Soil microbial communities play a crucial role in maintaining diverse ecosystem functions within the saline-alkali soil ecosystems. Therefore, in this study, we collected various saline-alkaline soils from across the Inner Mongolia Hetao irrigation area. The soil chemical properties were analyzed, and the microbial diversity of bacteria and fungi was measured using 16 S rRNA and ITS rRNA amplicon sequencing. The dynamic relationship between the soil microbial community and soil factors was analyzed using the ABT (Aggregate Enhanced tree) model, the co-occurrence network, and the structural equation model. The results indicated that electrical conductivity (EC) was the biggest driving force of various saline-alkaline soils, affecting the community structure of bacteria (22.80%) and fungi (21.30%). The soil samples were categorized into three treatment levels based on their EC values: the low-salinity group (L, EC: 0–1 ms/cm, n = 10), the medium-salinity group (M, EC: 1–2 ms/cm, n = 8), and the high-salinity group (H, EC > 2 ms/cm, n = 6). The results demonstrated a negative correlation between microbial abundance and salinity-alkalinity, while revealing an enhanced interrelationship among species. The alterations in bacterial (12.36%) and fungal (22.92%) communities in various saline-alkali soils were primarily driven by saline-alkali ions, which served as the principal direct factors. The negative correlation between EC and SOM exhibited the highest magnitude, whereas the positive correlation between soil organic carbon and EC demonstrated the greatest strength. Therefore, it was further substantiated that EC played a pivotal role in shaping the distinct microbial communities in saline-alkali soils.

Meteorological factors are one of the important factors that determine maize kernel weight and grain nutritional quality. Analyzing the influence of meteorological factors before and after anthesis on maize kernel weight and nutritional quality components is of great significance for improving corn yield and quality. Therefore, five different maize hybrids and conducted continuous experiments from 2018 to 2021 were selected in this study, to explore the response of maize kernel weight and grain nutritional quality to meteorological factors in different growth periods, and to quantify the linear relationship between grain nutritional quality parameters, grain weight, and meteorological factors. The main results were as follows: the 100-grain weight reached the maximum value of 39.53 g in 2018; the contents of crude protein, total starch, and crude fat in grains reached the maximum in 2018, 2020, and 2018, respectively, which were 9.61%, 69.2%, and 5.1%. Meteorological factors significantly affected the maize grain weight (p < 0.05). Before anthesis, total sunshine duration, average temperature, relative humidity, and the accumulated temperature had strong effects on grain weight. After anthesis, average daily temperature, total rainfall, temperature difference, accumulated temperature, average daily highest temperature, and total sunshine hours had strong effects on grain weight. There was also a significant correlation between grain weight and grain nutritional quality components (p < 0.05). The multivariate polynomial equation analysis revealed that further potential for maize grain weight could be exploited by adjusting the content of each quality component of the kernels under the current test conditions. Meteorological elements can indirectly affect the 100-grain weight through their relationship with the nutritional quality of the grains, with accumulated temperature before anthesis, average temperature after anthesis, and accumulated temperature after anthesis having the greatest indirect effect on the 100-grain weight. Therefore, the effects of pre-anthesis accumulation temperature, post-anthesis average temperature, and post-anthesis accumulation temperature on the nutritional quality of the grains can be harmonized by the application of hybrids of different lengths of vegetation and by adjusting the sowing time in agricultural production. Ultimately, maize grain weight can be increased on the basis of optimizing the content of various quality components in the grains.

Corn straw is an important renewable resource, which could improve the quality of saline–alkali cultivated land. However, the slow decomposition of crop residues in cold, arid, and saline–alkali soils can lead to serious resource waste and ecological crises. The use of beneficial microorganisms with degradation functions could solve these problems. In this study, three types of saline–alkali soil with low, medium, and high salinity levels were used in the straw-returning experiment. The experiment was conducted with four treatments: GF2 (Klebsiella sp.), GF7 (Pseudomonas sp.), GF2+GF7, and CK (control without bacteria). Microbial characteristics, straw degradation efficiency, element release rate, and soil factors were compared, and random forest linear regression and partial least squares path modeling analysis methods were utilized. The results indicated that the degradation of bacterial metabolites, the efficiency of corn stover degradation, the efficiency of component degradation, and the release rates of elements (C, N, P, and K) initially increased and then decreased with the increase in salinity. At the maximum value of moderately saline–alkali soil, the effect of GF2+GF7 treatment was significantly better than that of other treatments (p < 0.05). Given the interactive effects of saline–alkali soil and microbial factors, the application of exogenous degrading bacteria could significantly increase soil enzyme activity and soil available nutrients, as well as regulate the salt–alkali ion balance in soil. The cation exchange capacity (9.13%, p < 0.01) was the primary driving force for the degradation rate of straw in saline–alkali soil with different degrees of salinization under the influence of exogenous degrading bacteria. Straw decomposition directly affected the soil chemical properties and indirectly affected soil enzyme activity. The results of this study would provide new strategies and insights into the utilization of microbial resources to promote straw degradation.

Maize is essential in ensuring food security in China as a primary food and feed crop. One of the main ways to increase yield in maize production systems is to increase planting density as appropriate. Clarifying the relationship between planting density and grain nutritional quality can provide theoretical guidance for high-yielding high-quality maize cultivation and management practices. To this end, five representative high-yielding maize varieties from the 1970s to the 2010s in China were used as experimental material, and two planting densities of 45,000 plants hm⁻² and 105,000 plants hm⁻² were set to analyze the changing patterns of yield traits and grain nutrient quality of maize varieties in different eras, as well as their responses to densification conditions. The results showed that, under low-density conditions, the grain nutrient quality components of the 2010s’ variety (DH618) were all different 75 days after anthesis compared with the 1970s’–2000s’ varieties and yields also significantly increased by 11.15% to 19.18% (p < 0.05). The increase in planting density led to a rise in total grain starch and soluble sugar content 75 days post-anthesis in all varieties from the 1970s to the 2010s, with increases of 0.65–1.65% and 39.44–69.01%, and a decrease in crude grain protein and crude fat content, with reductions of 4.15–8.50% and 3.00–11.18%. The increase in total grain starch content 75 days post-anthesis was mainly due to the rise in grain starch accumulation between 23 and 47 days post-anthesis in the 1970s’–2010s’ varieties, with an increase of 7.72–9.19% in all varieties. The higher accumulation of crude fat and soluble sugar in the 0–23 days post-anthesis period also contributed to the increase in total starch accumulation in the 23–47 days post-anthesis period. Ultimately, densification conditions also contributed to a significant increase in yield across all eras of the varieties based on changes in grain nutritional quality, with a more significant increase in yield due to densification and a smaller decrease in grain crude fat content due to densification 75 days after anthesis in the 2010s’ variety (DH618). Therefore, in cultivation and production processes that do not have specific requirements for the nutritional quality components of maize grain, we suggest that the use of a representative high-yielding maize variety (DH618) from the 2010s, together with appropriate planting at close planting distances, can significantly increase maize yields based on an increase in the total starch content of the grain at physiological maturity.

The increasing salinization of cultivated soil worldwide has led to a significant reduction in maize production. Using saline–alkaline-tolerant growth-promoting bacteria (PGPR) in the rhizosphere can significantly improve the saline tolerance of maize and ensure the stability of maize yields, which has become a global research hotspot. This study screened salt-tolerant microorganisms Klebsiella sp. (GF2) and Pseudomonas sp. (GF7) from saline soil to clarify the mechanism in improving the saline tolerance of maize. In this study, different application treatments (GF2, GF7, and GF2 + GF7) and no application (CK) were set up to explore the potential ecological relationships between the saline tolerance of maize seedlings, soil characteristics, and microorganisms. The results showed that co-occurrence network and Zi-Pi analysis identified Klebsiella and Pseudomonas as core microbial communities in the rhizosphere soil of maize seedlings grown in saline soil. The deterministic process of microbial assembly mainly controlled the bacterial community, whereas bacteria and fungi were governed by random processes. The application of saline–alkaline-resistant PGPR under saline stress significantly promoted maize seedling growth, increased the activity of soil growth-promoting enzymes, and enhanced total nitrogen, soil organic carbon, and microbial carbon and nitrogen contents. Additionally, it reduced soil salt and alkali ion concentrations [electrical conductivity (EC) and exchangeable Na⁺]. Among them, GF2 + GF7 treatment had the best effect, indicating that saline–alkaline-tolerant PGPR could directly or indirectly improve the saline tolerance of maize seedlings by improving the rhizosphere soil ecological environment. EC was the determining factor to promote maize seedling growth under saline–alkaline stress (5.56%; p < 0.01). The results provided an important theoretical reference that deciphers the role of soil factors and microecology in enhancing the saline tolerance of maize.

Introduction: Straw return combined with rational nitrogen (N) fertilization plays a critical role in coordinating the transformation of soil organic carbon and nitrogen availability, thereby improving nitrogen use efficiency (NUE), crop yield, and soil fertility. However, the dynamics of soil carbon and nitrogen fractions under straw return with varying N inputs, and their specific contributions to NUE and yield, remain unclear.Methods: A three-year split-plot field experiment was conducted in the Tumochuan Plain Irrigation District. The main plots included deep plowing with straw return (DPR) and no straw return (RT), while subplots comprised four N application rates (0, 210, 255, and 300 kg ha^-1). Soil carbon and nitrogen fractions, maize yield, NUE, and partial factor productivity of nitrogen (PFPN) were assessed.Results: Compared to RT, DPR significantly improved soil nutrient levels and labile C and N fractions in the 0–40 cm soil layer. Maize yield, NUE, and PFPN increased by 17.28%, 18.24%, and 17.88%, respectively. Under DPR, a linear-plus-plateau model estimated the optimal N rate at 237.3 kg ha^-1, reducing N input by 20.89% without compromising performance. Key contributors to NUE and PFPN included mineral nitrogen (MN), soil quality index (SQI), and dry matter accumulation (DMA), with relative contributions of 9.39%, 8.96%, and 8.49% to NUE, and 9.31%, 9.18%, and 8.99% to PFPN, respectively.Discussion: Straw return enhanced soil nitrogen availability and maize productivity by improving MN and SQI. Even with a 15–20% reduction in N application, DPR sustained high soil C and N fractions, yield, and NUE. These results offer practical guidance for optimizing N management under long-term straw return, with significant implications for sustainable maize production and soil fertility enhancement.