Fluorescence imaging in near-infrared IIb (NIR-IIb, 1500–1700 nm) spectrum holds a great promise for tissue imaging. While few inorganic NIR-IIb fluorescent probes have been reported, their organic counterparts are still rarely developed, possibly due to the shortage of efficient materials with long emission wavelength. Herein, we propose a molecular design philosophy to explore pure organic NIR-IIb fluorophores by manipulation of the effects of twisted intramolecular charge transfer and aggregation-induced emission at the molecular and morphological levels. An organic fluorescent dye emitting up to 1600 nm with a quantum yield of 11.5% in the NIR-II region is developed. NIR-IIb fluorescence imaging of blood vessels and deeply-located intestinal tract of live mice based on organic dyes is achieved with high clarity and enhanced signal-to-background ratio. We hope this study will inspire further development on the evolution of pure organic NIR-IIb dyes for bio-imaging.

Conventional π-conjugated luminophores suffer from problems such as emission quenching, biotoxicity, environmental pollution, etc. The emerging nonconjugated and nonaromatic clusteroluminogens (CLgens) are expected to overcome these stubborn drawbacks, so research of CLgens shows great significance not only for practical application but also for the construction of fundamental photophysical theories. This perspective summarizes the unusual features of CLgens in comparison to traditional chromophores, such as nonconjugated molecular structures, unmatched absorption and excitation, excitation-dependent luminescence, multiple emission peaks, and room-temperature phosphorescence. Different from the theory of through-bond conjugation in π-conjugated luminophores, through-space interactions, including through-space n···n interaction and through-space n···π interaction, are regarded as the emitting sources of nonconjugated CLgens. In addition, the formation of network clusters is proposed as an efficient strategy to improve the performance of CLgens, and their potential applications of anticounterfeiting, photoelectronic devices, and bioimaging are prospected.