Biological filaments driven by molecular motors tend to experience tangential propulsive forces also known as active follower forces. When such a filament encounters an obstacle, it deforms, which reorients its follower forces and alters its entire motion. If the filament pushes a cargo, the friction on the cargo can be enough to deform the filament, thus affecting the transport properties of the cargo. Motivated by cytoskeletal filament motility assays, we study the dynamic buckling instabilities of a two-dimensional slender elastic filament driven through a dissipative medium by tangential propulsive forces in the presence of obstacles or cargo. We observe two distinct instabilities. When the filament’s head is pinned or experiences significant translational but little rotational drag from its cargo, it buckles into a steadily rotating coiled state. When it is clamped or experiences both significant translational and rotational drag from its cargo, it buckles into a periodically beating, overall translating state. Using minimal analytically tractable models, linear stability theory and fully nonlinear computations, we study the onset of each buckling instability, characterize each buckled state, and map out the phase diagram of the system. Finally, we use particle-based Brownian dynamics simulations to show our main results are robust to moderate noise and steric repulsion. Overall, our results provide a unified framework to understand the dynamics of tangentially propelled filaments and filament-cargo assemblies.

Polyacrylamide hydrogels (PAH gel) and polydimethylsiloxane (PDMS, an elastomer) are two soft materials often used in cell mechanics and mechanobiology, in manufacturing lab-on-a-chip applications, among others. This is partly due to the ability to tune their elasticity with ease in addition to various chemical modifications. For affine polymeric networks, two (of three) elastic constants, Young's modulus (E), the shear modulus (G), and Poisson's ratio (ν), describe the purely elastic response to external forces. However, the literature addressing the experimental determination of ν for PAH (sometimes called PAA gels in the literature) and the PDMS elastomer is surprisingly limited when compared to the literature that reports values of the elastic moduli, E and G. Here, we present a facile method to obtain the Poisson's ratio and Young's modulus for PAH gel and PDMS elastomer based on static tensile tests. The value of ν obtained from the deformation of the sample is compared to the value determined by comparing E and G via a second independent method that utilizes small amplitude shear rheology. We show that the Poisson's ratio may vary significantly from the value for incompressible materials (ν = 0.5), often assumed in the literature even for soft compressible hydrogels. Surprisingly, we find a high degree of agreement between elastic constants obtained by shear rheology and macroscopic static tension test data for polyacrylamide hydrogels but not for elastomeric PDMS.

The adaptive mechanical properties of soft and fibrous biological materials are relevant to their functionality. The emergence of the macroscopic response of these materials to external stress and intrinsic cell traction from local deformations of their structural components is not well understood. Here, we investigate the nonlinear elastic behavior of blood clots by combining microscopy, rheology, and an elastic network model that incorporates the stretching, bending, and buckling of constituent fibrin fibers. By inhibiting fibrin cross-linking in blood clots, we observe an anomalous softening regime in the macroscopic shear response as well as a reduction in platelet-induced clot contractility. Our model explains these observations from two independent macroscopic measurements in a unified manner, through a single mechanical parameter, the bending stiffness of individual fibers. Supported by experimental evidence, our mechanics-based model provides a framework for predicting and comprehending the nonlinear elastic behavior of blood clots and other active biopolymer networks in general.

Arrays of clamped active filaments, interacting via steric forces, display traveling waves and jammed clusters.

Swarming colonies of the light-responsive bacteria Serratia marcescens grown on agar exhibit robust fluctuating large-scale flows that include arrayed vortices, jets and sinuous streamers. We study the immobilization and quenching of these collective flows when the moving swarm is exposed to intense wide-spectrum light with a substantial ultraviolet component. We map the emergent response of the swarm to light in terms of two parameters—light intensity and duration of exposure—and identify the conditions under which collective motility is impacted. For small exposure times and/or low intensities, we find collective motility to be negligibly affected. Increasing exposure times and/or intensity to higher values suppresses collective motility but only temporarily. Terminating exposure allows bacteria to recover and eventually reestablish collective flows similar to that seen in unexposed swarms. For long exposure times or at high intensities, exposed bacteria become paralysed and form aligned, jammed regions where macroscopic speeds reduce to zero. The effective size of the quenched region increases with time and saturates to approximately the extent of the illuminated region. Post-exposure, active bacteria dislodge immotile bacteria; initial dissolution rates are strongly dependent on duration of exposure. Based on our experimental observations, we propose a minimal Brownian dynamics model to examine the escape of exposed bacteria from the region of exposure. Our results complement studies on planktonic bacteria, inform models of patterning in gradated illumination and provide a starting point for the study of specific wavelengths on swarming bacteria.

Elastic-driven slender filaments subjected to compressive follower forces provide a synthetic way to mimic the oscillatory beating of biological flagella and cilia. Here, we use a continuum model to study the dynamical, nonlinear buckling instabilities that arise due to the action of nonconservative follower forces on a prestressed slender rod clamped at both ends and allowed to move in a fluid. Stable oscillatory responses are observed as a result of the interplay between the structural elastic instability of the inextensible slender rod, geometric constraints that control the onset of instability, energy pumped into the system by the active follower forces, and motion-driven fluid dissipation. Initial buckling instabilities are initiated by the effect of the follower forces and inertia; fluid drag subsequently allows for the active energy pumped into the system to be dissipated away and results in self-limiting amplitudes. By integrating the equations of equilibrium and compatibility conditions with linear constitutive laws, we compute the critical follower forces for the onset of oscillations, emergent frequencies of these solutions, and the postcritical nonlinear rod shapes for two forms of the drag force, namely linear Stokes drag and quadratic Morrison drag. For a rod with fixed inertia and drag parameters, the minimum (critical) force required to initiate stable oscillations depends on the initial slack and weakly on the nature of the drag force. Emergent frequencies and the amplitudes postonset are determined by the extent of prestress as well as the nature of the fluid drag. Far from onset, for large follower forces, the frequency of the oscillations can be predicted by evoking a power balance between the energy input by the active forces and the dissipation due to fluid drag.