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The present study aims to improve biomass and biochemical constituents, especially lipid production of Anabaena variabilis and Nostoc muscorum by formulating an optimal growth condition using various concentrations of nutrients (NO3−, PO43− and CO32−) for biodiesel production. The supplementation of the three nutrients by +50% showed the maximum dry weight and biomass productivity, while the macromolecule contents were varied. The depletion of N-NO3− by 50% N-NO3− showed the maximum lipid yield (146.67 mg L−1) in A. variabilis and the maximum carbohydrate contents (285.33 mg L−1) in N. muscorum with an increase of 35% and 30% over control of the synthetic medium, respectively. However, variation in P-PO43− and C-CO32− showed insignificant improving results for all biochemical compositions in both cyanobacteria. A. variabilis was the superior species for lipid and protein accumulation; however, N. muscorum showed the maximum carbohydrate content. Accordingly, A. variabilis was selected for biodiesel production. In A. variabilis, −50% N-NO3− resulted in 35% higher lipid productivity compared to the control. Furthermore, the fatty acid profile and biodiesel quality-related parameters have improved under this condition. This study has revealed the strategies to improve A. variabilis lipid productivity for biodiesel production for small-scale in vitro application in terms of fuel quality under low nitrate levels.
Shimaa M. El Shafay; Ahmed Gaber; Walaa F. Alsanie; Mostafa E. Elshobary. Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production. Sustainability 2021, 13, 9081 .
AMA StyleShimaa M. El Shafay, Ahmed Gaber, Walaa F. Alsanie, Mostafa E. Elshobary. Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production. Sustainability. 2021; 13 (16):9081.
Chicago/Turabian StyleShimaa M. El Shafay; Ahmed Gaber; Walaa F. Alsanie; Mostafa E. Elshobary. 2021. "Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production." Sustainability 13, no. 16: 9081.
Bioactive molecules derived from seaweed extracts are revolutionary bio-stimulants used to enhance plant growth and increase yield production. This study evaluated the effectiveness of a commercially available seaweed liquid extract, namely, True-Algae-Max (TAM®), as a plant growth stimulant on nutritional, and antioxidant activity of Capsicum annuum. Three concentrations of TAM® (0.25, 0.5, and 1%) of various NPK: TAM® ratios were investigated via foliar spray, over 2017 and 2018 cultivation seasons, under greenhouse conditions. TAM® is rich in phytochemical compounds, such as ascorbic acid (1.66 mg g−1), phenolics (101.67 mg g−1), and flavonoids (2.60 mg g−1) that showed good antioxidant activity (54.52 mg g−1) and DPPH inhibition of 70.33%. Promoting measured parameter results stated the extensive potentiality of TAM® application, in comparison with conventional NPK treatment. Yield and composition of C. annuum were significantly improved in all TAM® treated groups, especially the TAM0.5% concentration, which resulted in maximum yield (4.23 Kg m−2) and significant amounts of profuse biological molecules like chlorophyll, ascorbic, phenolic compounds, flavonoids, and total nutrients. Compared to the NPK control treatments, C. annuum treated with TAM0.5% improved the total antioxidant activity of hot Pepper from 162.16 to 190.95 mg g−1. These findings indicate that the extract of seaweed can be used as an environmentally friendly, multi-functional biostimulant in the agricultural field for more sustainable production, in addition to reducing the use of hazardous synthetic fertilizers.
Mohamed Ashour; Shimaa Hassan; Mostafa Elshobary; Gamal Ammar; Ahmed Gaber; Walaa Alsanie; Abdallah Mansour; Rania El-Shenody. Impact of Commercial Seaweed Liquid Extract (TAM®) Biostimulant and Its Bioactive Molecules on Growth and Antioxidant Activities of Hot Pepper (Capsicum annuum). Plants 2021, 10, 1045 .
AMA StyleMohamed Ashour, Shimaa Hassan, Mostafa Elshobary, Gamal Ammar, Ahmed Gaber, Walaa Alsanie, Abdallah Mansour, Rania El-Shenody. Impact of Commercial Seaweed Liquid Extract (TAM®) Biostimulant and Its Bioactive Molecules on Growth and Antioxidant Activities of Hot Pepper (Capsicum annuum). Plants. 2021; 10 (6):1045.
Chicago/Turabian StyleMohamed Ashour; Shimaa Hassan; Mostafa Elshobary; Gamal Ammar; Ahmed Gaber; Walaa Alsanie; Abdallah Mansour; Rania El-Shenody. 2021. "Impact of Commercial Seaweed Liquid Extract (TAM®) Biostimulant and Its Bioactive Molecules on Growth and Antioxidant Activities of Hot Pepper (Capsicum annuum)." Plants 10, no. 6: 1045.
In marine ecosystems, fluctuations in surface-seawater carbon dioxide (CO2), significantly influence the whole metabolism of marine algae, especially during the early stages of macroalgal development. In this study, the response of the green alga Ulva fasciata for elevating ocean acidification was investigated using four levels of pCO2 ~ 280, 550, 750 and 1050 µatm. Maximum growth rate (6.6% day−1), protein (32.43 %DW) and pigment (2.9 mg/g) accumulation were observed at pCO2-550 with an increase of ~2-fold compared to control. On the other hand, lipid and carbohydrate contents recorded their maximum production (4.23 and 46.96 %DW, respectively) at pCO2-750 while control showed 3.70 and 42.37 %DW, respectively. SDS-PAGE showed the presence of unique bands in response to pCO2, especially at 550 µatm. Dominant associated bacteria was shifted from Halomonas hydrothermalis of control to Vibrio toranzoniae at pCO2-1050. These findings suggest that ocean acidification at 550 µatm might impose noticeable effects on growth, protein, pigments, and protein profile of U. fasciata, which could be a good source for fish farming. While, pCO2-750 was recommended for energetic purpose, due to its high lipid and carbohydrate contents.
Khouloud M. Barakat; Heba S. El-Sayed; Hanan M. Khairy; Mohamed A. El-Sheikh; Sarah A. Al-Rashed; Ibrahim A. Arif; Mostafa E. Elshobary. Effects of ocean acidification on the growth and biochemical composition of a green alga (Ulva fasciata) and its associated microbiota. Saudi Journal of Biological Sciences 2021, 28, 5106 -5114.
AMA StyleKhouloud M. Barakat, Heba S. El-Sayed, Hanan M. Khairy, Mohamed A. El-Sheikh, Sarah A. Al-Rashed, Ibrahim A. Arif, Mostafa E. Elshobary. Effects of ocean acidification on the growth and biochemical composition of a green alga (Ulva fasciata) and its associated microbiota. Saudi Journal of Biological Sciences. 2021; 28 (9):5106-5114.
Chicago/Turabian StyleKhouloud M. Barakat; Heba S. El-Sayed; Hanan M. Khairy; Mohamed A. El-Sheikh; Sarah A. Al-Rashed; Ibrahim A. Arif; Mostafa E. Elshobary. 2021. "Effects of ocean acidification on the growth and biochemical composition of a green alga (Ulva fasciata) and its associated microbiota." Saudi Journal of Biological Sciences 28, no. 9: 5106-5114.
This study aimed to understand the effect of commercial seaweed extract as a biofertilizer, named True-Algae-Max (TAM®), on the yield, nutritional, antioxidant, and cytotoxic activity of Eruca vesicaria. Three concentrations of TAM® (5, 10, and 15%) were studied by foliar spray over the two cultivation years (2016 and 2017) without any chemical fertilizer, along with a control consisting of synthetic nitrogen, phosphorus and potassium (NPK) fertilizers. The yield and composition of E. vesicaria were significantly improved in all treatments, particularly at 10% concentration of TAM®, which resulted in maximum yield (1.99 kg m−2) and significant amounts of chlorophyll, carotenoids, phenolic compounds, flavonoids and total nutrients. Compared to the NPK control, E. vesicaria grown with 10% of TAM® improved total antioxidant activity from 41.80 to 49.36 mg g−1 and cytotoxicity from 25.30 to 60.40% with an IC50 value 85.7 µg mL−1 against the hepatocellular carcinoma cell line (HepG2). These findings indicate that seaweed extract can generally be used as a safe potential multifunctional biofertilizer in the agricultural field. The use of seaweed as a biofertilizer could potentially help mitigate the adverse effects of main nutrient deficiencies, diminishing the use of chemical fertilizers.
Shimaa Hassan; Mohamed Ashour; Ahmed Soliman; Hesham Hassanien; Walaa Alsanie; Ahmed Gaber; Mostafa Elshobary. The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav. Sustainability 2021, 13, 4485 .
AMA StyleShimaa Hassan, Mohamed Ashour, Ahmed Soliman, Hesham Hassanien, Walaa Alsanie, Ahmed Gaber, Mostafa Elshobary. The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav. Sustainability. 2021; 13 (8):4485.
Chicago/Turabian StyleShimaa Hassan; Mohamed Ashour; Ahmed Soliman; Hesham Hassanien; Walaa Alsanie; Ahmed Gaber; Mostafa Elshobary. 2021. "The Potential of a New Commercial Seaweed Extract in Stimulating Morpho-Agronomic and Bioactive Properties of Eruca vesicaria (L.) Cav." Sustainability 13, no. 8: 4485.
Relative effects of spatio-seasonal variations on the quantitative and qualitative status of phytoplankton in 20 sampling locations of Temsah Lake were examined during winter and summer 2018. The study reveals that the population structure consisted of 105 species from 69 genera, with an annual average of 924155 cell/L. Bacillariophyceae formed the most dominant group with 42.45% of total phytoplankton with an average of 372379 cell/L. Navicula creptocephala was the most abundant species representing 19.16% of total diatoms and 7.78% of the whole standing crop with an annual average of 376879 cell/L. The highest phytoplankton abundance was in the Northern locations. Phytoplankton density showed a negative correlation of pH, temperature and TDS and positively correlated with nutrient content such as nitrate and silicate. In general, summer showed high indices value compared to winter and the diversity index in the current study ranged in moderate pollution range. The polluted state of the aquatic ecosystem in El-Temsah Lake during summer is comparatively higher than winter during the study period. Palmer’s and diversity indices showed that Northern sites were the most flourishing and polluted sites. The study also recorded a number of tolerate algae (13 genera and 8 species), which can be used as an indicator for different degrees of organic pollution. Genus and species Palmer’s Index scores of the northern site were in the range of ≥20 during summer, indicating most of the sampling locations showed probable to confirmed high level of organic pollution.
Mostafa Elshobary; Abdullah M. Attiah; Zenhom E. Salem; Dorya I. Essa. Phytoplankton population as alarming warning bioindicator of water pollution in El-Temsah Lake, Egypt. 2021, 1 .
AMA StyleMostafa Elshobary, Abdullah M. Attiah, Zenhom E. Salem, Dorya I. Essa. Phytoplankton population as alarming warning bioindicator of water pollution in El-Temsah Lake, Egypt. . 2021; ():1.
Chicago/Turabian StyleMostafa Elshobary; Abdullah M. Attiah; Zenhom E. Salem; Dorya I. Essa. 2021. "Phytoplankton population as alarming warning bioindicator of water pollution in El-Temsah Lake, Egypt." , no. : 1.
To achieve strong, successful and commercial aqua-biotechnological microalgae applications, screening, isolation, molecular identification, and physiological characterizations are needed. In the current study, a native cyanobacteria strain Arthrospira platensis NIOF17/003 was isolated from the surface water of El-Khadra Lake, a saline-alkaline lake located in Wadi El-Natrun, Egypt. The cyanobacterium was phylogenetically identified by 16S rRNA molecular marker and deposited in the GenBank database (accession number MW396472). The late exponential phase of A. platensis NIOF17/003 was reached at the 8th day of growth using Zarrouk medium, with a recorded dry weight (DW) of 0.845 g L−1. The isolated strain showed 52% of protein, 14% of carbohydrate, biomass productivity of 143.83 mg L−1 day−1, 8.5% of lipid, and lipid productivity of 14.37 mg L−1 day−1. In general, the values of cetane number, iodine value, cold filter plugging point (52.9, 85.5 g I2/100 g oil, and −2.2 °C, respectively) of the isolated fatty acid methyl esters are in accordance with those suggested by international standards. Besides, applying algal-free lipid (FL) as biodiesel byproduct in the production of rotifer (Brachionus plicatilis) revealed that a 0.6 g L−1 FL significantly increased the rotifer population females carrying eggs, confirming that FL can be used efficiently for B. plicatilis production. The current study concluded that the new isolate A. platensis NIOF17/003 is a promising strain for double sustainable use in biodiesel production and aquaculture feed.
Mohamed Zaki; Mohamed Ashour; Ahmed Heneash; Mohamed Mabrouk; Ahmed Alprol; Hanan Khairy; Abdelaziz Nour; Abdallah Mansour; Hesham Hassanien; Ahmed Gaber; Mostafa Elshobary. Potential Applications of Native Cyanobacterium Isolate (Arthrospira platensis NIOF17/003) for Biodiesel Production and Utilization of Its Byproduct in Marine Rotifer (Brachionus plicatilis) Production. Sustainability 2021, 13, 1769 .
AMA StyleMohamed Zaki, Mohamed Ashour, Ahmed Heneash, Mohamed Mabrouk, Ahmed Alprol, Hanan Khairy, Abdelaziz Nour, Abdallah Mansour, Hesham Hassanien, Ahmed Gaber, Mostafa Elshobary. Potential Applications of Native Cyanobacterium Isolate (Arthrospira platensis NIOF17/003) for Biodiesel Production and Utilization of Its Byproduct in Marine Rotifer (Brachionus plicatilis) Production. Sustainability. 2021; 13 (4):1769.
Chicago/Turabian StyleMohamed Zaki; Mohamed Ashour; Ahmed Heneash; Mohamed Mabrouk; Ahmed Alprol; Hanan Khairy; Abdelaziz Nour; Abdallah Mansour; Hesham Hassanien; Ahmed Gaber; Mostafa Elshobary. 2021. "Potential Applications of Native Cyanobacterium Isolate (Arthrospira platensis NIOF17/003) for Biodiesel Production and Utilization of Its Byproduct in Marine Rotifer (Brachionus plicatilis) Production." Sustainability 13, no. 4: 1769.
This study aimed to evaluate appropriate water sources to improve Solea aegyptiaca aquaculture from larval to the juvenile stage using two water sources (Eastern Harbour (EH) and MaxWell (MW)). Firstly, four microalgae's nutritional value (Nannochloropsis salina, N. oculata, Chlorella salina and Tetraselmis chuii) was assessed in both water sources. MW of high nitrate content enhanced the algal biomass and biochemical composition of all species compared to EH. MW‐N. salina showed the highest growth and biochemical contents among the investigated species and yielded Artemia franciscana with a considerable amount of arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) compared to EH. Secondly, Artemia enriched MW‐N salina was used to improve S. aegyptiaca quality in both water sources from 10–48 days posthatch at a density of 15 larvae L−1. The MW‐S. aegyptiaca exhibited a significant increase in morphometric parameters, albino percentages and recorded the highest ARA, EPA and DHA content (35.9, 6.1 and 15.9 µg g−1, respectively) compared to that reared on EH. The study reports evidence of albinism of MW‐S. aegyptiaca due to high dietary and ARA content. Overall, the seawater source has a significant impact on the whole food chain quality from microalgae to S. aegyptiaca.
Gihan M. El‐Khodary; Heba S. El‐Sayed; Hanan M. Khairy; Mohamed A. El‐Sheikh; Xianghui Qi; Mostafa E. Elshobary. Comparative study on growth, survival and pigmentation of Solea aegyptiaca larvae by using four different microalgal species with emphasize on water quality and nutritional value. Aquaculture Nutrition 2020, 27, 615 -629.
AMA StyleGihan M. El‐Khodary, Heba S. El‐Sayed, Hanan M. Khairy, Mohamed A. El‐Sheikh, Xianghui Qi, Mostafa E. Elshobary. Comparative study on growth, survival and pigmentation of Solea aegyptiaca larvae by using four different microalgal species with emphasize on water quality and nutritional value. Aquaculture Nutrition. 2020; 27 (2):615-629.
Chicago/Turabian StyleGihan M. El‐Khodary; Heba S. El‐Sayed; Hanan M. Khairy; Mohamed A. El‐Sheikh; Xianghui Qi; Mostafa E. Elshobary. 2020. "Comparative study on growth, survival and pigmentation of Solea aegyptiaca larvae by using four different microalgal species with emphasize on water quality and nutritional value." Aquaculture Nutrition 27, no. 2: 615-629.
In the present study, eight seaweeds (five red, two brown and one green) were collected and evaluated for dual biodiesel and/or bioethanol production. The highest significant lipid and carbohydrate contents were recorded in the brown seaweed Dilophus fasciola (4.92 and 37.97%dw, respectively). Three routes were investigated, namely R1 and R2 for direct production of biodiesel and bioethanol, respectively, from the whole biomass, in addition to R3 representing the sequential biodiesel and bioethanol production. D. fasciola showed a biodiesel yield of 35.04 mg g−1 dw, composed mainly of C16‐C18 fatty acids with a high saturation degree. All biodiesel characteristics complied with the recommended values of international standards. The maximum reducing sugar content (37.2 g L−1) and bioethanol productivity at 72 hours (0.165 g L−1 h−1) were recorded in the lipid‐free biomass (R3), which were 16.3% and 27.9%, respectively, higher than that of R2. Therefore, a maximum estimated total energy output of 9.96 MJ kg−1 was recorded in R3, which represented 6‐times and 28.3% higher than R1 and R2, respectively.
Mostafa E. Elshobary; Rania A. El‐Shenody; Abd El‐Fatah Abomohra. Sequential biofuel production from seaweeds enhances the energy recovery: A case study for biodiesel and bioethanol production. International Journal of Energy Research 2020, 45, 6457 -6467.
AMA StyleMostafa E. Elshobary, Rania A. El‐Shenody, Abd El‐Fatah Abomohra. Sequential biofuel production from seaweeds enhances the energy recovery: A case study for biodiesel and bioethanol production. International Journal of Energy Research. 2020; 45 (4):6457-6467.
Chicago/Turabian StyleMostafa E. Elshobary; Rania A. El‐Shenody; Abd El‐Fatah Abomohra. 2020. "Sequential biofuel production from seaweeds enhances the energy recovery: A case study for biodiesel and bioethanol production." International Journal of Energy Research 45, no. 4: 6457-6467.
The present study explores the effects of three bacteria, namely Rhizobium rosettiformans, Hydrogenophaga intermedia, Sphingopyxis terrae and their mix culture on the growth, pigment content, nutrient removal, lipid content and fatty acid composition of microalgae Tribonema sp. S. terrae and mix culture did not affect the biomass of Tribonema sp. noticeably while H. intermedia and R. rosettiformans significantly reduce the biomass. The removal rate of total phosphorous (TP) and total nitrogen (TN) were boosted to 98.34% and 94.64% by addition of S. terrae and Tribonema sp., respectively compared to control (90.13% and 86.20%, respectively). Moreover, S. terrae improved the chlorophyll b content significantly in Tribonema sp. to 1.17 mg/g by 18% higher than the control group. Addition of S. terrae and R. rosettiformans increased the lipid content of Tribonema sp. by 21% and 13%, respectively compared to the control. Regarding fatty acid (FA) profile and biodiesel properties, co-culture of S. terrae with Tribonema sp. showed high contents of unsaturated fatty acid (USFA) and C16-C18 FA, plus low saturated fatty acid (SFA) and polyunsaturated fatty acid (PUFA) contents that are preferable for excellent biodiesel performance. Hence, the S. terrae intervention has the most significant effect on nutrients removal, pigment content, lipid content and biodiesel quality.
Shuhao Huo; Sajid Basheer; Fen Liu; Mostafa Elshobary; Cunsheng Zhang; Jingya Qian; Ling Xu; Muhammad Arslan; Fengjie Cui; Xinyi Zan; Feifei Zhu; Bin Zou; Qingzhi Ding; Haile Ma. Bacterial intervention on the growth, nutrient removal and lipid production of filamentous oleaginous microalgae Tribonema sp. Algal Research 2020, 52, 102088 .
AMA StyleShuhao Huo, Sajid Basheer, Fen Liu, Mostafa Elshobary, Cunsheng Zhang, Jingya Qian, Ling Xu, Muhammad Arslan, Fengjie Cui, Xinyi Zan, Feifei Zhu, Bin Zou, Qingzhi Ding, Haile Ma. Bacterial intervention on the growth, nutrient removal and lipid production of filamentous oleaginous microalgae Tribonema sp. Algal Research. 2020; 52 ():102088.
Chicago/Turabian StyleShuhao Huo; Sajid Basheer; Fen Liu; Mostafa Elshobary; Cunsheng Zhang; Jingya Qian; Ling Xu; Muhammad Arslan; Fengjie Cui; Xinyi Zan; Feifei Zhu; Bin Zou; Qingzhi Ding; Haile Ma. 2020. "Bacterial intervention on the growth, nutrient removal and lipid production of filamentous oleaginous microalgae Tribonema sp." Algal Research 52, no. : 102088.
Power generation from the renewable biomass sources using microbial fuel cell (MFC) has attracted significant attention in recent years, while chemical energy stored in microalgae biomass has efficiently been used for the sustainable production of biofuels and other valuable bioproducts since the decades. The usage of these photosynthetic organisms in MFC can enhance the efficiency of MFC and provide a cost-effective and renewable approach for the bio-generation of electricity. Microalgae are commonly incorporated either with anode or cathode compartment of MFC to generate electron or oxygen, respectively. Despite microalgae-assisted MFC (MA-MFC) would be more sustainable than using MFC alone, further developments in such systems are still required for improving its efficiency and achieving a real-world application on a large scale. In this context, understanding in bio-electrochemical mechanism of MA-MFC, including electrons shuttle and oxygen generation, is very important. Moreover, many factors can limit the efficiency and performances of MA-MFCs that are needed to optimize in further research efforts. This review presents a comprehensive insight into MA-MFC, including the recent developments and potential challenges in this promising bio-electricity generating system. Specifically, it focuses a critical discussion on the configurations of MA-MFC, key operating parameters affecting performances of MA-MFC, challenges and prospective research works for improving the overall energy output of MA-MFC.
Mostafa E. Elshobary; Hossain M. Zabed; Junhua Yun; Guoyan Zhang; Xianghui Qi. Recent insights into microalgae-assisted microbial fuel cells for generating sustainable bioelectricity. International Journal of Hydrogen Energy 2020, 46, 3135 -3159.
AMA StyleMostafa E. Elshobary, Hossain M. Zabed, Junhua Yun, Guoyan Zhang, Xianghui Qi. Recent insights into microalgae-assisted microbial fuel cells for generating sustainable bioelectricity. International Journal of Hydrogen Energy. 2020; 46 (4):3135-3159.
Chicago/Turabian StyleMostafa E. Elshobary; Hossain M. Zabed; Junhua Yun; Guoyan Zhang; Xianghui Qi. 2020. "Recent insights into microalgae-assisted microbial fuel cells for generating sustainable bioelectricity." International Journal of Hydrogen Energy 46, no. 4: 3135-3159.
The present study evaluated the sequential biodiesel-bioethanol production from seaweeds. A total of 22 macroalgal species were collected at different seasons and screened based on lipid and carbohydrate contents as well as biomass production. The promising species was selected, based on the relative increase in energy compounds (REEC, %), for further energy conversion. Seasonal and annual biomass yields of the studied species showed significant variations. The rhodophyte Amphiroa compressa and the chlorophyte Ulva intestinalis showed the highest annual biomass yield of 75.2 and 61.5 g m−2 year−1, respectively. However, the highest annual carbohydrate productivity (ACP) and annual lipid productivity (ALP) were recorded for Ulva fasciata and Ulva intestinalis (17.0 and 3.0 g m−2 year−1, respectively). The later was selected for further studies because it showed 14.8% higher REEC value than Ulva fasciata. Saturated fatty acids (SAFs) showed 73.4%, with palmitic acid as a dominant fatty acid (43.8%). Therefore, biodiesel showed high saturation degree, with average degree of unsaturation (ADU) of 0.508. All the measured biodiesel characteristics complied the international standards. The first route of biodiesel production (R1) from Ulva intestinalis showed biodiesel recovery of 32.3 mg g−1 dw. The hydrolysate obtained after saccharification of the whole biomass (R2) and lipid-free biomass (R3) contained 1.22 and 1.15 g L−1, respectively, reducing sugars. However, bioethanol yield from R3 was 0.081 g g−1 dw, which represented 14.1% higher than that of R2. Therefore, application of sequential biofuel production using R3 resulted in gross energy output of 3.44 GJ ton−1 dw, which was 170.9% and 82.0% higher than R1 and R2, respectively. The present study recommended the naturally-grown Ulva intestinalis as a potential feedstock for enhanced energy recovery through sequential biodiesel-bioethanol production.
Mohamed E. H. Osman; Atef M. Abo-Shady; Mostafa E. ElShobary; Mahasen O. Abd El-Ghafar; Abd El-Fatah Abomohra. Screening of seaweeds for sustainable biofuel recovery through sequential biodiesel and bioethanol production. Environmental Science and Pollution Research 2020, 27, 32481 -32493.
AMA StyleMohamed E. H. Osman, Atef M. Abo-Shady, Mostafa E. ElShobary, Mahasen O. Abd El-Ghafar, Abd El-Fatah Abomohra. Screening of seaweeds for sustainable biofuel recovery through sequential biodiesel and bioethanol production. Environmental Science and Pollution Research. 2020; 27 (26):32481-32493.
Chicago/Turabian StyleMohamed E. H. Osman; Atef M. Abo-Shady; Mostafa E. ElShobary; Mahasen O. Abd El-Ghafar; Abd El-Fatah Abomohra. 2020. "Screening of seaweeds for sustainable biofuel recovery through sequential biodiesel and bioethanol production." Environmental Science and Pollution Research 27, no. 26: 32481-32493.
The phytoplankton community structure is affected by both ecological and spatial factors. Influences of these two factors on phytoplankton are still poorly understood. Here, the relative effects of spatio-temporal variations on the structuring and community of phytoplankton were examined in eight sites along Ismailia canal during wet winter and dry summer seasons, 2018. A total of 58 taxa were identified, belonging to five taxonomical groups, phytoplankton had an abundance ranging from 56,906 ind. L−1 in winter to 122,418 ind. L−1 in summer. Bacillariophyta was the leading and most dominant phyla representing about 45% of the total number of individuals owing to the great flourishing of Synedra ulna, Cyclotella kutzingiana, Cyclotella meneghiniana throughout the year. Spatially, sites 1, 2 and 7 showed the highest phytoplankton abundance, whereas site 6 showed the lowest community. Pearson correlation and Canonical correspondence analysis indicated that nitrate had a positive correlation with the phytoplankton community, however TDS showed a negative correlation with the algal community and silicate showed a positive correlation with Bacillariophyta. High relative Palmer’s Index scores were recorded in most of the sampling sites ranged from 13 to 21 particularly during summer, that coincides with Shannon–Weiner index results which indicating Ismailia canal suffers from organic pollution in most of the sampling sites.
Mostafa E. ElShobary; Dorya I. Essa; Abdullah Attiah; Zenhom E. Salem; Xianghui Qi. Algal community and pollution indicators for the assessment of water quality of Ismailia canal, Egypt. Stochastic Environmental Research and Risk Assessment 2020, 34, 1089 -1103.
AMA StyleMostafa E. ElShobary, Dorya I. Essa, Abdullah Attiah, Zenhom E. Salem, Xianghui Qi. Algal community and pollution indicators for the assessment of water quality of Ismailia canal, Egypt. Stochastic Environmental Research and Risk Assessment. 2020; 34 (7):1089-1103.
Chicago/Turabian StyleMostafa E. ElShobary; Dorya I. Essa; Abdullah Attiah; Zenhom E. Salem; Xianghui Qi. 2020. "Algal community and pollution indicators for the assessment of water quality of Ismailia canal, Egypt." Stochastic Environmental Research and Risk Assessment 34, no. 7: 1089-1103.
A coccoid green microalga, namely Chlorococcum minutum NIOF17/002, was isolated from the Delta region of Egypt, and further extracted with solvents both sequentially and individually to study antimicrobial and antioxidant activities of this microalga. The acetone extract gave maximum antimicrobial activity against the 5 bacterial pathogens and showed the lowest minimum inhibitory concentration against Salmonella typhi, while the methanol extract showed the highest reducing activity and total antioxidant capacity (3.92 and 9.83 mg ascorbic acid equivalents g−1, respectively) among the extracts. The total phenolic content (TPC) in methanol extract was found to be 6.65 mg gallic acid equivalents g−1, which was the highest TPC of the extracts. Acetone and methanol extracts were subsequently characterized using gas chromatography–mass spectrometry for identifying chemical compounds. The former was found to be enriched with palmitic and linolenic acids, and the latter contained mostly eugenol and methylated fatty acids. The presence of these bioactive compounds in C. minutum NIOF17/002 might make it beneficial as a source of antimicrobial and antioxidant agents.
Mostafa E. Elshobary; Rania A. El-Shenody; Mohamed Ashour; Hossain M. Zabed; Xianghui Qi. Antimicrobial and antioxidant characterization of bioactive components from Chlorococcum minutum. Food Bioscience 2020, 35, 100567 .
AMA StyleMostafa E. Elshobary, Rania A. El-Shenody, Mohamed Ashour, Hossain M. Zabed, Xianghui Qi. Antimicrobial and antioxidant characterization of bioactive components from Chlorococcum minutum. Food Bioscience. 2020; 35 ():100567.
Chicago/Turabian StyleMostafa E. Elshobary; Rania A. El-Shenody; Mohamed Ashour; Hossain M. Zabed; Xianghui Qi. 2020. "Antimicrobial and antioxidant characterization of bioactive components from Chlorococcum minutum." Food Bioscience 35, no. : 100567.
Generation of biodiesel from microalgae grown in wastewater can offer a cost-effective approach, whilst wastewaters usually do not contain the optimum concentrations of the essential nutrients and carbon sources that result in lowering the productivities of biomass and lipid. This study aimed to overcome this limitation by manipulating wastewater with various concentrations of nutrients (NO3−, PO43−, Cl− and SO42−) and three carbon sources either individually or in combination to cultivate Micractinium reisseri for biodiesel production. Initially, various dilutions of wastewater were tested and a concentration up to 75% of wastewater showed the highest biomass productivity (0.076 g L−1 d−1) and lipid productivity (0.014 g L−1 d−1). The optimum manipulating conditions for maximum lipid production and the highest productivity required 50% decrease in phosphorous from the concentration of the control medium and supplementation with 1.0 g L−1 of glucose. Under this condition, biomass and lipid productivities increased by 1.7 and 4-folds, respectively, compared to those observed in the control. Furthermore, phosphorous starvation condition in the presence of glucose significantly improved fatty acid profile in the biomass and biodiesel quality related parameters.
Mostafa E. Elshobary; Atef M. Abo-Shady; Hanan M. Khairy; Dorya Essa; Hossain M. Zabed; Xianghui Qi; Abd El-Fatah Abomohra. Influence of nutrient supplementation and starvation conditions on the biomass and lipid productivities of Micractinium reisseri grown in wastewater for biodiesel production. Journal of Environmental Management 2019, 250, 109529 .
AMA StyleMostafa E. Elshobary, Atef M. Abo-Shady, Hanan M. Khairy, Dorya Essa, Hossain M. Zabed, Xianghui Qi, Abd El-Fatah Abomohra. Influence of nutrient supplementation and starvation conditions on the biomass and lipid productivities of Micractinium reisseri grown in wastewater for biodiesel production. Journal of Environmental Management. 2019; 250 ():109529.
Chicago/Turabian StyleMostafa E. Elshobary; Atef M. Abo-Shady; Hanan M. Khairy; Dorya Essa; Hossain M. Zabed; Xianghui Qi; Abd El-Fatah Abomohra. 2019. "Influence of nutrient supplementation and starvation conditions on the biomass and lipid productivities of Micractinium reisseri grown in wastewater for biodiesel production." Journal of Environmental Management 250, no. : 109529.
Due to depletion of fossil fuel supplies and hazardous impacts on the global climate, clean renewable and sustainable energy production is being critically demanded. First-generation biofuels are mainly produced from edible crops and oilseeds. Because of competition with human food, first-generation biofuels are restricted in their ability to accomplish the global biofuel need, climate change amendment, and economic growth. Consequently, second-generation and third-generation biofuels were developed from nonedible feedstocks including lignocellulosic biomasses and microalgae, respectively, to overcome these challenges. However, algae are considered as a superior feedstock for biofuel production because of their diversity. Some of the major benefits of algae are their extremely fast growth rate and the ability of sequestration of carbon dioxide with high oil and carbohydrate contents that can be easily transformed into biodiesel or other gasoline components such as butanol. Biodiesel has been receiving globally growing consideration due to the liquid fuel needs and its potential as a biodegradable nontoxic substitute to petroleum diesel. In addition, butanol has become an attractive biofuel as a by-product of algal biomass processing after lipid extraction for biodiesel, due to its higher energy content, lower vapor pressure, and less hygroscopy than ethanol. This chapter reviews the current status of microalgae for biodiesel and butanol production as eco-friendly alternatives for liquid fossil fuels.
Abd El-Fatah Abomohra; Mostafa ElShobary. Biodiesel, Bioethanol, and Biobutanol Production from Microalgae. Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment 2019, 293 -321.
AMA StyleAbd El-Fatah Abomohra, Mostafa ElShobary. Biodiesel, Bioethanol, and Biobutanol Production from Microalgae. Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment. 2019; ():293-321.
Chicago/Turabian StyleAbd El-Fatah Abomohra; Mostafa ElShobary. 2019. "Biodiesel, Bioethanol, and Biobutanol Production from Microalgae." Microalgae Biotechnology for Development of Biofuel and Wastewater Treatment , no. : 293-321.
Ibrahim M. Abdelsalam; Mostafa ElShobary; Mohamed M. ElAdawy; Mohammed Nagah. Utilization of Multi-Tasking Non-Edible Plants for Phytoremediation and Bioenergy Source-A Review. Phyton 2019, 88, 69 -90.
AMA StyleIbrahim M. Abdelsalam, Mostafa ElShobary, Mohamed M. ElAdawy, Mohammed Nagah. Utilization of Multi-Tasking Non-Edible Plants for Phytoremediation and Bioenergy Source-A Review. Phyton. 2019; 88 (2):69-90.
Chicago/Turabian StyleIbrahim M. Abdelsalam; Mostafa ElShobary; Mohamed M. ElAdawy; Mohammed Nagah. 2019. "Utilization of Multi-Tasking Non-Edible Plants for Phytoremediation and Bioenergy Source-A Review." Phyton 88, no. 2: 69-90.
Screening of native microalgal species for different biotechnological applications is a key step to achieve a strong algal database with successful commercial applications. In the present study, a new strain namely Nannochloropsis oceanica NIOF15/001 was isolated and identified phylogenetically using rbcL and the newly designed Cyt-c as an alternative molecular marker. The late exponential phase of the isolate was recorded at the 10th day of growth in f/2 medium, with a maximum cellular dry weight (CDW) of 0.722 g L−1 and relatively high lipid content of 37.7% of CDW. The isolate showed biomass and lipid productivities of 75.6 and 28.6 mg L−1 day−1, respectively. The recorded biomass productivity in the current study was 1.4, 3.0, 3.6 and 2.0 times higher than that recorded in the previous studies for N. oceanica IMET, N. oceanica CASACC201, N. oceanica DUT01 and N. oceanica CCNM 1081, respectively. In general, values of cetane number (52.3), iodine value (94.7 g I2/100 g oil), cold filter plugging point (4.7 °C) and kinematic viscosity (3.97 mm2 s−1) of the produced fatty acid methyl esters complied with those recommended by international standards. Furthermore, application of 0.1 g L−1 of lipid-free dry biomass enhanced Artemia survival by 500% and growth by 40%. The present study suggested the new isolate N. oceanica NIOF15/001 as a promising marine microalga for dual use in aquaculture and biodiesel production.
Mohamed Ashour; Mostafa E. Elshobary; Rania El-Shenody; Abd el Wahahab Kamil; Abd El-Fatah Abomohra. Evaluation of a native oleaginous marine microalga Nannochloropsis oceanica for dual use in biodiesel production and aquaculture feed. Biomass and Bioenergy 2018, 120, 439 -447.
AMA StyleMohamed Ashour, Mostafa E. Elshobary, Rania El-Shenody, Abd el Wahahab Kamil, Abd El-Fatah Abomohra. Evaluation of a native oleaginous marine microalga Nannochloropsis oceanica for dual use in biodiesel production and aquaculture feed. Biomass and Bioenergy. 2018; 120 ():439-447.
Chicago/Turabian StyleMohamed Ashour; Mostafa E. Elshobary; Rania El-Shenody; Abd el Wahahab Kamil; Abd El-Fatah Abomohra. 2018. "Evaluation of a native oleaginous marine microalga Nannochloropsis oceanica for dual use in biodiesel production and aquaculture feed." Biomass and Bioenergy 120, no. : 439-447.
The biosynthesis of two polyketides, atranorin and fumarprotocetraric acid, produced from a lichen-forming fungus, Cladonia rangiferina (L.) F. H. Wigg. was correlated with the expression of eight fungal genes (CrPKS1, CrPKS3, CrPKS16, Catalase (CAT), Sugar Transporter (MFsug), Dioxygenase (YQE1), C2H2 Transcription factor (C2H2), Transcription Factor PacC (PacC), which are thought to be involved in polyketide biosynthesis, and one algal gene, NAD-dependent deacetylase sirtuin 2 (AsNAD)), using laser microdissection (LMD). The differential gene expression levels within the thallus tissue layers demonstrate that the most active region for potential polyketide biosynthesis within the lichen is the outer apical region proximal to the photobiont but some expression also occurs in reproductive tissue. This is the first study using laser microdissection to explore gene expression of these nine genes and their location of expression; it provides a proof-of-concept for future experiments exploring tissue-specific gene expression within lichens; and it highlights the utility of LMD for use in lichen systems.
Mostafa E. Elshobary; Michael G. Becker; Jenna L. Kalichuk; Ainsley C. Chan; Mark F. Belmonte; Michele D. Piercey-Normore. Tissue-specific localization of polyketide synthase and other associated genes in the lichen, Cladonia rangiferina, using laser microdissection. Phytochemistry 2018, 156, 142 -150.
AMA StyleMostafa E. Elshobary, Michael G. Becker, Jenna L. Kalichuk, Ainsley C. Chan, Mark F. Belmonte, Michele D. Piercey-Normore. Tissue-specific localization of polyketide synthase and other associated genes in the lichen, Cladonia rangiferina, using laser microdissection. Phytochemistry. 2018; 156 ():142-150.
Chicago/Turabian StyleMostafa E. Elshobary; Michael G. Becker; Jenna L. Kalichuk; Ainsley C. Chan; Mark F. Belmonte; Michele D. Piercey-Normore. 2018. "Tissue-specific localization of polyketide synthase and other associated genes in the lichen, Cladonia rangiferina, using laser microdissection." Phytochemistry 156, no. : 142-150.
Microalgae cultivation offers an effective solution for wastewater treatment, as they provide a tertiary bio-treatment coupled with the production of valuable biomass, which can be further used for different purposes. Using wastewater for microalgal cultivation is beneficial for minimizing the use of water, reducing the cost of nutrient addition and removing nitrogen and phosphorus from wastewater. Lipids obtained from marine Nannochloropsis oculata and Tetraselmis chuii microalgae have received growing attention for the production of biofuels. In the present study, the effluent of El-Malyaa Company (EMC) and Salt and Soda Company (ESC) were used as growth media for the two tested oleaginous marine microalgae. Wastewater was used in different ratios with sterilized seawater, or mixed with synthetic medium (F/2). The growth was evaluated by optical density and dry weight, total lipids content and productivity were also determined. The results showed that the tested species were capable of growing on the effluent wastewater of the two companies. In addition, dilution of the industrial wastewater with seawater or its mixing with synthetic culture medium (F/2) increased growth of the two tested marine microalgae. The maximum growth was recorded using a ratio of 25:75 of F/2 medium: the effluent of both companies. However, the highest lipid content and lipid productivity were recorded using a ratio of 75:25 of F/2 medium: the effluent of both companies. Therefore, this study suggests that it is possible to utilize a mixture of industrial wastewater and synthetic medium for potential biomass and lipid production from microalgae for biofuel production.
Mostafa ElShobary. Potential cultivation of halophilic oleaginous microalgae on industrial wastewater. Egyptian Journal of Botany 2018, 1 .
AMA StyleMostafa ElShobary. Potential cultivation of halophilic oleaginous microalgae on industrial wastewater. Egyptian Journal of Botany. 2018; ():1.
Chicago/Turabian StyleMostafa ElShobary. 2018. "Potential cultivation of halophilic oleaginous microalgae on industrial wastewater." Egyptian Journal of Botany , no. : 1.
Atef AboShady; Hanan Khairy; Abd Abomohra; Mostafa Elshobary; Dorya Essa. Influence of algal biotreated wastewater on some growth parameters and metabolites of Vicia faba. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 2017, 1 .
AMA StyleAtef AboShady, Hanan Khairy, Abd Abomohra, Mostafa Elshobary, Dorya Essa. Influence of algal biotreated wastewater on some growth parameters and metabolites of Vicia faba. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany). 2017; ():1.
Chicago/Turabian StyleAtef AboShady; Hanan Khairy; Abd Abomohra; Mostafa Elshobary; Dorya Essa. 2017. "Influence of algal biotreated wastewater on some growth parameters and metabolites of Vicia faba." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) , no. : 1.