Coexistence between species with similar ecological niches implies species must segregate along one or more niche axes to survive. Space, time, and trophic resources are regarded as the principal axes upon which species segregate. We examined segregation along these niche axes to determine mechanisms underlying coexistence between the two main predators, puma (Puma concolor) and culpeo foxes (Lycalopex culpaeus) in the Andes of Central Chile. We used occupancy modeling to examine space use and overlap, Kernel Density Estimation to determine temporal activity patterns and overlap, and analysis of prey remains in feces to assess diet breadth and similarity. We found high spatial overlap and positive associations between detection of the carnivores lending little support for spatial segregation. Similarly, we found high nocturnal, temporal overlap between pumas and foxes that matched peaks in activity of prey. In contrast, we found relatively low dietary overlap indicating niche segregation likely occurs along the dietary axis. The Puma diet was dominated by introduced, exotic hares and foxes appeared to shift away from hares to rabbits, small mammals, and seeds. Given that lagomorphs are the main dietary resource for pumas in particular, management decisions regarding the control or eradication of such exotic species could negatively affected puma survival.

Ecologically based rodent management strategies are arising as a sustainable approach to rodent control, allowing us to preserve biodiversity while safeguarding human economic activities. Despite predator signals being known to generally repel rodents, few field-based studies have compared the behavioral effects of several predators on different prey species, especially in Neotropical ecosystems. Here, we used camera traps to study the behavior of rodent species native to the Chilean temperate forest (Abrothrix spp., long-tailed pygmy rice rat Oligoryzomys longicaudatus) and an introduced rodent (black rat Rattus rattus). Using playbacks of raptor calls, we experimentally exposed rodents to three predation risk treatments: austral pygmy owl calls (Glaucidium nana), rufous-legged owl calls (Strix rufipes) and a control treatment (absence of owl calls). We evaluated the effects of the treatments on the time allocated to three behaviors: feeding time, locomotor activity and vigilance. Moonlight and vegetation cover were also considered in the analyses, as they can modify perceived predation risk. Results showed that predator calls and environmental factors modified prey behavior depending not only on the predator species, but also on the rodent species. Consequently, owl playbacks could be regarded as a promising rodent control tool, knowing that future studies would be critical to deeply understand differences between species in order to select the most effective predator cues.