Antibiotic (Σsulfonamide, Σfluoroquinolone, Σtetracycline and Σmacrolide) contamination in aquatic systems has remained a global concern in recent years. In this study, fourteen antibiotics, sulfadiazine (SDZ), sulphapyridine (SPD), sulfacetamide (SCT), sulfamethoxazole (SMX), sulfamethazine (SMZ), trimethoprim (TMP), ofloxacin (OFX), lomefloxacin (LFX), ciprofloxacin (CFX), norfloxacin (NFX), oxytetracycline (OTC), tetracycline (TC), dehydroerythromycin (ETM-H2O) and roxithromycin (RTM), were analysed in zooplankton collected from the Yelang Lake Reservoir between May 2016 and Mar. 2017. Our results demonstrated that the antibiotic TC (mean 95.80 ng g-1), OTC (mean 167.0 ng g-1) and RTM (mean 151.80 ng g-1) concentrations were significantly higher in zooplankton than the other antibiotics. TC, OTC and RTM accumulated intensively in zooplankton, and the BAFs of OTC, RTM and TC were significantly higher, with mean BAF values of 78.43, 108.4 and 110.1, respectively. Antibiotic concentrations were strongly correlated with zooplankton phytoplankton biomass, OTC, RTM and SMX concentrations in zooplankton were significantly positively correlated with bacillariophyte biomass, and SDZ and SMZ concentrations in zooplankton were significantly positively correlated with T. brevifurcatus, T. mongolicus, and D. pulex biomass. SPD with P. tunguidus, C. cornuta, TMP, ETM and OFX with P. tunguidus, C. cornuta, M. leuckarti, C. vicinus vicinus, CFX with D. pulex, LFX with T. brevifurcatus, T. mongolicus, Bosmina longirostris, and D. dubium were significantly positively correlated. These results show that bioaccumulation or scavenging of antibiotics across trophic levels is a fundamental and complex component of antibiotic cycling in aquatic environments.