Composting is described as a sustainable alternative to organic waste reuse from the agricultural and household sectors. The organic matter degradation and stabilization product presents great variability due to the waste composition used. Thus, the use of techniques that allow the monitoring of the decomposition process is necessary to determine the quality of the final product. Therefore, this review critically analyzes and updates the chemical spectroscopy methods described for the evaluation of the organic matter transformation in the composting process to optimize and generate amendments for agricultural use. This review examines spectroscopic techniques such as ultraviolet-visible (UV-Vis), infrared (IR), fluorescence, and 13C NMR reported for the evolution and maturation of carbonate structures in composting. In addition, they are used in the study of indicators for monitoring the degradation of complex structures, such as sugars and proteins, for the formation of precursors that are responsible for the formation of highly stabilized substances such as humic and fulvic compounds. Finally, these parameters may be used to elucidate organic matter degradation and its stabilization process, establish patterns that characterize each stage of composting along with its physicochemical characteristics, and monitor potential phytotoxicity levels.

Agroforestry systems (AFSs) have gained recognition as a land use strategy to address food security and climate change. They involve intentionally cultivating trees alongside crops and/or animals. AFSs cover approximately 5% of the global forest area and promote sustainable soil conservation, including soil organic carbon (C) sequestration (C_SEQ). In some areas of Chile, AFSs are used to preserve the ecological value of native forests. This study evaluates the effects of two AFSs, namely, an agroforest for fodder production (A_GROF_RST) and Silvopastoral (SPS), within a degraded native forest (Nothofagus obliqua sp.). The evaluation focuses on their impact on C_SEQ capacity and soil quality (SQ), using soil quality indexes (SQIs) derived from 30 soil quality indicators (SI_NDs) related to physical, chemical, and microbiological properties at two depths (0–5 and 5–20 cm). The results for the total depth analyzed (0–20 cm) indicate an average C_SEQ of 6.88 and 4.83 Mg C yr⁻¹ and a global SQI of 37.8% and 31.0% for A_GROF_RST and SPS, respectively. Among the thirteen SI_NDs that demonstrated significant differences (p < 0.05), five were associated with the considered depths (P⁺, Ca²⁺, S, ECEC, and Al_SAT), three differed between A_GROF_RST and SPS (BD, NH₄⁺, NO₃⁻), while SOC, K⁺, and Mg²⁺ varied across all conditions (e.g., combinations of systems and depths), and β-_GLU and N_MIN differed in a single condition. However, almost all 30 SI_NDs analyzed showed higher values at the 0–5 cm depth, indicating the positive effects of soil organic matter (SOM)/SOC additions. Significant interactions (Pearson’s correlation) revealed that SOC correlated with most SI_NDs (e.g., N, NH₄⁺, P⁺, K⁺, Ca²⁺, Mg²⁺, S, ECEC, N_MIN). These findings suggest that both A_GROF_RST and SPS systems have similar capabilities in restoring the ecological value of native Nothofagus forests while providing conditions for productive and complementary use. This sustainable option offers opportunities for cattle production alongside ecological restoration efforts and provides a possible strategy to generate public policies related to the ecosystem services of agroforestry systems.

Berberis empetrifolia L. and Berberis darwinii H. are native mountain plants of Chile and Argentina, characterised by their benzylisoquinolic alkaloid content. This research aims to analyse benzylisoquinoline alkaloids and their antibacterial activity in root extracts of Berberis empetrifolia L. and Berberis darwinii H. A methanol extract was separated by column chromatography through a polarity gradient and alkaloids were characterised by HPLC ESI-MS/MS. Antibacterial activity against Gram-positive and Gram-negative was assessed. The results showed the presence of protoberberine and aporphine alkaloids in the total extract and chloroform-methanol (CM) fraction. The antibacterial activity reports Staphylococcus aureus and S. epidermidis inhibitions. The berberine content can contribute to strain inhibition due to the synergic effect with other metabolites. A varietal effect could explain the difference in results between the species evaluated. This research generates new visions in the study of endemic species in the search for bioactive compounds of pharmacological interest.

In this work, the antibacterial activity of Laureliopsis philippiana (Looser) essential oil was studied, and its kinetic release performance using different surface-functionalized nanostructured porous silicon (nPSi) was analyzed. Experimental results showed the high inhibitory effect of Laureliopsis philippiana essential oil against Staphylococcus aureus and Klebsiella pneumoniae. In addition, the essential oil was successfully loaded into different kinds of functionalized nPSi. FTIR measurements indicated the formation of stable complexes in the nPSi functionalization process. Specifically, chemical oxidized nPSi (nPSi-Ox), 3-aminopropyltriethoxysilane functionalized nPSi (nPSi-APTS), undecylenic acid-functionalized nPSi (nPSi-UAc), chitosan (nPSi-Chi) and β-cyclodextrin (nPSi-βCD) polymer functionalization on nPSi were studied. nPSi-Ox, nPSi-APTS, and nPSi-UAc were covalent functionalization, and nPSi-Chi and nPSi-βCD were obtained by electrostatic attachment. The kinetic study demonstrated a controlled release of up to 4 h for all the samples following a quasi-Fickian diffusion mechanism. Moreover, the use of functionalized nPSi-APTS and nPSi-UAc structures allows a more controlled kinetic release of Laureliopsis philippiana essential oil in comparison to the rest of the functionalization, increasing its availability and exposure to the environment.