Microcystins (MCs) are common cyanobacterial toxins that occur in freshwaters worldwide. Only two of the >200 MC variants have been tested for potential toxicity after oral exposure. This paper reports on the toxicity of 10 different MC congeners identified in algal blooms, microcystin-LR (MCLR), MCLA, MCLF, MCLW, MCLY, MCRR, [Asp3]MCRR, [Asp3,Dhb7]MCRR, MCWR, and MCYR after single administrations to BALB/c mice. In a preliminary MCLR dose–response study of 3 to 9 mg/kg doses, ≥5 mg/kg induced clinical changes, increased serum levels of ALT, AST, and GLDH, liver congestion, increased liver/body weight ratios, and reduced serum glucose and total protein. Based on the extent of these effects, the 10 congeners were administered as single 7 mg/kg oral doses and toxicity evaluated. The greatest toxicity was observed with MCLA and MCLR including a high percentage of moribundity. In addition to eliciting effects similar to those listed above for MCLR, MCLA also induced serum alterations indicative of jaundice. MCLY, and MCYR induced changes like those noted with MCLR, but to lesser extents. MCLW and MCLF exhibited some serum and morphological changes associated with hepatic toxicity, while there were few indications of toxicity after exposures to MCRR, [Asp³]MCRR, [Asp³,Dhb⁷]MCRR, or MCWR. These data illustrate a wide spectrum of hepatic effects and different potencies of these MC congeners.

A field-scale validation is summarized comparing the efficacy of commercially available stabilization amendments with the objective of mitigating per- and polyfluoroalkyl substance (PFAS) leaching from aqueous film-forming foam (AFFF)-impacted source zones. The scope of this work included bench-scale testing to evaluate multiple amendments and application concentrations to mitigate PFAS leachability and the execution of field-scale soil mixing in an AFFF-impacted fire-training area with nearly 2.5 years of post-soil mixing monitoring to validate reductions in PFAS leachability. At the bench scale, several amendments were evaluated and the selection of two amendments for field-scale evaluation was informed: FLUORO-SORB Adsorbent (FS) and RemBind (RB). Five ∼28 m³ test pits (approximately 3 m wide by 3 m long by 3 m deep) were mixed at a site using conventional construction equipment. One control test pit (Test Pit 1) included Portland cement (PC) only (5% dry weight basis). The other four test pits (Test Pits 2 through 5) compared 5 and 10% ratios (dry weight basis) of FS and RB (also with PC). Five separate monitoring events included two to three sample cores collected from each test pit for United States Environmental Protection Agency (USEPA) Method 1315 leaching assessment. After 1 year, a mass balance for each test pit was attempted comparing the total PFAS soil mass before, during, and after leach testing. Bench-scale and field-scale data were in good agreement and demonstrated >99% decrease in total PFAS leachability (mass basis; >98% mole basis) as confirmed by the total oxidizable precursor assay, strongly supporting the chemical stabilization of PFAS.

Microcystins are common freshwater cyanobacterial toxins that affect liver function. The toxicities of five microcystin congeners (microcystin-LA (MCLA), MCLR, MCLY, MCRR, and MCYR) commonly observed in harmful algal blooms (HABs) were evaluated in BALB/c mice after a single oral administration of doses ranging from those that were no observed adverse effect levels (NOAELs) to lowest observed adverse effect levels (LOAELs). Animals were monitored for changes in behavior and appearance, and euthanized 24 h after dosing. Test endpoints included clinical changes, necropsy observations, and serum indicators of hepatic toxicity and general homeostasis. Doses were 0.5–7 mg/kg MCLA, 0.5–11 mg/kg MCLR, 1–7 mg/kg MCLY, 7–22 mg/kg MCRR, and 3–11 mg/kg MCYR. MCLA at 3 mg/kg elevated liver/body weight ratio and liver score, ALT, AST, and GLDH, indicating hepatic toxicity, reduced serum glucose and highly elevated total serum bilirubin. MCLR and MCLY induced similar effects with LOAELs of 5 mg/kg, although a greater extent and severity of effects were observed in MCLR animals. MCRR exposure at 22 mg/kg was associated with reduced serum glucose. MCYR induced scattered liver effects at 7 mg/kg and reduced serum glucose levels at 5 mg/kg. The results indicate significant differences in congener-induced toxicity after microcystin exposure.