Symptoms of leaf blight, stem canker, and pod rot were observed onT. cacaoduring a series of samplings conducted in several states of Malaysia from September 2018 to March 2019. The identity of the pathogen that was responsible for the diseases was determined using morphological characteristics, DNA sequences, and phylogenetic analyses of multiple genes, namely, internal transcribed spacer (ITS), elongation translation factor 1-alpha (tef1-α), β-tubulin (tub2), and RNA polymerase subunit II (rpb2). A total of 57 isolates recovered from diseased leaves ofT. cacao(13 isolates), stems (20 isolates), and pods (24 isolates) showed morphological features that resembledLasiodiplodiasp. The identity of the isolates was further determined up to the species level by comparing DNA sequences and phylogenetic analyses of multiple genes. The phylogenetic analysis of the combined dataset of ITS,tef1-α,tub2, andrpb2elucidated that all of the isolates obtained wereLasiodiplodia theobromaeas supported by 97% bootstrap value. The results of pathogenicity tests revealedL. theobromaeas the causal pathogen of leaf blight, stem canker, and pod rot ofT. cacao.

Aims To identify Botryosphaeriaceae fungal species that are associated with stem‐end rot of mango, and to study their pathogenicity on mango fruit. Methods and Results Based on the sequences of ITS, TEF1‐α, and β‐tubulin, as well as on the phylogenetic analysis of combined sequences, four species of Lasiodiplodia (L. theobromae, L. pseudotheobromae, L. iranensis, L. mahajangana) and two species of Neofusicoccum (N. ribis, N. parvum) were identified. Pseudofusicoccum violaceum, Neoscytalidium dimidiatum and three species of Botryosphaeria (B. scharifii, B. dothidea, B. ramosa) were identified based on sequences of ITS and TEF1‐α. Pathogenicity test of selected isolates were tested on Chok Anan, Waterlily, and Falan mango cultivars. Generally, all species were observed to be pathogenic on the three tested mango cultivars on wounded fruits, except for N. ribis and N. parvum, which were pathogenic on both wounded and unwounded fruits. However, N. ribis was only pathogenic on cultivar Falan, whereas B. ramosa were pathogenic on cultivars Waterlily and Falan. Conclusions Eleven species of Botryosphaeriaceae were associated with mango stem‐end rot in Malaysia. To the best of our knowledge, four species, namely L. mahajangana, B. ramosa, N. ribis, and P. violaceum are the first recorded Botryosphaeriaceae fungi associated with stem end rot of mango. Significance and Impact of the Study The identification of Botryosphaeriaceae fungi is important to establish suitable control measures and quarantine requirements. Many species have a wide host range, which means that there is a possibility of cross infection from other infected plants.