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Dr. Michail Michailidis
Laboratory of Pomology, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloníki, Greece

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0 Plant Nutrition
0 plant proteomics
0 plant metabolomics
0 fruit quality
0 fruit science

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Journal article
Published: 09 June 2021 in Food Chemistry
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Maturity is one of the most important factors associated with the quality of olive products, however the molecular events underlying olive drupe development remain poorly characterized. Using proteomic and metabolomic approaches, this study investigated the changes in the olive drupes (cv. Chondrolia Chalkidikis) across six developmental stages (S1-S6) that characterize the dynamics of fruit growth and color. Primary metabolites, including carbohydrates and organic acids (i.e., xylose, malic acid), showed significant accumulation in the black maturation stage. Temporal changes in various secondary metabolites (e.g., oleuropein, oleacin and tyrosol) were also observed. Proteins involved in oxidation–reduction (i.e., LOX1/5), carbohydrate metabolism (i.e., GLUA, PG) and photosynthesis (i.e., chlorophyll a-b binding proteins) significantly altered in the turning black compared to the green mature stage. By providing the first proteometabolomic study of olive drupe development, this investigation offers a novel framework for further studies on this economically relevant crop.

ACS Style

Evangelos Karagiannis; Michail Michailidis; Christina Skodra; George Stamatakis; Marilena Dasenaki; Ioannis Ganopoulos; Martina Samiotaki; Nikolaos S. Thomaidis; Athanassios Molassiotis; Georgia Tanou. Proteo-metabolomic journey across olive drupe development and maturation. Food Chemistry 2021, 363, 130339 .

AMA Style

Evangelos Karagiannis, Michail Michailidis, Christina Skodra, George Stamatakis, Marilena Dasenaki, Ioannis Ganopoulos, Martina Samiotaki, Nikolaos S. Thomaidis, Athanassios Molassiotis, Georgia Tanou. Proteo-metabolomic journey across olive drupe development and maturation. Food Chemistry. 2021; 363 ():130339.

Chicago/Turabian Style

Evangelos Karagiannis; Michail Michailidis; Christina Skodra; George Stamatakis; Marilena Dasenaki; Ioannis Ganopoulos; Martina Samiotaki; Nikolaos S. Thomaidis; Athanassios Molassiotis; Georgia Tanou. 2021. "Proteo-metabolomic journey across olive drupe development and maturation." Food Chemistry 363, no. : 130339.

Brief research report article
Published: 09 June 2021 in Frontiers in Nutrition
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The aim of the present study was to investigate the impact of exogenous melatonin (0. 5 mM) application through pre-harvest foliar spray and postharvest immersion, alone or in combination, on ripening parameters of sweet cherry (cv. Ferrovia) fruit and their relationship with bioactive compounds and gene expression at harvest as well after cold storage (0°C) for 12 days and subsequent room temperature (20°C) exposure for 8 h. Although several ripening traits were not influenced by melatonin, the combining pre- and post-harvest treatments delayed fruit softening at post-cold period. Preharvest spray with melatonin depressed fruit respiration at time of harvest while all applied treatments induced respiratory activity following cold, indicating that this anti-ripening action of melatonin is reversed by cold. Several genes related to the tricarboxylic acid cycle, such as PaFUM, PaOGDH, PaIDH, and PaPDHA1 were upregulated in fruit exposed to melatonin, particularly following combined pre- and post-harvest application. The accumulation of phenolic compounds, such as neochlorogenic acid, chlorogenic acid, epicatechin, procyanidin B1, procyanidin B2+B4, cyanidin-3-O-galactoside, and cyanidin-3-O-rutinoside along with the expression of several genes involved in phenols biosynthesis, such as PaSK, PaPAL, Pa4CL, PaC4H, and PaFNR were at higher levels in melatonin-treated cherries at harvest and after cold exposure, the highest effects being observed in fruits subjected to both pre- and post-harvest treatments. This study provides a comprehensive understanding of melatonin-responsive ripening framework at different melatonin application conditions and sweet cherry stages, thereby helps to understand the action of this molecule in fruit physiology.

ACS Style

Michail Michailidis; Georgia Tanou; Eirini Sarrou; Evangelos Karagiannis; Ioannis Ganopoulos; Stefan Martens; Athanassios Molassiotis. Pre- and Post-harvest Melatonin Application Boosted Phenolic Compounds Accumulation and Altered Respiratory Characters in Sweet Cherry Fruit. Frontiers in Nutrition 2021, 8, 1 .

AMA Style

Michail Michailidis, Georgia Tanou, Eirini Sarrou, Evangelos Karagiannis, Ioannis Ganopoulos, Stefan Martens, Athanassios Molassiotis. Pre- and Post-harvest Melatonin Application Boosted Phenolic Compounds Accumulation and Altered Respiratory Characters in Sweet Cherry Fruit. Frontiers in Nutrition. 2021; 8 ():1.

Chicago/Turabian Style

Michail Michailidis; Georgia Tanou; Eirini Sarrou; Evangelos Karagiannis; Ioannis Ganopoulos; Stefan Martens; Athanassios Molassiotis. 2021. "Pre- and Post-harvest Melatonin Application Boosted Phenolic Compounds Accumulation and Altered Respiratory Characters in Sweet Cherry Fruit." Frontiers in Nutrition 8, no. : 1.

Journal article
Published: 08 June 2021 in Plant Physiology and Biochemistry
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The benefits of silicon against abiotic stress in different annual plant species have been described in many studies, however the regulation of ripening of fruit tree crops by silicon remains largely uncharacterized. Therefore, the present study aimed to explore the impact of foliar silicon application in the apple (cv. ‘Fuji’) fruit ripening traits along with the effect of silicon in the nutrient and metabolic changes in the fully expanded leaves, annual shoots, fruit outer pericarp (peel) and fruit mesocarp (skin) tissues. Data indicated that fruit firmness and apple peel color attributes, such as redness (a*) and percentage of red-blushed surface were induced by silicon application. Moreover, several fruit ripening traits, such as titratable acidity, soluble solid content and respiration rate were unaffected by silicon. Endogenous silicon level in leaves shoots and peel tissues were increased by exogenously applied silicon while several elements (i.e., P, Mg, Mn, Fe and Cu) were altered in the tested tissues that exposed to silicon. In addition, silicon increased the accumulation of total phenolic and total anthocyanin compounds in the various apple tissues. The level of various primary metabolites including sorbitol, fructose, maltose cellobiose, malic acid, phosphoric acid and gluconic acid was also notably affected by silicon in a tissue-specific manner. Overall, this study provides a valuable resource for future research, aiming in the elucidation of the role of silicon in fruit tree physiology.

ACS Style

Evangelos Karagiannis; Michail Michailidis; Christina Skodra; Athanassios Molassiotis; Georgia Tanou. Silicon influenced ripening metabolism and improved fruit quality traits in apples. Plant Physiology and Biochemistry 2021, 166, 270 -277.

AMA Style

Evangelos Karagiannis, Michail Michailidis, Christina Skodra, Athanassios Molassiotis, Georgia Tanou. Silicon influenced ripening metabolism and improved fruit quality traits in apples. Plant Physiology and Biochemistry. 2021; 166 ():270-277.

Chicago/Turabian Style

Evangelos Karagiannis; Michail Michailidis; Christina Skodra; Athanassios Molassiotis; Georgia Tanou. 2021. "Silicon influenced ripening metabolism and improved fruit quality traits in apples." Plant Physiology and Biochemistry 166, no. : 270-277.

Journal article
Published: 06 June 2021 in Horticulturae
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The possible role of an early calcium application via sprays (0.25, 0.5 and 1M CaCl2) on dormant buds to improve sweet cherry (cv. Ferrovia) fruit quality at harvest was investigated. Fruit quality characteristics were also investigated in response to the age of spurs, the ripening stage, and their interactions. Results indicate that calcium enters the dormant flower buds and the phloem but not to the dormant vegetative buds. At harvest, the levels of Zn, Mn, and Cu were declined in fruits by increasing CaCl2 doses of sprays. Fruit respiratory activity was higher and on–tree fruit cracking was lower in red-colour (unripe) cherries as well as in fruit that was produced by 2-year-old short spurs or by Ca-treated buds. Differences in the sweet cherry skin metabolic profiles were identified. Fruit produced from Ca-exposed spurs exhibited lower levels of ribose and other cell-wall-related sugars and higher sucrose, maltose, and quininic acid levels. Nutrient shift was increased in red cherries, while anthocyanins were boosted in the black ones. PCA analysis was performed between the high dose of calcium spray and a control for mineral element content and cherry quality traits. This study illustrates that the high dose of calcium application during bud dormancy can effectively improve sweet cherry fruit characteristics, in terms of calcium content, cracking incidence, and fruit set. Overall, the present study contributes to a better understanding of the impact of calcium nutrition in fruit crops, which will provide references for alternative nutrient management and quality control in sweet cherry production.

ACS Style

Michail Michailidis; Chrysanthi Polychroniadou; Maria-Anastasia Kosmidou; Dafni Petraki-Katsoulaki; Evangelos Karagiannis; Athanassios Molassiotis; Georgia Tanou. An Early Calcium Loading during Cherry Tree Dormancy Improves Fruit Quality Features at Harvest. Horticulturae 2021, 7, 135 .

AMA Style

Michail Michailidis, Chrysanthi Polychroniadou, Maria-Anastasia Kosmidou, Dafni Petraki-Katsoulaki, Evangelos Karagiannis, Athanassios Molassiotis, Georgia Tanou. An Early Calcium Loading during Cherry Tree Dormancy Improves Fruit Quality Features at Harvest. Horticulturae. 2021; 7 (6):135.

Chicago/Turabian Style

Michail Michailidis; Chrysanthi Polychroniadou; Maria-Anastasia Kosmidou; Dafni Petraki-Katsoulaki; Evangelos Karagiannis; Athanassios Molassiotis; Georgia Tanou. 2021. "An Early Calcium Loading during Cherry Tree Dormancy Improves Fruit Quality Features at Harvest." Horticulturae 7, no. 6: 135.

Review
Published: 28 May 2021 in International Journal of Molecular Sciences
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The olive tree (Olea europaea L. subsp. europaea) is the most important perennial crop in the Mediterranean region, producing table olives and oil, both appreciated for their nutraceutical value. Although olive oil quality traits have been extensively studied, much less attention has been paid to olive drupe. Olive drupe ripening is an extremely complex process involving numerous physiological and molecular changes that are unique in this fruit crop species. This review underlines the contribution of “-omics” techniques and of the recent advances in bioinformatics and analytical tools, notably next-generation sequencing and mass spectrometry, for the characterization of the olive ripening syndrome. The usage of high-dimensional datasets, such as transcriptomics, proteomics, and metabolomics, will provide a systematical description of the molecular-specific processes regulating olive fruit development and ripening. However, the incomplete sequence of the O. europaea L. reference genome has largely hampered the utilization of omics tools towards olive drupe research. Due to this disadvantage, the most reported -omics studies on fruit trees concern metabolomics and only a few transcriptomics and proteomics. In this review, up-to-date applications of -omics technologies towards olive drupe biology are addressed, and future perspectives in olive fruit research are highlighted.

ACS Style

Christina Skodra; Vaia Titeli; Michail Michailidis; Christos Bazakos; Ioannis Ganopoulos; Athanassios Molassiotis; Georgia Tanou. Olive Fruit Development and Ripening: Break on through to the “-Omics” Side. International Journal of Molecular Sciences 2021, 22, 5806 .

AMA Style

Christina Skodra, Vaia Titeli, Michail Michailidis, Christos Bazakos, Ioannis Ganopoulos, Athanassios Molassiotis, Georgia Tanou. Olive Fruit Development and Ripening: Break on through to the “-Omics” Side. International Journal of Molecular Sciences. 2021; 22 (11):5806.

Chicago/Turabian Style

Christina Skodra; Vaia Titeli; Michail Michailidis; Christos Bazakos; Ioannis Ganopoulos; Athanassios Molassiotis; Georgia Tanou. 2021. "Olive Fruit Development and Ripening: Break on through to the “-Omics” Side." International Journal of Molecular Sciences 22, no. 11: 5806.

Journal article
Published: 17 April 2021 in Horticulturae
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The nutritional value of the peeled and unpeeled fruit (peel plus flesh tissues) was studied using four peach (Prunus persica L.; Red Heaven, Maria Blanca, Big Top, and Queen Giant), two pear (Pyrus communis L.; Santa Maria, Pyrus pyrifolia N.; Nashi), and three apple (Malus domestica Borkh.; Gala, Granny Smith, and Red Chief) cultivars. Based on principal components analysis (PCA) models, there was a clear differentiation among the cultivars’ and the peeled fruits’ nutritional characteristics in comparison to the unpeeled ones. Increased antioxidant capacity and content of total phenols and flavonoids of peaches (Red Heaven and Maria Blanca) versus nectarines (Big Top and Queen Giant) were recorded. In contrast, nectarines were characterized by higher hydroxycinnamates and dry matter. The apples’ cultivar Granny Smith exhibited a high level of titratable acidity (TA), while the Gala displayed a high level of soluble solids concentration (SSC), carotenoids, dry matter, hydroxycinnamic acids, and flavonols at the unpeeled fruit, whereas the Red Chief by increased anthocyanins, antioxidant capacity, total phenols, and flavonoids. Nashi pears with peel were more beneficial due to the strong skin contribution in the fruits’ beneficial compounds content. The peel of the Granny Smith cultivar was associated with an increased level of P, K, Ca, and Mg, whereas that of Red Chief with increased anthocyanins and Mg content.

ACS Style

Michail Michailidis; Evangelos Karagiannis; Elpida Nasiopoulou; Christina Skodra; Athanassios Molassiotis; Georgia Tanou. Peach, Apple, and Pear Fruit Quality: To Peel or Not to Peel? Horticulturae 2021, 7, 85 .

AMA Style

Michail Michailidis, Evangelos Karagiannis, Elpida Nasiopoulou, Christina Skodra, Athanassios Molassiotis, Georgia Tanou. Peach, Apple, and Pear Fruit Quality: To Peel or Not to Peel? Horticulturae. 2021; 7 (4):85.

Chicago/Turabian Style

Michail Michailidis; Evangelos Karagiannis; Elpida Nasiopoulou; Christina Skodra; Athanassios Molassiotis; Georgia Tanou. 2021. "Peach, Apple, and Pear Fruit Quality: To Peel or Not to Peel?" Horticulturae 7, no. 4: 85.

Journal article
Published: 02 April 2021 in Agronomy
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Sweet cherry germplasm contains a high variety of phenotypes which are associated with fruit size and shape as well as sugar content, etc. High phenotypic variation can be a result of genetic or epigenetic diversity that may interact through time. Recent studies have provided evidence that besides allelic variation, epiallelic variation can establish new heritable phenotypes. Herein we conducted a genetic and an epigenetic study (using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) markers, respectively), accompanied by phenotypic traits correlation analysis in sweet cherry gene pools. The mean genetic diversity was greater than the epigenetic diversity (hgen = 0.193; hepi = 0.185), while no significant relationship was found between genetic and epigenetic distance according to a Mantel test. Furthermore, according to correlation analyses our results provided evidence that epigenetic diversity in predefined populations of sweet cherry had a stronger impact on phenotypic traits than their rich genetic diversity.

ACS Style

Evangelia Avramidou; Theodoros Moysiadis; Ioannis Ganopoulos; Michail Michailidis; Christos Kissoudis; Dimitrios Valasiadis; Konstantinos Kazantzis; Eirini Tsaroucha; Athanasios Tsaftaris; Athanassios Molassiotis; Filippos Aravanopoulos; Aliki Xanthopoulou. Phenotypic, Genetic, and Epigenetic Variation among Diverse Sweet Cherry Gene Pools. Agronomy 2021, 11, 680 .

AMA Style

Evangelia Avramidou, Theodoros Moysiadis, Ioannis Ganopoulos, Michail Michailidis, Christos Kissoudis, Dimitrios Valasiadis, Konstantinos Kazantzis, Eirini Tsaroucha, Athanasios Tsaftaris, Athanassios Molassiotis, Filippos Aravanopoulos, Aliki Xanthopoulou. Phenotypic, Genetic, and Epigenetic Variation among Diverse Sweet Cherry Gene Pools. Agronomy. 2021; 11 (4):680.

Chicago/Turabian Style

Evangelia Avramidou; Theodoros Moysiadis; Ioannis Ganopoulos; Michail Michailidis; Christos Kissoudis; Dimitrios Valasiadis; Konstantinos Kazantzis; Eirini Tsaroucha; Athanasios Tsaftaris; Athanassios Molassiotis; Filippos Aravanopoulos; Aliki Xanthopoulou. 2021. "Phenotypic, Genetic, and Epigenetic Variation among Diverse Sweet Cherry Gene Pools." Agronomy 11, no. 4: 680.

Journal article
Published: 26 February 2021 in International Journal of Molecular Sciences
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Drought and heat stresses are major factors limiting crop growth and productivity, and their effect is more devastating when occurring concurrently. Plant glutathione transferases (GSTs) are differentially expressed in response to different stimuli, conferring tolerance to a wide range of abiotic stresses. GSTs from drought-tolerant Phaseolus vulgaris var. “Plake Megalosperma Prespon” is expected to play an important role in the response mechanisms to combined and single heat and drought stresses. Herein, we examined wild-type N. tabacum plants (cv. Basmas Xanthi) and T1 transgenic lines overexpressing the stress-induced Pvgstu3–3 and Pvgstu2–2 genes. The overexpression of Pvgstu3–3 contributed to potential thermotolerance and greater plant performance under combined stress. Significant alterations in the primary metabolism were observed in the transgenic plants between combined stress and stress-free conditions. Stress-responsive differentially expressed genes (DEGs) and transcription factors (TFs) related to photosynthesis, signal transduction, starch and sucrose metabolism, osmotic adjustment and thermotolerance, were identified under combined stress. In contrast, induction of certain DEGs and TF families under stress-free conditions indicated that transgenic plants were in a primed state. The overexpression of the Pvgstu3–3 is playing a leading role in the production of signaling molecules, induction of specific metabolites and activation of the protective mechanisms for enhanced protection against combined abiotic stresses in tobacco.

ACS Style

Evangelia Stavridou; Georgia Voulgari; Michail Michailidis; Stefanos Kostas; Evangelia Chronopoulou; Nikolaos Labrou; Panagiotis Madesis; Irini Nianiou-Obeidat. Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought. International Journal of Molecular Sciences 2021, 22, 2352 .

AMA Style

Evangelia Stavridou, Georgia Voulgari, Michail Michailidis, Stefanos Kostas, Evangelia Chronopoulou, Nikolaos Labrou, Panagiotis Madesis, Irini Nianiou-Obeidat. Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought. International Journal of Molecular Sciences. 2021; 22 (5):2352.

Chicago/Turabian Style

Evangelia Stavridou; Georgia Voulgari; Michail Michailidis; Stefanos Kostas; Evangelia Chronopoulou; Nikolaos Labrou; Panagiotis Madesis; Irini Nianiou-Obeidat. 2021. "Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought." International Journal of Molecular Sciences 22, no. 5: 2352.

Journal article
Published: 08 October 2020 in Food Chemistry
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The current study characterizes the physicochemical, sensory and bioactive compound traits of twenty-two sweet cherry accessions, namely breeding lines, landraces and modern cultivars, embodying the majority of Greek germplasm. The evaluated accessions differ in several quality traits including colour parameters and textural properties as well as sensory attributes, such as taste intensity and overall acceptance. Significant differences in primary metabolites, including fructose, glucose, sorbitol, malic acid were recorded among tested accessions. All genotypes were rich in polyphenols, primarily in quercetin-3,4-O-diglucoside, esculetin, rutin and neochlorogenic acid. An anthocyanins-related discrimination among accessions was also obtained based on cyanidin-3-O-rutinoside and peonidin glycosides content. Overall, the cultivars ‘Tsolakeika’ and ‘Bakirtzeika’ exhibited the higher consumer acceptance while the cultivars ‘Vasiliadi’ and ‘Tragana Edessis-Naousis’ and especially the breeding line ‘TxAg33’ contained high polyphenol levels. These results represent a valuable resource for future breeding efforts for sweet cherry cultivars with improved nutritional quality traits.

ACS Style

Evangelos Karagiannis; Eirini Sarrou; Michail Michailidis; Georgia Tanou; Ioannis Ganopoulos; Christos Bazakos; Konstantinos Kazantzis; Stefan Martens; Aliki Xanthopoulou; Athanassios Molassiotis. Fruit quality trait discovery and metabolic profiling in sweet cherry genebank collection in Greece. Food Chemistry 2020, 342, 128315 .

AMA Style

Evangelos Karagiannis, Eirini Sarrou, Michail Michailidis, Georgia Tanou, Ioannis Ganopoulos, Christos Bazakos, Konstantinos Kazantzis, Stefan Martens, Aliki Xanthopoulou, Athanassios Molassiotis. Fruit quality trait discovery and metabolic profiling in sweet cherry genebank collection in Greece. Food Chemistry. 2020; 342 ():128315.

Chicago/Turabian Style

Evangelos Karagiannis; Eirini Sarrou; Michail Michailidis; Georgia Tanou; Ioannis Ganopoulos; Christos Bazakos; Konstantinos Kazantzis; Stefan Martens; Aliki Xanthopoulou; Athanassios Molassiotis. 2020. "Fruit quality trait discovery and metabolic profiling in sweet cherry genebank collection in Greece." Food Chemistry 342, no. : 128315.

Article
Published: 09 September 2020 in Plant Molecular Biology
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This work provides the first system-wide datasets concerning metabolic changes in calcium-treated fruits, which reveal that exogenously applied calcium may specifically reprogram sweet cherry development and ripening physiognomy. Calcium modulates a wide range of plant developmental processes; however, the regulation of fruit ripening by calcium remains largely uncharacterized. In this study, transcriptome, proteome and metabolome profiling was used to document the responses of sweet cherry fruit to external calcium application (0.5% CaCl2) at 15, 27 and 37 days after full blossom. Endogenous calcium loading in fruit across development following external calcium feeding was accompanied by a reduction in respiration rate. Calcium treatment strongly impaired water-induced fruit cracking tested by two different assays, and this effect depended on the fruit size, water temperature and light/dark conditions. Substantial changes in the levels of numerous polar/non-polar primary and secondary metabolites, including malic acid, glucose, cysteine, epicatechin and neochlorogenic acid were noticed in fruits exposed to calcium. At the onset of ripening, we identified various calcium-affected genes, including those involved in ubiquitin and cysteine signaling, that had not been associated previously with calcium function in fruit biology. Calcium specifically increased the abundance of a significant number of proteins that classified as oxidoreductases, transferases, hydrolases, lyases, and ligases. The overview of temporal changes in gene expression and corresponding protein abundance provided by interlinked analysis revealed that oxidative phosphorylation, hypersensitive response, DNA repair, stomata closure, biosynthesis of secondary metabolites, and proton-pump activity were mainly affected by calcium. This report provides the fullest characterization of expression patterns in calcium-responsive genes, proteins and metabolites currently available in fruit ripening and will serve as a blueprint for future biological endeavors.

ACS Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Martina Samiotaki; George Tsiolas; Eirini Sarrou; George Stamatakis; Ioannis Ganopoulos; Stefans Martens; Anagnostis Argiriou; Athanassios Molassiotis. Novel insights into the calcium action in cherry fruit development revealed by high-throughput mapping. Plant Molecular Biology 2020, 104, 597 -614.

AMA Style

Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Martina Samiotaki, George Tsiolas, Eirini Sarrou, George Stamatakis, Ioannis Ganopoulos, Stefans Martens, Anagnostis Argiriou, Athanassios Molassiotis. Novel insights into the calcium action in cherry fruit development revealed by high-throughput mapping. Plant Molecular Biology. 2020; 104 (6):597-614.

Chicago/Turabian Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Martina Samiotaki; George Tsiolas; Eirini Sarrou; George Stamatakis; Ioannis Ganopoulos; Stefans Martens; Anagnostis Argiriou; Athanassios Molassiotis. 2020. "Novel insights into the calcium action in cherry fruit development revealed by high-throughput mapping." Plant Molecular Biology 104, no. 6: 597-614.

Journal article
Published: 01 August 2020 in Horticulture Research
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Apple (Malus domestica Borkh) is an important fruit crop cultivated in a broad range of environmental conditions. Apple fruit ripening is a physiological process, whose molecular regulatory network response to different environments is still not sufficiently investigated and this is particularly true of the peel tissue. In this study, the influence of environmental conditions associated with low (20 m) and high (750 m) altitude on peel tissue ripening was assessed by physiological measurements combined with metabolomic and proteomic analyses during apple fruit development and ripening. Although apple fruit ripening was itself not affected by the different environmental conditions, several key color parameters, such as redness and color index, were notably induced by high altitude. Consistent with this observation, increased levels of anthocyanin and other phenolic compounds, including cyanidin-3-O-galactoside, quercetin-3-O-rhamnoside, quercetin-3-O-rutinoside, and chlorogenic acid were identified in the peel of apple grown at high altitude. Moreover, the high-altitude environment was characterized by elevated abundance of various carbohydrates (e.g., arabinose, xylose, and sucrose) but decreased levels of glutamic acid and several related proteins, such as glycine hydroxymethyltransferase and glutamate–glyoxylate aminotransferase. Other processes affected by high altitude were the TCA cycle, the synthesis of oxidative/defense enzymes, and the accumulation of photosynthetic proteins. From the obtained data we were able to construct a metabolite-protein network depicting the impact of altitude on peel ripening. The combined analyses presented here provide new insights into physiological processes linking apple peel ripening with the prevailing environmental conditions.

ACS Style

Evangelos Karagiannis; Michail Michailidis; Georgia Tanou; Federico Scossa; Eirini Sarrou; George Stamatakis; Martina Samiotaki; Stefan Martens; Alisdair R. Fernie; Athanassios Molassiotis. Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening. Horticulture Research 2020, 7, 1 -12.

AMA Style

Evangelos Karagiannis, Michail Michailidis, Georgia Tanou, Federico Scossa, Eirini Sarrou, George Stamatakis, Martina Samiotaki, Stefan Martens, Alisdair R. Fernie, Athanassios Molassiotis. Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening. Horticulture Research. 2020; 7 (1):1-12.

Chicago/Turabian Style

Evangelos Karagiannis; Michail Michailidis; Georgia Tanou; Federico Scossa; Eirini Sarrou; George Stamatakis; Martina Samiotaki; Stefan Martens; Alisdair R. Fernie; Athanassios Molassiotis. 2020. "Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening." Horticulture Research 7, no. 1: 1-12.

Journal article
Published: 01 May 2020 in Horticulture Research
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Sweet cherries, Prunus avium L. (Rosaceae), are gaining importance due to their perenniallity and nutritional attributes beneficial for human health. Interestingly, sweet cherry cultivars exhibit a wide range of phenotypic diversity in important agronomic traits, such as flowering time and defense reactions against pathogens. In this study, whole-genome resequencing (WGRS) was employed to characterize genetic variation, population structure and allelic variants in a panel of 20 sweet cherry and one wild cherry genotypes, embodying the majority of cultivated Greek germplasm and a representative of a local wild cherry elite phenotype. The 21 genotypes were sequenced in an average depth of coverage of 33.91×. and effective mapping depth, to the genomic reference sequence of ‘Satonishiki’ cultivar, between 22.21× to 36.62×. Discriminant analysis of principal components (DAPC) with SNPs revealed two clusters of genotypes. There was a rapid linkage disequilibrium decay, as the majority of SNP pairs with r 2 in near complete disequilibrium (>0.8) were found at physical distances less than 10 kb. Functional analysis of the variants showed that the genomic ratio of non-synonymous/synonymous (dN/dS) changes was 1.78. The higher dN frequency in the Greek cohort of sweet cherry could be the result of artificial selection pressure imposed by breeding, in combination with the vegetative propagation of domesticated cultivars through grafting. The majority of SNPs with high impact (e.g., stop codon gaining, frameshift), were identified in genes involved in flowering time, dormancy and defense reactions against pathogens, providing promising resources for future breeding programs. Our study has established the foundation for further large scale characterization of sweet cherry germplasm, enabling breeders to incorporate diverse germplasm and allelic variants to fine tune flowering and maturity time and disease resistance in sweet cherry cultivars.

ACS Style

Aliki Xanthopoulou; Maria Manioudaki; Christos Bazakos; Christos Kissoudis; Anna-Maria Farsakoglou; Evangelos Karagiannis; Michail Michailidis; Chrysanthi Polychroniadou; Antonios Zambounis; Konstantinos Kazantzis; Athanasios Tsaftaris; Panagiotis Madesis; Filippos Aravanopoulos; Athanassios Molassiotis; Ioannis Ganopoulos. Whole genome re-sequencing of sweet cherry (Prunus avium L.) yields insights into genomic diversity of a fruit species. Horticulture Research 2020, 7, 1 -14.

AMA Style

Aliki Xanthopoulou, Maria Manioudaki, Christos Bazakos, Christos Kissoudis, Anna-Maria Farsakoglou, Evangelos Karagiannis, Michail Michailidis, Chrysanthi Polychroniadou, Antonios Zambounis, Konstantinos Kazantzis, Athanasios Tsaftaris, Panagiotis Madesis, Filippos Aravanopoulos, Athanassios Molassiotis, Ioannis Ganopoulos. Whole genome re-sequencing of sweet cherry (Prunus avium L.) yields insights into genomic diversity of a fruit species. Horticulture Research. 2020; 7 (1):1-14.

Chicago/Turabian Style

Aliki Xanthopoulou; Maria Manioudaki; Christos Bazakos; Christos Kissoudis; Anna-Maria Farsakoglou; Evangelos Karagiannis; Michail Michailidis; Chrysanthi Polychroniadou; Antonios Zambounis; Konstantinos Kazantzis; Athanasios Tsaftaris; Panagiotis Madesis; Filippos Aravanopoulos; Athanassios Molassiotis; Ioannis Ganopoulos. 2020. "Whole genome re-sequencing of sweet cherry (Prunus avium L.) yields insights into genomic diversity of a fruit species." Horticulture Research 7, no. 1: 1-14.

Journal article
Published: 10 April 2020 in Plant Methods
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Background Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is commonly used; however, this test does not adequately simulate environmental conditions. Herein, we have designed and evaluated a cracking protocol, named ‘Waterfall method’, in which fruits are continuously wetted under controlled conditions. Results The application of this method alone, or in combination with ‘Christensen method, was shown to be a reliable approach to characterize sweet cherry cracking behavior. Seventeen cherry cultivars were tested for their cracking behavior using both protocols, and primary as well as secondary metabolites identification was performed in skin tissue using a combined GC–MS and UPLC-MS/MS platform. Significant variations of some of the detected metabolites were discovered and important cracking index–metabolite correlations were identified. Conclusions We have established an alternative/complementary method of cherry cracking characterization alongside to Christiansen assay.

ACS Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Eirini Sarrou; Katerina Karamanoli; Athina Lazaridou; Stefan Martens; Athanassios Molassiotis. Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening. Plant Methods 2020, 16, 1 -14.

AMA Style

Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Eirini Sarrou, Katerina Karamanoli, Athina Lazaridou, Stefan Martens, Athanassios Molassiotis. Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening. Plant Methods. 2020; 16 (1):1-14.

Chicago/Turabian Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Eirini Sarrou; Katerina Karamanoli; Athina Lazaridou; Stefan Martens; Athanassios Molassiotis. 2020. "Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening." Plant Methods 16, no. 1: 1-14.

Journal article
Published: 13 February 2020 in Plant Physiology and Biochemistry
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Despite the application of girdling technique for several centuries, its impact on the metabolic shifts that underly fruit biology remains fragmentary. To characterize the influence of girdling on sweet cherry (Prunus avium L.) fruit development and ripening, second-year-old shoots of the cultivars ‘Lapins’ and ‘Skeena’ were girdled before full blossom. Fruit characteristics were evaluated across six developmental stages (S), from green-small fruit (stage S1) to full ripe stage (stage S6). In both cultivars, girdling significantly altered the fruit ripening physiognomy. Time course fruit metabolomic along with proteomic approaches unraveled common and cultivar-specific responses to girdling. Notably, several primary and secondary metabolites, such as soluble sugars (glucose, trehalose), alcohol (mannitol), phenolic compounds (rutin, naringenin-7-O-glucoside), including anthocyanins (cyanidin-3-O-rutinoside, cyanidin-3-O-galactoside, cyanidin-3.5-O-diglucoside) were accumulated by girdling, while various amino acids (glycine, threonine, asparagine) were decreased in both cultivars. Proteins predominantly associated with ribosome, DNA repair and recombination, chromosome, membrane trafficking, RNA transport, oxidative phosphorylation, and redox homeostasis were depressed in fruits of both girdled cultivars. This study provides the first system-wide datasets concerning metabolomic and proteomic changes in girdled fruits, which reveal that shoot girdling may induce long-term changes in sweet cherry metabolism.

ACS Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Martina Samiotaki; Eirini Sarrou; Katerina Karamanoli; Athina Lazaridou; Stefan Martens; Athanassios Molassiotis. Proteomic and metabolic analysis reveals novel sweet cherry fruit development regulatory points influenced by girdling. Plant Physiology and Biochemistry 2020, 149, 233 -244.

AMA Style

Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Martina Samiotaki, Eirini Sarrou, Katerina Karamanoli, Athina Lazaridou, Stefan Martens, Athanassios Molassiotis. Proteomic and metabolic analysis reveals novel sweet cherry fruit development regulatory points influenced by girdling. Plant Physiology and Biochemistry. 2020; 149 ():233-244.

Chicago/Turabian Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Martina Samiotaki; Eirini Sarrou; Katerina Karamanoli; Athina Lazaridou; Stefan Martens; Athanassios Molassiotis. 2020. "Proteomic and metabolic analysis reveals novel sweet cherry fruit development regulatory points influenced by girdling." Plant Physiology and Biochemistry 149, no. : 233-244.

Original research article
Published: 13 February 2020 in Frontiers in Plant Science
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Superficial scald is a major physiological disorder in apple fruit that is induced by cold storage and is mainly expressed as brown necrotic patches on peel tissue. However, a global view of the gene-protein-metabolite interactome underlying scald prevention/sensitivity is currently missing. Herein, we have found for the first time that cold storage in an atmosphere enriched with ozone (O3) induced scald symptoms in ‘Granny Smith’ apple fruits during subsequent ripening at room temperature. In contrast, treatment with the ethylene perception inhibitor 1-methylcyclopropene (1-MCP) reversed this O3-induced scald effect. Amino acids, including branched-chain amino acids, were the most strongly induced metabolites in peel tissue of 1-MCP treated fruits. Proteins involved in oxidative stress and protein trafficking were differentially accumulated prior to and during scald development. Genes involved in photosynthesis, flavonoid biosynthesis and ethylene signaling displayed significant alterations in response to 1-MCP and O3. Analysis of regulatory module networks identified putative transcription factors (TFs) that could be involved in scald. Subsequently, a transcriptional network of the genes-proteins-metabolites and the connected TFs was constructed. This approach enabled identification of several genes coregulated by TFs, notably encoding glutathione S-transferase (GST) protein(s) with distinct signatures following 1-MCP and O3 treatments. Overall, this study is an important contribution to future functional studies and breeding programs for this fruit, aiding to the development of improved apple cultivars to superficial scald.

ACS Style

Evangelos Karagiannis; Georgia Tanou; Federico Scossa; Martina Samiotaki; Michail Michailidis; Maria Manioudaki; François Laurens; Dominique Job; Alisdair R. Fernie; Mathilde Orsel; Athanassios Molassiotis. Systems-Based Approaches to Unravel Networks and Individual Elements Involved in Apple Superficial Scald. Frontiers in Plant Science 2020, 11, 1 .

AMA Style

Evangelos Karagiannis, Georgia Tanou, Federico Scossa, Martina Samiotaki, Michail Michailidis, Maria Manioudaki, François Laurens, Dominique Job, Alisdair R. Fernie, Mathilde Orsel, Athanassios Molassiotis. Systems-Based Approaches to Unravel Networks and Individual Elements Involved in Apple Superficial Scald. Frontiers in Plant Science. 2020; 11 ():1.

Chicago/Turabian Style

Evangelos Karagiannis; Georgia Tanou; Federico Scossa; Martina Samiotaki; Michail Michailidis; Maria Manioudaki; François Laurens; Dominique Job; Alisdair R. Fernie; Mathilde Orsel; Athanassios Molassiotis. 2020. "Systems-Based Approaches to Unravel Networks and Individual Elements Involved in Apple Superficial Scald." Frontiers in Plant Science 11, no. : 1.

Book chapter
Published: 24 January 2020 in Fruit Crops
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The current chapter provides an overview toward the potential effect of foliar treated nutrients (macro or micro) upon the quality characteristics of various fruits. Through the prism of fruit quality, the unique role of each macro- or micronutrients is presented, along with the direct effect that they exert toward external or internal fruit quality attributes. Furthermore, several scientific data present the ability of the foliar application of nutrients to be used as an alternative method for the improvement of several quality markers. Potential interplay or potent parallel cascades among nutrients are presented, which can contribute positively and facilitate the assimilation and distribution of necessary nutrients for a normal fruit development without deformations or external defects.

ACS Style

Vasileios Ziogas; Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Athanassios Molassiotis. Manipulating fruit quality through foliar nutrition. Fruit Crops 2020, 401 -417.

AMA Style

Vasileios Ziogas, Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Athanassios Molassiotis. Manipulating fruit quality through foliar nutrition. Fruit Crops. 2020; ():401-417.

Chicago/Turabian Style

Vasileios Ziogas; Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Athanassios Molassiotis. 2020. "Manipulating fruit quality through foliar nutrition." Fruit Crops , no. : 401-417.

Journal article
Published: 13 November 2019 in Industrial Crops and Products
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Tobacco is an economically important crop with leaves that are a significant source of aromatic, bioactive compounds such as phenolics and alkaloids. In the present study un-biased transcriptomics and metabolomics approaches were employed to identify and quantify individual changes in transcript and metabolite profiles in leaves of three oriental tobacco varieties. Based on next generation sequencing (NGS) and gas chromatography mass spectrometry (GC–MS) technologies, a wide variety of transcripts and metabolites was detected and the metabolic diversity among varieties was determined. Genes with largest expression differences were identified in the leaves of the three varieties; among them three were commonly over-expressed in two varieties in comparison with the third variety. Notably, significant expression differences were recorded in phenylalanine ammonia lyase (PAL) genes that are key genes of phenylpropanoid biosynthesis. Following transcriptomics, metabolomics analysis has shown that polyphenolic compounds varied widely among the three varieties. Furthermore, statistically significant differences in soluble sugars, alcohols, organic acids, amino acids and other metabolites were also revealed. The integration of the two -omics datasets in determining diversity of tobacco varieties offers important readouts for the genetic control of metabolite production and constitutes a resource for future studies in the area of plant biotechnology for improving tobacco specific traits.

ACS Style

Aphrodite Tsaballa; Eirini Sarrou; Aliki Xanthopoulou; Eleni Tsaliki; Christos Kissoudis; Evangelos Karagiannis; Michail Michailidis; Stefan Martens; Elektra Sperdouli; Zoe Hilioti; Vasileios Fotopoulos; Irini Nianiou-Obeidat; Athanasios Tsaftaris; Panagiotis Madesis; Apostolos Kalivas; Ioannis Ganopoulos. Comprehensive approaches reveal key transcripts and metabolites highlighting metabolic diversity among three oriental tobacco varieties. Industrial Crops and Products 2019, 143, 111933 .

AMA Style

Aphrodite Tsaballa, Eirini Sarrou, Aliki Xanthopoulou, Eleni Tsaliki, Christos Kissoudis, Evangelos Karagiannis, Michail Michailidis, Stefan Martens, Elektra Sperdouli, Zoe Hilioti, Vasileios Fotopoulos, Irini Nianiou-Obeidat, Athanasios Tsaftaris, Panagiotis Madesis, Apostolos Kalivas, Ioannis Ganopoulos. Comprehensive approaches reveal key transcripts and metabolites highlighting metabolic diversity among three oriental tobacco varieties. Industrial Crops and Products. 2019; 143 ():111933.

Chicago/Turabian Style

Aphrodite Tsaballa; Eirini Sarrou; Aliki Xanthopoulou; Eleni Tsaliki; Christos Kissoudis; Evangelos Karagiannis; Michail Michailidis; Stefan Martens; Elektra Sperdouli; Zoe Hilioti; Vasileios Fotopoulos; Irini Nianiou-Obeidat; Athanasios Tsaftaris; Panagiotis Madesis; Apostolos Kalivas; Ioannis Ganopoulos. 2019. "Comprehensive approaches reveal key transcripts and metabolites highlighting metabolic diversity among three oriental tobacco varieties." Industrial Crops and Products 143, no. : 111933.

Journal article
Published: 19 September 2019 in Plant Physiology and Biochemistry
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The impact of ultraviolet-C (UV–C) irradiation on sweet cherry fruit was studied. Following harvest, fruits (cv. Sweetheart) were exposed to different doses of UV-C (0, 1.2, 3.0 or 6.0 kJ m−2) and then cold stored (0 °C) for 10 days. Treatments with UV-C delayed most ripening features and reduced pitting symptoms, particularly following prolonged UV-C application. Also, application of the highest UV-C dose inhibited pectin degradation and delayed skin resistance to penetration. An activation of antioxidants capacity and bioactive compounds, such as flavonoids and phenolics was observed. Illumination with UV-C diminished respiration and altered metabolite profile in whole fruit and skin samples. Several amino acids (eg., threonine and aspartate), sugars, (eg., glucose and fructose) and alcohols (e.g., inositol and mannitol) were modulated by long-term UV-C treatment in whole cherry fruit. Various metabolites, including malate, galacturonate, oxoproline and glutamine were also modulated by UV-C skin tissue. These data enhance our understanding of UV-C function in fruit biology.

ACS Style

Michail Michailidis; Evangelos Karagiannis; Chrysanthi Polychroniadou; Georgia Tanou; Katerina Karamanoli; Athanassios Molassiotis. Metabolic features underlying the response of sweet cherry fruit to postharvest UV-C irradiation. Plant Physiology and Biochemistry 2019, 144, 49 -57.

AMA Style

Michail Michailidis, Evangelos Karagiannis, Chrysanthi Polychroniadou, Georgia Tanou, Katerina Karamanoli, Athanassios Molassiotis. Metabolic features underlying the response of sweet cherry fruit to postharvest UV-C irradiation. Plant Physiology and Biochemistry. 2019; 144 ():49-57.

Chicago/Turabian Style

Michail Michailidis; Evangelos Karagiannis; Chrysanthi Polychroniadou; Georgia Tanou; Katerina Karamanoli; Athanassios Molassiotis. 2019. "Metabolic features underlying the response of sweet cherry fruit to postharvest UV-C irradiation." Plant Physiology and Biochemistry 144, no. : 49-57.

Original article
Published: 17 September 2019 in Planta
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Ηeat and calcium treatments reprogram sweet cherry fruit metabolism during postharvest senescence as evidenced by changes in respiration, amino acid metabolism, sugars, and secondary metabolites shift. Heat and calcium treatments are used to improve postharvest fruit longevity; however, the exact mechanism remains poorly understood. To characterize the impact of these treatments on sweet cherries metabolism, ‘Lapins’ fruits were treated with heat or CaCl2 solutions and their combination and subsequently were exposed at room temperature, for up to 4 days, defined as senescence period. Single and combined heat and calcium treatments partially delayed fruit senescence, as evidenced by changes in fruit colour darkening, skin penetration force, and respiration activity. Calcium content was noticeably increased by heat in Ca-treated fruit. Several primary metabolites, including amino acids, organic acids, and alcohols, were decreased in response to both treatments, while many soluble sugars and secondary metabolites were increased within 1 day post-treatment. Changes of several metabolites in heat-treated fruits, especially esculetin, peonidin 3-O-glucoside and peonidin 3-O-galactoside, ribose, pyroglutamate, and isorhamnetin-3-O-rutinoside, were detected. The metabolome of fruit exposed to calcium also displayed substantial modulations, particularly in the levels of galactose, glycerate, aspartate, tryptophan, phospharate rutin, and peonidin 3-O-glucoside. The expression of several genes involved in TCA cycle (MDH1, IDH1, OGDH, SUCLA2, and SDH1-1), pectin degradation (ADPG1) as well as secondary (SK1, 4CL1, HCT, and BAN), amino acids (ALDH18A1, ALDH4A1, GS, GAD, GOT2, OPLAH, HSDH, and SDS), and sugar (PDHA1 and DLAT) metabolism were affected by both treatments. Pathway-specific analysis further revealed the regulation of fruit metabolic programming by heat and calcium. This work provides a comprehensive understanding of metabolic regulation in response to heat and calcium during fruit senescence.

ACS Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Eirini Sarrou; Evangelia Stavridou; Ioannis Ganopoulos; Katerina Karamanoli; Panagiotis Madesis; Stefan Martens; Athanassios Molassiotis. An integrated metabolomic and gene expression analysis identifies heat and calcium metabolic networks underlying postharvest sweet cherry fruit senescence. Planta 2019, 250, 2009 -2022.

AMA Style

Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Eirini Sarrou, Evangelia Stavridou, Ioannis Ganopoulos, Katerina Karamanoli, Panagiotis Madesis, Stefan Martens, Athanassios Molassiotis. An integrated metabolomic and gene expression analysis identifies heat and calcium metabolic networks underlying postharvest sweet cherry fruit senescence. Planta. 2019; 250 (6):2009-2022.

Chicago/Turabian Style

Michail Michailidis; Evangelos Karagiannis; Georgia Tanou; Eirini Sarrou; Evangelia Stavridou; Ioannis Ganopoulos; Katerina Karamanoli; Panagiotis Madesis; Stefan Martens; Athanassios Molassiotis. 2019. "An integrated metabolomic and gene expression analysis identifies heat and calcium metabolic networks underlying postharvest sweet cherry fruit senescence." Planta 250, no. 6: 2009-2022.

Original research article
Published: 11 January 2019 in Frontiers in Plant Science
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Chloroplasts are organelles subjected to extreme oxidative stress conditions. Biomolecules produced in the chloroplasts act as signals guiding plant metabolism toward stress tolerance and play a major role in regulating gene expression in the nucleus. Herein, we used transplastomic plants as an alternative approach to expression of transgenes in the nucleus for conferring stress tolerance to abiotic stresses and herbicides. To investigate the morphophysiological and molecular mechanisms and the role of plastid expressed GSTs in tobacco stress detoxification and stress tolerance, we used transplastomic tobacco lines overexpressing a theta class glutathione transferase (GST) in chloroplasts. The transplastomic plants were tested under drought (0, 100, and 200 mM mannitol) and salinity (0, 150, and 300 mM NaCl) in vitro, and under herbicide stress (Diquat). Our results suggest that ptAtGSTT lines were tolerant to herbicide-induced oxidative and salinity stresses and showed enhanced response tolerance to mannitol-induced osmotic stress compared to WT plants. Overexpression of the Arabidopsis thaliana AtGSTT in the chloroplasts resulted in enhanced photo-tolerance and turgor maintenance under stress. Whole-genome transcriptome analysis revealed that genes related to stress tolerance, were upregulated in ptAtGSTT2a line under both control and high mannitol stress conditions. Transplastomic plants overexpressing the ptAtGSTT2a in the chloroplast showed a state of acclimation to stress, as only limited number of genes were upregulated in the ptAtGSTT2a transplastomic line compared to WT under stress conditions while at the same time genes related to stress tolerance were upregulated in ptAtGSTT2a plants compared to WT in stress-free conditions. In parallel, the metabolic profile indicated limited perturbations of the metabolic homeostasis in the transplastomic lines and greater accumulation of mannitol, and soluble sugars under high mannitol stress. Therefore, transplastomic lines seem to be in a state of acclimation to stress under stress-free conditions, which was maintained even under high mannitol stress. The results help to elucidate the role of GSTs in plant abiotic stress tolerance and the underlying mechanisms of the GSTs expressed in the chloroplast, toward environmental resilience of cultivated crops.

ACS Style

Evangelia Stavridou; Michail Michailidis; Stella Gedeon; Antri Ioakeim; Stefanos Kostas; Evangelia Chronopoulou; Nikolaos E. Labrou; Robert Edwards; Anil Day; Irini Nianiou-Obeidat; Panagiotis Madesis. Tolerance of Transplastomic Tobacco Plants Overexpressing a Theta Class Glutathione Transferase to Abiotic and Oxidative Stresses. Frontiers in Plant Science 2019, 9, 1861 .

AMA Style

Evangelia Stavridou, Michail Michailidis, Stella Gedeon, Antri Ioakeim, Stefanos Kostas, Evangelia Chronopoulou, Nikolaos E. Labrou, Robert Edwards, Anil Day, Irini Nianiou-Obeidat, Panagiotis Madesis. Tolerance of Transplastomic Tobacco Plants Overexpressing a Theta Class Glutathione Transferase to Abiotic and Oxidative Stresses. Frontiers in Plant Science. 2019; 9 ():1861.

Chicago/Turabian Style

Evangelia Stavridou; Michail Michailidis; Stella Gedeon; Antri Ioakeim; Stefanos Kostas; Evangelia Chronopoulou; Nikolaos E. Labrou; Robert Edwards; Anil Day; Irini Nianiou-Obeidat; Panagiotis Madesis. 2019. "Tolerance of Transplastomic Tobacco Plants Overexpressing a Theta Class Glutathione Transferase to Abiotic and Oxidative Stresses." Frontiers in Plant Science 9, no. : 1861.