettsiosis. Ticks were collected from animals or vegetation, morphologically identified and tested by a PCR targeting the 17kDa gene, and by a loop‐mediated isothermal amplification (LAMP) targeting ompB gene. Out of 34 tested tick pools, 2 from Dermacentor marginatus, 1 from Ixodes ricinus, and 1 from Rhipicephalus turanicus resulted positive. Nucleotide sequences of amplicons showed high similarity with sequences from Rickettsia slovaca, Rickettsia raoultii, Rickettsia helvetica, and Rickettsia felis. The overall calculated infection rate was 26.19 per 1,000, while it rose up to 107.77 when only D. marginatus was considered. The results highlight the association among Ri. slovaca, Ri. raoultii, D. marginatus and wild boars from which infected ticks were collected. Finally, the study shows the low efficacy of the previously described LAMP method for the detection of Rickettsia spp., when compared to PCR, making urgent the development of most effective LAMP protocols.

ACS Style

Donato Antonio Raele; Domenico Galante; Nicola Pugliese; Giovanna La Salandra; Maria Assunta Cafiero. Spotted fever group rickettsiae associated with ixodid ticks in wild environment in Southern Italy. MicrobiologyOpen 2017, 7, e00527 .

AMA Style

Donato Antonio Raele, Domenico Galante, Nicola Pugliese, Giovanna La Salandra, Maria Assunta Cafiero. Spotted fever group rickettsiae associated with ixodid ticks in wild environment in Southern Italy. MicrobiologyOpen. 2017; 7 (1):e00527.

Chicago/Turabian Style

Donato Antonio Raele; Domenico Galante; Nicola Pugliese; Giovanna La Salandra; Maria Assunta Cafiero. 2017. "Spotted fever group rickettsiae associated with ixodid ticks in wild environment in Southern Italy." MicrobiologyOpen 7, no. 1: e00527.

Case reports
Published: 02 August 2017 in Journal of Small Animal Practice
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D. A. Raele; D. Galante; Nicola Pugliese; M. A. Cafiero. Traumatic cutaneous myiasis by Wohlfahrtia magnifica in a stray dog in Italy. Journal of Small Animal Practice 2017, 58, 539 -539.

AMA Style

D. A. Raele, D. Galante, Nicola Pugliese, M. A. Cafiero. Traumatic cutaneous myiasis by Wohlfahrtia magnifica in a stray dog in Italy. Journal of Small Animal Practice. 2017; 58 (9):539-539.

Chicago/Turabian Style

D. A. Raele; D. Galante; Nicola Pugliese; M. A. Cafiero. 2017. "Traumatic cutaneous myiasis by Wohlfahrtia magnifica in a stray dog in Italy." Journal of Small Animal Practice 58, no. 9: 539-539.

Journal article
Published: 01 January 2017 in Journal of Clinical Case Reports
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Avian mite dermatitis is a skin affliction of mammals, including humans caused by bites of nidicoulous, haematophagous mites in the suborder Mesostigmata, which naturally parasitize birds. The red-mite, Dermanyssus gallinae is the most common species implicated in episodes of dermatitis in city-dwellers, worldwide. Symptoms manifest in the form of pruritic, erythematous papules on exposed/covered body areas. We report 20 urban outbreaks of red-mite dermatitis occurring in Southern Italy from 2001 to 2017 (June) and diagnosed through parasitological identification by veterinarian entomologists. The patients, a total of 54 subjects, were infested in their homes/ workplaces by both mites emigrating indoors from deserted nests of sinantropic birds close to the infested edifices and from pet canaries. Red-mites may be the explanation of cases of pruriginous dermatitis of obscure origin in citydwellers. The applying of the One Health approach is crucial for diagnosis, treatment and prevention of dermatitis by epizoonotic ectoparasites.

ACS Style

Domenico Galante; Donato Antonio Raele; Nardella Mc; Piccirilli E; Maria Assunta Cafiero; Michele Lomuto. Outbreaks of Dermanyssus gallinae (Acari, Mesostigmata) Related Dermatitis in Humans in Public and Private Residences, in Italy (2001-2017): An Expanding Skin Affliction. Journal of Clinical Case Reports 2017, 7, 1 .

AMA Style

Domenico Galante, Donato Antonio Raele, Nardella Mc, Piccirilli E, Maria Assunta Cafiero, Michele Lomuto. Outbreaks of Dermanyssus gallinae (Acari, Mesostigmata) Related Dermatitis in Humans in Public and Private Residences, in Italy (2001-2017): An Expanding Skin Affliction. Journal of Clinical Case Reports. 2017; 7 (10):1.

Chicago/Turabian Style

Domenico Galante; Donato Antonio Raele; Nardella Mc; Piccirilli E; Maria Assunta Cafiero; Michele Lomuto. 2017. "Outbreaks of Dermanyssus gallinae (Acari, Mesostigmata) Related Dermatitis in Humans in Public and Private Residences, in Italy (2001-2017): An Expanding Skin Affliction." Journal of Clinical Case Reports 7, no. 10: 1.