This study assessed the effects of leukocyte-platelet-rich fibrin (L–PRF) on soft tissue healing and the correlation with the local concentration of growth factors (GF) and cytokines in the dental socket of lower third molars. Forty lower-third molars (20 participants) were included in this randomized, double-blinded, split-mouth study. After extractions, randomized sides received alveolar filling with L–PRF on one side and a natural blood clot on the other side. The pain was assessed for up to seven days and soft tissue healing (Landry index) for 14 days post-extraction. Swabs were collected from the surgical sites for GF and cytokine assessment by flow luminometry. Participants reported lower postoperative pain on the sides grafted with L–PRF, which also presented increased tissue healing scores (p < 0.05). There were increased levels of all GFs and several cytokines at the L–PRF site at day one, while vascular endothelial growth factor (VEGF), IL–10, and IL1–RA remained higher throughout for seven days (p < 0.05). VEGF concentration at L–PRF sites correlated positively with the participants’ blood platelet content (ρ = 0.769). PDGF correlated negatively with pain experience on days 2 and 3, and positively with soft tissue healing scores, while FGFb presented a weak correlation with a reduction of pain on day 3. The use of L–PRF improves the soft tissue healing process and decreases postoperative pain after the third molar extractions, which correlates with an increase in the local concentration of growth factors such as PDGF and FGFb.

Bone tissue engineering seeks biomaterials that enable cell migration, angiogenesis, matrix deposition, and tissue regeneration. Blood concentrates like platelet-rich fibrin (L-PRF) offer a cost-effective source of cells and growth factors to enhance healing. The present study aimed to evaluate heated serum albumin with liquid PRF (Alb-PRF) and L-PRF clinically and biochemically after placement in dental sockets following mandibular third molar extraction. In a controlled, split-mouth study involving 10 volunteers, 20 extracted molars were treated with either Alb-PRF or L-PRF. Post-extraction, pain, trismus, infection presence, and swelling were measured. The concentrations of different analytes in the surgical sites were also examined. The data were statistically analyzed, with significance defined at p < 0.05 (t-test). No significant difference was noted between the groups for pain and trismus, but Alb-PRF showed a significant reduction in swelling on day seven. The Alb-PRF group showed lower levels of pro-inflammatory cytokines (GM-CSF, IL-1b, IL-6, IFNy, IL-8, IL-15, RANTES, and MIP-1a) after seven days, with only higher expressions of MIP-1b, IL-1b, and MCP-1 found in the L-PRF group. Differences were observed in the release of analytes between L-PRF and Alb-PRF, with Alb-PRF significantly reducing edema after seven days. Alb-PRF reduced edema, while L-PRF increased inflammatory cytokines. When compared to L-PRF, Alb-PRF reduced edema and the release of inflammatory cytokines, suggesting promising effects in socket healing while underscoring the role of growth factors and cytokines in potential applications of blood concentrates.

RESUMO As lesões dentárias traumáticas em pacientes pediátricos são condições relativamente comuns na Odontologia. A avaliação e o diagnóstico corretos são determinantes para o sucesso do tratamento a longo prazo. Este relato descreve o caso de uma paciente pediátrica que sofreu uma queda da própria altura que ocasionou luxação lateral associada a luxação intrusiva, além de fratura da tábua óssea alveolar vestibular e fratura coronária dos elementos envolvidos, exigindo um manejo clínico delicado e efetivo. Em dois anos de acompanhamento a paciente não apresentou nenhuma complicação, exibindo resultados clínicos satisfatórios com a conduta terapêutica aplicada. Palavras-chave: Traumatismo dentário, emergências, osso, dentes, periodontal. ABSTRACTTraumatic dental injuries in pediatric patients are relatively common conditions in dentistry. Correct medical evaluation and diagnosis are critical to long-term treatment success. This report describes the case of a pediatric patient who suffered a fall from his own height that caused lateral dislocation associated with intrusive dislocation, in addition to fracture of the vestibular alveolar bone plate and coronary fracture of the involved elements requiring a delicate and effective clinical management.In two years of follow-up, the patient did not present any complications, showing satisfactory clinical results with the applied therapeutic approach. Key words: Dental trauma, emergencies, bone, teeth, periodontal.