Increased salinity caused by saltwater intrusion or runoff from de-icing salts can severely affect freshwater vegetation and deteriorate aquatic ecosystems. These habitats can be restored with freshwater ecotypes (locally adapted populations) that tolerate above-normal salinity. Vallisneria americana is a prominent species in many freshwater ecosystems that responds differently to abiotic conditions such as substrate composition and fertility, so, in this study, we evaluated the effects of salt stress on 24 ecotypes of V. americana. Instant Ocean aquarium salt was used to create saline solutions (0.2 to 20.0 parts per thousand (ppt)), then plants were abruptly exposed to these solutions and maintained in these concentrations for five weeks before being visually assessed for quality and destructively harvested. Analysis of variance and nonlinear regression were used to calculate LC₅₀ values—the lethal concentration of salt that reduced plant biomass and quality by 50% compared to control treatment. Growth rate and visual quality varied significantly among ecotypes, and ecotypes that were most and least sensitive to salt had 50% biomass reductions at 0.47 and 9.10 ppt, respectively. All ecotypes survived 10.0 ppt salinity concentration but none survived at 20.0 ppt, which suggests that the maximum salinity concentration tolerated by these ecotypes is between 15.0 and 20.0 ppt.

This study investigates the use of unmanned aerial systems (UAS) mapping for monitoring the efficacy of invasive aquatic vegetation (AV) management on a floating-leaved AV species, Nymphoides cristata (CFH). The study site consists of 48 treatment plots (TPs). Based on six unique flights over two days at three different flight altitudes while using both a multispectral and RGB sensor, accuracy assessment of the final object-based image analysis (OBIA)-derived classified images yielded overall accuracies ranging from 89.6% to 95.4%. The multispectral sensor was significantly more accurate than the RGB sensor at measuring CFH areal coverage within each TP only with the highest multispectral, spatial resolution (2.7 cm/pix at 40 m altitude). When measuring response in the AV community area between the day of treatment and two weeks after treatment, there was no significant difference between the temporal area change from the reference datasets and the area changes derived from either the RGB or multispectral sensor. Thus, water resource managers need to weigh small gains in accuracy from using multispectral sensors against other operational considerations such as the additional processing time due to increased file sizes, higher financial costs for equipment procurements, and longer flight durations in the field when operating multispectral sensors.