Novel polymer nanowires with triple hydrogen-bonding sites have been prepared by a one-pot procedure based on metallogel template polymerization, which was carried out in an Ag(I)-coordinated organogel with benzoyl peroxide (BPO) as initiator. The product has been characterized using infrared spectroscopy, scanning electron microscopy and transmission electron microscopy. The experimental results reveal that the gel fiber is a crucial template for polymerization. Due to the degradation of the template in the polymerization, nanofibers of metallogel were transcribed to polymer nanowires. Polymer nanowires synthesized by this method contain triple hydrogen-bonding sites introduced by the monomer diacryloyl-2,6-diaminopyridine (DADAP); they are used as adsorbing agents for the ‘uptake’ of thymidine from dilute THF solution. The efficient adsorbing performance and reusable properties will render them ideal candidates for applications relating to biological adsorption.