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Background: Citrullus colocynthis L. (C. colocynthis) is commonly known as colocynth. It belongs to the family Cucurbitaceae that is frequently used in alternative medicine in the north of Africa. The aim of the study: the present research was undertaken to investigate the chemical composition, antioxidant, antiproliferative, and antibacterial potentials of C. colocynthis seed extract. Material and methods: the chemical composition of C. colocynthis seed organic extract was characterized using gas chromatography/mass spectrometry (GC-MS). The antioxidant property was carried out using both β-carotene bleaching and DPPH assays. The antibacterial effect was effectuated using the agar disc diffusion method. The antiproliferative activity vs. human colorectal adenocarcinoma cell line (HT-29) and human breast adenocarcinoma cell line (MDA MB 231) were carried by WST-1 test. The chemical analysis showed the presence of interesting potentially bioactive compounds. The studied plant extract exhibited antioxidant potential with IC50 value of 2. 22 mg/mL (β-carotene bleaching) and 8.98 ± 0.619 mg/mL (DPPH). Concerning the antiproliferative activity, the seed extract was effective in MDA-MB-231 and HT-29 cancer cells with IC50 values 86.89 ± 3.395 and 242.1 ± 17.9 μg/mL, respectively, whilst the extract of Citrullus colocynthis seeds was non-toxic in healthy human dermal fibroblasts. Regarding the antibacterial test, the extract was effective in Gram-positive bacteria only. Conclusion: The outcome of this research indicated that the extracts from C. colocynthis seeds may compose a promising source with interesting compounds that can be used to fight cancer, free radicals damage, and bacterial infections.
Mohammed Bourhia; Kaoutar Bouothmany; Hanane Bakrim; Safaa Hadrach; Ahmad Salamatullah; Abdulhakeem Alzahrani; Heba Khalil Alyahya; Nawal Albadr; Said Gmouh; Amine Laglaoui; Mohammed El Mzibri; Laila Benbacer. Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds. Separations 2021, 8, 114 .
AMA StyleMohammed Bourhia, Kaoutar Bouothmany, Hanane Bakrim, Safaa Hadrach, Ahmad Salamatullah, Abdulhakeem Alzahrani, Heba Khalil Alyahya, Nawal Albadr, Said Gmouh, Amine Laglaoui, Mohammed El Mzibri, Laila Benbacer. Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds. Separations. 2021; 8 (8):114.
Chicago/Turabian StyleMohammed Bourhia; Kaoutar Bouothmany; Hanane Bakrim; Safaa Hadrach; Ahmad Salamatullah; Abdulhakeem Alzahrani; Heba Khalil Alyahya; Nawal Albadr; Said Gmouh; Amine Laglaoui; Mohammed El Mzibri; Laila Benbacer. 2021. "Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds." Separations 8, no. 8: 114.
Background: Gramineae damping-off disease is a growing problem worldwide, which affects a large range of seedlings in nurseries, glasshouses, gardens, crops, forests and untimely generates a heavy economic impact on the agriculture and related sectors. Objectives: The present study was conducted to evaluate the preventive potential of carvacrol on germination of Fusarium oxysporum, Neocosmospora solani, and Microdochium nivale spores as responsible agents for Lolium perenne seeds damping-off disease. Material and methods: Macrodilution method in agar medium, spore germination, spore destruction, and preventive treatment bioassays were used to achieve this goal. Results: The minimum inhibitory concentration (MIC) of carvacrol vs. tested strains existed in the range of 0.25–0.5 mg/mL. Carvacrol used in concentrations ranging from 0.2 to 0.4 mg/mL inhibited the germination of all fungal spores in a dose-dependent manner. Carvacrol showed a very strong sporicidal effect against all studied fungal strains, and this effect was well confirmed by microscopic observations. The percentage of growth inhibition was found to be strictly correlated to carvacrol dose up vs. all strains. Carvacrol increased the emergence of L. perenne seeds when compared to both uninfested and infested seeds. Conclusion: Based on the results obtained, carvacrol fulfills the requirement for being a natural alternative agent to fight Gramineae seedlings’ damping-off caused by fungal species without adverse effects on the plants.
Hamza Saghrouchni; Azeddin El Barnossi; Ahmad Salamatullah; Mohammed Bourhia; Abdulhakeem Alzahrani; Mohammed Alkaltham; Heba Alyahya; Nour Tahiri; Hamada Imtara; Işıl Var. Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species. Agronomy 2021, 11, 985 .
AMA StyleHamza Saghrouchni, Azeddin El Barnossi, Ahmad Salamatullah, Mohammed Bourhia, Abdulhakeem Alzahrani, Mohammed Alkaltham, Heba Alyahya, Nour Tahiri, Hamada Imtara, Işıl Var. Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species. Agronomy. 2021; 11 (5):985.
Chicago/Turabian StyleHamza Saghrouchni; Azeddin El Barnossi; Ahmad Salamatullah; Mohammed Bourhia; Abdulhakeem Alzahrani; Mohammed Alkaltham; Heba Alyahya; Nour Tahiri; Hamada Imtara; Işıl Var. 2021. "Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species." Agronomy 11, no. 5: 985.
Camel meat is one of the most consumed meats in Arab countries. The use of natural antimicrobial agents to extend the shelf life of fresh camel meat, control Campylobacter jejuni contamination, and preserve meat quality is preferred. In this study, we determined the antimicrobial effects of using 1% or 2% Citrox alone or in combination with 1% chitosan on the survival of C. jejuni in vitro and on camel meat samples during storage at 4 or 10 °C for 30 days in vacuum packaging. We determined the total viable count (TVC (cfu/g)), total volatile base nitrogen (TVB-N) content, and pH of the treated camel meat samples every three days during storage. The shelf lives of camel meat samples treated with 2% Citrox alone or in combination with 1% chitosan were longer than those of camel meat samples treated with 1% Citrox alone or in combination with 1% chitosan at both the 4 and 10 °C storage temperatures, with TVCs of <100 cfu/g after the first ten days and six days of storage at 4 and 10 °C, respectively. The addition of Citrox (1% and 2%) and 1% chitosan to camel meat samples and the application of vacuum storage were more effective than using Citrox (1% and 2%) alone and led to a reduction in C. jejuni in approximately 4.0 and 3.5 log cycles at 4 and 10 °C, respectively. The experimental results demonstrated that using a Citrox-chitosan combination improved the quality of camel meat and enhanced the long-term preservation of fresh meat for up to or more than 30 days at 4 °C.
Hany Yehia; Abdulrahman Al-Masoud; Manal Elkhadragy; Shereen Korany; Hend Nada; Najla Albaridi; Abdulhakeem Alzahrani; Mosffer Al-Dagal. Improving the Quality and Safety of Fresh Camel Meat Contaminated with Campylobacter jejuni Using Citrox, Chitosan, and Vacuum Packaging to Extend Shelf Life. Animals 2021, 11, 1152 .
AMA StyleHany Yehia, Abdulrahman Al-Masoud, Manal Elkhadragy, Shereen Korany, Hend Nada, Najla Albaridi, Abdulhakeem Alzahrani, Mosffer Al-Dagal. Improving the Quality and Safety of Fresh Camel Meat Contaminated with Campylobacter jejuni Using Citrox, Chitosan, and Vacuum Packaging to Extend Shelf Life. Animals. 2021; 11 (4):1152.
Chicago/Turabian StyleHany Yehia; Abdulrahman Al-Masoud; Manal Elkhadragy; Shereen Korany; Hend Nada; Najla Albaridi; Abdulhakeem Alzahrani; Mosffer Al-Dagal. 2021. "Improving the Quality and Safety of Fresh Camel Meat Contaminated with Campylobacter jejuni Using Citrox, Chitosan, and Vacuum Packaging to Extend Shelf Life." Animals 11, no. 4: 1152.
Fruits and vegetables constitute a considerable amount of antioxidants and among them eggplant is a rich source of polyphenol compounds. This study investigated the bioactive and antimicrobial properties of eggplant under different degree of microwave cooking. The eggplant was cooked for 7 min (light cooked), 10 min (medium cooked), and 15 min (high cooked). The highest total polyphenol content was observed in the light cooked eggplant sample (27.35 mg gallic acid equivalent (GAE)/g dry weight (DW)) followed by high cooked sample (26.10 mg GAE/g DW), while the lowest total polyphenol content (2.79 mg GAE/g DW) was obtained for the uncooked (control) sample. The total polyphenol content of the samples ranged in the following order; light cooked > high cooked > medium cooked > uncooked. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging of eggplant ranged between 16.16% (control) and 47.88% (high cooked). The highest reducing power was exhibited by the light cooked (absorbance 1.708) eggplant sample followed by the high cooked (absorbance 1.597), while the lowest reducing power was shown by uncooked sample (absorbance 0.389). Moreover, antimicrobial studies showed that light cooked eggplant sample demonstrated broad-spectrum inhibition of growth in Gram-positive as well as Gram-negative bacteria and Candida albicans. Slightly lower antimicrobial potential was exhibited by medium cooked eggplant sample while no antibacterial or antifungal activity was recorded for the extract of high cooked eggplant sample. Microwave cooking might be a method to enhance the antioxidant and antimicrobial potential of eggplant.
Ahmad Salamatullah; Mohammed Alkaltham; Khizar Hayat; Mohammed Ahmed; Shaista Arzoo; Fohad Husain; Abdulhakeem Alzahrani. Bioactive and Antimicrobial Properties of Eggplant (Solanum melongena L.) under Microwave Cooking. Sustainability 2021, 13, 1519 .
AMA StyleAhmad Salamatullah, Mohammed Alkaltham, Khizar Hayat, Mohammed Ahmed, Shaista Arzoo, Fohad Husain, Abdulhakeem Alzahrani. Bioactive and Antimicrobial Properties of Eggplant (Solanum melongena L.) under Microwave Cooking. Sustainability. 2021; 13 (3):1519.
Chicago/Turabian StyleAhmad Salamatullah; Mohammed Alkaltham; Khizar Hayat; Mohammed Ahmed; Shaista Arzoo; Fohad Husain; Abdulhakeem Alzahrani. 2021. "Bioactive and Antimicrobial Properties of Eggplant (Solanum melongena L.) under Microwave Cooking." Sustainability 13, no. 3: 1519.