Dr. Anagnostopoulos has been a researcher in the field of X-ray emission spectroscopy for over thirty-five years and has focused on various topics. These include particle-induced X-ray emission, the study of exotic atoms and molecules through X-ray emission cascades, fundamental X-ray parameters, high-resolution X-ray emission spectroscopy in the laboratory, X-ray fluorescence imaging (MA-XRF), the utilization of artificial intelligence for MA-XRF data analysis, and the implementation of these techniques in cultural heritage applications.