Chronological age estimation is a challenging marker in the field of forensic medicine. The current study aimed to investigate the accuracy of signal joint T-cell receptor rearrangement excision ci...

Animal feed significantly influences the content and quality of animal products consumed by humans and plays a crucial role in the food chain. To simultaneously analyze 345 pesticide residues in poultry feed, a modified Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method was developed, utilizing gas chromatography-tandem mass spectrometry (GC-MS/MS). Validation studies were conducted with spiked pesticide standards, and commercial poultry feed samples were analyzed. The linearity of the QuEChERS method was evaluated, showing high coefficients of determination (R² ≥0.995) for all analytes. The method demonstrated excellent linearity (R² ≥0.995), recovery rates (86.8-109.2%), and precision (RSD ≤14.8%), meeting SANTE criteria. Limits of detection (LOD) ranged from 0.5 to 5 ng/kg and limits of quantification (LOQ) from 1 to 10 ng/kg, enhancing the method's reliability. Analysis of the poultry feed revealed that 15 out of the 345 pesticide residues were detected in the samples, with concentrations ranging from 2.1 to 120 µg/kg. The detected pesticide categories included insecticides, fungicides, plant growth regulators, and herbicides, in that order. In conclusion, the QuEChERS method combined with the GC-MS/MS approach provides a reliable technique for the multi-residue analysis of pesticides in complex feed matrices.

Objective: This study aimed to adequately investigate the most frequent causes of poisoning in camels and sheep in the Kingdom of Saudi Arabia (KSA). Methods: Appropriate data were carefully collected from the recorded animal poisoning cases in the Veterinary Health and Monitoring Department, Ministry of Environment, Water and Agriculture (MEWA), during the period (from 2018 to 2019). All recorded cases were accurately diagnosed with a case history and reported clinical signs as well as definitive confirmation by laboratory analysis of the collected samples. Results: From the recorded data of 852 camel and sheep poisoned cases, the results showed that the primary causes of animal poisoning in Saudi Arabia were Cyanogenic glycosides (42.25%), Pesticides (26.76%), Mycotoxins (18.8%), and Drugs (12.2%). These cases were recorded only in 5 geographical regions (Riyadh, Qassim, Makkah, Northern borders, and Eastern province) Conclusion: Accidental toxicity in camel and sheep in KSA was mainly caused by Cyanogenic glycosides, Pesticides, Mycotoxins, and Drug toxicity. The frequent reasons for possible toxicity should be minimized through active veterinary extension.