Vesicular stomatitis virus (VSV) emerges periodically from its focus of endemic transmission in southern Mexico to cause epizootics in livestock in the US. The ecology of VSV involves a diverse, but largely undefined, repertoire of potential reservoir hosts and invertebrate vectors. As part of a larger program to decipher VSV transmission, we conducted a study of the spatiotemporal dynamics of Simulium black flies, a known vector of VSV, along the Rio Grande in southern New Mexico, USA from March to December 2020. Serendipitously, the index case of VSV-Indiana (VSIV) in the USA in 2020 occurred at a central point of our study. Black flies appeared soon after the release of the Rio Grande’s water from an upstream dam in March 2020. Two-month and one-year lagged precipitation, maximum temperature, and vegetation greenness, measured as Normalized Difference Vegetation Index (NDVI), were associated with increased black fly abundance. We detected VSIV RNA in 11 pools comprising five black fly species using rRT-PCR; five pools yielded a VSIV sequence. To our knowledge, this is the first detection of VSV in the western US from vectors that were not collected on premises with infected domestic animals.

We present a summary and analysis of the Diptera-related information published in Zootaxa from 2001 to 2020, with a focus on taxonomic papers. Altogether, 2,527 papers on Diptera were published, including 2,032 taxonomic papers and 1,931 papers containing new nomenclatural acts, equivalent to 22% of all publications with new nomenclatural acts for Diptera. The new nomenclatural acts include 7,431 new species, 277 new genera, 2,003 new synonymies, and 1,617 new combinations. A breakdown by family of new taxa and new replacement names proposed in the journal during the last two decades is provided, together with a comparison of Zootaxa’s output to that of all other taxonomic publications on Diptera. Our results show that the journal has contributed to 20% of all biodiversity discovery in this megadiverse insect order over the last 20 years, and to about 31% in the last decade.

Adults of Aposycorax chilensis were collected from several sites during fieldwork in Chilean Patagonia, December 2013. Specimens were swept or aspirated from roadside seeps and found in greatest numbers during the morning hours. DNA was extracted from a recently blood-fed female and was subjected to the polymerase chain reaction using vertebrate-specific 16S primers. An amplicon was obtained and the resulting sequence was found to have over 99% identity with two frogs in the genus Batrachyla, thus establishing this species’ preference for amphibian hosts. The diagnosis and description of adult A. chilensis are revised, including the first description of the complete male genital tract. Habitat characteristics for this species and rotation of the male genitalia are discussed.