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Ms. Diana Cruz-Pulido
Food Animal Health Research Program (FAHRP)

Basic Info

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Research Keywords & Expertise

0 Infectious Diseases
0 Microbiology & Host Interaction
0 coronaviruses
0 Genomics and molecular biology
0 Bioinformatics applications integrating RNA-Seq with other NGS data types

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Short Biography

Diana Cruz-Pulido has a Bachelor's degree in Microbiology and she earned two Masters degrees: Biological Sciences at Universidad de Los Andes (Bogotá, Colombia), and Comparative and Veterinary Medicine at the Ohio State University (Columbus, Ohio, USA). Currently, she is a Graduate Research Associate at OSU/OARDC Food Animal Health Research Program, in Wooster, Ohio, USA. Her fields of interest are Bioinformatics and Virology. For her Ph.D program, Diana is working on understanding infection and host-switch mechanisms of Coronaviruses.

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Journal article
Published: 13 February 2021 in Viruses
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Porcine deltacoronavirus (PDCoV) is an emerging infectious disease of swine with zoonotic potential. Phylogenetic analysis suggests that PDCoV originated recently from a host-switching event between birds and mammals. Little is known about how PDCoV interacts with its differing hosts. Human-derived cell lines are susceptible to PDCoV infection. Herein, we compare the gene expression profiles of an established host swine cells to potential emerging host human cells after infection with PDCoV. Cell lines derived from intestinal lineages were used to reproduce the primary sites of viral infection in the host. Porcine intestinal epithelial cells (IPEC-J2) and human intestinal epithelial cells (HIEC) were infected with PDCoV. RNA-sequencing was performed on total RNA extracted from infected cells. Human cells exhibited a more pronounced response to PDCoV infection in comparison to porcine cells with more differentially expressed genes (DEGs) in human, 7486, in comparison to pig cells, 1134. On the transcriptional level, the adoptive host human cells exhibited more DEGs in response to PDCoV infection in comparison to the primary pig host cells, where different types of cytokines can control PDCoV replication and virus production. Key immune-associated DEGs and signaling pathways are shared between human and pig cells during PDCoV infection. These included genes related to the NF-kappa-B transcription factor family, the interferon (IFN) family, the protein-kinase family, and signaling pathways such as the apoptosis signaling pathway, JAK-STAT signaling pathway, inflammation/cytokine–cytokine receptor signaling pathway. MAP4K4 was unique in up-regulated DEGs in humans in the apoptosis signaling pathway. While similarities exist between human and pig cells in many pathways, our research suggests that the adaptation of PDCoV to the porcine host required the ability to down-regulate many response pathways including the interferon pathway. Our findings provide an important foundation that contributes to an understanding of the mechanisms of PDCoV infection across different hosts. To our knowledge, this is the first report of transcriptome analysis of human cells infected by PDCoV.

ACS Style

Diana Cruz-Pulido; Patricia A. Boley; Wilberforce Zachary Ouma; Moyasar Alhamo; Linda J. Saif; Scott P. Kenney. Comparative Transcriptome Profiling of Human and Pig Intestinal Epithelial Cells after Porcine Deltacoronavirus Infection. Viruses 2021, 13, 292 .

AMA Style

Diana Cruz-Pulido, Patricia A. Boley, Wilberforce Zachary Ouma, Moyasar Alhamo, Linda J. Saif, Scott P. Kenney. Comparative Transcriptome Profiling of Human and Pig Intestinal Epithelial Cells after Porcine Deltacoronavirus Infection. Viruses. 2021; 13 (2):292.

Chicago/Turabian Style

Diana Cruz-Pulido; Patricia A. Boley; Wilberforce Zachary Ouma; Moyasar Alhamo; Linda J. Saif; Scott P. Kenney. 2021. "Comparative Transcriptome Profiling of Human and Pig Intestinal Epithelial Cells after Porcine Deltacoronavirus Infection." Viruses 13, no. 2: 292.

Comparative study
Published: 01 January 2012 in BMC Microbiology
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Klebsiella pneumoniae can be found in environmental habitats as well as in hospital settings where it is commonly associated with nosocomial infections. One of the factors that contribute to virulence is its capacity to form biofilms on diverse biotic and abiotic surfaces. The second messenger Bis-(3’-5’)-cyclic dimeric GMP (c-di-GMP) is a ubiquitous signal in bacteria that controls biofilm formation as well as several other cellular processes. The cellular levels of this messenger are controlled by c-di-GMP synthesis and degradation catalyzed by diguanylate cyclase (DGC) and phophodiesterase (PDE) enzymes, respectively. Many bacteria contain multiple copies of these proteins with diverse organizational structure that highlight the complex regulatory mechanisms of this signaling network. This work was undertaken to identify DGCs and PDEs and analyze the domain structure of these proteins in K. pneumoniae.

ACS Style

Diana P Cruz; Mónica G Huertas; Marcela Lozano; Lina Zárate; María M Zambrano. Comparative analysis of diguanylate cyclase and phosphodiesterase genes in Klebsiella pneumoniae. BMC Microbiology 2012, 12, 139 -139.

AMA Style

Diana P Cruz, Mónica G Huertas, Marcela Lozano, Lina Zárate, María M Zambrano. Comparative analysis of diguanylate cyclase and phosphodiesterase genes in Klebsiella pneumoniae. BMC Microbiology. 2012; 12 (1):139-139.

Chicago/Turabian Style

Diana P Cruz; Mónica G Huertas; Marcela Lozano; Lina Zárate; María M Zambrano. 2012. "Comparative analysis of diguanylate cyclase and phosphodiesterase genes in Klebsiella pneumoniae." BMC Microbiology 12, no. 1: 139-139.