Metformin is the first-line option for treating newly diagnosed diabetic patients and also involved in other pharmacological actions, including antitumor effect, anti-aging effect, polycystic ovarian syndrome prevention, cardiovascular action, and neuroprotective effect, etc. However, the mechanisms of metformin actions were not fully illuminated. Recently, increasing researches showed that autophagy is a vital medium of metformin playing pharmacological actions. Nevertheless, results on the effects of metformin on autophagy were inconsistent. Apart from few clinical evidences, more data focused on kinds of no-clinical models. First, many studies showed that metformin could induce autophagy via a number of signaling pathways, including AMPK-related signaling pathways (e.g. AMPK/mTOR, AMPK/CEBPD, MiTF/TFE, AMPK/ULK1, and AMPK/miR-221), Redd1/mTOR, STAT, SIRT, Na+/H+ exchangers, MAPK/ERK, PK2/PKR/AKT/ GSK3β, and TRIB3. Secondly, some signaling pathways were involved in the process of metformin inhibiting autophagy, such as AMPK-related signaling pathways (AMPK/NF-κB and other undetermined AMPK-related signaling pathways), Hedgehog, miR-570-3p, miR-142-3p, and MiR-3127-5p. Thirdly, two types of signaling pathways including PI3K/AKT/mTOR and endoplasmic reticulum (ER) stress could bidirectionally impact the effectiveness of metformin on autophagy. Finally, multiple signal pathways were reviewed collectively in terms of affecting the effectiveness of metformin on autophagy. The pharmacological effects of metformin combining its actions on autophagy were also discussed. It would help better apply metformin to treat diseases in term of mediating autophagy.

L-asparaginase (E.C.3.5.1.1) hydrolyzes L-asparagine to L-aspartic acid and ammonia, which has been widely applied in the pharmaceutical and food industries. Microbes have advantages for L-asparaginase production, and there are several commercially available forms of L-asparaginase, all of which are derived from microbes. Generally, L-asparaginase has an optimum pH range of 5.0–9.0 and an optimum temperature of between 30 and 60 °C. However, the optimum temperature of L-asparaginase from hyperthermophilic archaea is considerable higher (between 85 and 100 °C). The native properties of the enzymes can be enhanced by using immobilization techniques. The stability and recyclability of immobilized enzymes makes them more suitable for food applications. This current work describes the classification, catalytic mechanism, production, purification, and immobilization of microbial L-asparaginase, focusing on its application as an effective reducer of acrylamide in fried potato products, bakery products, and coffee. This highlights the prospects of cost-effective L-asparaginase, thermostable L-asparaginase, and immobilized L-asparaginase as good candidates for food application in the future.

Background: Smartphone addiction has become a social problem that affects the healthy growth of adolescents, and it is frequently reported to be correlated with self-esteem, self-control, and social support among adolescents.Methods: A meta-analysis was conducted by searching the PubMed, Web of Science, Embase, PsycINFO, PsycArticles, China National Knowledge Infrastructure (CNKI), WANFANG DATA, and Chongqing VIP Information Co., Ltd. (VIP) databases. Stata 16.0 was used to analyse the overall effect and test the moderating effect.Results: Fifty-six studies were included, involving a total of 42,300 participants. Adolescents' smartphone addiction had a moderately negative correlation with self-esteem (r = −0.25, 95% CI = −0.29 to −0.22, p < 0.001), a strong negative correlation with self-control (r = −0.48, 95% CI = −0.53 to −0.42, p < 0.001), and a weak negative correlation with social support (r = −0.16, 95% CI = −0.23 to −0.09, p < 0.001). Moderation analysis revealed that the correlation between adolescents' smartphone addiction and self-esteem was strongest when smartphone addiction was measured with the Mobile Phone Addiction Tendency Scale for College Students (MPATS; r = −0.38). The correlation between adolescents' smartphone addiction and self-control was strongest when self-control was measured with the Middle school students' Self-control Ability Questionnaire (MSAQ; r = −0.62). The effect of dissertations on smartphone addiction, self-control, and social support among adolescents was significantly larger than that of journal articles. The correlation between adolescents' smartphone addiction and social support was strongest when smartphone addiction was measured with the Mobile Phone Addiction Index (MPAI; r = −0.24). However, the correlations between adolescents' smartphone addiction and self-esteem, self-control, and social support were not affected by age or gender.Conclusion: There was a strong relationship between smartphone addiction and self-esteem, self-control, and social support among adolescents. In the future, longitudinal research should be carried out to better investigate the dynamic changes in therelationship between smartphone addiction and self-esteem, self-control, and social support.Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42022300061.