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Trihelix transcription factors play important roles in plant growth, development and various stress responses. In this study, we identified 32 trihelix family genes (DoGT) in the important Chinese medicinal plant Dendrobium officinale. These trihelix genes could be classified into five different subgroups. The gene structure and conserved functional domain of these trihelix genes were similar in the same subfamily but diverged between different subfamilies. Various stresses responsive cis-elements presented in the promoters of DoGT genes, suggesting that the trihelix genes might respond to the environmental stresses. Expressional changes of DoGT genes in three tissues and under cold treatment suggested that trihelix genes were involved in diverse functions during D. officinale development and cold tolerance. This study provides novel insights into the phylogenetic relationships and functions of the D. officinale trihelix genes, which will aid future functional studies investigating the divergent roles of trihelix genes belonging to other species.
Yan Tong; Hui Huang; Yuhua Wang. Genome-Wide Analysis of the Trihelix Gene Family and Their Response to Cold Stress in Dendrobium officinale. Sustainability 2021, 13, 2826 .
AMA StyleYan Tong, Hui Huang, Yuhua Wang. Genome-Wide Analysis of the Trihelix Gene Family and Their Response to Cold Stress in Dendrobium officinale. Sustainability. 2021; 13 (5):2826.
Chicago/Turabian StyleYan Tong; Hui Huang; Yuhua Wang. 2021. "Genome-Wide Analysis of the Trihelix Gene Family and Their Response to Cold Stress in Dendrobium officinale." Sustainability 13, no. 5: 2826.
Dendrobium catenatum is a member of epiphytic orchids with extensive range of pharmacological properties and ornamental values. Superoxide dismutase (SOD), a key member of antioxidant system, plays a vital role in protecting plants against oxidative damage caused by various biotic and abiotic stresses. So far, little is known about the SOD gene family in D. catenatum. In this study, eight SOD genes, including four Cu/ZnSODs, three FeSODs and one MnSOD, were identified in D. catenatum genome. Phylogenetic analyses of SOD proteins in D. catenatum and several other species revealed that these SOD proteins can be assigned to three subfamilies based on their metal co-factors. Moreover, the similarities in conserved motifs and gene structures in the same subfamily corroborated their classification and inferred evolutionary relationships. There were many hormone and stress response elements in DcaSODs, of which light responsiveness elements was the largest group. All DcaSODs displayed tissue-specific expression patterns and exhibited abundant expression levels in flower and leaf. According to public RNA-seq data and qRT-PCR analysis showed that the almost DcaSODs, except for DcaFSD2, were highly expressed under cold and drought treatments. Under heat, light, and salt stresses, DcaCSD1, DcaCSD2, DcaCSD3 were always significantly up-regulated, which may play a vital role in coping with various stresses. The expression levels of DcaFSD1 and DcaFSD2 were promoted by high light, suggesting their important roles in light response. These findings provided valuable information for further research on DcaSODs in D. catenatum.
Hui Huang; Hui Wang; Yan Tong; Yuhua Wang. Insights into the Superoxide Dismutase Gene Family and Its Roles in Dendrobium catenatum under Abiotic Stresses. Plants 2020, 9, 1452 .
AMA StyleHui Huang, Hui Wang, Yan Tong, Yuhua Wang. Insights into the Superoxide Dismutase Gene Family and Its Roles in Dendrobium catenatum under Abiotic Stresses. Plants. 2020; 9 (11):1452.
Chicago/Turabian StyleHui Huang; Hui Wang; Yan Tong; Yuhua Wang. 2020. "Insights into the Superoxide Dismutase Gene Family and Its Roles in Dendrobium catenatum under Abiotic Stresses." Plants 9, no. 11: 1452.
Premise Camellia reticulata , which is native to southwestern China, is an economically important plant belonging to the family Theaceae. We developed expressed sequence tag–simple sequence repeat (EST ‐SSR ) markers for C. reticulata , which can be used to investigate its genetic diversity, population structure, and evolutionary history. Methods and Results We detected 4780 SSR s in C. reticulata from Camellia RNA ‐Seq data deposited in the National Center for Biotechnology Information's expressed sequence tags database (dbEST ). Primer pairs for 70 SSR loci were designed and used for PCR amplification using 90 individuals from four populations of C. reticulata . Of these loci, 50 microsatellite markers were successfully identified, including 11 polymorphic markers. The allele number per locus ranged from two to seven (mean = 4.182), and the levels of observed and expected heterozygosity ranged from 0.044 to 0.567 and from 0.166 to 0.642, respectively. Eleven primer pairs amplified PCR products in three other species of Camellia (C. saluenensis , C. pitardii , and C. yunnanensis ). Conclusions The set of microsatellite markers developed here can be used to study the genetic variation and population structure of C. reticulata and related species and thereby help to develop conservation strategies for this species.
Yan Tong; Li-Zhi Gao. Development and characterization of EST ‐ SSR markers for Camellia reticulata. Applications in Plant Sciences 2020, 8, 1 .
AMA StyleYan Tong, Li-Zhi Gao. Development and characterization of EST ‐ SSR markers for Camellia reticulata. Applications in Plant Sciences. 2020; 8 (5):1.
Chicago/Turabian StyleYan Tong; Li-Zhi Gao. 2020. "Development and characterization of EST ‐ SSR markers for Camellia reticulata." Applications in Plant Sciences 8, no. 5: 1.