Dengue virus (DENV) is the causative pathogen in the life-threatening dengue hemorrhagic fever and dengue shock syndrome. DENV is transmitted to humans via the bite of an infected Aedes mosquito. Approximately 100 million people are infected annually worldwide, and most of those live in tropical and subtropical areas. There is still no effective drug or vaccine for treatment of DENV infection. In this study, we set forth to investigate the effect of melatonin, which is a natural hormone with multiple pharmacological functions, against DENV infection. Treatment with subtoxic doses of melatonin dose-dependently inhibited DENV production. Cross-protection across serotypes and various cell types was also observed. Time-of-addition assay suggested that melatonin exerts its influence during the post-entry step of viral infection. The antiviral activity of melatonin partly originates from activation of the sirtuin pathway since co-treatment with melatonin and the sirtuin 1 (SIRT1) inhibitor reversed the effect of melatonin treatment alone. Moreover, melatonin could modulate the transcription of antiviral genes that aid in suppression of DENV production. This antiviral mechanism of melatonin suggests a possible new strategy for treating DENV infection.

The Bio-Circular-Green (BCG) economy framework, which integrates economic, social, and environmental aspects for waste management, holds great promise for fostering growth across various sectors in Thailand. Venipuncture, a critical medical procedure, can lead to complications if not performed correctly. This study uses Thailand para latex resources and calcium carbonate extracted from recycled mollusk shells to develop an educational innovation model for venipuncture procedures. Natural rubber latex (NRL) was obtained from Chiang Rai, Thailand. Preserved latex was heated to obtain 60% concentrated latex, then combined with a sulfur solution, and calcium carbonate powder was added in various ratios. Molding used a specialized mold replicating anatomical features of the antecubital fossa, incorporating three prominent veins. The venipuncture practice rubber model was characterized for pH, moisture content, water absorption, tensile strength, and other properties. The model exhibited neutral pH, low moisture content, and notable resistance and elasticity. This study promotes the sustainable utilization of natural latex resources and recycled mollusk shells in developing innovative medical models within the BCG framework. Additional research is required to enhance the human friendliness and realism of the rubber material used in the venipuncture practice model.