This page has only limited features, please log in for full access.
Deoxynivalenol (DON) is one of the most common mycotoxins in grains, causing gastrointestinal inflammation, neurotoxicity, hepatotoxicity and embryotoxicity, even at a low quantity. In this study, a facile electrochemical aptasensor was established for the rapid and sensitive determination of DON based on a multifunctional N-doped Cu-metallic organic framework (N–Cu–MOF) nanomaterial. The N–Cu–MOF, with a large specific surface area and good electrical conductivity, served not only as an optimal electrical signal probe but also as an effective supporting substrate for stabilizing aptamers through the interactions of amino (-NH2) and copper. Under the optimal conditions, the proposed sensor provided a wide linear concentration range of 0.02–20 ng mL−1 (R2 = 0.994), showing high sensitivity, with a lower detection limit of 0.008 ng mL−1, and good selectivity. The sensor’s effectiveness was also verified in real spiked wheat samples with satisfactory recoveries of 95.6–105.9%. The current work provides a flexible approach for the rapid and sensitive analysis of highly toxic DON in food samples and may also be easily extended to detect other hazardous substances with alternative target-recognition aptamers.
Xiaoyan Wen; Qingwen Huang; Dongxia Nie; Xiuying Zhao; Haojie Cao; Wenhui Wu; Zheng Han. A Multifunctional N-Doped Cu–MOFs (N–Cu–MOF) Nanomaterial-Driven Electrochemical Aptasensor for Sensitive Detection of Deoxynivalenol. Molecules 2021, 26, 2243 .
AMA StyleXiaoyan Wen, Qingwen Huang, Dongxia Nie, Xiuying Zhao, Haojie Cao, Wenhui Wu, Zheng Han. A Multifunctional N-Doped Cu–MOFs (N–Cu–MOF) Nanomaterial-Driven Electrochemical Aptasensor for Sensitive Detection of Deoxynivalenol. Molecules. 2021; 26 (8):2243.
Chicago/Turabian StyleXiaoyan Wen; Qingwen Huang; Dongxia Nie; Xiuying Zhao; Haojie Cao; Wenhui Wu; Zheng Han. 2021. "A Multifunctional N-Doped Cu–MOFs (N–Cu–MOF) Nanomaterial-Driven Electrochemical Aptasensor for Sensitive Detection of Deoxynivalenol." Molecules 26, no. 8: 2243.
The extensive exposure to multiple mycotoxins has been demonstrated in many countries; however, realistic assessments of the risks related to cumulative exposure are limited. This biomonitoring study was conducted to investigate exposure to 23 mycotoxins/metabolites and their determinants in 227 adults (aged 20–88 years) in the Yangtze River Delta, China. Eight mycotoxins were detected in 110 urine samples, and multiple mycotoxins co-occurred in 51/227 (22.47%) of urine samples, with deoxynivalenol (DON), fumonisin B1 (FB1), and zearalenone (ZEN) being the most frequently occurring. For single mycotoxin risk assessment, FB1, ZEN, aflatoxin B1 (AFB1), and ochratoxin A (OTA) all showed potential adverse effects. However, for the 12 samples containing DON and ZEN, in which none had a hazard risk, the combination of both mycotoxins in two samples was considered to pose potential endocrine disrupting risks to humans by hazard index (HI) method. The combined margin of exposure (MOET) for AFB1 and FB1 could constitute a potential health concern, and AFB1 was the main contributor. Our approach provides a blueprint for evaluating the cumulative risks related to different types of mycotoxins and opens a new horizon for the accurate interpretation of epidemiological health outcomes related to multi-mycotoxin exposure.
Qingwen Huang; Keqiu Jiang; Zhanmin Tang; Kai Fan; Jiajia Meng; Dongxia Nie; Zhihui Zhao; Yongjiang Wu; Zheng Han. Exposure Assessment of Multiple Mycotoxins and Cumulative Health Risk Assessment: A Biomonitoring-Based Study in the Yangtze River Delta, China. Toxins 2021, 13, 103 .
AMA StyleQingwen Huang, Keqiu Jiang, Zhanmin Tang, Kai Fan, Jiajia Meng, Dongxia Nie, Zhihui Zhao, Yongjiang Wu, Zheng Han. Exposure Assessment of Multiple Mycotoxins and Cumulative Health Risk Assessment: A Biomonitoring-Based Study in the Yangtze River Delta, China. Toxins. 2021; 13 (2):103.
Chicago/Turabian StyleQingwen Huang; Keqiu Jiang; Zhanmin Tang; Kai Fan; Jiajia Meng; Dongxia Nie; Zhihui Zhao; Yongjiang Wu; Zheng Han. 2021. "Exposure Assessment of Multiple Mycotoxins and Cumulative Health Risk Assessment: A Biomonitoring-Based Study in the Yangtze River Delta, China." Toxins 13, no. 2: 103.
A simple and reliable analytical method for the simultaneous determination of alternariol (AOH), altenuene (ALT), tentoxin (TEN), altenusin (ALS), tenuazonic acid (TeA), and alternariol monomethyl ether (AME) in grapes was developed by ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure with the extraction by acetonitrile and purification by sodium chloride (0.5 g) and anhydrous magnesium sulfate (0.5 g) was established to recover the six Alternaria toxins. After validation by determining the linearity (R2 > 0.99), recovery (77.8–101.6%), sensitivity (limit of detection in the range of 0.03–0.21 μg kg−1, and limit of quantification in the range of 0.09–0.48 μg kg−1), and precision (relative standard deviation (RSD) ≤ 12.9%), the analytical method was successfully applied to reveal the contamination state of Alternaria toxins in grapes. Among 56 grape samples, 40 (incidence of 71.4%) were contaminated with Alternaria toxins. TEN was the most frequently found mycotoxin (37.5%), with a concentration range of 0.10–1.64 μg kg−1, followed by TeA (28.6%) and AOH (26.8%). ALT (10.7%), AME (3.6%), and ALS (5.4%) were also detected in some samples. To the best of our knowledge, this is the first report about the Alternaria toxins contamination in grapes in China.
Wenbo Guo; Kai Fan; Dongxia Nie; Jiajia Meng; Qingwen Huang; Junhua Yang; Yuanyuan Shen; Emmanuel K. Tangni; Zhihui Zhao; Yongjiang Wu; Zheng Han. Development of a QuEChERS-Based UHPLC-MS/MS Method for Simultaneous Determination of Six Alternaria Toxins in Grapes. Toxins 2019, 11, 87 .
AMA StyleWenbo Guo, Kai Fan, Dongxia Nie, Jiajia Meng, Qingwen Huang, Junhua Yang, Yuanyuan Shen, Emmanuel K. Tangni, Zhihui Zhao, Yongjiang Wu, Zheng Han. Development of a QuEChERS-Based UHPLC-MS/MS Method for Simultaneous Determination of Six Alternaria Toxins in Grapes. Toxins. 2019; 11 (2):87.
Chicago/Turabian StyleWenbo Guo; Kai Fan; Dongxia Nie; Jiajia Meng; Qingwen Huang; Junhua Yang; Yuanyuan Shen; Emmanuel K. Tangni; Zhihui Zhao; Yongjiang Wu; Zheng Han. 2019. "Development of a QuEChERS-Based UHPLC-MS/MS Method for Simultaneous Determination of Six Alternaria Toxins in Grapes." Toxins 11, no. 2: 87.