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Prof. Marek Kieliszek
Faculty of Food Sciences, Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159 C, 02-776 Warsaw, Poland

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0 Biochemistry
0 Biotechnology
0 Cancer
0 Microbiology
0 Selenium

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Selenium
bacteria
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Review
Published: 12 August 2021 in Molecules
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Different parts of a plant (seeds, fruits, flower, leaves, stem, and roots) contain numerous biologically active compounds called “phytoconstituents” that consist of phenolics, minerals, amino acids, and vitamins. The conventional techniques applied to extract these phytoconstituents have several drawbacks including poor performance, low yields, more solvent use, long processing time, and thermally degrading by-products. In contrast, modern and advanced extraction nonthermal technologies such as pulsed electric field (PEF) assist in easier and efficient identification, characterization, and analysis of bioactive ingredients. Other advantages of PEF include cost-efficacy, less time, and solvent consumption with improved yields. This review covers the applications of PEF to obtain bioactive components, essential oils, proteins, pectin, and other important materials from various parts of the plant. Numerous studies compiled in the current evaluation concluded PEF as the best solution to extract phytoconstituents used in the food and pharmaceutical industries. PEF-assisted extraction leads to a higher yield, utilizes less solvents and energy, and it saves a lot of time compared to traditional extraction methods. PEF extraction design should be safe and efficient enough to prevent the degradation of phytoconstituents and oils.

ACS Style

Muhammad Modassar A. N. Ranjha; Rabia Kanwal; Bakhtawar Shafique; Rai Naveed Arshad; Shafeeqa Irfan; Marek Kieliszek; Przemysław Łukasz Kowalczewski; Muhammad Irfan; Muhammad Zubair Khalid; Ume Roobab; Rana Muhammad Aadil. A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents. Molecules 2021, 26, 4893 .

AMA Style

Muhammad Modassar A. N. Ranjha, Rabia Kanwal, Bakhtawar Shafique, Rai Naveed Arshad, Shafeeqa Irfan, Marek Kieliszek, Przemysław Łukasz Kowalczewski, Muhammad Irfan, Muhammad Zubair Khalid, Ume Roobab, Rana Muhammad Aadil. A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents. Molecules. 2021; 26 (16):4893.

Chicago/Turabian Style

Muhammad Modassar A. N. Ranjha; Rabia Kanwal; Bakhtawar Shafique; Rai Naveed Arshad; Shafeeqa Irfan; Marek Kieliszek; Przemysław Łukasz Kowalczewski; Muhammad Irfan; Muhammad Zubair Khalid; Ume Roobab; Rana Muhammad Aadil. 2021. "A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents." Molecules 26, no. 16: 4893.

Preprint content
Published: 27 July 2021
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Scientific interventions have identified lignocellulosic biomass as potential raw material for various industrial processes. However toxic byproducts released during the process result in deterioration of environment to a greater extent. Microbes can utilize these wastes for production of products of commercial value like bio-fuels, protein, organic acids and xylitol. However, high production cost and astringent operating conditions have been the major bottlenecks for its commercial production. In microbes, xylose is metabolized by xylose isomerase (XI) and xylose reductase-xylitol dehydrogenase (XR-XDH) pathways, with later having ability to transform pure xylose as well as xylose rich lignocelluloses. Efforts to find hyper producer isolates for xylitol production resulted in identification of one such isolate Pseudomonas gessardii VXlt-16 (MG770460) by 16s rDNA sequencing. Statistical optimization resulted in 7.28 folds’ increase in xylitol yield with 64.76% xylose bioconversion. Conversion of xylose to xylitol even at large scale suggests the possible application of bacterial isolate for the production of this useful product at industrial scale.

ACS Style

Vishal Ahuja; Sanjeev Mehta; Ranju Kumari Rathour; Vaishali Sharma; Nidhi Rana; Sheetal Sheetal; Arvind Kumar Bhatt; Marek Kieliszek. Elucidation of new xylose metabolizing pathway in Pseudomonas gessardii VXlt-16 and its correlation with xylitol production from sugarcane bagasse hydrolysate. 2021, 1 .

AMA Style

Vishal Ahuja, Sanjeev Mehta, Ranju Kumari Rathour, Vaishali Sharma, Nidhi Rana, Sheetal Sheetal, Arvind Kumar Bhatt, Marek Kieliszek. Elucidation of new xylose metabolizing pathway in Pseudomonas gessardii VXlt-16 and its correlation with xylitol production from sugarcane bagasse hydrolysate. . 2021; ():1.

Chicago/Turabian Style

Vishal Ahuja; Sanjeev Mehta; Ranju Kumari Rathour; Vaishali Sharma; Nidhi Rana; Sheetal Sheetal; Arvind Kumar Bhatt; Marek Kieliszek. 2021. "Elucidation of new xylose metabolizing pathway in Pseudomonas gessardii VXlt-16 and its correlation with xylitol production from sugarcane bagasse hydrolysate." , no. : 1.

Preprint content
Published: 12 July 2021
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Scientific interventions have identified lignocellulosic biomass as potential raw material for various industrial processes. However toxic byproducts released during the process result in deterioration of environment to a greater extent. Microbes can utilize these wastes for production of products of commercial value like bio-fuels, protein, organic acids and xylitol. However, high production cost and astringent operating conditions have been the major bottlenecks for its commercial production. In microbes, xylose is metabolized by xylose isomerase (XI) and xylose reductase-xylitol dehydrogenase (XR-XDH) pathways, with later having ability to transform pure xylose as well as xylose rich lignocelluloses. Efforts to find hyper producer isolates for xylitol production resulted in identification of one such isolate Pseudomonas gessardii VXlt-16 (MG770460) by 16s rDNA sequencing. Statistical optimization resulted in 7.28 folds’ increase in xylitol yield with 64.76% xylose bioconversion. Conversion of xylose to xylitol even at large scale suggests the possible application of bacterial isolate for the production of this useful product at industrial scale.

ACS Style

Vishal Ahuja; Sanjeev Mehta; Ranju Kumari Rathour; Vaishali Sharma; Nidhi Rana; Arvind Kumar Bhatt; Marek Kieliszek. Elucidation of New Xylose Metabolizing Pathway in Pseudomonas Gessardii VXlt-16 and its Correlation with Xylitol Production from Sugarcane Bagasse Hydrolysate. 2021, 1 .

AMA Style

Vishal Ahuja, Sanjeev Mehta, Ranju Kumari Rathour, Vaishali Sharma, Nidhi Rana, Arvind Kumar Bhatt, Marek Kieliszek. Elucidation of New Xylose Metabolizing Pathway in Pseudomonas Gessardii VXlt-16 and its Correlation with Xylitol Production from Sugarcane Bagasse Hydrolysate. . 2021; ():1.

Chicago/Turabian Style

Vishal Ahuja; Sanjeev Mehta; Ranju Kumari Rathour; Vaishali Sharma; Nidhi Rana; Arvind Kumar Bhatt; Marek Kieliszek. 2021. "Elucidation of New Xylose Metabolizing Pathway in Pseudomonas Gessardii VXlt-16 and its Correlation with Xylitol Production from Sugarcane Bagasse Hydrolysate." , no. : 1.

Review article
Published: 08 July 2021 in Fungal Biology Reviews
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The search for new, biotechnologically useful yeast strains has been carried out in many research centers in the world. Sporobolomyces and Sporidiobolus are examples of such useful yeasts, that can be used as a source of many valuable metabolites in industries. This article describes the modern taxonomy of these yeasts, which resulted from many years of research, including both classical microbiology and genetic analyses. Subsequently, the paper presents a review of scientific studies on the biosynthesis of various extracellular and intracellular metabolites produced by Sporobolomyces and Sporidiobolus yeasts, which are of great importance in the contemporary food, feed, and pharmaceutical industries. Such metabolites include exopolysaccharides, lipids, carotenoids, enzymes, and γ-decalactone. Aiming at developing a sustainable circular bioeconomy, this study considers two directions of use of these yeasts, i.e., as a feed additive and as an antagonist in the biocontrol of plant materials. This article is one of the first to organize the knowledge collected from published studies and present the contemporary scientific achievements and prospects for the biotechnological use of Sporobolomyces and Sporidiobolus yeasts.

ACS Style

Anna M. Kot; Marek Kieliszek; Kamil Piwowarek; Stanisław Błażejak; Cassamo Ussemane Mussagy. Sporobolomyces and Sporidiobolus – non-conventional yeasts for use in industries. Fungal Biology Reviews 2021, 37, 41 -58.

AMA Style

Anna M. Kot, Marek Kieliszek, Kamil Piwowarek, Stanisław Błażejak, Cassamo Ussemane Mussagy. Sporobolomyces and Sporidiobolus – non-conventional yeasts for use in industries. Fungal Biology Reviews. 2021; 37 ():41-58.

Chicago/Turabian Style

Anna M. Kot; Marek Kieliszek; Kamil Piwowarek; Stanisław Błażejak; Cassamo Ussemane Mussagy. 2021. "Sporobolomyces and Sporidiobolus – non-conventional yeasts for use in industries." Fungal Biology Reviews 37, no. : 41-58.

Journal article
Published: 05 July 2021 in Foods
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The purpose of this study was to evaluate the hydrocolloids–protein mixture as a fat replacer in sugar-free low-fat muffin cakes. In this study, a hydrocolloids mixture including konjac and guar gums and soy protein isolate (SPI) was applied to the cake. The combination of gums and SPI was named as mixture of stabilizers (MOS), and the treatments were designed using Design-Expert software and the response surface methodology (RSM) in order to optimize and reduce the oil content of muffin cakes by replacing it with MOS. Evaluation of treatments characteristics were investigated on days 1 and 15 of their production. The dependent variables were moisture content, water activity, specific volume, porosity, hardness, cohesiveness, springiness, chewiness and crumb color of cakes. The results show that increasing the percentage of MOS has positive effects on the final products in comparison to oil. In other words, an increase in the MOS content resulted in an increase in the moisture content, water activity, specific volume, height, springiness, cohesiveness, chewiness and L* (lightness) values, but negative effect on hardness, a* (redness) and b* (yellowness) values. As a result of optimizing using RSM, the usage of 4.08% oil and 0.31% MOS resulted in a 62.9% reduction in oil content in comparison with the control sample. The panelists assigned the lowest score to hardness and crumb color and the highest score to overall acceptability and cohesiveness to the optimized muffin.

ACS Style

Elahe Azmoon; Farzad Saberi; Fatemeh Kouhsari; Mehdi Akbari; Marek Kieliszek; Amir Vakilinezam. The Effects of Hydrocolloids-Protein Mixture as a Fat Replacer on Physicochemical Characteristics of Sugar-Free Muffin Cake: Modeling and Optimization. Foods 2021, 10, 1549 .

AMA Style

Elahe Azmoon, Farzad Saberi, Fatemeh Kouhsari, Mehdi Akbari, Marek Kieliszek, Amir Vakilinezam. The Effects of Hydrocolloids-Protein Mixture as a Fat Replacer on Physicochemical Characteristics of Sugar-Free Muffin Cake: Modeling and Optimization. Foods. 2021; 10 (7):1549.

Chicago/Turabian Style

Elahe Azmoon; Farzad Saberi; Fatemeh Kouhsari; Mehdi Akbari; Marek Kieliszek; Amir Vakilinezam. 2021. "The Effects of Hydrocolloids-Protein Mixture as a Fat Replacer on Physicochemical Characteristics of Sugar-Free Muffin Cake: Modeling and Optimization." Foods 10, no. 7: 1549.

Journal article
Published: 29 June 2021 in Molecules
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Propionic acid bacteria are the source of many metabolites, e.g., propionic acid and trehalose. Compared to microbiological synthesis, the production of these metabolites by petrochemical means or enzymatic conversion is more profitable. The components of microbiological media account for a large part of the costs associated with propionic fermentation, due to the high nutritional requirements of Propionibacterium. This problem can be overcome by formulating a medium based on the by-products of technological processes, which can act as nutritional sources and at the same time replace expensive laboratory preparations (e.g., peptone and yeast extract). The metabolic activity of P. freudenreichii was investigated in two different breeding environments: in a medium containing peptone, yeast extract, and biotin, and in a waste-based medium consisting of only apple pomace and potato wastewater. The highest production of propionic acid amounting to 14.54 g/L was obtained in the medium containing apple pomace and pure laboratory supplements with a yield of 0.44 g/g. Importantly, the acid production parameters in the waste medium reached almost the same level (12.71 g/L, 0.42 g/g) as the medium containing pure supplements. Acetic acid synthesis was more efficient in the waste medium; it was also characterized by a higher level of accumulated trehalose (59.8 mg/g d.s.). Thus, the obtained results show that P. freudenreichii bacteria exhibited relatively high metabolic activity in an environment with apple pomace used as a carbon source and potato wastewater used as a nitrogen source. This method of propioniate production could be cheaper and more sustainable than the chemical manner.

ACS Style

Kamil Piwowarek; Edyta Lipińska; Elżbieta Hać-Szymańczuk; Anna Maria Kot; Marek Kieliszek; Sylwia Bonin. Use of Propionibacterium freudenreichii T82 Strain for Effective Biosynthesis of Propionic Acid and Trehalose in a Medium with Apple Pomace Extract and Potato Wastewater. Molecules 2021, 26, 3965 .

AMA Style

Kamil Piwowarek, Edyta Lipińska, Elżbieta Hać-Szymańczuk, Anna Maria Kot, Marek Kieliszek, Sylwia Bonin. Use of Propionibacterium freudenreichii T82 Strain for Effective Biosynthesis of Propionic Acid and Trehalose in a Medium with Apple Pomace Extract and Potato Wastewater. Molecules. 2021; 26 (13):3965.

Chicago/Turabian Style

Kamil Piwowarek; Edyta Lipińska; Elżbieta Hać-Szymańczuk; Anna Maria Kot; Marek Kieliszek; Sylwia Bonin. 2021. "Use of Propionibacterium freudenreichii T82 Strain for Effective Biosynthesis of Propionic Acid and Trehalose in a Medium with Apple Pomace Extract and Potato Wastewater." Molecules 26, no. 13: 3965.

Journal article
Published: 31 May 2021 in LWT
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This study aimed to investigate the effect of alternative, non-wheat flours on the rheological properties of wafer batter as well as textural and sensory attributes of wafer sheet. The results indicated that the highest weight and moisture content were observed in wafers containing barley and oat flours. The addition of non-wheat flours other than maize (corn) flour led to a higher viscosity compared with the control batter. Wafer containing rye flour had the lowest hardness and the highest porosity (88.2%) among all samples. Non-wheat flours improve the nutritional value of the wafer; however, the addition of these flours to the wafer can change the rheological properties of the wafer batter and final product quality. Among the tested alternative non-wheat flours, maize flour showed the greatest similarity to wheat flour in terms of viscosity, which is the most important property of the wafer batter. According to the results, it is feasible to use non-wheat flours in a wafer with improved nutritional properties.

ACS Style

Mahshad Nasabi; Behnaz Naderi; Mehdi Akbari; Tugba Aktar; Marek Kieliszek; Mehdi Amini. Physical, structural and sensory properties of wafer batter and wafer sheets influenced by various sources of grains. LWT 2021, 149, 111826 .

AMA Style

Mahshad Nasabi, Behnaz Naderi, Mehdi Akbari, Tugba Aktar, Marek Kieliszek, Mehdi Amini. Physical, structural and sensory properties of wafer batter and wafer sheets influenced by various sources of grains. LWT. 2021; 149 ():111826.

Chicago/Turabian Style

Mahshad Nasabi; Behnaz Naderi; Mehdi Akbari; Tugba Aktar; Marek Kieliszek; Mehdi Amini. 2021. "Physical, structural and sensory properties of wafer batter and wafer sheets influenced by various sources of grains." LWT 149, no. : 111826.

Journal article
Published: 29 April 2021 in Applied Sciences
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In this study, we used Endomyces magnusii yeast with a complete respiratory chain and well-developed mitochondria system. This system is similar to the animal one which makes the yeast species an excellent model for studying ageing mechanisms. Mitochondria membranes play a vital role in the metabolic processes in a yeast cell. Mitochondria participate in the metabolism of several pivotal compounds including fatty acids (FAs) metabolism. The mitochondria respiratory activity, the membrane and storage lipids composition, and morphological changes in the culture during the long-lasting cultivation (for 168 h) were under investigation. High metabolic activity of E. magnusii might be related to the active function of mitochondria increasing in the 96- and 168-h growth phases. Cardiolipin (CL), phosphatidylethanolamine (PE), phosphatidylcholine (PC), and sterols (St) were dominant in the membrane lipids. The St and sphingolipids (SL) shares increased by a lot, whereas the CL and phosphatidylinositol (PI) + PE ones decreased in the membrane lipids. This was the main change in the membrane lipid composition during the cultivation. In contrast, the amount of PE and phosphatidylserine (PS) did not change. Index of Hydrogen Deficiency (IHD) of phospholipids (PL) FAs significantly declined due to a decrease in the linoleic acid share and an increase in the amount of palmitic and oleic acid. There were some storage lipids in the mitochondria where free fatty acids (FFAs) (73–99% of the total) dominated, reaching the highest level in the 96-h phase. Thus, we can conclude that upon long-lasting cultivation, for the yeast assimilating an “oxidative” substrate, the following factors are of great importance in keeping longevity: (1) a decrease in the IHD reduces double bonds and the peroxidation indices of various lipid classes; (2) the amount of long-chain FFAs declines. Moreover, the factor list providing a long lifespan should include some other physiological features in the yeast cell. The alternative oxidase activity induced in the early stationary growth phase and high mitochondria activity maintains intensive oxygen consumption. It determines the ATP production and physiological doses of reactive oxygen species (ROS), which could be regarded as a trend favoring the increased longevity.

ACS Style

Elena Isakova; Natalya Gessler; Daria Dergacheva; Vera Tereshina; Yulia Deryabina; Marek Kieliszek. Lipid Remodeling in the Mitochondria upon Ageing during the Long-Lasting Cultivation of Endomyces magnusii. Applied Sciences 2021, 11, 4069 .

AMA Style

Elena Isakova, Natalya Gessler, Daria Dergacheva, Vera Tereshina, Yulia Deryabina, Marek Kieliszek. Lipid Remodeling in the Mitochondria upon Ageing during the Long-Lasting Cultivation of Endomyces magnusii. Applied Sciences. 2021; 11 (9):4069.

Chicago/Turabian Style

Elena Isakova; Natalya Gessler; Daria Dergacheva; Vera Tereshina; Yulia Deryabina; Marek Kieliszek. 2021. "Lipid Remodeling in the Mitochondria upon Ageing during the Long-Lasting Cultivation of Endomyces magnusii." Applied Sciences 11, no. 9: 4069.

Review
Published: 21 April 2021 in Biological Trace Element Research
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Selenium (Se) is an important microelement with numerous positive effects on human health and diseases. It is important to specify that the status and consumption of Se are for a specific community as the levels of Se are extremely unpredictable between different populations and regions. Our existing paper was based on the impacts of Se on human health and disease along with data on the Se levels in Middle Eastern countries. Overall, the findings of this comprehensive review show that the consumption and levels of Se are inadequate in Middle Eastern nations. Such findings, together with the growing awareness of the importance of Se to general health, require further work primarily on creating an acceptable range of blood Se concentration or other measures to determine optimal Se consumption and, consequently, to guarantee adequate Se supplementation in populations at high risk of low Se intake.

ACS Style

Marek Kieliszek; Iqra Bano; Hamed Zare. A Comprehensive Review on Selenium and Its Effects on Human Health and Distribution in Middle Eastern Countries. Biological Trace Element Research 2021, 1 -17.

AMA Style

Marek Kieliszek, Iqra Bano, Hamed Zare. A Comprehensive Review on Selenium and Its Effects on Human Health and Distribution in Middle Eastern Countries. Biological Trace Element Research. 2021; ():1-17.

Chicago/Turabian Style

Marek Kieliszek; Iqra Bano; Hamed Zare. 2021. "A Comprehensive Review on Selenium and Its Effects on Human Health and Distribution in Middle Eastern Countries." Biological Trace Element Research , no. : 1-17.

Book chapter
Published: 05 April 2021 in Advances in Food and Nutrition Research
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Selenium (Se) is an element commonly found in the environment at different levels. Its compounds are found in soil, water, and air. This element is also present in raw materials of plant and animal origin, so it can be introduced into human organisms through food. Selenium is a cofactor of enzymes responsible for the antioxidant protection of the body and plays an important role in regulating inflammatory processes in the body. A deficiency in selenium is associated with a number of viral diseases, including COVID-19. This element, taken in excess, may have a toxic effect in the form of joint diseases and diseases of the blood system. Persistent selenium deficiency in the body may also impact infertility, and in such cases supplementation is needed.

ACS Style

Marek Kieliszek. Selenium. Advances in Food and Nutrition Research 2021, 96, 417 -429.

AMA Style

Marek Kieliszek. Selenium. Advances in Food and Nutrition Research. 2021; 96 ():417-429.

Chicago/Turabian Style

Marek Kieliszek. 2021. "Selenium." Advances in Food and Nutrition Research 96, no. : 417-429.

Review
Published: 25 March 2021 in Molecules
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Over the past several decades, we have observed a very rapid development in the biotechnological use of lactic acid bacteria (LAB) in various branches of the food industry. All such areas of activity of these bacteria are very important and promise enormous economic and industrial successes. LAB are a numerous group of microorganisms that have the ability to ferment sugars into lactic acid and to produce proteolytic enzymes. LAB proteolytic enzymes play an important role in supplying cells with the nitrogen compounds necessary for their growth. Their nutritional requirements in this regard are very high. Lactic acid bacteria require many free amino acids to grow. The available amount of such compounds in the natural environment is usually small, hence the main function of these enzymes is the hydrolysis of proteins to components absorbed by bacterial cells. Enzymes are synthesized inside bacterial cells and are mostly secreted outside the cell. This type of proteinase remains linked to the cell wall structure by covalent bonds. Thanks to advances in enzymology, it is possible to obtain and design new enzymes and their preparations that can be widely used in various biotechnological processes. This article characterizes the proteolytic activity, describes LAB nitrogen metabolism and details the characteristics of the peptide transport system. Potential applications of proteolytic enzymes in many industries are also presented, including the food industry.

ACS Style

Marek Kieliszek; Katarzyna Pobiega; Kamil Piwowarek; Anna Kot. Characteristics of the Proteolytic Enzymes Produced by Lactic Acid Bacteria. Molecules 2021, 26, 1858 .

AMA Style

Marek Kieliszek, Katarzyna Pobiega, Kamil Piwowarek, Anna Kot. Characteristics of the Proteolytic Enzymes Produced by Lactic Acid Bacteria. Molecules. 2021; 26 (7):1858.

Chicago/Turabian Style

Marek Kieliszek; Katarzyna Pobiega; Kamil Piwowarek; Anna Kot. 2021. "Characteristics of the Proteolytic Enzymes Produced by Lactic Acid Bacteria." Molecules 26, no. 7: 1858.

Review article
Published: 19 February 2021 in Trends in Food Science & Technology
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Microbial transglutaminase (MTGase) has been widely used to modify the functional properties of proteins in food systems. In the last 30 years since the discovery of MTGase, many efforts have been made on new strain isolation, culture media optimization, and fermentation procedure optimization to obtain MTGase with higher activity. Additionally, over the last decade, many studies have switched the focus from conventional optimization to genetic engineering in order to develop a highly efficient MTGase expression system with desired properties such as thermostability, activity, and yield by using genetic manipulation of strains such as Escherichia coli, Bacillus subtilis, and Pichia pastoris. In this review, we describe not only the recent advances and limitations related to MTGase biosynthesis but also the potential of MTGase for application in the food industry for some food products, including meat products, cheese, yogurt, and bread. Promoter engineering, gene codon optimization, signal peptide fusion, constitutive expression, random and rotational mutagenesis, etc. have been applied to enhance the recombinant expression system of MTGase. After three decades of research, the expression of recombinant MTGase has been significantly improved from the formation of inclusion body and enzyme with very low activity to the soluble form with high activity. Recombinant MTGase technology could also resolve problems related to post-translational modification in MTGase biosynthesis, resulting in facilitating downstream processing. In the future, it has been predicted that the scope of research will expand to work on heterologous expression by combination of genetic engineering tools. Further research is also needed to evaluate the biosynthesis of recombinant MTGase on a larger scale.

ACS Style

Mehdi Akbari; Seyed Hadi Razavi; Marek Kieliszek. Recent advances in microbial transglutaminase biosynthesis and its application in the food industry. Trends in Food Science & Technology 2021, 110, 458 -469.

AMA Style

Mehdi Akbari, Seyed Hadi Razavi, Marek Kieliszek. Recent advances in microbial transglutaminase biosynthesis and its application in the food industry. Trends in Food Science & Technology. 2021; 110 ():458-469.

Chicago/Turabian Style

Mehdi Akbari; Seyed Hadi Razavi; Marek Kieliszek. 2021. "Recent advances in microbial transglutaminase biosynthesis and its application in the food industry." Trends in Food Science & Technology 110, no. : 458-469.

Review
Published: 06 February 2021 in Metabolites
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Carotenoids are an essential group of compounds that may be obtained by microbiological synthesis. They are instrumental in various areas of industry, medicine, agriculture, and ecology. The increase of carotenoids’ demand at the global market is now essential. At the moment, the production of natural carotenoids is more expensive than obtaining their synthetic forms, but several new approaches/directions on how to decrease this difference were developed during the last decades. This review briefly describes the information accumulated until now about the beneficial effects of carotenoids on human health protection, their possible application in the treatments of various diseases, and their use in the food and feed industry. This review also describes some issues that are linked with biotechnological production of fungal and yeasts carotenoids, as well as new approaches/directions to make their biotechnological production more efficient.

ACS Style

Alexander Rapoport; Irina Guzhova; Lorenzo Bernetti; Pietro Buzzini; Marek Kieliszek; Anna Kot. Carotenoids and Some Other Pigments from Fungi and Yeasts. Metabolites 2021, 11, 92 .

AMA Style

Alexander Rapoport, Irina Guzhova, Lorenzo Bernetti, Pietro Buzzini, Marek Kieliszek, Anna Kot. Carotenoids and Some Other Pigments from Fungi and Yeasts. Metabolites. 2021; 11 (2):92.

Chicago/Turabian Style

Alexander Rapoport; Irina Guzhova; Lorenzo Bernetti; Pietro Buzzini; Marek Kieliszek; Anna Kot. 2021. "Carotenoids and Some Other Pigments from Fungi and Yeasts." Metabolites 11, no. 2: 92.

Journal article
Published: 01 January 2021 in Open Life Sciences
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This study demonstrated the effect of positional isomerism of benzoic acid derivatives against E. coli ATCC 700728 with the serotype O157. The addition of hydroxyl and methoxyl substituents weakened the effect of acids against E. coli with respect to benzoic acid (except 2-hydroxybenzoic). The connection of the hydroxyl group at the second carbon atom in the benzoic ring reduced the time needed to kill bacterial cells. Phenolic acids with methoxyl substitutes limited the biofilm formation by E. coli to a greater extent than hydroxyl derivatives. The most significant influence on the antibacterial activity of phenolic acids has the type of substituent attached to the benzoic ring, their number, and finally the number of carbon atoms at which the functional group is located.

ACS Style

Alicja Synowiec; Kinga Żyła; Małgorzata Gniewosz; Marek Kieliszek. An effect of positional isomerism of benzoic acid derivatives on antibacterial activity against Escherichia coli. Open Life Sciences 2021, 16, 594 -601.

AMA Style

Alicja Synowiec, Kinga Żyła, Małgorzata Gniewosz, Marek Kieliszek. An effect of positional isomerism of benzoic acid derivatives on antibacterial activity against Escherichia coli. Open Life Sciences. 2021; 16 (1):594-601.

Chicago/Turabian Style

Alicja Synowiec; Kinga Żyła; Małgorzata Gniewosz; Marek Kieliszek. 2021. "An effect of positional isomerism of benzoic acid derivatives on antibacterial activity against Escherichia coli." Open Life Sciences 16, no. 1: 594-601.

Journal article
Published: 13 December 2020 in Metabolites
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The production of biomolecules using agro-industrial by-products as feedstock is a growing trend worldwide. Selenium (Se) is a trace element essential for health, and the Se-enrichment of yeast biomass can enhance its benefits. This study investigated the feasibility of the production of Saccharomyces cerevisiae Se-enriched biomass using a medium composed of corn bran and soybean bran acid hydrolysates as carbon and nitrogen sources in a stirred-tank reactor. After hydrolysis, hydrolysates presented complex composition and high concentrations of sugars, proteins, and minerals. The use of a stirred-tank bioreactor leads to the production of 9 g/L S. cerevisiae biomass enriched with 236.93 μg/g Se, and 99% cell viability. Likewise, the combination of sugarcane molasses and soybean bran hydrolysate was effective for cell growth of a probiotic strain of S. cerevisiae with a 24.08% β-glucan content. The results demonstrated that starchy acid hydrolysates are low-cost and efficient substrates for the production of yeast biomass and derivate products and may contribute to further studies for a sustainable development of biorefinery technologies.

ACS Style

Sabrina Evelin Martiniano; Letícia Alves Fernandes; Edith Mier Alba; Rafael Rodrigues Philippini; Stephanie Caroline Tavares Tabuchi; Marek Kieliszek; Júlio César Dos Santos; Silvio Silvério Da Silva. A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor. Metabolites 2020, 10, 508 .

AMA Style

Sabrina Evelin Martiniano, Letícia Alves Fernandes, Edith Mier Alba, Rafael Rodrigues Philippini, Stephanie Caroline Tavares Tabuchi, Marek Kieliszek, Júlio César Dos Santos, Silvio Silvério Da Silva. A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor. Metabolites. 2020; 10 (12):508.

Chicago/Turabian Style

Sabrina Evelin Martiniano; Letícia Alves Fernandes; Edith Mier Alba; Rafael Rodrigues Philippini; Stephanie Caroline Tavares Tabuchi; Marek Kieliszek; Júlio César Dos Santos; Silvio Silvério Da Silva. 2020. "A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor." Metabolites 10, no. 12: 508.

Journal article
Published: 09 November 2020 in Scientific Reports
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In this study, we aimed to perform structural and proteomic analysis of the vitelline membrane (VM) of two species birds belonging to the family Turdidae: blackbird (Turdus merula) and song thrush (Turdus philomelos). We performed structural analyses using scanning electron microscopy. The VM proteins were identified and compared to the best-known chicken VM proteins. According to our results, VM of both species has a typical three-layered structure: the outer layer, inner layer, and the continuous membrane between them. An unusual observation was the finding of “convexity” formed by the inner layer in blackbird. The role of these convex structures is not known, but they can be typical for the species and can be used in their identification. In addition, we identified two proteins in the VM of both species of birds, of which U3KEZ1 FICAL was not previously identified in any other bird species, and the U3JXV8 FICAL protein was confirmed only once in cockatiel parrot VM. The function of these proteins is not exactly known, but their structure shows similarities to the SERPIN proteins that are involved in microbiological defense, i.e., they are immune proteins. This study contributes to the current knowledge about the structure and composition of proteins of VM, especially because similar analyses have never been performed for Turdidae family. Knowledge of the structure and specific proteins of blackbird and song thrush VM can be beneficial in research on ecology and bird biology and also helpful in developing noninvasive and nongenetic identification methods.

ACS Style

Krzysztof Damaziak; Marek Kieliszek; Dariusz Gozdowski. Structural and proteomic analyses of vitelline membrane proteins of blackbird (Turdus merula) and song thrush (Turdus philomelos). Scientific Reports 2020, 10, 1 -14.

AMA Style

Krzysztof Damaziak, Marek Kieliszek, Dariusz Gozdowski. Structural and proteomic analyses of vitelline membrane proteins of blackbird (Turdus merula) and song thrush (Turdus philomelos). Scientific Reports. 2020; 10 (1):1-14.

Chicago/Turabian Style

Krzysztof Damaziak; Marek Kieliszek; Dariusz Gozdowski. 2020. "Structural and proteomic analyses of vitelline membrane proteins of blackbird (Turdus merula) and song thrush (Turdus philomelos)." Scientific Reports 10, no. 1: 1-14.

Review article
Published: 22 October 2020 in Open Life Sciences
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Cellular biomass of microorganisms can be effectively used in the treatment of waste from various branches of the agro-food industry. Urbanization processes and economic development, which have been intensifying in recent decades, lead to the degradation of the natural environment. In the first half of the 20th century, problems related to waste management were not as serious and challenging as they are today. The present situation forces the use of modern technologies and the creation of innovative solutions for environmental protection. Waste of industrial origin are difficult to recycle and require a high financial outlay, while the organic waste of animal and plant origins, such as potato wastewater, whey, lignin, and cellulose, is dominant. In this article, we describe the possibilities of using microorganisms for the utilization of various waste products. A solution to reduce the costs of waste disposal is the use of yeast biomass. Management of waste products using yeast biomass has made it possible to generate new metabolites, such as β-glucans, vitamins, carotenoids, and enzymes, which have a wide range of industrial applications. Exploration and discovery of new areas of applications of yeast, fungal, and bacteria cells can lead to an increase in their effective use in many fields of biotechnology.

ACS Style

Marek Kieliszek; Kamil Piwowarek; Anna M. Kot; Katarzyna Pobiega. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry. Open Life Sciences 2020, 15, 787 -796.

AMA Style

Marek Kieliszek, Kamil Piwowarek, Anna M. Kot, Katarzyna Pobiega. The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry. Open Life Sciences. 2020; 15 (1):787-796.

Chicago/Turabian Style

Marek Kieliszek; Kamil Piwowarek; Anna M. Kot; Katarzyna Pobiega. 2020. "The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry." Open Life Sciences 15, no. 1: 787-796.

Journal article
Published: 28 September 2020 in Applied Sciences
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Current industry needs are related to higher awareness of modern consumers. These consumers are looking for products in which properties such as bioactive compounds are preserved as much as possible. Plasma treatment is one of the most promising nonthermal technologies that can decontaminate food and keep its original properties. Therefore, the aim of this work was to examine the usefulness of atmospheric pressure argon microwave plasma on decontamination of black pepper seeds, allspice berries and juniper berries. The samples were irradiated by plasma for 15–60 s and their physicochemical (dry matter content, water activity, color, total phenolic content, antioxidant activity, piperine content in black pepper seeds) and microbial (bacteria and molds count) quality was evaluated afterwards. Results demonstrated that plasma irradiation for 15 s was sufficient for partial inactivation of A. niger but less effective against the Gram-positive bacterium B. subtilis, regardless of the raw material. At the same time, plasma treatment reduced water activity, which can positively affect further storage of spices. Properly selected plasma parameters may also enhance extractability of phenolics or piperine (from black pepper seeds) and improve antioxidant activity with not very great, but visible, color changes.

ACS Style

Artur Wiktor; Bartosz Hrycak; Mariusz Jasiński; Katarzyna Rybak; Marek Kieliszek; Karolina Kraśniewska; Dorota Witrowa-Rajchert. Impact of Atmospheric Pressure Microwave Plasma Treatment on Quality of Selected Spices. Applied Sciences 2020, 10, 6815 .

AMA Style

Artur Wiktor, Bartosz Hrycak, Mariusz Jasiński, Katarzyna Rybak, Marek Kieliszek, Karolina Kraśniewska, Dorota Witrowa-Rajchert. Impact of Atmospheric Pressure Microwave Plasma Treatment on Quality of Selected Spices. Applied Sciences. 2020; 10 (19):6815.

Chicago/Turabian Style

Artur Wiktor; Bartosz Hrycak; Mariusz Jasiński; Katarzyna Rybak; Marek Kieliszek; Karolina Kraśniewska; Dorota Witrowa-Rajchert. 2020. "Impact of Atmospheric Pressure Microwave Plasma Treatment on Quality of Selected Spices." Applied Sciences 10, no. 19: 6815.

Journal article
Published: 25 July 2020 in International Journal of Molecular Sciences
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Selenium (Se) was found to inhibit the growth of the yeast Candida utilis ATCC 9950. Cells cultured in 30 mg selenite/L supplemented medium could bind 1368 µg Se/g of dry weight in their structures. Increased accumulation of trehalose and glycogen was observed, which indicated cell response to stress conditions. The activity of antioxidative enzymes (glutathione peroxidase, glutathione reductase, thioredoxin reductase, and glutathione S-transferase) was significantly higher than that of the control without Se addition. Most Se was bound to water-insoluble protein fraction; in addition, the yeast produced 20–30 nm Se nanoparticles (SeNPs). Part of Se was metabolized to selenomethionine (10%) and selenocysteine (20%). The HPLC-ESI-Orbitrap MS analysis showed the presence of five Se compounds combined with glutathione in the yeast. The obtained results form the basis for further research on the mechanisms of Se metabolism in yeast cells.

ACS Style

Marek Kieliszek; Katarzyna Bierla; Javier Jiménez-Lamana; Anna Maria Kot; Jaime Alcántara-Durán; Kamil Piwowarek; Stanisław Błażejak; Joanna Szpunar. Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium. International Journal of Molecular Sciences 2020, 21, 5287 .

AMA Style

Marek Kieliszek, Katarzyna Bierla, Javier Jiménez-Lamana, Anna Maria Kot, Jaime Alcántara-Durán, Kamil Piwowarek, Stanisław Błażejak, Joanna Szpunar. Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium. International Journal of Molecular Sciences. 2020; 21 (15):5287.

Chicago/Turabian Style

Marek Kieliszek; Katarzyna Bierla; Javier Jiménez-Lamana; Anna Maria Kot; Jaime Alcántara-Durán; Kamil Piwowarek; Stanisław Błażejak; Joanna Szpunar. 2020. "Metabolic Response of the Yeast Candida utilis During Enrichment in Selenium." International Journal of Molecular Sciences 21, no. 15: 5287.

Article
Published: 06 July 2020 in Biological Trace Element Research
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Nowadays, there is an increase attention on the effect of selenium (Se) on metabolic processes of microorganisms. Strains belonging to the genus of Yarrowia are of great biotechnological interest for various industries. In this study, we evaluated the effect of 10 mg/L of Se on the growth and lipid production of two Yarrowia lipolytica strains: the ACA DC 50109 and one more with increased oleagenicity, derived after ALE methodology (referred here as Y. lipolytica ALE_70). The presence of Se in the growth medium negatively affected both cell mass production and total lipid accumulation, for both Y. lipolytica strains. Fractionation of total lipids showed an inhibition on neutral lipid (NL) synthesis and consequently, an increase of polar lipids (glycolipids plus sphingolipids, and phospholipids) on the lipids of the Se-enriched ACA DC 50109 strain; however, the NL/polar ratio of the Se-enriched ALE_70 indicated that Se, apart from the inhibition of NL synthesis, provoked also the accumulation of polar lipids in this strain. In addition, the fatty acid (FA) composition was differently affected by Se. Se-enriched total lipids of the ALE_70 strain were enriched in linoleic acid (C18:2 n-6), which resulted in increase of the unsaturated index. On the other hand, Se-enriched lipids of the ACA DC 50109 strain were more saturated, as the percentage of palmitic (C16:0) and stearic (C18:0) acids increased in the total FAs. Moreover, it seems that Se influenced the activity or the expression of desaturases and elongase in both strains. Finally, the supplementation of growth medium with Se affected cell morphology, as well as the size and distribution of lipid droplets inside the yeast cells. According to our opinion, Se caused stress conditions and the consequence of that was the occurrence of metabolic disorders that affected cell mass, lipid content, and/or morphological structures. The results of the present study suggest that further research should be carried out to understand the background of the lipogenesis process in yeast cells cultured under stress conditions.

ACS Style

Marek Kieliszek; Marianna Dourou. Effect of Selenium on the Growth and Lipid Accumulation of Yarrowia lipolytica Yeast. Biological Trace Element Research 2020, 199, 1611 -1622.

AMA Style

Marek Kieliszek, Marianna Dourou. Effect of Selenium on the Growth and Lipid Accumulation of Yarrowia lipolytica Yeast. Biological Trace Element Research. 2020; 199 (4):1611-1622.

Chicago/Turabian Style

Marek Kieliszek; Marianna Dourou. 2020. "Effect of Selenium on the Growth and Lipid Accumulation of Yarrowia lipolytica Yeast." Biological Trace Element Research 199, no. 4: 1611-1622.