Large amounts of chemical fertilizers are still used to suppress pathogens and boost agricultural productivity and food generation. However, their use can cause harmful environmental imbalance. Furthermore, plants typically absorb limited amounts of the nutrients provided by chemical fertilizers. Recent studies are recommending the use of microbiota present in the soil in different formulations, considering that several microorganisms are found in nature in association with plants in a symbiotic, antagonistic, or synergistic way. This ecological alternative is positive because no undesirable significant alterations occur in the environment while stimulating plant nutrition development and protection against damage caused by control pathogens. Therefore, this review presents a comprehensive discussion regarding endophytic and rhizospheric microorganisms and their interaction with plants, including signaling and bio-control processes concerning the plant’s defense against pathogenic spread. A discussion is provided about the importance of these bioinputs as a microbial resource that promotes plant development and their sustainable protection methods aiming to increase resilience in the agricultural system. In modern agriculture, the manipulation of bioinputs through Rhizobium contributes to reducing the effects of greenhouse gases by managing nitrogen runoff and decreasing nitrous oxide. Additionally, mycorrhizal fungi extend their root systems, providing plants with greater access to water and nutrients.

The extraction of bioactive compounds, such as flavonoids and coumarins, from natural sources has gained significant attention due to their potential health benefits. This review aims to explore the application of high-pressure extraction processes, particularly supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE), for obtaining flavonoids and coumarins from flower seeds. These techniques offer a greener, more efficient alternative to conventional extraction methods, minimizing the use of harmful solvents and improving the yield and purity of the target compounds. Flower seeds, a rich source of bioactive molecules, are an underutilized reservoir for these valuable compounds. For example, seeds from plants such as Calendula officinalis (calendula) and Helianthus annuus (sunflower) are rich in flavonoids and coumarins. The proposed review will examine the influence of extraction parameters—such as temperature, pressure, solvent choice, and extraction time—on the yield and quality of flavonoids and coumarins. This review aims to provide a comprehensive understanding of high-pressure extraction methods and optimize protocols for the efficient, sustainable extraction of flavonoids and coumarins from flower seeds.