Hypertension is highly prevalent among the Lebanese adult population and is indeed the major cause of mortality in Lebanon. Traditional use of antihypertensive medicinal plants has long been practiced. The aim of this study is to document this traditional knowledge and clinically test the antihypertensive capacity of three of the most commonly used wild plant species Mentha longifolia, Viola odorata and Urtica dioica. Ethno-pharmacological data was collected by personal interviews with herbalists and traditional healers using a semi structured survey questionnaire and assessing relative frequency of citation (RFC). The clinical study was conducted by a randomized, blind, placebo-controlled trial in 29 subjects with mild hypertension distributed in four groups, three plant extract treatments and one placebo. Systolic (SBP) and diastolic blood pressures (DBP) as well as mean arterial blood pressures (MAP) were monitored at weeks 4, 8, 12 and 16 during the treatment with 300 mL/day of plant extract. Results showed that M. longifolia, U. dioica and V. odorata exhibited the highest values of RCF (0.95) followed by Allium ampeloprasum (0.94), Apium graveolens (0.92) and Crataegus azarolus (0.90). The clinical trial revealed dose- and duration-dependent significant reductions in SBP, DBP and MAP of subjects treated with M. longifolia, U. dioica or V. odorata. Our findings indicate that extracts of these plants present an effective, safe and promising potential as a phyto-therapuetical approach for the treatment of mild hypertension. More research on the phytochemistry, pharmacological effects and the underlying mechanisms is necessary.

Nowadays, the increasing demand for concrete is causing serious environmental impact including pollution and waste generation, rapid depletion of natural resources, and increased CO₂ emission. Incorporating natural fibers in concrete can contribute toward environmental sustainability. This paper is concerned with the use of natural fibers obtained from the plant species Phragmites australis (PA). The plant is invasive, and rapidly grows abundantly along rivers and waterways, causing major ecological problems. This research is part of a wide range investigation on the use of natural fibers produced from the stem of PA plants in concrete. Using a machine, plant stems were crushed into fibers measuring 40 mm in length and 2 mm in width, and treated with 4% NaOH solution for 24 h. A total of four concrete mixes were prepared with varying additions of treated fibers, ranging from 0% to 1.5% (by volume) with water to cement ratio of 0.5% (by volume). Concrete specimens were tested at 3, 7, and 28 days. Testing included compressive strength, density, total water absorption, and capillary water absorption. The results show that incorporating PA natural fibers reduces the water absorption by total immersion and capillary action by up to 45%. Moreover, there is a negligible decrease in concrete density and strength when fibers were added. It is concluded that adding up to 1.5% natural PA fibers to concrete is a feasible strategy to produce an eco-friendly material which can be used in the production of sustainable building material with adequate mechanical and durability performance.

Introduction: In Lebanon, wild leafy vegetables (WLVs) continue to be an essential component of people's diet. Nevertheless, little ethnobotanical research has addressed this important potential source for food and nutrition.Methods: In this study, an ethnobotanical survey was carried out between 2018 and 2022 across 21 villages/towns involving 151 local informants by semi-structured interviews.Results and discussion: A list of 158 plant species which was still gathered and consumed by traditional Lebanese communities was compiled. The species belonged to 21 families and the most diverse families were Asteraceae (77 species), Brassicaceae (22), and Apiaceae (11). Native species accounted for 147 of the cited species, five of which were endemics to Lebanon and Syria. According to the Relative Frequency of Citation (RFC) index, Anchusa azurea, Centaurea hyalolepis, Chondrilla juncea, Eruca vesicaria, Pseudopodospermum molle, Pseudopodospermum papposum, and Taraxacum sect. Taraxacum were reported for the first time among the top popular species. Basal rosettes were the main parts consumed both raw and/or cooked. Informants perceived a decline in availability primarily due to habitat loss and land degradation. According to the International Union for Conservation of Nature (IUCN), six of cited species were classified as globally threatened (EN or VU), five of which were narrow endemics. These species scored substantial RFC values. Findings indicate that WEVs of Lebanon offer a promising potential to support food diversity and dietary quality of traditional communities and that threatened endemic species present a high conservation priority. Future research on nutritional contents of these species and highly rated WLVs is recommended.

Inula viscosa is a widely used plant in traditional Mediterranean and Middle Eastern medicine for various illnesses. I. viscosa has been shown to have anticancer effects against various cancers, but its effects against lung cancer have been under limited investigation. At the same time, I. viscosa is rich in terpenoids whose anti-lung cancer effects have been poorly investigated. This study aimed to examine the potential anticancer properties of methanolic and aqueous extracts of stems and leaves of I. viscosa and its terpenoid-rich fraction against human lung cancer A549 cells. Results showed that the methanolic extracts of I. viscosa had significantly higher polyphenol and flavonoid content and radical scavenging capacity than the aqueous extracts. In addition, leaves methanolic extracts (IVLM) caused the highest reduction in viability of A549 cells among all the extracts. IVLM also reduced the viability of human ovarian SK-OV-3, breast MCF-7, liver HepG2, and colorectal HCT116 cancer cells. A terpenoid-rich I. viscosa fraction (IVL DCM), prepared by liquid-liquid separation of IVLM in dichloromethane (DCM), displayed a substantial reduction in the viability of A549 cells (IC₅₀ = 27.8 ± 1.5 µg/mL at 48 h) and the panel of tested cancerous cell lines but was not cytotoxic to normal human embryonic fibroblasts (HDFn). The assessment of IVL DCM phytochemical constituents using GC-MS analysis revealed 21 metabolites, highlighting an enrichment in terpenoids, such as lupeol and its derivatives, caryophyllene oxide, betulin, and isopulegol, known to exhibit proapoptotic and antimetastatic functions. IVL DCM also showed robust antioxidant capacity and decent polyphenol and flavonoid contents. Furthermore, Western blotting analysis indicated that IVL DCM reduced proliferation (reduction of proliferation marker Ki67 and induction of proliferation inhibitor proteins P21 and P27), contaminant with P38 MAP kinase activation, and induced the intrinsic apoptotic pathway (P53/BCL2/BAX/Caspase3/PARP) in A549 cells. IVL DCM also reduced the migration of A549 cells, potentially by reducing FAK activation. Future identification of anticancer metabolites of IVL DCM, especially terpenoids, is recommended. These data place I. viscosa as a new resource of herbal anticancer agents.

During surveys in 2020 on the Lebanese western slopes of Mount Hermon, we detected Ferula biverticellata J.Thiébaut, which is new to the flora of Lebanon. The species has a distribution extending from Mount Hermon, the Golan, and the Hauran in South Syria to the Negev and Transjordan. We discuss the range of this southern Levantine element, its presence in Lebanon, and its conservation status.