Maize is an excellent nutritional source and is consumed as a staple food in different parts of the world, including India. Developing a maize genotype with a combination of higher lysine and tryptophan, along with β-carotene, can help alleviate the problem of protein-energy malnutrition (PEM) and vitamin A deficiency (VAD). This study is aimed at improving lysine and tryptophan content by transferring opaque-2 (o2) gene from donor HKI163 to β-carotene-rich inbred lines viz., UMI1200β⁺ and UMI1230β⁺. For this purpose, F₁, BC₁F₁, BC₂F₁, BC₂F₂, and BC₂F₃ plants were developed using an o2 line HKI163 and two β-carotene-rich inbred lines, UMI1200β⁺ and UMI1230β⁺, as the parents. Foreground selection using the associated marker umc1066 for the o2 gene and the marker crtRB1 3′TE for the crtRB1 gene was used to select the target genes. A total of 236 simple sequence repeat (SSR) markers distributed evenly across the maize genome were employed for the background selection. To fix the crtRB1 allele in the BC₁F₁ stage, individual plants homozygous at the crtRB1 locus and heterozygous at the o2 locus were selected and used for backcrossing to produce BC₂F₁ plants. Furthermore, the selected heterozygous BC₂F₁ plants from both crosses were selfed to obtain the BC₂F₂ plants, which were then selected for the target gene and selfed to generate the BC₂F₃ lines. From each cross, five improved lines with homozygous marker alleles for the crtRB1 and o2 genes with a recurrent parent genome (RPG) recovery ranging from 86.75 to 91.21% in UMI1200β⁺×HKI163 and 80.00 to 90.08% in UMI1230β⁺×HKI163 were identified. The improved lines had good agronomic performance and possessed high lysine (0.294–0.332%), tryptophan (0.073–0.081%), and β-carotene (6.12–7.38 µg/g) content. These improved lines can be used as genetic resources for maize improvement.

The ‘Saltol’ QTL, responsible for salt tolerance at seedling stage of rice was successfully introgressed into ADT 43, a salt sensitive but a popular commercial rice variety of favorable irrigated rice ecosystem of Tamil Nadu, through Marker Assisted Backcross Breeding (MABB) technique. The newly developed ‘Saltol’ lines were confirmed to be salt tolerant, high yielding and with good cooking quality traits. The importance of RM 3412, RM 8094 and SKC 2a was envisaged in diagnosing the sodiicty tolerance. Role of other QTLs like qSES and qCHL on chromosome 3, in conferring sodicity tolerance at seeding stage was also indicated.

Maize is an important agricultural plant valued for its productivity and nutritive qualities. However, a deficiency of two essential amino acids (lysine and tryptophan) significantly reduces the nutritional quality of maize proteins. The recessive opaque-2 (o2) mutant has a greater content of lysine and tryptophan in their endosperm proteins and their bioavailability is better. Therefore, marker-assisted backcross breeding (MABB) was attempted to incorporate the o2 allele from the donor line VQL 1 into the genetic background of UMI 1200 to develop quality protein maize (QPM) lines. Foreground selection for the gene o2 was effected using tightly linked molecular marker umc1066, in UMI 1200 × VQL 1 backcross series. Further, background selection was done together with stringent phenotypic selection for agronomic traits, to accelerate recurrent parent phenome recovery. As a result, three advanced QPM maize lines (DBT 4-1-1/25-10/25-10/25-16, DBT 4-1-1/25-10/25-17/25-11 and DBT 4-1-1/25-10/25-17/25-13) carrying the opaque-2 allele in a homozygous state, similar to VQL 1 were developed. The lysine and tryptophan content of these lines ranged from 0.35% to 0.40% and 0.03 to 0.05%, respectively, which is on par with the VQL 1. Background analysis using 250 simple sequence repeats (SSRs) revealed up to 97% recurrent parent genome recovery. In conclusion, the newly developed QPM lines can be used in future maize breeding programmes to improve the nutritional traits.