In recent years, the nanofluids (NFs) have become the main candidates for improving or even replacing traditional heat transfer fluids. The possibility of NFs to be used in various technological applications, from renewable energies to nanomedicine, has made NFs and their thermal conductivity one of the most studied topics nowadays. Hence, this review presents an overview of the most important advances and controversial results related to the NFs thermal conductivity. The different techniques used to measure the thermal conductivity of NFs are discussed. Moreover, the fundamental parameters that affect the NFs thermal conductivity are analyzed, and possible improvements are addressed, such as the increase of long-term stability of the nanoparticles (NPs).The most representative prediction classical models based on fluid mechanics, thermodynamics, and experimental fittings are presented. Also, the recent statistical machine learning-based prediction models are comprehensively addressed, and the comparison with the classical empirical ones is made, whenever possible.

In this study, a numerical approach was carried out to analyze the effects of different geometries of microchannel heat sinks on the forced convective heat transfer in single-phase flow. The simulations were performed using the commercially available software COMSOLMultiphysics 5.6^® (Burlington, MA, USA) and its results were compared with those obtained from experimental tests performed in microchannel heat sinks of polydimethylsiloxane (PDMS). Distilled water was used as the working fluid under the laminar fluid flow regime, with a maximum Reynolds number of 293. Three sets of geometries were investigated: rectangular, triangular and circular. The different configurations were characterized based on the flow orientation, type of collector and number of parallel channels. The main results show that the rectangular shaped collector was the one that led to a greater uniformity in the distribution of the heat transfer in the microchannels. Similar results were also obtained for the circular shape. For the triangular geometry, however, a disturbance in the jet impingement was observed, leading to the least uniformity. The increase in the number of channels also enhanced the uniformity of the flow distribution and, consequently, improved the heat transfer performance, which must be considered to optimize new microchannel heat sink designs. The achieved optimized design for a heat sink, with microchannels for nanofluid flow and a higher heat dissipation rate, comprised a rectangular collector with eight microchannels and vertical placement of the inlet and outlet.

The root causes for the mechanisms for margination and near-wall platelet accumulation have previously been investigated by numerical simulations and the results pointed to two main contributors. First, the behavior of the red blood cells (RBCs), namely, traveling around the centerline and formation of the cell-free layer (CFL). Second, the multiple interactions between RBCs and platelets. However, these mechanisms remain to be experimentally verified. In this work, we focus on the dynamics of platelet-size particles inside RBC flows through straight-square microchannels. We used rigid particles with nominal diameter of $2.47\mu \mathrm{m}$ to mimic platelets. The three-dimensional (3D) coordinates ($x,y,z$) and velocity components ($v_x,v_y,v_z$) of the platelet-size particles inside the RBC-suspension flows were measured by means of the general defocusing particle tracking method (GDPT). From the 3D particle trajectories, we were able to investigate the root causes for margination and near-wall particle accumulation. The overall picture points to the RBCs as the dictator of the near-wall particle accumulation and margination. We show that the phenomenon of margination is an irreversible, fast, unpredictable and discontinuous process, and more importantly it can be an opportunity-based event.

In this work, we provide a systematic theoretical and experimental characterization of bias errors in defocusing particle tracking (DPT) methods based on a single calibration function, with respect to microfluidic applications, in which it is not possible to use calibration targets inside the measurement volume. This approach is widely used in microfluidic experiments, but bias errors are often neglected and to date only few works reported empirical procedures to compensate for that. A systematic characterization of the impact of such error in DPT measurements is still lacking. We show that the field curvature aberration and the refractive index mismatch are the main sources of bias error in these applications. We present a correction methodology for the bias error based on the determination of a reference surface, and in addition we propose a procedure based on a reference measurement of a Poiseuille flow to determine the reference surface on microfluidic channels with constant cross section. We discuss the impact of the refractive index mismatch and how to correctly compensate for it. We validated our methodology and quantified the bias errors on 10 different experimental setups, using different working fluids, materials, geometries, and microscope objective lenses ranging from 5 $$\times$$× to 40 $$\times$$× magnification. Our results indicate that the impact of this type of bias errors is in general not predictable and must be evaluated case by case. The proposed methodology allows to estimate and minimize the bias error in most microfluidic setups and is suitable for any single-camera DPT approach.

The dielectric-barrier discharge plasma actuator is a well-established device commonly operated in boundary-layer airflows for active flow control. In the present experimental investigation, their ability to cause momentum transfer to the surrounding fluid is analyzed by means of spatio-temporal body-force distributions in both quiescent air and external airflow conditions. The work is motivated by the limitation to quiescent-air operating conditions of frequent previous efforts. Available analytical velocity-information-based force derivation approaches are contrasted to investigate the actuator performance under conditions of their area of application. Results of body force in quiescent air, in agreement with literature, confirm the major taken assumption for Navier–Stokes-based body-force formulations—a negligible pressure gradient. However, the previous circumstance turns out as an invalid assumption for plasma actuation encountering an external airflow. These outcomes coincide with the findings in the numerical work of (2015 Numerical investigation of plasma-actuator force-term estimations from flow experiments J. Phys. D: Appl. Phys. 48 395203), following the recommendation to apply a vorticity-equation-based approach under such conditions. Furthermore, the shape of the spatio-temporal body-force distribution is observed to undergo changes when the airflow speed increases. On the other hand, the integral force magnitude is found to remain approximately constant. Moreover, the choice of phase resolution of the discharge cycle has an implication on the accuracy of the temporal force evolution, therefore, clarifying the importance of a priori defining the type of body-force analysis in an experiment; i.e. integral force magnitude, time-averaged or time-resolved evaluation. As a promising finding of utmost importance for the actuator performance, the actuator remains as effective as in quiescent air under presence of the external airflow, which immediately renders the actuator fluid-mechanic efficiency to increase for increasing airflow speed.

Experimental investigations on the motion of rigid particles in microcirculation environments are still scarce owing to the three-dimensional (3D) motion of the particles and to the particle image masking due to the presence of the red blood cells (RBCs). Despite the recent progress on the 3D tracking of rigid particles in RBC flows with defocus particle tracking (DPT) methods, the problem of particle image masking remains to be solved. Here, we propose, test, and evaluate the use hemoglobin-free RBCs, also known as ghost RBCs, as a replacement for normal RBCs in experiments with rigid particles in microcirculation environments. We performed DPT measurements of a pressure-driven flow of normal and ghost RBC suspensions seeded with rigid particles at three different flow rates. We show that the quasi-transparent appearance of ghost RBCs, as a result of the lack of hemoglobin, eliminates the RBC-induced masking of the defocused particle images and allows to achieve the particle matching standards found in cell-free experiments. In fact, ghost RBC suspensions enable the tracking of the rigid particles across the entire height of the microchannel, which was not possible in normal RBC flows. On a fluid dynamic level, we show that ghost RBC suspensions provide similar conditions to normal RBCs in terms of the velocity of the rigid particles and the rigid particles exhibit similar lateral dynamics in both types of cell suspensions. In essence, the findings from this work demonstrate that ghost RBCs are a well-suited replacement for normal RBCs in experiments aiming at deciphering the motion of rigid particles in microcirculation environments. Graphical abstract